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Protein

Rabenosyn-5

Gene

Rbsn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3). Plays a role in the recycling of transferrin receptor to the plasma membrane (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 3724C2H2-typeAdd
BLAST
Zinc fingeri156 – 259104FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • Rab GTPase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Rabenosyn-5By similarity
Alternative name(s):
FYVE finger-containing Rab5 effector protein rabenosyn-5
RAB effector RBSNBy similarity
Zinc finger FYVE domain-containing protein 20
Gene namesi
Name:RbsnBy similarity
Synonyms:Zfyve20Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1925537. Rbsn.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
  • Early endosome membrane By similarity; Lipid-anchor By similarity

  • Note: Enriched in endosomes that are in close proximity to clathrin-enriched regions at the cell surface (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 783782Rabenosyn-5PRO_0000098712Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei214 – 2141PhosphoserineCombined sources
Modified residuei229 – 2291PhosphoserineCombined sources
Modified residuei686 – 6861PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein

Proteomic databases

EPDiQ80Y56.
MaxQBiQ80Y56.
PaxDbiQ80Y56.
PRIDEiQ80Y56.

PTM databases

iPTMnetiQ80Y56.
PhosphoSiteiQ80Y56.

Expressioni

Gene expression databases

BgeeiQ80Y56.
CleanExiMM_ZFYVE20.
ExpressionAtlasiQ80Y56. baseline and differential.
GenevisibleiQ80Y56. MM.

Interactioni

Subunit structurei

Interacts with EHD1, RAB4A, RAB5A, RAB22A, RAB24 and VPS45. Binds simultaneously to RAB4A and RAB5A in vitro. Interacts with RAB4A and RAB5A that has been activated by GTP binding (By similarity).By similarity

GO - Molecular functioni

  • Rab GTPase binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000014694.

Structurei

3D structure databases

ProteinModelPortaliQ80Y56.
SMRiQ80Y56. Positions 153-189, 457-500, 733-783.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini495 – 51420UIMCuratedAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 262164Necessary for the correct targeting to endosomesBy similarityAdd
BLAST
Regioni263 – 783521Necessary for the interaction with EHD1By similarityAdd
BLAST
Regioni263 – 499237Necessary for the interaction with RAB4ABy similarityAdd
BLAST
Regioni627 – 783157Necessary for the interaction with RAB5ABy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili377 – 41236Sequence analysisAdd
BLAST
Coiled coili471 – 53161Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi203 – 23836Ser-richAdd
BLAST
Compositional biasi668 – 6714Poly-Glu
Compositional biasi680 – 71839Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 3724C2H2-typeAdd
BLAST
Zinc fingeri156 – 259104FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1842. Eukaryota.
ENOG410XP0U. LUCA.
GeneTreeiENSGT00390000007159.
HOGENOMiHOG000154082.
HOVERGENiHBG067237.
InParanoidiQ80Y56.
KOiK12481.
OMAiKLRLCME.
OrthoDBiEOG76MK7V.
PhylomeDBiQ80Y56.
TreeFamiTF106125.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR021565. Rbsn_Rab_binding_dom.
IPR007087. Znf_C2H2.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF11464. Rbsn. 2 hits.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 2 hits.
PROSITEiPS50178. ZF_FYVE. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80Y56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLDDPGEV REGFLCPLCL KDLQSFYQLQ SHYEEEHLED RDVKGQIKNL
60 70 80 90 100
VQKARKAKNK LLKREGDDRV EPGTQGYESF SYGGVDPYMW EPQELGAMRS
110 120 130 140 150
HLSDFKKHRA ARIDHYVVEV NKLIIRLEKL TAFDRTNTET SKIRAIEKSV
160 170 180 190 200
VPWVNDQDVP FCPDCGNKFS IRNRRHHCRL CGSIMCKKCM ELIGLPLAHK
210 220 230 240 250
LTSASKDSLS THTSPSQSPN SVHGSRRGSI SSMSSVSSVL DEKDDDRIRC
260 270 280 290 300
CTHCKDKLLK REQQMDEKEH TPDIVKLYEK LRLCMEKVDQ KAPEYIRMAA
310 320 330 340 350
SLNAGETTYN LEHANDLRVE VQKVYELIDA LSKKILTLGL NQDPSPHPNT
360 370 380 390 400
LRLQRMIRYS ATLFVQEKLL GLMSLPTKEQ FEELKKKRKQ DLEQKRTVER
410 420 430 440 450
QAALESRRKL EERQSGLASH TANGDVRSLR GIPPPLRKAE GWLPLSEGQG
460 470 480 490 500
QSEDPDPLLQ QIYNITSFIR QAKAAGRTDE VRTLQENLRQ LQDEYDQQQT
510 520 530 540 550
EKAIELSRKQ AEEEELQREQ LQMLRKRELE REQEQFLAAS LQTRTRVLEL
560 570 580 590 600
REVIPFQLEA SRGPHIDLSY SLDQDSSPVQ SSTAPDILTP GSALAPMHLW
610 620 630 640 650
SGPPALGQET LPQSTMSQQS DKASLNPFDE DDLSSPTEGA ISPAAVEAFL
660 670 680 690 700
GPPAAVTKEY NPFEEDAEEE EVAELGAGNP FTDPDSPAPN PFDEDDGPRP
710 720 730 740 750
ASPAAPGNPF EECPSTNPFE VDSDSGMEAE EHIEEELLLQ QIDNIKAYIF
760 770 780
DAKQCGRMDE VEVLTENLRE LKCTLAKQKG APN
Length:783
Mass (Da):88,491
Last modified:June 1, 2003 - v1
Checksum:i80103992F296CCD6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti227 – 2271R → Q in AAH31135 (PubMed:15489334).Curated
Sequence conflicti698 – 6981P → S in AAH31135 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031135 mRNA. Translation: AAH31135.1.
BC049106 mRNA. Translation: AAH49106.1.
AK019909 mRNA. Translation: BAB31910.1.
CCDSiCCDS20374.1.
RefSeqiNP_084357.2. NM_030081.2.
XP_006506831.1. XM_006506768.2.
UniGeneiMm.290734.

