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Protein

Mediator of RNA polymerase II transcription subunit 23

Gene

Med23

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). Also required for transcriptional activation subsequent to the assembly of the preinitiation complex. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.By similarity4 Publications

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: MGI
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_198602. PPARA activates gene expression.
REACT_206529. Generic Transcription Pathway.
REACT_252217. Transcriptional regulation of white adipocyte differentiation.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 23
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 3
Short name:
CRSP complex subunit 3
Mediator complex subunit 23
Protein sur-2 homolog
Short name:
mSur-2
Gene namesi
Name:Med23
Synonyms:Crsp3, Kiaa1216, Sur2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1917458. Med23.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. mediator complex Source: MGI
  2. nucleoplasm Source: Reactome
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13671367Mediator of RNA polymerase II transcription subunit 23PRO_0000305931Add
BLAST

Proteomic databases

MaxQBiQ80YQ2.
PaxDbiQ80YQ2.
PRIDEiQ80YQ2.

PTM databases

PhosphoSiteiQ80YQ2.

Expressioni

Gene expression databases

BgeeiQ80YQ2.
CleanExiMM_MED23.
ExpressionAtlasiQ80YQ2. baseline and differential.
GenevestigatoriQ80YQ2.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CEBPB, CTNNB1, and GLI3 (By similarity). Interacts with CDK8 and ELK1. Interacts with the adenovirus E1A protein.By similarity2 Publications

Protein-protein interaction databases

BioGridi213916. 2 interactions.
DIPiDIP-59234N.
IntActiQ80YQ2. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ80YQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 23 family.Curated

Phylogenomic databases

eggNOGiNOG305557.
HOGENOMiHOG000043810.
HOVERGENiHBG051122.
InParanoidiQ80YQ2.
KOiK15166.
PhylomeDBiQ80YQ2.

Family and domain databases

InterProiIPR021629. Mediator_Med23.
[Graphical view]
PfamiPF11573. Med23. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80YQ2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWSG
60 70 80 90 100
LSQESHEQCV QWIVKFIHGQ HSPKRISFLY DCLAMAVETG LLPPRMVCES
110 120 130 140 150
LINSDSLEWE RTQLWALTFK LVRKIIGGVD YKGVRDLLKA ILEKILTIPN
160 170 180 190 200
TVSSAVVQQL LAAREVIAYI LERNACLLPA YFAVTEIRKL YPEGKLPHWL
210 220 230 240 250
LGNLVSDFVD TFRPTARINS ICGRCSLLPV VNNSGAICNS WKLDPATLRF
260 270 280 290 300
PLKGLLPYDK DLFEPQTALL RYVLEQPYSR DMVCNMLGLN KQHKQRCPVL
310 320 330 340 350
EDQLVDLVVY AMERSETEEK FDDGGTSQLL WQHLSSQLIF FVLFQFASFP
360 370 380 390 400
HMVLSLHQKL AGRGLIKGRD HLMWVLLQFI SGSIQKNALA DFLPVMKLFD
410 420 430 440 450
LLYPEKECIP VPDINKPQST HAFAMTCIWI HLNRKAQNGD STLQIPIPHS
460 470 480 490 500
LKLHHEFLQQ SLRNKSLQMN DYKIALLCNA YSTNSECFTL PMGALVETIY
510 520 530 540 550
GNGIMRVPLP GTSCLASASV TPLPMNLLDS LTVHAKMSLI HSIATRVIKL
560 570 580 590 600
AHTKSSVALA PALVETYSRL LVYMEIESLG IKGFISQLLP TVFKSHAWGI
610 620 630 640 650
LHTLLEMFSH RMHHIQPHYR VQLLSHLHTL AAVAQTNQNQ LHLCVESTAL
660 670 680 690 700
RLITALGSSE VQPQFTRFLN DPKTVLSAES EELNRALILT LARATHVTDF
710 720 730 740 750
FTGSDSIQGT WCKDILQTIM NFTPHNWASH TLSCFPAPLQ AFFKQNNVPQ
760 770 780 790 800
ESRFNLKKNV EEEYRKWKSM TDENEIITQF SVQGFPPLFL CLLWKMLLET
810 820 830 840 850
DHISQIGYKV LERIGARALV AHVRTFADFL VYEFSTSAGG QQLNKCIEIL
860 870 880 890 900
NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLLL LKPNDFRNRV
910 920 930 940 950
SDFVKENSPE HWLQSDWHTK HMSYHKKYPE KLYFEGLAEQ VDPPVPIQSP
960 970 980 990 1000
YLPIYFGNVC LRFLPVFDIV IHRFLELLPV SKSLETLLDH LGGLYKFHDR
1010 1020 1030 1040 1050
PVTYLYNTLH YYEMCLRNRD HLKRKLVHAI IGSLKDNRPQ GWCLSDTYLK
1060 1070 1080 1090 1100
HAMNAREDNP WVPEDSYYCK LIGRLVDTMA GKSPGPFPNC DWRFNEFPNP
1110 1120 1130 1140 1150
AAHALHVTCV ELMALAVPGK DVGNALLNVV LKSQPLVPRE NITAWMNAIG
1160 1170 1180 1190 1200
LIITALPEPY WIVLHDRIVN VISSSSLTSE TEWVGYPFRL FDFTACHQSY
1210 1220 1230 1240 1250
SEMSCSYTLA LAHAVWHHSS IGQLSLIPKF LTEALLPVVK TEFQLLYVYH
1260 1270 1280 1290 1300
LVGPFLQRFQ QERTRCMIEI GVAFYDMLLN VDQCSTHLNY MDPICDFLYH
1310 1320 1330 1340 1350
MKYMFTGDSV KEQVEKIICN LKPALKLRLR FITHISKMEP AVPPQALNSG
1360
SPAPQSNQVP ASLPVTQ
Length:1,367
Mass (Da):156,087
Last modified:October 2, 2007 - v2
Checksum:iFD7764FFB2A52B1A
GO
Isoform 2 (identifier: Q80YQ2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     291-291: K → KQTLNIA

Show »
Length:1,373
Mass (Da):156,728
Checksum:i40B960CADA0749B1
GO
Isoform 3 (identifier: Q80YQ2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-55: ES → IV
     56-1367: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:55
Mass (Da):6,343
Checksum:i54EF12934E0622AA
GO

Sequence cautioni

The sequence AAM28897.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB29019.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC98122.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771S → F in AAH50916 (PubMed:15489334).Curated
Sequence conflicti219 – 2191N → Y in AAM28897 (PubMed:11934987).Curated
Sequence conflicti358 – 3581Q → H in AAM28897 (PubMed:11934987).Curated
Sequence conflicti364 – 3641G → R in AAM28897 (PubMed:11934987).Curated
Sequence conflicti385 – 3851Q → H in AAM28897 (PubMed:11934987).Curated
Sequence conflicti809 – 8091K → N in AAM28897 (PubMed:11934987).Curated
Sequence conflicti812 – 8132ER → DK in AAM28897 (PubMed:11934987).Curated
Sequence conflicti840 – 8401G → S in AAM28897 (PubMed:11934987).Curated
Sequence conflicti844 – 8441N → G in AAM28897 (PubMed:11934987).Curated
Sequence conflicti1062 – 10654VPED → GSRKN in AAM28897 (PubMed:11934987).Curated
Sequence conflicti1091 – 10911D → E in AAM28897 (PubMed:11934987).Curated
Sequence conflicti1282 – 12821D → N in AAM28897 (PubMed:11934987).Curated
Sequence conflicti1315 – 13151E → K in AAM28897 (PubMed:11934987).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei54 – 552ES → IV in isoform 3. 1 PublicationVSP_041583
Alternative sequencei56 – 13671312Missing in isoform 3. 1 PublicationVSP_041584Add
BLAST
Alternative sequencei291 – 2911K → KQTLNIA in isoform 2. 1 PublicationVSP_028382

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF507918 mRNA. Translation: AAM28897.1. Different initiation.
AK129312 mRNA. Translation: BAC98122.1. Different initiation.
AK013854 mRNA. Translation: BAB29019.1. Different initiation.
AK028545 mRNA. Translation: BAC26001.1. Sequence problems.
BC005508 mRNA. Translation: AAH05508.2.
BC050916 mRNA. Translation: AAH50916.1.
RefSeqiNP_001159888.1. NM_001166416.1.
NP_081623.3. NM_027347.3.
UniGeneiMm.28020.

Genome annotation databases

GeneIDi70208.
KEGGimmu:70208.
UCSCiuc011xbu.1. mouse. [Q80YQ2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF507918 mRNA. Translation: AAM28897.1. Different initiation.
AK129312 mRNA. Translation: BAC98122.1. Different initiation.
AK013854 mRNA. Translation: BAB29019.1. Different initiation.
AK028545 mRNA. Translation: BAC26001.1. Sequence problems.
BC005508 mRNA. Translation: AAH05508.2.
BC050916 mRNA. Translation: AAH50916.1.
RefSeqiNP_001159888.1. NM_001166416.1.
NP_081623.3. NM_027347.3.
UniGeneiMm.28020.

3D structure databases

ProteinModelPortaliQ80YQ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213916. 2 interactions.
DIPiDIP-59234N.
IntActiQ80YQ2. 4 interactions.

PTM databases

PhosphoSiteiQ80YQ2.

Proteomic databases

MaxQBiQ80YQ2.
PaxDbiQ80YQ2.
PRIDEiQ80YQ2.

Protocols and materials databases

DNASUi70208.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi70208.
KEGGimmu:70208.
UCSCiuc011xbu.1. mouse. [Q80YQ2-1]

Organism-specific databases

CTDi9439.
MGIiMGI:1917458. Med23.
RougeiSearch...

Phylogenomic databases

eggNOGiNOG305557.
HOGENOMiHOG000043810.
HOVERGENiHBG051122.
InParanoidiQ80YQ2.
KOiK15166.
PhylomeDBiQ80YQ2.

Enzyme and pathway databases

ReactomeiREACT_198602. PPARA activates gene expression.
REACT_206529. Generic Transcription Pathway.
REACT_252217. Transcriptional regulation of white adipocyte differentiation.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSiMed23. mouse.
NextBioi331172.
PROiQ80YQ2.
SOURCEiSearch...

Gene expression databases

BgeeiQ80YQ2.
CleanExiMM_MED23.
ExpressionAtlasiQ80YQ2. baseline and differential.
GenevestigatoriQ80YQ2.

Family and domain databases

InterProiIPR021629. Mediator_Med23.
[Graphical view]
PfamiPF11573. Med23. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit."
    Stevens J.L., Cantin G.T., Wang G., Shevchenko A., Shevchenko A., Berk A.J.
    Science 296:755-758(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH CDK8; E1A AND ELK1.
    Strain: C3H.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Embryonic tail.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1114-1367 (ISOFORMS 1/2).
    Strain: C57BL/6J.
    Tissue: Embryonic head and Skin.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 673-1367 (ISOFORMS 1/2).
    Strain: C57BL/6.
    Tissue: Brain and Mammary tumor.
  5. "Requirement of Sur2 for efficient replication of mouse adenovirus type 1."
    Fang L., Stevens J.L., Berk A.J., Spindler K.R.
    J. Virol. 78:12888-12900(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH E1A.
  6. "Ras induces mediator complex exchange on C/EBP beta."
    Mo X., Kowenz-Leutz E., Xu H., Leutz A.
    Mol. Cell 13:241-250(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Thyroid hormone-induced juxtaposition of regulatory elements/factors and chromatin remodeling of Crabp1 dependent on MED1/TRAP220."
    Park S.W., Li G., Lin Y.-P., Barrero M.J., Ge K., Roeder R.G., Wei L.-N.
    Mol. Cell 19:643-653(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSOCIATION WITH PROMOTER REGIONS.
  8. "Mediator requirement for both recruitment and postrecruitment steps in transcription initiation."
    Wang G., Balamotis M.A., Stevens J.L., Yamaguchi Y., Handa H., Berk A.J.
    Mol. Cell 17:683-694(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMED23_MOUSE
AccessioniPrimary (citable) accession number: Q80YQ2
Secondary accession number(s): Q6ZPV7
, Q8CEC3, Q8K587, Q9CXY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: February 4, 2015
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.