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Q80YF9

- RHG33_MOUSE

UniProt

Q80YF9 - RHG33_MOUSE

Protein

Rho GTPase-activating protein 33

Gene

Arhgap33

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    May be involved in several stages of intracellular trafficking By similarity. Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.By similarity

    GO - Molecular functioni

    1. phosphatidylinositol binding Source: InterPro
    2. protein binding Source: MGI
    3. Rac GTPase activator activity Source: MGI
    4. Rho GTPase activator activity Source: MGI

    GO - Biological processi

    1. positive regulation of Rac GTPase activity Source: GOC
    2. positive regulation of Rho GTPase activity Source: GOC
    3. protein transport Source: UniProtKB-KW
    4. signal transduction Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_210090. Rho GTPase cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho GTPase-activating protein 33
    Alternative name(s):
    Rho-type GTPase-activating protein 33
    Sorting nexin-26
    Tc10/CDC42 GTPase-activating protein
    Gene namesi
    Name:Arhgap33
    Synonyms:Snx26, Tcgap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:2673998. Arhgap33.

    Subcellular locationi

    Cell membrane 1 Publication
    Note: Translocates to the plasma membrane in response to insulin in adipocytes.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13051305Rho GTPase-activating protein 33PRO_0000056722Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei594 – 5941Phosphoserine1 Publication
    Modified residuei660 – 6601PhosphoserineBy similarity
    Modified residuei1188 – 11881Phosphotyrosine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ80YF9.
    PRIDEiQ80YF9.

    PTM databases

    PhosphoSiteiQ80YF9.

    Expressioni

    Tissue specificityi

    Highly expressed in brain and testis. Also expressed in white adipose tissue (WAT) and muscle at a low level.1 Publication

    Inductioni

    Dramatically induced during adipocyte differentiation.1 Publication

    Gene expression databases

    BgeeiQ80YF9.
    CleanExiMM_SNX26.
    GenevestigatoriQ80YF9.

    Interactioni

    Subunit structurei

    Specifically interacts with CDC42 and RHOQ/TC10 through its Rho-GAP domain. Interacts with NEK6 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi231366. 4 interactions.
    IntActiQ80YF9. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80YF9.
    SMRiQ80YF9. Positions 221-272, 335-535.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini83 – 192110PX; atypicalAdd
    BLAST
    Domaini210 – 27263SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini339 – 534196Rho-GAPPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi150 – 1534Poly-Pro
    Compositional biasi701 – 72121Poly-SerAdd
    BLAST
    Compositional biasi909 – 9135Poly-Pro
    Compositional biasi1158 – 11614Poly-Pro
    Compositional biasi1202 – 12076Poly-Ser
    Compositional biasi1284 – 12918Poly-Pro

    Domaini

    The N-terminal PX domain interacts specifically with phosphatidylinositol 4,5-bisphosphate.

    Sequence similaritiesi

    Belongs to the PX domain-containing GAP family.Curated
    Contains 1 PX (phox homology) domain.Curated
    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiNOG311367.
    GeneTreeiENSGT00720000108475.
    HOVERGENiHBG079688.
    InParanoidiQ80YF9.
    KOiK17933.
    OMAiCFPPDHL.
    OrthoDBiEOG7WMCHV.
    PhylomeDBiQ80YF9.
    TreeFamiTF351451.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    InterProiIPR001683. Phox.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00620. RhoGAP. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    SMARTiSM00324. RhoGAP. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF64268. SSF64268. 1 hit.
    PROSITEiPS50238. RHOGAP. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80YF9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLQAQKQSDP ILPWGASWAG RGQTLRARST DSLDGPGEGS VQPVPTTGGP     50
    GTKGKPGKRL SAPRGPFPRL ADCAHFHYEN VDFGHIQLLL SPEREGPSLS 100
    GENELVFGVQ VTCQGRSWPV LRSYDDFRSL DAHLHRCIFD RRFSCLPELP 150
    PPPEGTRAAQ MLVPLLLQYL ETLSGLVDSN LNCGPVLTWM ELDNHGRRLL 200
    LSEEASLNIP AVAAAHVVKR YTAQAPDELS FEVGDIVSVI DMPPTEDRSW 250
    WRGKRGFQVG FFPSECVELF TERPGPGLKA DADSPLCGIP APQGISSLTS 300
    AVPRPRGKLA GLLRTFMRSR PSRQRLRQRG ILRQRVFGCD LGEHLSNSGQ 350
    DVPQVLRCCS EFIEAHGVVD GIYRLSGVSS NIQRLRHEFD SERIPELSGP 400
    AFLQDIHSVS SLCKLYFREL PNPLLTYQLY GKFSEAMSVP GEEERLVRVH 450
    DVIQQLPPPH YRTLEYLLRH LARMARHSAN TSMHARNLAI VWAPNLLRSM 500
    ELESVGLGGA AAFREVRVQS VVVEFLLTHV EVLFSDTFTS AGLDPAGRCL 550
    LPRPKSLAGS SPSTRLLTLE EAQARTQGRL GTPTEPTTPK TPASPVERRK 600
    RERAEKQRKP GGSSWKTFFA LGRGPSIPRK KPLPWLGGSR APPQPSGSRP 650
    DTVTLRSAKS EESLSSQASG AGLQRLHRLR RPHSSSDAFP VGPAPAGSCE 700
    SLSSSSSSSS SSSSSSSSES SAGGLGPLSG SPSHRTSAWL DDGDDLDFSP 750
    PRCLEGLRGL DFDPLTFRCS SPTPGDPAPP ASPAPPASAS AFPPRATPQA 800
    LSPHGPTKPA SPTALDISEP LAVSVPPAVL ELLGAGGTPA SATPTPALSP 850
    HLIPLLLRGA EAQLSDTCQQ EISSKLAPTR GAPGQQSPGG MDSPLLPPPL 900
    PLLRPGGAPP PPPKNPARLM ALALAERAQQ VAEQQSQQEQ GGTPPAPHSP 950
    FRRSLSLEVG GEPVGTSGSG IHPPSLAHPG AWAPGPPPYL PRQQSDGSLV 1000
    RSQRPLGTSR RSPRGPSQVS AHLRASGAYR DAPEMAAQSP CSVPSQGSNP 1050
    SFFSTPRECL PPFLGVPKQG LYSLGPPSFP PSSPAPVWRN SLGAPSALDR 1100
    GENLYYEIGV GEGTSYSGPS RSWSPFRSMP PDRHNASYGM LGQSPPLHRS 1150
    PDFLLSYPPP PSCFPPEHLT HSVSQRLARR PTRPEPLYVN LALGPRGPSP 1200
    ASSSSSSPPA HPRSRSDPGP PVPRLPQKQR APWGPHTPHR VPGPWGSPEP 1250
    FLLYRPAPPS YGRGGEVRGS LYRNGGHRGE GAGPPPPYPT PSWSLHSEGQ 1300
    TRSYC 1305
    Length:1,305
    Mass (Da):139,801
    Last modified:June 1, 2003 - v1
    Checksum:iAA8723BDF4A3CBB6
    GO

    Sequence cautioni

    The sequence AAH61471.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti357 – 3571R → C in AAH61471. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY217764 mRNA. Translation: AAO89073.1.
    BC065086 mRNA. Translation: AAH65086.1.
    BC065166 mRNA. Translation: AAH65166.1.
    BC066047 mRNA. Translation: AAH66047.1.
    BC061471 mRNA. Translation: AAH61471.1. Different initiation.
    CCDSiCCDS21095.1.
    RefSeqiNP_001276599.1. NM_001289670.1.
    NP_001276611.1. NM_001289682.1.
    NP_839983.1. NM_178252.3.
    XP_006539951.1. XM_006539888.1.
    UniGeneiMm.190704.

    Genome annotation databases

    EnsembliENSMUST00000044338; ENSMUSP00000038412; ENSMUSG00000036882.
    GeneIDi233071.
    KEGGimmu:233071.
    UCSCiuc009geq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY217764 mRNA. Translation: AAO89073.1 .
    BC065086 mRNA. Translation: AAH65086.1 .
    BC065166 mRNA. Translation: AAH65166.1 .
    BC066047 mRNA. Translation: AAH66047.1 .
    BC061471 mRNA. Translation: AAH61471.1 . Different initiation.
    CCDSi CCDS21095.1.
    RefSeqi NP_001276599.1. NM_001289670.1.
    NP_001276611.1. NM_001289682.1.
    NP_839983.1. NM_178252.3.
    XP_006539951.1. XM_006539888.1.
    UniGenei Mm.190704.

    3D structure databases

    ProteinModelPortali Q80YF9.
    SMRi Q80YF9. Positions 221-272, 335-535.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231366. 4 interactions.
    IntActi Q80YF9. 1 interaction.

    PTM databases

    PhosphoSitei Q80YF9.

    Proteomic databases

    PaxDbi Q80YF9.
    PRIDEi Q80YF9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000044338 ; ENSMUSP00000038412 ; ENSMUSG00000036882 .
    GeneIDi 233071.
    KEGGi mmu:233071.
    UCSCi uc009geq.1. mouse.

    Organism-specific databases

    CTDi 115703.
    MGIi MGI:2673998. Arhgap33.

    Phylogenomic databases

    eggNOGi NOG311367.
    GeneTreei ENSGT00720000108475.
    HOVERGENi HBG079688.
    InParanoidi Q80YF9.
    KOi K17933.
    OMAi CFPPDHL.
    OrthoDBi EOG7WMCHV.
    PhylomeDBi Q80YF9.
    TreeFami TF351451.

    Enzyme and pathway databases

    Reactomei REACT_210090. Rho GTPase cycle.

    Miscellaneous databases

    NextBioi 381531.
    PROi Q80YF9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q80YF9.
    CleanExi MM_SNX26.
    Genevestigatori Q80YF9.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    InterProi IPR001683. Phox.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00620. RhoGAP. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    SMARTi SM00324. RhoGAP. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF64268. SSF64268. 1 hit.
    PROSITEi PS50238. RHOGAP. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TCGAP, a multidomain Rho GTPase-activating protein involved in insulin-stimulated glucose transport."
      Chiang S.-H., Hwang J., Legendre M., Zhang M., Kimura A., Saltiel A.R.
      EMBO J. 22:2679-2691(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH CDC42 AND RHOQ, POSSIBLE FUNCTION.
      Tissue: Adipocyte.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 220-1305.
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-594, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    4. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1188, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiRHG33_MOUSE
    AccessioniPrimary (citable) accession number: Q80YF9
    Secondary accession number(s): Q6P7W6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3