Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q80YP0 (CDK3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 3

EC=2.7.11.22
Alternative name(s):
Cell division protein kinase 3
Gene names
Name:Cdk3
Synonyms:Cdk3-ps, Cdkn3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner. Ref.4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with CABLES1 and ATF1. Binding to CCNC/cyclin-C promotes RB1 phosphorylation By similarity. Binds to CABLES2. Ref.5

Developmental stage

Expressed in heart, lung, nasal cavity, roof-plate of the fourth ventricle, skin, cartilage primordium of the basisphenoid and basioccipital bone at 12.5 dpc. Ref.1

Polymorphism

A point mutation converts Trp-187 to a premature termination codon, resulting in a truncated form that has no catalytic activity. This truncation occurs near the T loop, which is involved in activation by CDK-activating kinase and deletes motif XI known to be required for kinase function. Consequently, the truncated protein generates a null allele. This mutation is found in laboratory strains but not in wild-mice species such as M.spretus and M.musculus castaneus.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Caution

Defined as a polymorphic pseudogene by MGI.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q80YP0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q80YP0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-194: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303Cyclin-dependent kinase 3
PRO_0000085777

Regions

Domain4 – 285282Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1271Proton acceptor By similarity
Binding site331ATP By similarity

Natural variations

Alternative sequence1 – 194194Missing in isoform 2.
VSP_023572
Natural variant187 – 303117Missing in truncated, inactive form.

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 20, 2007. Version 2.
Checksum: D83EAAA8C133BC88

FASTA30333,888
        10         20         30         40         50         60 
MDVFQKVEKI GEGTYGVVYK ARNKVTGQLV ALKKIRLDLE AEGVPSTAVR EISLLKELKH 

        70         80         90        100        110        120 
PNIIKLLDVV HREKKLYMVF EFLTQDLKRH MDSSPTSELP LPVVKSYLAQ LLEGVSFCHS 

       130        140        150        160        170        180 
HRVIHRDLKP QNLLLDGLGA IKLADFGLAR AFGVPLRTYT HEVVTLWYRA PEILLGSKFY 

       190        200        210        220        230        240 
STAVDISIGC IFAEMVTGKA LFPGDSEIDQ LFRIFRTLGT PSEATWPGVS QMPDYQSSFP 

       250        260        270        280        290        300 
KWSRKGLEEI VPSLGPEGKD LLLRLLQYDP SQRISAKTAL AHPYFSPGHS LAPQQCTAGR 


SSR 

« Hide

Isoform 2 [UniParc].

Checksum: 5FCE672BA6403FE4
Show »

FASTA10912,034

References

« Hide 'large scale' references
[1]"A premature-termination mutation in the Mus musculus cyclin-dependent kinase 3 gene."
Ye X., Zhu C., Harper J.W.
Proc. Natl. Acad. Sci. U.S.A. 98:1682-1686(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, POLYMORPHISM.
Strain: 129/Sv and C57BL/6J.
Tissue: Tongue.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Mammary gland.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"ik3-1/Cables is a substrate for cyclin-dependent kinase 3 (cdk 3)."
Yamochi T., Semba K., Tsuji K., Mizumoto K., Sato H., Matsuura Y., Nishimoto I., Matsuoka M.
Eur. J. Biochem. 268:6076-6082(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS CABLES1 KINASE.
[5]"Ik3-2, a relative to ik3-1/cables, is associated with cdk3, cdk5, and c-abl."
Sato H., Nishimoto I., Matsuoka M.
Biochim. Biophys. Acta 1574:157-163(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CABLES2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF327523 Genomic DNA. Translation: AAK51354.1.
AK009918 mRNA. Translation: BAB26584.1.
AK166387 mRNA. Translation: BAE38746.1.
AL669925 Genomic DNA. No translation available.
UniGeneMm.33677.

3D structure databases

ProteinModelPortalQ80YP0.
SMRQ80YP0. Positions 1-285.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ80YP0.

Proteomic databases

PaxDbQ80YP0.
PRIDEQ80YP0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:1916931. Cdk3-ps.

Phylogenomic databases

eggNOGCOG0515.
HOVERGENHBG014652.
InParanoidQ80YP0.

Gene expression databases

BgeeQ80YP0.
CleanExMM_CDK3.
GenevestigatorQ80YP0.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ80YP0.
SOURCESearch...

Entry information

Entry nameCDK3_MOUSE
AccessionPrimary (citable) accession number: Q80YP0
Secondary accession number(s): Q3TLP6, Q9D6V6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: March 20, 2007
Last modified: February 19, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot