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Protein

Connector enhancer of kinase suppressor of ras 2

Gene

Cnksr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as an adapter protein or regulator of Ras signaling pathways.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Connector enhancer of kinase suppressor of ras 2
Short name:
Connector enhancer of KSR 2
Alternative name(s):
CNK homolog protein 2
Short name:
CNK2
Gene namesi
Name:Cnksr2
Synonyms:Kiaa0902
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2661175. Cnksr2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10321032Connector enhancer of kinase suppressor of ras 2PRO_0000089971Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121PhosphoserineCombined sources
Modified residuei338 – 3381PhosphoserineCombined sources
Modified residuei390 – 3901PhosphoserineCombined sources
Modified residuei683 – 6831PhosphotyrosineCombined sources
Modified residuei685 – 6851PhosphoserineCombined sources
Modified residuei687 – 6871PhosphoserineCombined sources
Modified residuei756 – 7561PhosphoserineBy similarity
Modified residuei767 – 7671PhosphoserineBy similarity
Modified residuei906 – 9061PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated on tyrosine.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80YA9.
MaxQBiQ80YA9.
PaxDbiQ80YA9.
PeptideAtlasiQ80YA9.
PRIDEiQ80YA9.

PTM databases

iPTMnetiQ80YA9.
PhosphoSiteiQ80YA9.

Expressioni

Gene expression databases

BgeeiQ80YA9.
CleanExiMM_CNKSR2.
ExpressionAtlasiQ80YA9. baseline and differential.
GenevisibleiQ80YA9. MM.

Interactioni

Subunit structurei

Interacts with RAF1, RAB2L and RAL GTPase proteins.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ80YA9. 1 interaction.
STRINGi10090.ENSMUSP00000026750.

Structurei

3D structure databases

ProteinModelPortaliQ80YA9.
SMRiQ80YA9. Positions 6-80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 7666SAMPROSITE-ProRule annotationAdd
BLAST
Domaini84 – 17895CRICPROSITE-ProRule annotationAdd
BLAST
Domaini215 – 29783PDZPROSITE-ProRule annotationAdd
BLAST
Domaini302 – 515214DUF1170Add
BLAST
Domaini570 – 669100PHPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili874 – 91744Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi354 – 3574Poly-Pro
Compositional biasi703 – 7064Poly-Pro
Compositional biasi875 – 88612Poly-GluAdd
BLAST

Sequence similaritiesi

Belongs to the CNKSR family.Curated
Contains 1 CRIC domain.PROSITE-ProRule annotation
Contains 1 DUF1170 domain.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG4110T89. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000231501.
HOVERGENiHBG051040.
InParanoidiQ80YA9.
KOiK17536.
OMAiSTKLEYK.
OrthoDBiEOG71ZP0W.
PhylomeDBiQ80YA9.
TreeFamiTF326495.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR010599. CNKSR2.
IPR017874. CRIC_domain.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF10534. CRIC_ras_sig. 1 hit.
PF06663. DUF1170. 1 hit.
PF00595. PDZ. 1 hit.
PF00169. PH. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80YA9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALIMEPVSK WSPSQVVDWM KGLDDCLQQY IKNFEREKIS GDQLLRITHQ
60 70 80 90 100
ELEDLGVSRI GHQELILEAV DLLCALNYGL ETENLKTLSH KLNASAKNLQ
110 120 130 140 150
NFITGRRRSG HYDGRTSRKL PNDFLTSVVD LIGAAKSLLA WLDRSPFAAV
160 170 180 190 200
TDYSVTRNNV IQLCLELTTI VQQDCTVYET ENKILHVCKT LSGVCDHIIS
210 220 230 240 250
LSSDPLVSQS AHLEVIQLAN IKPSEGLGMY IKSTYDGLHV ITGTTENSPA
260 270 280 290 300
DRCKKIHAGD EVIQVNHQTV VGWQLKNLVN ALREDPSGVI LTLKKRPQSM
310 320 330 340 350
LTSAPALLKN MRWKPLALQP LIPRSPTSSV ATPSSTISTP TKRDSSALQD
360 370 380 390 400
LYIPPPPAEP YIPRDEKGNL PCEDLRGHMV GKPVHKGSES PNSFLDQEYR
410 420 430 440 450
KRFNIVEEDT VLYCYEYEKG RSSSQGRRES TPTYGKLRPI SMPVEYNWVG
460 470 480 490 500
DYEDPNKMKR DSRRENSLLR YMSNEKIAQE EYMFQRNSKK DTGKKSKKKG
510 520 530 540 550
DKSNSPAHYS LLPSLQMDAL RQDIMGTPVP ETTLYHTFQQ SSLQHKSKKK
560 570 580 590 600
NKGAISGKSK RRISCKDLGR GDCEGWLWKK KDAKSYFSQK WKKYWFVLKD
610 620 630 640 650
ASLYWYINEE DEKAEGFISL PEFKIDRASE CRKKYAFKAC HPKIKSFYFA
660 670 680 690 700
AEHLDDMNRW LNRINMLTAG YAERERIKQE QDYWSESDKE EADTPSTPKQ
710 720 730 740 750
DSPPPPYDTY PRPPSMSCAS PYVEAKHSRL SSTETSQSQS SHEEFRQEVT
760 770 780 790 800
GSSAVSPIRK TASQRRSWQD LIETPLTSSG LHYLQTLPLE DSVFSDSAAI
810 820 830 840 850
SPEHRRQSTL PTQKCHLQDH YGPYPLAESE RMQVLNGNGG KPRSFTLPRD
860 870 880 890 900
SGFNHCCLNT PVSACDPQDD IQPPEVEEEE EEEEEEAAGE NVGEKNENRE
910 920 930 940 950
EKLGDSLQDL YRALEEASLS PLGEHRISTK MEYKLSFIKR CNDPVMNEKL
960 970 980 990 1000
HRLRILKSTL KAREGEVAII DKVLDNPDLT SKEFQQWKQM YLDLFLDICQ
1010 1020 1030
STTSNDPLSI SSEVDVLTSS LTHTHSYIET HV
Length:1,032
Mass (Da):117,396
Last modified:June 1, 2003 - v1
Checksum:iFE97ED5E3CDB75BF
GO
Isoform 2 (identifier: Q80YA9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     271-319: Missing.

