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Protein

Phospholipase DDHD2

Gene

Ddhd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phospholipase that hydrolyzes preferentially phosphatidic acid, including 1,2-dioleoyl-sn-phosphatidic acid, and phosphatidylethanolamine. Specifically binds to phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P), phosphatidylinositol 5-phosphate (PI5P) and possibly phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). May be involved in the maintenance of the endoplasmic reticulum and/or Golgi structures. May regulate the transport between Golgi apparatus and plasma membrane (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei351Curated1

GO - Molecular functioni

  • metal ion binding Source: InterPro
  • phospholipase activity Source: GO_Central
  • triglyceride lipase activity Source: MGI

GO - Biological processi

  • ER to Golgi vesicle-mediated transport Source: GO_Central
  • lipid particle organization Source: MGI
  • locomotory behavior Source: MGI
  • positive regulation of mitochondrial fission Source: MGI
  • triglyceride catabolic process Source: MGI
  • visual learning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1483166. Synthesis of PA.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase DDHD2 (EC:3.1.1.-)
Alternative name(s):
DDHD domain-containing protein 2
SAM, WWE and DDHD domain-containing protein 1
Gene namesi
Name:Ddhd2
Synonyms:Kiaa0725, Samwd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1919358. Ddhd2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3259495.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003093311 – 699Phospholipase DDHD2Add BLAST699

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei447PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80Y98.
PaxDbiQ80Y98.
PeptideAtlasiQ80Y98.
PRIDEiQ80Y98.

PTM databases

iPTMnetiQ80Y98.
PhosphoSitePlusiQ80Y98.

Expressioni

Gene expression databases

BgeeiENSMUSG00000061313.
CleanExiMM_DDHD2.
GenevisibleiQ80Y98. MM.

Interactioni

Subunit structurei

Forms homooligomers and, to a much smaller extent, heterooligomers with DDHD1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033975.

Structurei

3D structure databases

ProteinModelPortaliQ80Y98.
SMRiQ80Y98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 112WWEPROSITE-ProRule annotationAdd BLAST83
Domaini383 – 445SAMAdd BLAST63
Domaini484 – 688DDHDPROSITE-ProRule annotationAdd BLAST205

Domaini

SAM and DDHD domains together are required for phospholipid binding.By similarity

Sequence similaritiesi

Belongs to the PA-PLA1 family.Curated
Contains 1 DDHD domain.PROSITE-ProRule annotation
Contains 1 WWE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2308. Eukaryota.
ENOG410YDSX. LUCA.
GeneTreeiENSGT00530000063155.
HOGENOMiHOG000007725.
HOVERGENiHBG057256.
InParanoidiQ80Y98.
KOiK16545.
OMAiMSASEVN.
OrthoDBiEOG091G02T5.
TreeFamiTF314133.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR004177. DDHD_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR004170. WWE-dom.
[Graphical view]
PfamiPF02862. DDHD. 1 hit.
PF00536. SAM_1. 1 hit.
PF02825. WWE. 1 hit.
[Graphical view]
SMARTiSM01127. DDHD. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS51043. DDHD. 1 hit.
PS50918. WWE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80Y98-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSGESHQEQ LSQSDPSPSP NSCSSFELID MDASSSYEPV SPHWFYCKVL
60 70 80 90 100
DSKELWIPFN SEDSQQLEDA YGSGKDCNER IVPTDGGRYD VHLGERMRYA
110 120 130 140 150
VYWDELPSEV RRCTWFYKGD KDNKYVPYSE SFSQVLEDTY MLAVTLDEWK
160 170 180 190 200
KKIESPNREI IVLHNPKLMV HYQPIAGSDE WGSTSTEQGR PRSVKRGVEN
210 220 230 240 250
IPVDIHCGEP LQIDHLVFVV HGIGPACDLR FRSIVQCVND FRSVSLNLLQ
260 270 280 290 300
THFKKAQENE QIGRVEFLPV NWHSPLHSTG VDIDLQRITL PSINRLRHFT
310 320 330 340 350
NDTILDVFFY NSPTYCQTIV DTVASEMNRI YTLFLQRNPD FKGGVSIAGH
360 370 380 390 400
SLGSLILFDI LTNQKNSIGD IDSEKGSLSS AEDRGDASTL EEDLKKLQLS
410 420 430 440 450
EFVTVFEKEK VDREALALCT DRDLQEMGIP LGPRKKILNH FSARKNSVSI
460 470 480 490 500
NRPAMSASEV NISKENGDYL DVGIGQVSVK YPRLNYKPEI FFAFGSPIGM
510 520 530 540 550
FLTVRGLRRI DPNYKFPTCK GFFNIYHPFD PVAYRIEPMV APGIEFEPML
560 570 580 590 600
IPHHKGRKRM HLELREGLTR MSMDLKNNLL GSLRMAWKSF TRGPYPALQA
610 620 630 640 650
SETAEETEAE PESSSEKSNE ANTEEPPVEV KEEAPISVGM LNGGQRIDYV
660 670 680 690
LQEKPIESFN EYLFALQSHL CYWESEDTVL LVLKEIYQTQ GVFLDQPLQ
Length:699
Mass (Da):79,577
Last modified:July 27, 2011 - v3
Checksum:iBB12F07F9200ACC7
GO
Isoform 2 (identifier: Q80Y98-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRGQKVRFSS...PPRPAWESEM
     448-528: Missing.

