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Protein

Prickle-like protein 2

Gene

Prickle2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

  • establishment or maintenance of epithelial cell apical/basal polarity Source: MGI
  • neuron projection development Source: MGI
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Prickle-like protein 2
Gene namesi
Name:Prickle2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1925144. Prickle2.

Subcellular locationi

GO - Cellular componenti

  • apicolateral plasma membrane Source: MGI
  • cytoplasm Source: MGI
  • lateral plasma membrane Source: MGI
  • nuclear membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Null mice are viable, but show an increased seizure rate compared to heterozygous mice, indicating a dosage effect. Heterozygous Prickle2 +/- mice have a decreased seizure threshold compared to wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 842842Prickle-like protein 2PRO_0000075892Add
BLAST
Propeptidei843 – 8453Removed in mature formBy similarityPRO_0000396719

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei92 – 921PhosphoserineCombined sources
Modified residuei319 – 3191PhosphoserineCombined sources
Modified residuei321 – 3211PhosphoserineCombined sources
Modified residuei322 – 3221PhosphoserineCombined sources
Modified residuei535 – 5351PhosphothreonineCombined sources
Modified residuei537 – 5371PhosphothreonineCombined sources
Modified residuei540 – 5401PhosphothreonineCombined sources
Modified residuei544 – 5441PhosphoserineCombined sources
Modified residuei547 – 5471PhosphoserineCombined sources
Modified residuei608 – 6081PhosphoserineCombined sources
Modified residuei643 – 6431PhosphoserineCombined sources
Modified residuei732 – 7321PhosphoserineCombined sources
Modified residuei842 – 8421Cysteine methyl esterBy similarity
Lipidationi842 – 8421S-farnesyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ80Y24.
PaxDbiQ80Y24.
PRIDEiQ80Y24.

PTM databases

iPTMnetiQ80Y24.
PhosphoSiteiQ80Y24.

Expressioni

Tissue specificityi

Expressed in the hippocampus and cerebral cortex.1 Publication

Gene expression databases

BgeeiQ80Y24.
CleanExiMM_PRICKLE2.
ExpressionAtlasiQ80Y24. baseline and differential.
GenevisibleiQ80Y24. MM.

Interactioni

Protein-protein interaction databases

BioGridi232537. 1 interaction.
STRINGi10090.ENSMUSP00000032093.

Structurei

3D structure databases

ProteinModelPortaliQ80Y24.
SMRiQ80Y24. Positions 128-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 126109PETPROSITE-ProRule annotationAdd
BLAST
Domaini128 – 19366LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini193 – 25361LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini253 – 31765LIM zinc-binding 3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi504 – 5118Poly-Glu
Compositional biasi669 – 73163Arg-richAdd
BLAST
Compositional biasi780 – 7856Poly-Ser

Sequence similaritiesi

Belongs to the prickle / espinas / testin family.Curated
Contains 3 LIM zinc-binding domains.PROSITE-ProRule annotation
Contains 1 PET domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1704. Eukaryota.
ENOG410XP0W. LUCA.
GeneTreeiENSGT00550000074438.
HOGENOMiHOG000290649.
HOVERGENiHBG053679.
InParanoidiQ80Y24.
KOiK04511.
OrthoDBiEOG7P8P7M.
TreeFamiTF313265.

Family and domain databases

Gene3Di2.10.110.10. 3 hits.
InterProiIPR010442. PET_domain.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 3 hits.
PF06297. PET. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS51303. PET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80Y24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTVMPLEME KTISKLMFDF QRSSTSDDDS GCALEEYAWV PPGLKPEQVH
60 70 80 90 100
QYYSCLPEEK VPYVNSAGEK LRIKQLLHQL PPHDNEVRYC NSLDEEEKRE
110 120 130 140 150
LKLFSNQRKR ENLGRGNVRP FPVTMTGAIC EQCGGQIKGG DIAVFASRAG
160 170 180 190 200
HGICWHPPCF VCTVCNELLV DLIYFYQDGK IYCGRHHAEC LKPRCAACDE
210 220 230 240 250
IIFADECTEA EGRHWHMRHF CCFECETVLG GQRYIMKEGR PYCCHCFESL
260 270 280 290 300
YAEYCDTCAQ HIGIDQGQMT YDGQHWHATE TCFCCAHCKK SLLGRPFLPK
310 320 330 340 350
QGQIFCSRAC SAGEDPNGSD SSDSAFQNAR AKESRRSAKI GKNKGKTEEA
360 370 380 390 400
MLNQHSQLQV SSNRLSADVD PLSVQMDLLS LSSQTPSLNR DPIWRSREEP
410 420 430 440 450
FHYGNKMEQN QSQSPLQLLS QCNIRTSYSP GGQGAGAQPD MWAKHFSNPK
460 470 480 490 500
RSSSMALKGH GGSFIQECRE DYYPGRLMSQ ESYSDMSSQS FNETRGSIPV
510 520 530 540 550
PKYEEEEEEE EGGISTQQCR PRRPLSSLKY TEDMTPTEQT PRGSMESLAL
560 570 580 590 600
SNATGLSAEG GAKRQEHLSR FSMPDLSKDS GMNVSEKLSN MGTLNSSMQF
610 620 630 640 650
RSAESVRSLL SAQQYQEMEG NLHQLSNPLG YRDLQSHGRM HQSFDFDGGI
660 670 680 690 700
ASSKLPGQEG VHIQPMSERT RRRTTSRDDN RRFRPHRSRR SRRSRSDNAL
710 720 730 740 750
HLASEREVIA RLKERPPLRA REDYDQFMRQ RSFQESLGQG SRRDLYSQCP
760 770 780 790 800
RTVSDLALQN AFGERWGPYF TEYDWCSTCS SSSESDNEGY FLGEPIPQPA
810 820 830 840
RLRYVTSDEL LHKYSSYGVP KSSTLGGRGQ LHSRKRQKSK NCIIS
Length:845
Mass (Da):95,781
Last modified:July 27, 2011 - v3
Checksum:i234C14897AD3F261
GO

