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Q80YF9 (RHG33_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho GTPase-activating protein 33
Alternative name(s):
Rho-type GTPase-activating protein 33
Sorting nexin-26
Tc10/CDC42 GTPase-activating protein
Gene names
Name:Arhgap33
Synonyms:Snx26, Tcgap
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1305 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in several stages of intracellular trafficking By similarity. Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport. Ref.1

Subunit structure

Specifically interacts with CDC42 and RHOQ/TC10 through its Rho-GAP domain. Interacts with NEK6 By similarity. Ref.1

Subcellular location

Cell membrane. Note: Translocates to the plasma membrane in response to insulin in adipocytes. Ref.1

Tissue specificity

Highly expressed in brain and testis. Also expressed in white adipose tissue (WAT) and muscle at a low level. Ref.1

Induction

Dramatically induced during adipocyte differentiation. Ref.1

Domain

The N-terminal PX domain interacts specifically with phosphatidylinositol 4,5-bisphosphate.

Sequence similarities

Belongs to the PX domain-containing GAP family.

Contains 1 PX (phox homology) domain.

Contains 1 Rho-GAP domain.

Contains 1 SH3 domain.

Sequence caution

The sequence AAH61471.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13051305Rho GTPase-activating protein 33
PRO_0000056722

Regions

Domain83 – 192110PX; atypical
Domain210 – 27263SH3
Domain339 – 534196Rho-GAP
Compositional bias150 – 1534Poly-Pro
Compositional bias701 – 72121Poly-Ser
Compositional bias909 – 9135Poly-Pro
Compositional bias1158 – 11614Poly-Pro
Compositional bias1202 – 12076Poly-Ser
Compositional bias1284 – 12918Poly-Pro

Amino acid modifications

Modified residue5941Phosphoserine Ref.3
Modified residue6601Phosphoserine By similarity
Modified residue11881Phosphotyrosine Ref.4

Experimental info

Sequence conflict3571R → C in AAH61471. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q80YF9 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: AA8723BDF4A3CBB6

FASTA1,305139,801
        10         20         30         40         50         60 
MLQAQKQSDP ILPWGASWAG RGQTLRARST DSLDGPGEGS VQPVPTTGGP GTKGKPGKRL 

        70         80         90        100        110        120 
SAPRGPFPRL ADCAHFHYEN VDFGHIQLLL SPEREGPSLS GENELVFGVQ VTCQGRSWPV 

       130        140        150        160        170        180 
LRSYDDFRSL DAHLHRCIFD RRFSCLPELP PPPEGTRAAQ MLVPLLLQYL ETLSGLVDSN 

       190        200        210        220        230        240 
LNCGPVLTWM ELDNHGRRLL LSEEASLNIP AVAAAHVVKR YTAQAPDELS FEVGDIVSVI 

       250        260        270        280        290        300 
DMPPTEDRSW WRGKRGFQVG FFPSECVELF TERPGPGLKA DADSPLCGIP APQGISSLTS 

       310        320        330        340        350        360 
AVPRPRGKLA GLLRTFMRSR PSRQRLRQRG ILRQRVFGCD LGEHLSNSGQ DVPQVLRCCS 

       370        380        390        400        410        420 
EFIEAHGVVD GIYRLSGVSS NIQRLRHEFD SERIPELSGP AFLQDIHSVS SLCKLYFREL 

       430        440        450        460        470        480 
PNPLLTYQLY GKFSEAMSVP GEEERLVRVH DVIQQLPPPH YRTLEYLLRH LARMARHSAN 

       490        500        510        520        530        540 
TSMHARNLAI VWAPNLLRSM ELESVGLGGA AAFREVRVQS VVVEFLLTHV EVLFSDTFTS 

       550        560        570        580        590        600 
AGLDPAGRCL LPRPKSLAGS SPSTRLLTLE EAQARTQGRL GTPTEPTTPK TPASPVERRK 

       610        620        630        640        650        660 
RERAEKQRKP GGSSWKTFFA LGRGPSIPRK KPLPWLGGSR APPQPSGSRP DTVTLRSAKS 

       670        680        690        700        710        720 
EESLSSQASG AGLQRLHRLR RPHSSSDAFP VGPAPAGSCE SLSSSSSSSS SSSSSSSSES 

