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Protein

Rho GTPase-activating protein 33

Gene

Arhgap33

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in several stages of intracellular trafficking (By similarity). Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.By similarity

GO - Molecular functioni

  1. GTPase activator activity Source: MGI
  2. phosphatidylinositol binding Source: InterPro

GO - Biological processi

  1. positive regulation of GTPase activity Source: GOC
  2. protein transport Source: UniProtKB-KW
  3. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_297947. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 33
Alternative name(s):
Rho-type GTPase-activating protein 33
Sorting nexin-26
Tc10/CDC42 GTPase-activating protein
Gene namesi
Name:Arhgap33
Synonyms:Snx26, Tcgap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2673998. Arhgap33.

Subcellular locationi

  1. Cell membrane 1 Publication

  2. Note: Translocates to the plasma membrane in response to insulin in adipocytes.

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13051305Rho GTPase-activating protein 33PRO_0000056722Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei594 – 5941Phosphoserine1 Publication
Modified residuei660 – 6601PhosphoserineBy similarity
Modified residuei1188 – 11881Phosphotyrosine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ80YF9.
PaxDbiQ80YF9.
PRIDEiQ80YF9.

PTM databases

PhosphoSiteiQ80YF9.

Expressioni

Tissue specificityi

Highly expressed in brain and testis. Also expressed in white adipose tissue (WAT) and muscle at a low level.1 Publication

Inductioni

Dramatically induced during adipocyte differentiation.1 Publication

Gene expression databases

BgeeiQ80YF9.
CleanExiMM_SNX26.
GenevestigatoriQ80YF9.

Interactioni

Subunit structurei

Specifically interacts with CDC42 and RHOQ/TC10 through its Rho-GAP domain. Interacts with NEK6 (By similarity).By similarity

Protein-protein interaction databases

BioGridi231366. 4 interactions.
IntActiQ80YF9. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ80YF9.
SMRiQ80YF9. Positions 221-272, 335-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini83 – 192110PX; atypicalAdd
BLAST
Domaini210 – 27263SH3PROSITE-ProRule annotationAdd
BLAST
Domaini339 – 534196Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi150 – 1534Poly-Pro
Compositional biasi701 – 72121Poly-SerAdd
BLAST
Compositional biasi909 – 9135Poly-Pro
Compositional biasi1158 – 11614Poly-Pro
Compositional biasi1202 – 12076Poly-Ser
Compositional biasi1284 – 12918Poly-Pro

Domaini

The N-terminal PX domain interacts specifically with phosphatidylinositol 4,5-bisphosphate.

Sequence similaritiesi

Belongs to the PX domain-containing GAP family.Curated
Contains 1 PX (phox homology) domain.Curated
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG311367.
GeneTreeiENSGT00720000108475.
HOVERGENiHBG079688.
InParanoidiQ80YF9.
KOiK17933.
OMAiCFPPDHL.
OrthoDBiEOG7WMCHV.
PhylomeDBiQ80YF9.
TreeFamiTF351451.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR001683. Phox.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80YF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQAQKQSDP ILPWGASWAG RGQTLRARST DSLDGPGEGS VQPVPTTGGP
60 70 80 90 100
GTKGKPGKRL SAPRGPFPRL ADCAHFHYEN VDFGHIQLLL SPEREGPSLS
110 120 130 140 150
GENELVFGVQ VTCQGRSWPV LRSYDDFRSL DAHLHRCIFD RRFSCLPELP
160 170 180 190 200
PPPEGTRAAQ MLVPLLLQYL ETLSGLVDSN LNCGPVLTWM ELDNHGRRLL
210 220 230 240 250
LSEEASLNIP AVAAAHVVKR YTAQAPDELS FEVGDIVSVI DMPPTEDRSW
260 270 280 290 300
WRGKRGFQVG FFPSECVELF TERPGPGLKA DADSPLCGIP APQGISSLTS
310 320 330 340 350
AVPRPRGKLA GLLRTFMRSR PSRQRLRQRG ILRQRVFGCD LGEHLSNSGQ
360 370 380 390 400
DVPQVLRCCS EFIEAHGVVD GIYRLSGVSS NIQRLRHEFD SERIPELSGP
410 420 430 440 450
AFLQDIHSVS SLCKLYFREL PNPLLTYQLY GKFSEAMSVP GEEERLVRVH
460 470 480 490 500
DVIQQLPPPH YRTLEYLLRH LARMARHSAN TSMHARNLAI VWAPNLLRSM
510 520 530 540 550
ELESVGLGGA AAFREVRVQS VVVEFLLTHV EVLFSDTFTS AGLDPAGRCL
560 570 580 590 600
LPRPKSLAGS SPSTRLLTLE EAQARTQGRL GTPTEPTTPK TPASPVERRK
610 620 630 640 650
RERAEKQRKP GGSSWKTFFA LGRGPSIPRK KPLPWLGGSR APPQPSGSRP
660 670 680 690 700
DTVTLRSAKS EESLSSQASG AGLQRLHRLR RPHSSSDAFP VGPAPAGSCE
710 720 730 740 750
SLSSSSSSSS SSSSSSSSES SAGGLGPLSG SPSHRTSAWL DDGDDLDFSP
760 770 780 790 800
PRCLEGLRGL DFDPLTFRCS SPTPGDPAPP ASPAPPASAS AFPPRATPQA
810 820 830 840 850
LSPHGPTKPA SPTALDISEP LAVSVPPAVL ELLGAGGTPA SATPTPALSP
860 870 880 890 900
HLIPLLLRGA EAQLSDTCQQ EISSKLAPTR GAPGQQSPGG MDSPLLPPPL
910 920 930 940 950
PLLRPGGAPP PPPKNPARLM ALALAERAQQ VAEQQSQQEQ GGTPPAPHSP
960 970 980 990 1000
FRRSLSLEVG GEPVGTSGSG IHPPSLAHPG AWAPGPPPYL PRQQSDGSLV
1010 1020 1030 1040 1050
RSQRPLGTSR RSPRGPSQVS AHLRASGAYR DAPEMAAQSP CSVPSQGSNP
1060 1070 1080 1090 1100
SFFSTPRECL PPFLGVPKQG LYSLGPPSFP PSSPAPVWRN SLGAPSALDR
1110 1120 1130 1140 1150
GENLYYEIGV GEGTSYSGPS RSWSPFRSMP PDRHNASYGM LGQSPPLHRS
1160 1170 1180 1190 1200
PDFLLSYPPP PSCFPPEHLT HSVSQRLARR PTRPEPLYVN LALGPRGPSP
1210 1220 1230 1240 1250
ASSSSSSPPA HPRSRSDPGP PVPRLPQKQR APWGPHTPHR VPGPWGSPEP
1260 1270 1280 1290 1300
FLLYRPAPPS YGRGGEVRGS LYRNGGHRGE GAGPPPPYPT PSWSLHSEGQ