Show »
Length:983
Mass (Da):111,889
Checksum:i71BC4065E372BFF8
GO

Sequence cautioni

The sequence BAC65681.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei271 – 31949Missing in isoform 2. 1 PublicationVSP_010890Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122399 mRNA. Translation: BAC65681.1. Different initiation.
BC043093 mRNA. Translation: AAH43093.1.
BC060716 mRNA. Translation: AAH60716.1.
CCDSiCCDS30501.1. [Q80YA9-1]
RefSeqiNP_001297648.1. NM_001310719.1. [Q80YA9-2]
NP_808419.1. NM_177751.3. [Q80YA9-1]
UniGeneiMm.197074.

Genome annotation databases

EnsembliENSMUST00000026750; ENSMUSP00000026750; ENSMUSG00000025658. [Q80YA9-1]
GeneIDi245684.
KEGGimmu:245684.
UCSCiuc009ush.1. mouse. [Q80YA9-1]
uc009usi.1. mouse. [Q80YA9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122399 mRNA. Translation: BAC65681.1. Different initiation.
BC043093 mRNA. Translation: AAH43093.1.
BC060716 mRNA. Translation: AAH60716.1.
CCDSiCCDS30501.1. [Q80YA9-1]
RefSeqiNP_001297648.1. NM_001310719.1. [Q80YA9-2]
NP_808419.1. NM_177751.3. [Q80YA9-1]
UniGeneiMm.197074.

3D structure databases

ProteinModelPortaliQ80YA9.
SMRiQ80YA9. Positions 6-80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80YA9. 1 interaction.
STRINGi10090.ENSMUSP00000026750.

PTM databases

iPTMnetiQ80YA9.
PhosphoSiteiQ80YA9.

Proteomic databases

EPDiQ80YA9.
MaxQBiQ80YA9.
PaxDbiQ80YA9.
PeptideAtlasiQ80YA9.
PRIDEiQ80YA9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026750; ENSMUSP00000026750; ENSMUSG00000025658. [Q80YA9-1]
GeneIDi245684.
KEGGimmu:245684.
UCSCiuc009ush.1. mouse. [Q80YA9-1]
uc009usi.1. mouse. [Q80YA9-2]

Organism-specific databases

CTDi22866.
MGIiMGI:2661175. Cnksr2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1738. Eukaryota.
ENOG4110T89. LUCA.
GeneTreeiENSGT00390000017199.
HOGENOMiHOG000231501.
HOVERGENiHBG051040.
InParanoidiQ80YA9.
KOiK17536.
OMAiSTKLEYK.
OrthoDBiEOG71ZP0W.
PhylomeDBiQ80YA9.
TreeFamiTF326495.

Miscellaneous databases

PROiQ80YA9.
SOURCEiSearch...

Gene expression databases

BgeeiQ80YA9.
CleanExiMM_CNKSR2.
ExpressionAtlasiQ80YA9. baseline and differential.
GenevisibleiQ80YA9. MM.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR010599. CNKSR2.
IPR017874. CRIC_domain.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF10534. CRIC_ras_sig. 1 hit.
PF06663. DUF1170. 1 hit.
PF00595. PDZ. 1 hit.
PF00169. PH. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS51290. CRIC. 1 hit.
PS50106. PDZ. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-906, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-338; SER-390; TYR-683; SER-685; SER-687 AND SER-906, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Lung.

Entry informationi

Entry nameiCNKR2_MOUSE
AccessioniPrimary (citable) accession number: Q80YA9
Secondary accession number(s): Q80TP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2003
Last modified: July 6, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.