Sequence cautioni

The sequence AAH50793.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti273 – 2731G → D in AAH50793 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH466523 Genomic DNA. Translation: EDK99322.1.
BC145754 mRNA. Translation: AAI45755.1.
BC050793 mRNA. Translation: AAH50793.1. Different initiation.
CCDSiCCDS51854.1.
RefSeqiNP_001127931.1. NM_001134459.1.
NP_001127932.1. NM_001134460.2.
NP_001127933.1. NM_001134461.1.
UniGeneiMm.248820.

Genome annotation databases

EnsembliENSMUST00000113445; ENSMUSP00000109072; ENSMUSG00000030020.
ENSMUST00000113446; ENSMUSP00000109073; ENSMUSG00000030020.
ENSMUST00000113447; ENSMUSP00000109074; ENSMUSG00000030020.
GeneIDi243548.
KEGGimmu:243548.
UCSCiuc009cyx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH466523 Genomic DNA. Translation: EDK99322.1.
BC145754 mRNA. Translation: AAI45755.1.
BC050793 mRNA. Translation: AAH50793.1. Different initiation.
CCDSiCCDS51854.1.
RefSeqiNP_001127931.1. NM_001134459.1.
NP_001127932.1. NM_001134460.2.
NP_001127933.1. NM_001134461.1.
UniGeneiMm.248820.

3D structure databases

ProteinModelPortaliQ80Y24.
SMRiQ80Y24. Positions 128-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232537. 1 interaction.
STRINGi10090.ENSMUSP00000032093.

PTM databases

iPTMnetiQ80Y24.
PhosphoSiteiQ80Y24.

Proteomic databases

MaxQBiQ80Y24.
PaxDbiQ80Y24.
PRIDEiQ80Y24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113445; ENSMUSP00000109072; ENSMUSG00000030020.
ENSMUST00000113446; ENSMUSP00000109073; ENSMUSG00000030020.
ENSMUST00000113447; ENSMUSP00000109074; ENSMUSG00000030020.
GeneIDi243548.
KEGGimmu:243548.
UCSCiuc009cyx.2. mouse.

Organism-specific databases

CTDi166336.
MGIiMGI:1925144. Prickle2.

Phylogenomic databases

eggNOGiKOG1704. Eukaryota.
ENOG410XP0W. LUCA.
GeneTreeiENSGT00550000074438.
HOGENOMiHOG000290649.
HOVERGENiHBG053679.
InParanoidiQ80Y24.
KOiK04511.
OrthoDBiEOG7P8P7M.
TreeFamiTF313265.

Miscellaneous databases

ChiTaRSiPrickle2. mouse.
PROiQ80Y24.
SOURCEiSearch...

Gene expression databases

BgeeiQ80Y24.
CleanExiMM_PRICKLE2.
ExpressionAtlasiQ80Y24. baseline and differential.
GenevisibleiQ80Y24. MM.

Family and domain databases

Gene3Di2.10.110.10. 3 hits.
InterProiIPR010442. PET_domain.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 3 hits.
PF06297. PET. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 3 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 3 hits.
PS51303. PET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. "Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle."
    Katoh M., Katoh M.
    Int. J. Mol. Med. 11:249-256(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, CHARACTERIZATION.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319; SER-321; SER-322; THR-535; THR-537; THR-540; SER-544; SER-547; SER-608; SER-643 AND SER-732, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney and Lung.
  6. Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPRIC2_MOUSE
AccessioniPrimary (citable) accession number: Q80Y24
Secondary accession number(s): A6H652
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.