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Q80YQ2

- MED23_MOUSE

UniProt

Q80YQ2 - MED23_MOUSE

Protein

Mediator of RNA polymerase II transcription subunit 23

Gene

Med23

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity. Also required for transcriptional activation subsequent to the assembly of the preinitiation complex. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.By similarity4 Publications

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_198602. PPARA activates gene expression.
    REACT_206529. Generic Transcription Pathway.
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 23
    Alternative name(s):
    Cofactor required for Sp1 transcriptional activation subunit 3
    Short name:
    CRSP complex subunit 3
    Mediator complex subunit 23
    Protein sur-2 homolog
    Short name:
    mSur-2
    Gene namesi
    Name:Med23
    Synonyms:Crsp3, Kiaa1216, Sur2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1917458. Med23.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: MGI
    2. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13671367Mediator of RNA polymerase II transcription subunit 23PRO_0000305931Add
    BLAST

    Proteomic databases

    MaxQBiQ80YQ2.
    PaxDbiQ80YQ2.
    PRIDEiQ80YQ2.

    PTM databases

    PhosphoSiteiQ80YQ2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ80YQ2.
    BgeeiQ80YQ2.
    CleanExiMM_MED23.
    GenevestigatoriQ80YQ2.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CEBPB, CTNNB1, and GLI3 By similarity. Interacts with CDK8 and ELK1. Interacts with the adenovirus E1A protein.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi213916. 2 interactions.
    DIPiDIP-59234N.
    IntActiQ80YQ2. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80YQ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 23 family.Curated

    Phylogenomic databases

    eggNOGiNOG305557.
    HOGENOMiHOG000043810.
    HOVERGENiHBG051122.
    KOiK15166.
    PhylomeDBiQ80YQ2.

    Family and domain databases

    InterProiIPR021629. Mediator_Med23.
    [Graphical view]
    PfamiPF11573. Med23. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q80YQ2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWSG     50
    LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVETG LLPPRMVCES 100
    LINSDSLEWE RTQLWALTFK LVRKIIGGVD YKGVRDLLKA ILEKILTIPN 150
    TVSSAVVQQL LAAREVIAYI LERNACLLPA YFAVTEIRKL YPEGKLPHWL 200
    LGNLVSDFVD TFRPTARINS ICGRCSLLPV VNNSGAICNS WKLDPATLRF 250
    PLKGLLPYDK DLFEPQTALL RYVLEQPYSR DMVCNMLGLN KQHKQRCPVL 300
    EDQLVDLVVY AMERSETEEK FDDGGTSQLL WQHLSSQLIF FVLFQFASFP 350
    HMVLSLHQKL AGRGLIKGRD HLMWVLLQFI SGSIQKNALA DFLPVMKLFD 400
    LLYPEKECIP VPDINKPQST HAFAMTCIWI HLNRKAQNGD STLQIPIPHS 450
    LKLHHEFLQQ SLRNKSLQMN DYKIALLCNA YSTNSECFTL PMGALVETIY 500
    GNGIMRVPLP GTSCLASASV TPLPMNLLDS LTVHAKMSLI HSIATRVIKL 550
    AHTKSSVALA PALVETYSRL LVYMEIESLG IKGFISQLLP TVFKSHAWGI 600
    LHTLLEMFSH RMHHIQPHYR VQLLSHLHTL AAVAQTNQNQ LHLCVESTAL 650
    RLITALGSSE VQPQFTRFLN DPKTVLSAES EELNRALILT LARATHVTDF 700
    FTGSDSIQGT WCKDILQTIM NFTPHNWASH TLSCFPAPLQ AFFKQNNVPQ 750
    ESRFNLKKNV EEEYRKWKSM TDENEIITQF SVQGFPPLFL CLLWKMLLET 800
    DHISQIGYKV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL 850
    NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLLL LKPNDFRNRV 900
    SDFVKENSPE HWLQSDWHTK HMSYHKKYPE KLYFEGLAEQ VDPPVPIQSP 950
    YLPIYFGNVC LRFLPVFDIV IHRFLELLPV SKSLETLLDH LGGLYKFHDR 1000
    PVTYLYNTLH YYEMCLRNRD HLKRKLVHAI IGSLKDNRPQ GWCLSDTYLK 1050
    HAMNAREDNP WVPEDSYYCK LIGRLVDTMA GKSPGPFPNC DWRFNEFPNP 1100
    AAHALHVTCV ELMALAVPGK DVGNALLNVV LKSQPLVPRE NITAWMNAIG 1150
    LIITALPEPY WIVLHDRIVN VISSSSLTSE TEWVGYPFRL FDFTACHQSY 1200
    SEMSCSYTLA LAHAVWHHSS IGQLSLIPKF LTEALLPVVK TEFQLLYVYH 1250
    LVGPFLQRFQ QERTRCMIEI GVAFYDMLLN VDQCSTHLNY MDPICDFLYH 1300
    MKYMFTGDSV KEQVEKIICN LKPALKLRLR FITHISKMEP AVPPQALNSG 1350
    SPAPQSNQVP ASLPVTQ 1367
    Length:1,367
    Mass (Da):156,087
    Last modified:October 2, 2007 - v2
    Checksum:iFD7764FFB2A52B1A
    GO
    Isoform 2 (identifier: Q80YQ2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         291-291: K → KQTLNIA

    Show »
    Length:1,373
    Mass (Da):156,728
    Checksum:i40B960CADA0749B1
    GO
    Isoform 3 (identifier: Q80YQ2-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         54-55: ES → IV
         56-1367: Missing.

    Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:55
    Mass (Da):6,343
    Checksum:i54EF12934E0622AA
    GO

    Sequence cautioni

    The sequence AAM28897.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence BAB29019.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence BAC98122.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 771S → F in AAH50916. (PubMed:15489334)Curated
    Sequence conflicti219 – 2191N → Y in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti358 – 3581Q → H in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti364 – 3641G → R in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti385 – 3851Q → H in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti809 – 8091K → N in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti812 – 8132ER → DK in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti840 – 8401G → S in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti844 – 8441N → G in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti1062 – 10654VPED → GSRKN in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti1091 – 10911D → E in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti1282 – 12821D → N in AAM28897. (PubMed:11934987)Curated
    Sequence conflicti1315 – 13151E → K in AAM28897. (PubMed:11934987)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei54 – 552ES → IV in isoform 3. 1 PublicationVSP_041583
    Alternative sequencei56 – 13671312Missing in isoform 3. 1 PublicationVSP_041584Add
    BLAST
    Alternative sequencei291 – 2911K → KQTLNIA in isoform 2. 1 PublicationVSP_028382

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF507918 mRNA. Translation: AAM28897.1. Different initiation.
    AK129312 mRNA. Translation: BAC98122.1. Different initiation.
    AK013854 mRNA. Translation: BAB29019.1. Different initiation.
    AK028545 mRNA. Translation: BAC26001.1. Sequence problems.
    BC005508 mRNA. Translation: AAH05508.2.
    BC050916 mRNA. Translation: AAH50916.1.
    RefSeqiNP_001159888.1. NM_001166416.1.
    NP_081623.3. NM_027347.3.
    UniGeneiMm.28020.

    Genome annotation databases

    GeneIDi70208.
    KEGGimmu:70208.
    UCSCiuc011xbu.1. mouse. [Q80YQ2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF507918 mRNA. Translation: AAM28897.1 . Different initiation.
    AK129312 mRNA. Translation: BAC98122.1 . Different initiation.
    AK013854 mRNA. Translation: BAB29019.1 . Different initiation.
    AK028545 mRNA. Translation: BAC26001.1 . Sequence problems.
    BC005508 mRNA. Translation: AAH05508.2 .
    BC050916 mRNA. Translation: AAH50916.1 .
    RefSeqi NP_001159888.1. NM_001166416.1.
    NP_081623.3. NM_027347.3.
    UniGenei Mm.28020.

    3D structure databases

    ProteinModelPortali Q80YQ2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 213916. 2 interactions.
    DIPi DIP-59234N.
    IntActi Q80YQ2. 4 interactions.

    PTM databases

    PhosphoSitei Q80YQ2.

    Proteomic databases

    MaxQBi Q80YQ2.
    PaxDbi Q80YQ2.
    PRIDEi Q80YQ2.

    Protocols and materials databases

    DNASUi 70208.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 70208.
    KEGGi mmu:70208.
    UCSCi uc011xbu.1. mouse. [Q80YQ2-1 ]

    Organism-specific databases

    CTDi 9439.
    MGIi MGI:1917458. Med23.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG305557.
    HOGENOMi HOG000043810.
    HOVERGENi HBG051122.
    KOi K15166.
    PhylomeDBi Q80YQ2.

    Enzyme and pathway databases

    Reactomei REACT_198602. PPARA activates gene expression.
    REACT_206529. Generic Transcription Pathway.
    REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Miscellaneous databases

    NextBioi 331172.
    PROi Q80YQ2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q80YQ2.
    Bgeei Q80YQ2.
    CleanExi MM_MED23.
    Genevestigatori Q80YQ2.

    Family and domain databases

    InterProi IPR021629. Mediator_Med23.
    [Graphical view ]
    Pfami PF11573. Med23. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit."
      Stevens J.L., Cantin G.T., Wang G., Shevchenko A., Shevchenko A., Berk A.J.
      Science 296:755-758(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH CDK8; E1A AND ELK1.
      Strain: C3H.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Embryonic tail.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1114-1367 (ISOFORMS 1/2).
      Strain: C57BL/6J.
      Tissue: Embryonic head and Skin.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 673-1367 (ISOFORMS 1/2).
      Strain: C57BL/6.
      Tissue: Brain and Mammary tumor.
    5. "Requirement of Sur2 for efficient replication of mouse adenovirus type 1."
      Fang L., Stevens J.L., Berk A.J., Spindler K.R.
      J. Virol. 78:12888-12900(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH E1A.
    6. "Ras induces mediator complex exchange on C/EBP beta."
      Mo X., Kowenz-Leutz E., Xu H., Leutz A.
      Mol. Cell 13:241-250(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Thyroid hormone-induced juxtaposition of regulatory elements/factors and chromatin remodeling of Crabp1 dependent on MED1/TRAP220."
      Park S.W., Li G., Lin Y.-P., Barrero M.J., Ge K., Roeder R.G., Wei L.-N.
      Mol. Cell 19:643-653(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH PROMOTER REGIONS.
    8. "Mediator requirement for both recruitment and postrecruitment steps in transcription initiation."
      Wang G., Balamotis M.A., Stevens J.L., Yamaguchi Y., Handa H., Berk A.J.
      Mol. Cell 17:683-694(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMED23_MOUSE
    AccessioniPrimary (citable) accession number: Q80YQ2
    Secondary accession number(s): Q6ZPV7
    , Q8CEC3, Q8K587, Q9CXY8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3