Note: No experimental confirmation available.
Show »
Length:730
Mass (Da):82,127
Checksum:i73AB101005C906DB
GO
Isoform 3 (identifier: Q80Y98-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     283-382: IDLQRITLPS...SEKGSLSSAE → M
     529-562: Missing.

Note: No experimental confirmation available.
Show »
Length:566
Mass (Da):64,547
Checksum:iF7D7A60CE18B8536
GO

Sequence cautioni

The sequence AAH46229 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAI18963 differs from that shown. Reason: Frameshift at position 149.Curated
The sequence BAD32288 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti637S → N in BAD32288 (PubMed:15368895).Curated1
Sequence conflicti637S → N in AAI18963 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0291411M → MRGQKVRFSSFKASAQARPP VRVPHAPSACPARARRPTSA RRRSQVSRESPSPHRTSRDT SEDLSAPPALTGSAASAGAL LSTAGALRSPRCGDWGAAAG SARPPRPAWESEM in isoform 2. 1 Publication1
Alternative sequenceiVSP_029142283 – 382IDLQR…LSSAE → M in isoform 3. 1 PublicationAdd BLAST100
Alternative sequenceiVSP_029143448 – 528Missing in isoform 2. 1 PublicationAdd BLAST81
Alternative sequenceiVSP_029144529 – 562Missing in isoform 3. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173010 Transcribed RNA. Translation: BAD32288.1. Different initiation.
AC156990 Genomic DNA. No translation available.
BC046229 mRNA. Translation: AAH46229.1. Different initiation.
BC118962 mRNA. Translation: AAI18963.1. Frameshift.
AK008529 mRNA. Translation: BAB25722.1.
CCDSiCCDS52529.1. [Q80Y98-1]
RefSeqiNP_082378.1. NM_028102.1. [Q80Y98-1]
XP_006509260.2. XM_006509197.3. [Q80Y98-1]
XP_006509261.1. XM_006509198.3. [Q80Y98-1]
UniGeneiMm.246875.

Genome annotation databases

EnsembliENSMUST00000033975; ENSMUSP00000033975; ENSMUSG00000061313. [Q80Y98-1]
GeneIDi72108.
KEGGimmu:72108.
UCSCiuc009lgv.2. mouse. [Q80Y98-1]
uc009lgw.2. mouse. [Q80Y98-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173010 Transcribed RNA. Translation: BAD32288.1. Different initiation.
AC156990 Genomic DNA. No translation available.
BC046229 mRNA. Translation: AAH46229.1. Different initiation.
BC118962 mRNA. Translation: AAI18963.1. Frameshift.
AK008529 mRNA. Translation: BAB25722.1.
CCDSiCCDS52529.1. [Q80Y98-1]
RefSeqiNP_082378.1. NM_028102.1. [Q80Y98-1]
XP_006509260.2. XM_006509197.3. [Q80Y98-1]
XP_006509261.1. XM_006509198.3. [Q80Y98-1]
UniGeneiMm.246875.

3D structure databases

ProteinModelPortaliQ80Y98.
SMRiQ80Y98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033975.

Chemistry databases

ChEMBLiCHEMBL3259495.

PTM databases

iPTMnetiQ80Y98.
PhosphoSitePlusiQ80Y98.

Proteomic databases

EPDiQ80Y98.
PaxDbiQ80Y98.
PeptideAtlasiQ80Y98.
PRIDEiQ80Y98.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033975; ENSMUSP00000033975; ENSMUSG00000061313. [Q80Y98-1]
GeneIDi72108.
KEGGimmu:72108.
UCSCiuc009lgv.2. mouse. [Q80Y98-1]
uc009lgw.2. mouse. [Q80Y98-2]

Organism-specific databases

CTDi23259.
MGIiMGI:1919358. Ddhd2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2308. Eukaryota.
ENOG410YDSX. LUCA.
GeneTreeiENSGT00530000063155.
HOGENOMiHOG000007725.
HOVERGENiHBG057256.
InParanoidiQ80Y98.
KOiK16545.
OMAiMSASEVN.
OrthoDBiEOG091G02T5.
TreeFamiTF314133.

Enzyme and pathway databases

ReactomeiR-MMU-1483166. Synthesis of PA.

Miscellaneous databases

ChiTaRSiDdhd2. mouse.
PROiQ80Y98.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000061313.
CleanExiMM_DDHD2.
GenevisibleiQ80Y98. MM.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR004177. DDHD_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR004170. WWE-dom.
[Graphical view]
PfamiPF02862. DDHD. 1 hit.
PF00536. SAM_1. 1 hit.
PF02825. WWE. 1 hit.
[Graphical view]
SMARTiSM01127. DDHD. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS51043. DDHD. 1 hit.
PS50918. WWE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDHD2_MOUSE
AccessioniPrimary (citable) accession number: Q80Y98
Secondary accession number(s): E9QKK2
, Q0VF66, Q6A008, Q9CVE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-31 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.