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Protein

Probable ATP-dependent RNA helicase DDX10

Gene

Ddx10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Putative ATP-dependent RNA helicase.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi89 – 913ATPPROSITE-ProRule annotation
Nucleotide bindingi113 – 1208ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. poly(A) RNA binding Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX10 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 10
Gene namesi
Name:Ddx10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1924841. Ddx10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 875875Probable ATP-dependent RNA helicase DDX10PRO_0000055084Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41PhosphothreonineBy similarity
Modified residuei540 – 5401PhosphoserineBy similarity
Modified residuei556 – 5561N6-acetyllysine1 Publication
Modified residuei587 – 5871Phosphothreonine2 Publications
Modified residuei783 – 7831PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ80Y44.
PaxDbiQ80Y44.
PRIDEiQ80Y44.

PTM databases

PhosphoSiteiQ80Y44.

Expressioni

Gene expression databases

BgeeiQ80Y44.
CleanExiMM_DDX10.
ExpressionAtlasiQ80Y44. baseline and differential.
GenevestigatoriQ80Y44.

Interactioni

Protein-protein interaction databases

BioGridi218780. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ80Y44.
SMRiQ80Y44. Positions 47-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 274175Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini300 – 449150Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 9729Q motifAdd
BLAST
Motifi222 – 2254DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00550000074980.
HOGENOMiHOG000268801.
HOVERGENiHBG102756.
InParanoidiQ80Y44.
KOiK14776.
OMAiTSHNRKK.
OrthoDBiEOG7GFB4K.
PhylomeDBiQ80Y44.
TreeFamiTF315215.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80Y44-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKTVASLGQ GTRPDPVRSF NRWKKKHSHR QHQKKERRKQ LKKPEWQVER
60 70 80 90 100
EGISRLMQNY EKINVNEITR FSDFPLSKKT LKGLQEAQYR LVTEIQKQTI
110 120 130 140 150
GLALQGKDVL GAAKTGSGKT LAFLVPVLEA LYRLQWTSTD GLGVLIISPT
160 170 180 190 200
RELAYQTFEV LRKVGKNHDF SAGLIIGGKD LKHEAERINN INILVCTPGR
210 220 230 240 250
LLQHMDETIC FHATNLQMLV LDEADRILDM GFADTMNAII ENLPKKRQTL
260 270 280 290 300
LFSATQTKSV KDLARLSLKD PEYVWVHEKA KYSTPATLEQ NYIICELHQK
310 320 330 340 350
ISVLFSFLRS HLKKKSIVFF SSCKEVQYLY RVFCRLRPGI SILALHGRQQ
360 370 380 390 400
QMRRMEVYNE FVRKRAAVLF ATDIAARGLD FPAVNWVLQF DCPEDANTYI
410 420 430 440 450
HRAGRTARYK EDGEALLILL PSEEQGMVQQ LLQKKVPVKE IKINPEKLID
460 470 480 490 500
VQKKLESFLA QDQDLKERAQ RCFVSYIRSV YLMKDKEVFN VSKLPITEYA
510 520 530 540 550
LSLGLAVAPR IRFLQKLEKQ PSTELVKNPV TEAVPPRAPS LPNDEADESP
560 570 580 590 600
AYVSEKMSVL HKSGERLEET EHRLASGDGD EEQDEETEDE ETEDHLGKAR
610 620 630 640 650
EPHTESVVSI EEAQKVKEVS VQFLNRDDDD EDGPDADFLT VKRRDVFGLD
660 670 680 690 700
LKENEALSKK EPSKSSVKKK LTKVAEAKKV MKRSFKVNKK ITFTDEGELV
710 720 730 740 750
QQWPQIQKCA IKDVEEEDDT GGINLDKAKE RLQEEDKFDK EEYRKKIKAK
760 770 780 790 800
HRERRLKERE ARREANKRQA KARDEEEAFL DWSDEDDGGF DPSTLPDPDK
810 820 830 840 850
HRSSEESESE DTNHKMSDTK KKQETRKRNN TEDDDVRPRS RHGKKAKWET
860 870
VEPLDTGLSL AEDEELVLHL LKSQN
Length:875
Mass (Da):100,739
Last modified:September 27, 2005 - v2
Checksum:i07D6574185AC3646
GO

Sequence cautioni

The sequence AAH49261.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC049261 mRNA. Translation: AAH49261.1. Different initiation.
CCDSiCCDS52796.1.
RefSeqiNP_084212.2. NM_029936.2.
UniGeneiMm.133560.

Genome annotation databases

EnsembliENSMUST00000065630; ENSMUSP00000065198; ENSMUSG00000053289.
GeneIDi77591.
KEGGimmu:77591.
UCSCiuc009plz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC049261 mRNA. Translation: AAH49261.1. Different initiation.
CCDSiCCDS52796.1.
RefSeqiNP_084212.2. NM_029936.2.
UniGeneiMm.133560.

3D structure databases

ProteinModelPortaliQ80Y44.
SMRiQ80Y44. Positions 47-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218780. 1 interaction.

PTM databases

PhosphoSiteiQ80Y44.

Proteomic databases

MaxQBiQ80Y44.
PaxDbiQ80Y44.
PRIDEiQ80Y44.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065630; ENSMUSP00000065198; ENSMUSG00000053289.
GeneIDi77591.
KEGGimmu:77591.
UCSCiuc009plz.2. mouse.

Organism-specific databases

CTDi1662.
MGIiMGI:1924841. Ddx10.

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00550000074980.
HOGENOMiHOG000268801.
HOVERGENiHBG102756.
InParanoidiQ80Y44.
KOiK14776.
OMAiTSHNRKK.
OrthoDBiEOG7GFB4K.
PhylomeDBiQ80Y44.
TreeFamiTF315215.

Miscellaneous databases

ChiTaRSiDdx10. mouse.
NextBioi347162.
PROiQ80Y44.
SOURCEiSearch...

Gene expression databases

BgeeiQ80Y44.
CleanExiMM_DDX10.
ExpressionAtlasiQ80Y44. baseline and differential.
GenevestigatoriQ80Y44.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-587, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  3. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-587, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-556, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiDDX10_MOUSE
AccessioniPrimary (citable) accession number: Q80Y44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 27, 2005
Last modified: February 4, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.