Genome annotation databases

EnsembliENSMUST00000014694; ENSMUSP00000014694; ENSMUSG00000014550.
GeneIDi78287.
KEGGimmu:78287.
UCSCiuc009cyv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031135 mRNA. Translation: AAH31135.1.
BC049106 mRNA. Translation: AAH49106.1.
AK019909 mRNA. Translation: BAB31910.1.
CCDSiCCDS20374.1.
RefSeqiNP_084357.2. NM_030081.2.
XP_006506831.1. XM_006506768.2.
UniGeneiMm.290734.

3D structure databases

ProteinModelPortaliQ80Y56.
SMRiQ80Y56. Positions 153-189, 457-500, 733-783.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000014694.

PTM databases

iPTMnetiQ80Y56.
PhosphoSiteiQ80Y56.

Proteomic databases

EPDiQ80Y56.
MaxQBiQ80Y56.
PaxDbiQ80Y56.
PRIDEiQ80Y56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014694; ENSMUSP00000014694; ENSMUSG00000014550.
GeneIDi78287.
KEGGimmu:78287.
UCSCiuc009cyv.1. mouse.

Organism-specific databases

CTDi64145.
MGIiMGI:1925537. Rbsn.

Phylogenomic databases

eggNOGiKOG1842. Eukaryota.
ENOG410XP0U. LUCA.
GeneTreeiENSGT00390000007159.
HOGENOMiHOG000154082.
HOVERGENiHBG067237.
InParanoidiQ80Y56.
KOiK12481.
OMAiKLRLCME.
OrthoDBiEOG76MK7V.
PhylomeDBiQ80Y56.
TreeFamiTF106125.

Enzyme and pathway databases

ReactomeiR-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

NextBioi348629.
PROiQ80Y56.
SOURCEiSearch...

Gene expression databases

BgeeiQ80Y56.
CleanExiMM_ZFYVE20.
ExpressionAtlasiQ80Y56. baseline and differential.
GenevisibleiQ80Y56. MM.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR021565. Rbsn_Rab_binding_dom.
IPR007087. Znf_C2H2.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF11464. Rbsn. 2 hits.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 2 hits.
PROSITEiPS50178. ZF_FYVE. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and FVB/N.
    Tissue: Brain and Kidney.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-384.
    Strain: C57BL/6J.
    Tissue: Pituitary.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214 AND SER-229, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Liver, Lung and Testis.

Entry informationi

Entry nameiRBNS5_MOUSE
AccessioniPrimary (citable) accession number: Q80Y56
Secondary accession number(s): Q8K0L6, Q9CTW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.