       730        740        750        760        770        780 
SAGGLGPLSG SPSHRTSAWL DDGDDLDFSP PRCLEGLRGL DFDPLTFRCS SPTPGDPAPP 

       790        800        810        820        830        840 
ASPAPPASAS AFPPRATPQA LSPHGPTKPA SPTALDISEP LAVSVPPAVL ELLGAGGTPA 

       850        860        870        880        890        900 
SATPTPALSP HLIPLLLRGA EAQLSDTCQQ EISSKLAPTR GAPGQQSPGG MDSPLLPPPL 

       910        920        930        940        950        960 
PLLRPGGAPP PPPKNPARLM ALALAERAQQ VAEQQSQQEQ GGTPPAPHSP FRRSLSLEVG 

       970        980        990       1000       1010       1020 
GEPVGTSGSG IHPPSLAHPG AWAPGPPPYL PRQQSDGSLV RSQRPLGTSR RSPRGPSQVS 

      1030       1040       1050       1060       1070       1080 
AHLRASGAYR DAPEMAAQSP CSVPSQGSNP SFFSTPRECL PPFLGVPKQG LYSLGPPSFP 

      1090       1100       1110       1120       1130       1140 
PSSPAPVWRN SLGAPSALDR GENLYYEIGV GEGTSYSGPS RSWSPFRSMP PDRHNASYGM 

      1150       1160       1170       1180       1190       1200 
LGQSPPLHRS PDFLLSYPPP PSCFPPEHLT HSVSQRLARR PTRPEPLYVN LALGPRGPSP 

      1210       1220       1230       1240       1250       1260 
ASSSSSSPPA HPRSRSDPGP PVPRLPQKQR APWGPHTPHR VPGPWGSPEP FLLYRPAPPS 

      1270       1280       1290       1300 
YGRGGEVRGS LYRNGGHRGE GAGPPPPYPT PSWSLHSEGQ TRSYC 

« Hide

References

« Hide 'large scale' references
[1]"TCGAP, a multidomain Rho GTPase-activating protein involved in insulin-stimulated glucose transport."
Chiang S.-H., Hwang J., Legendre M., Zhang M., Kimura A., Saltiel A.R.
EMBO J. 22:2679-2691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH CDC42 AND RHOQ, POSSIBLE FUNCTION.
Tissue: Adipocyte.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 220-1305.
Strain: C57BL/6.
Tissue: Brain.
[3]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-594, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[4]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1188, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY217764 mRNA. Translation: AAO89073.1.
BC065086 mRNA. Translation: AAH65086.1.
BC065166 mRNA. Translation: AAH65166.1.
BC066047 mRNA. Translation: AAH66047.1.
BC061471 mRNA. Translation: AAH61471.1. Different initiation.
CCDSCCDS21095.1.
RefSeqNP_001276599.1. NM_001289670.1.
NP_001276611.1. NM_001289682.1.
NP_839983.1. NM_178252.3.
XP_006539951.1. XM_006539888.1.
UniGeneMm.190704.

3D structure databases

ProteinModelPortalQ80YF9.
SMRQ80YF9. Positions 221-272, 335-535.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid231366. 4 interactions.
IntActQ80YF9. 1 interaction.

PTM databases

PhosphoSiteQ80YF9.

Proteomic databases

PaxDbQ80YF9.
PRIDEQ80YF9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044338; ENSMUSP00000038412; ENSMUSG00000036882.
GeneID233071.
KEGGmmu:233071.
UCSCuc009geq.1. mouse.

Organism-specific databases

CTD115703.
MGIMGI:2673998. Arhgap33.

Phylogenomic databases

eggNOGNOG311367.
GeneTreeENSGT00720000108475.
HOVERGENHBG079688.
InParanoidQ80YF9.
KOK17933.
OMACFPPDHL.
OrthoDBEOG7WMCHV.
PhylomeDBQ80YF9.
TreeFamTF351451.

Gene expression databases

BgeeQ80YF9.
CleanExMM_SNX26.
GenevestigatorQ80YF9.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
InterProIPR001683. Phox.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTSM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEPS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio381531.
PROQ80YF9.
SOURCESearch...

Entry information

Entry nameRHG33_MOUSE
AccessionPrimary (citable) accession number: Q80YF9
Secondary accession number(s): Q6P7W6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot