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Q80YP0

- CDK3_MOUSE

UniProt

Q80YP0 - CDK3_MOUSE

Protein

Cyclin-dependent kinase 3

Gene

Cdk3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 3 (EC:2.7.11.22)
    Alternative name(s):
    Cell division protein kinase 3
    Gene namesi
    Name:Cdk3
    Synonyms:Cdk3-ps, Cdkn3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1916931. Cdk3-ps.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 303303Cyclin-dependent kinase 3PRO_0000085777Add
    BLAST

    Proteomic databases

    PaxDbiQ80YP0.
    PRIDEiQ80YP0.

    PTM databases

    PhosphoSiteiQ80YP0.

    Expressioni

    Developmental stagei

    Expressed in heart, lung, nasal cavity, roof-plate of the fourth ventricle, skin, cartilage primordium of the basisphenoid and basioccipital bone at 12.5 dpc.1 Publication

    Gene expression databases

    BgeeiQ80YP0.
    CleanExiMM_CDK3.
    GenevestigatoriQ80YP0.

    Interactioni

    Subunit structurei

    Interacts with CABLES1 and ATF1. Binding to CCNC/cyclin-C promotes RB1 phosphorylation By similarity. Binds to CABLES2.By similarity1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ80YP0.
    SMRiQ80YP0. Positions 1-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 285282Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG014652.
    InParanoidiQ80YP0.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q80YP0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDVFQKVEKI GEGTYGVVYK ARNKVTGQLV ALKKIRLDLE AEGVPSTAVR    50
    EISLLKELKH PNIIKLLDVV HREKKLYMVF EFLTQDLKRH MDSSPTSELP 100
    LPVVKSYLAQ LLEGVSFCHS HRVIHRDLKP QNLLLDGLGA IKLADFGLAR 150
    AFGVPLRTYT HEVVTLWYRA PEILLGSKFY STAVDISIGC IFAEMVTGKA 200
    LFPGDSEIDQ LFRIFRTLGT PSEATWPGVS QMPDYQSSFP KWSRKGLEEI 250
    VPSLGPEGKD LLLRLLQYDP SQRISAKTAL AHPYFSPGHS LAPQQCTAGR 300
    SSR 303
    Length:303
    Mass (Da):33,888
    Last modified:March 20, 2007 - v2
    Checksum:iD83EAAA8C133BC88
    GO
    Isoform 2 (identifier: Q80YP0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-194: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:109
    Mass (Da):12,034
    Checksum:i5FCE672BA6403FE4
    GO

    Polymorphismi

    A point mutation converts Trp-187 to a premature termination codon, resulting in a truncated form that has no catalytic activity. This truncation occurs near the T loop, which is involved in activation by CDK-activating kinase and deletes motif XI known to be required for kinase function. Consequently, the truncated protein generates a null allele. This mutation is found in laboratory strains but not in wild-mice species such as M.spretus and M.musculus castaneus.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti187 – 303117Missing in truncated, inactive form.
    Add
    BLAST

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 194194Missing in isoform 2. 1 PublicationVSP_023572Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF327523 Genomic DNA. Translation: AAK51354.1.
    AK009918 mRNA. Translation: BAB26584.1.
    AK166387 mRNA. Translation: BAE38746.1.
    AL669925 Genomic DNA. No translation available.
    UniGeneiMm.33677.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF327523 Genomic DNA. Translation: AAK51354.1 .
    AK009918 mRNA. Translation: BAB26584.1 .
    AK166387 mRNA. Translation: BAE38746.1 .
    AL669925 Genomic DNA. No translation available.
    UniGenei Mm.33677.

    3D structure databases

    ProteinModelPortali Q80YP0.
    SMRi Q80YP0. Positions 1-285.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q80YP0.

    Proteomic databases

    PaxDbi Q80YP0.
    PRIDEi Q80YP0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:1916931. Cdk3-ps.

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG014652.
    InParanoidi Q80YP0.

    Miscellaneous databases

    PROi Q80YP0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q80YP0.
    CleanExi MM_CDK3.
    Genevestigatori Q80YP0.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A premature-termination mutation in the Mus musculus cyclin-dependent kinase 3 gene."
      Ye X., Zhu C., Harper J.W.
      Proc. Natl. Acad. Sci. U.S.A. 98:1682-1686(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, POLYMORPHISM.
      Strain: 129/Sv and C57BL/6J.
      Tissue: Tongue.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Mammary gland.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Cited for: FUNCTION AS CABLES1 KINASE.
    5. "Ik3-2, a relative to ik3-1/cables, is associated with cdk3, cdk5, and c-abl."
      Sato H., Nishimoto I., Matsuoka M.
      Biochim. Biophys. Acta 1574:157-163(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CABLES2.

    Entry informationi

    Entry nameiCDK3_MOUSE
    AccessioniPrimary (citable) accession number: Q80YP0
    Secondary accession number(s): Q3TLP6, Q9D6V6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3