Reviewed, UniProtKB/Swiss-Prot Q80YA7 (DPP8_MOUSE)

Names and origin

Protein names

Recommended name:
    Dipeptidyl peptidase 8
    EC=3.4.14.5
Alternative name(s):
    Dipeptidyl peptidase VIII
    DP8

Gene names

Name:

Dpp8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	892 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function By similarity.
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Enzyme regulation	Inhibited by zinc. Inhibited by the serine proteinase inhibitor 4-(2-aminoethyl)benzenesulphonyl fluoride (AEBSF), and by di-isopropylfuorophosphate. Specifically inhibited by isoindoline derivatives By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase S9B family. DPPIV subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Aminopeptidase Hydrolase Protease Serine protease
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 892	892	Dipeptidyl peptidase 8		PRO_0000122414
Sites
	Active site	749	1	Charge relay system By similarity
	Active site	827	1	Charge relay system By similarity
	Active site	859	1	Charge relay system By similarity
Amino acid modifications
	Modified residue	325	1	Phosphotyrosine Ref.3
	Modified residue	328	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	87	1	G → R in BAB30295. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q80YA7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 59081CD9792E03ED	FASTA	892	102,186
	10 20 30 40 50 60 MKIPSGRCNM AAAMETEQLG VEIFETAECE EGNGESQDRP KLEPFYVERY SWSQLKKLLA 70 80 90 100 110 120 DTRKYHGYMM AKAPHDFMFV KRTDPDGPHS DRVYYLAMSG ENRENTLFYS EIPKTINRAA 130 140 150 160 170 180 VLMLSWKPLL DLFQATLDYG MYSREEELLR ERKRIGTVGI AAYDYHPGSG TFLFQAGSGI 190 200 210 220 230 240 YHIKDGGPHG FTQQPLRPNL VETSCPNIRM DPKLCPADPD WIAFIHSNDI WISNLVTREE 250 260 270 280 290 300 RRITYVHNEL ANMEEDPRSA GVATFVLQEE FDRYSGYWWC PQAERTPSGG KILRILYEEN 310 320 330 340 350 360 DESEVEIIHV TSPMLETRRA DSFRYPKTGT ANPKVTFKMS EIVVDAAGGI IDVIDKELVQ 370 380 390 400 410 420 PFEILFEGVE YIARAGWTPE GKHAWSILLD RSQTHLQIVL ISPELFIPVE DDAMDRQRLI 430 440 450 460 470 480 ESVPDSVTPL IIYEETTDIW INIHDIFHVF PQTHEDEIEF IFASECKTGF RHLYKITSIL 490 500 510 520 530 540 KESKYKRSSG GLPAPSDFKC PIKEEITITS GEWEVLGRHG SNIWVDEARK LVYFEGTKDS 550 560 570 580 590 600 PLEHHLYVTS YANPGEVVRL TDRGYSHSCC LSRHCDFFIS KYSNQKNPHC VSLYKLSSPE 610 620 630 640 650 660 DDPVHKTKEF WATILDSAGP LPDYTPPEIF SFESTTGFTL YGMLYKPHDL QPGKKYPTVL 670 680 690 700 710 720 FIYGGPQVQL VNNRFKGVKY FRLNTLASLG YVVVVIDNRG SCHRGLKFEG AFKYKMGQIE 730 740 750 760 770 780 IDDQVEGLQY LASQYDFIDL DRVGIHGWSY GGYLSLMALM QRSDIFRVAI AGAPVTLWIF 790 800 810 820 830 840 YDTGYTERYM GHPDQNEQGY YLGSVAMQAE KFPSEPNRLL LLHGFLDENV HFAHTSILLS 850 860 870 880 890 FLVRAGKPYD LQIYPQERHS IRVPESGEHY ELHLLHYLQE NLGSRIAALK VI

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line." Shu H., Chen S., Bi Q., Mumby M., Brekken D.L. Mol. Cell. Proteomics 3:279-286(2004) [PubMed: 14729942] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-325, MASS SPECTROMETRY. Tissue: B-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK016546 mRNA. Translation: BAB30295.2. BC043124 mRNA. Translation: AAH43124.1. BC059222 mRNA. Translation: AAH59222.1.
IPI	IPI00330837.
RefSeq	NP_083182.2.
UniGene	Mm.202749 Mm.391842
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q80YA7.
PTM databases
PhosphoSite	Q80YA7.
Proteomic databases
PRIDE	Q80YA7.
Genome annotation databases
Ensembl	ENSMUST00000034960; ENSMUSP00000034960; ENSMUSG00000032393; Mus musculus. [Genome view]
GeneID	74388.
KEGG	mmu:74388.
UCSC	uc009qcq.1. mouse.
Organism-specific databases
CTD	74388.
MGI	MGI:1921638. Dpp8.
Phylogenomic databases
HOGENOM	Q80YA7.
HOVERGEN	Q80YA7.
OMA	YPGSKHS.
Enzyme and pathway databases
BRENDA	3.4.14.5. 244.
Gene expression databases
ArrayExpress	Q80YA7.
Bgee	Q80YA7.
CleanEx	MM_DPP8.
Genevestigator	Q80YA7.
GermOnline	ENSMUSG00000032393. Mus musculus.
Family and domain databases
InterPro	IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	340625.
SOURCE	Search...

Entry information

Entry name

DPP8_MOUSE

Accession

Primary (citable) accession number: Q80YA7
Secondary accession number(s): Q9D4G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)