TRSYC
Length:1,305
Mass (Da):139,801
Last modified:June 1, 2003 - v1
Checksum:iAA8723BDF4A3CBB6
GO

Sequence cautioni

The sequence AAH61471.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti357 – 3571R → C in AAH61471 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY217764 mRNA. Translation: AAO89073.1.
BC065086 mRNA. Translation: AAH65086.1.
BC065166 mRNA. Translation: AAH65166.1.
BC066047 mRNA. Translation: AAH66047.1.
BC061471 mRNA. Translation: AAH61471.1. Different initiation.
CCDSiCCDS21095.1.
RefSeqiNP_001276599.1. NM_001289670.1.
NP_001276611.1. NM_001289682.1.
NP_839983.1. NM_178252.3.
XP_006539951.1. XM_006539888.2.
UniGeneiMm.190704.

Genome annotation databases

EnsembliENSMUST00000044338; ENSMUSP00000038412; ENSMUSG00000036882.
GeneIDi233071.
KEGGimmu:233071.
UCSCiuc009geq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY217764 mRNA. Translation: AAO89073.1.
BC065086 mRNA. Translation: AAH65086.1.
BC065166 mRNA. Translation: AAH65166.1.
BC066047 mRNA. Translation: AAH66047.1.
BC061471 mRNA. Translation: AAH61471.1. Different initiation.
CCDSiCCDS21095.1.
RefSeqiNP_001276599.1. NM_001289670.1.
NP_001276611.1. NM_001289682.1.
NP_839983.1. NM_178252.3.
XP_006539951.1. XM_006539888.2.
UniGeneiMm.190704.

3D structure databases

ProteinModelPortaliQ80YF9.
SMRiQ80YF9. Positions 221-272, 335-535.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231366. 4 interactions.
IntActiQ80YF9. 1 interaction.

PTM databases

PhosphoSiteiQ80YF9.

Proteomic databases

MaxQBiQ80YF9.
PaxDbiQ80YF9.
PRIDEiQ80YF9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044338; ENSMUSP00000038412; ENSMUSG00000036882.
GeneIDi233071.
KEGGimmu:233071.
UCSCiuc009geq.1. mouse.

Organism-specific databases

CTDi115703.
MGIiMGI:2673998. Arhgap33.

Phylogenomic databases

eggNOGiNOG311367.
GeneTreeiENSGT00720000108475.
HOVERGENiHBG079688.
InParanoidiQ80YF9.
KOiK17933.
OMAiCFPPDHL.
OrthoDBiEOG7WMCHV.
PhylomeDBiQ80YF9.
TreeFamiTF351451.

Enzyme and pathway databases

ReactomeiREACT_297947. Rho GTPase cycle.

Miscellaneous databases

NextBioi381531.
PROiQ80YF9.
SOURCEiSearch...

Gene expression databases

BgeeiQ80YF9.
CleanExiMM_SNX26.
GenevestigatoriQ80YF9.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR001683. Phox.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TCGAP, a multidomain Rho GTPase-activating protein involved in insulin-stimulated glucose transport."
    Chiang S.-H., Hwang J., Legendre M., Zhang M., Kimura A., Saltiel A.R.
    EMBO J. 22:2679-2691(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH CDC42 AND RHOQ, POSSIBLE FUNCTION.
    Tissue: Adipocyte.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 220-1305.
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-594, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  4. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1188, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiRHG33_MOUSE
AccessioniPrimary (citable) accession number: Q80YF9
Secondary accession number(s): Q6P7W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2003
Last modified: April 29, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.