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Protein

Lethal(2) giant larvae protein homolog 1

Gene

Llgl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells. Involved in axonogenesis through RAB10 activation thereby regulating vesicular membrane trafficking toward the axonal plasma membrane.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Lethal(2) giant larvae protein homolog 1
Short name:
LLGL
Alternative name(s):
Mgl-1
Mlgl
Gene namesi
Name:Llgl1
Synonyms:Llglh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:102682. Llgl1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: UniProtKB-SubCell
  • basolateral plasma membrane Source: MGI
  • cortical actin cytoskeleton Source: MGI
  • cytoplasm Source: MGI
  • cytoskeleton Source: UniProtKB
  • early endosome membrane Source: UniProtKB
  • Golgi cis cisterna Source: UniProtKB
  • myelin sheath abaxonal region Source: BHF-UCL
  • plasma membrane Source: GO_Central
  • trans-Golgi network membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice exhibit disorganization and disruption of the apical junctional complex, resulting in hyper-proliferation of neuroblasts and brain dysplasia. Loss of Lgl1 in mice results in formation of neuroepithelial rosette-like structures, similar to the neuroblastic rosettes in human primitive neuroectodermal tumors. The newborn Lgl1(-/-) pups develop severe hydrocephalus and die neonatally. Due to the loss of mitotic spindle orientation, a large proportion of Lgl1(-/-) neural progenitor cells fails to exit the cell cycle and differentiate, and, instead, continues to proliferate and dies by apoptosis. Dividing Lgl1(-/-) cells are unable to asymmetrically localize the Notch inhibitor Numb, and the resulting failure of asymmetric cell divisions may be responsible for the hyperproliferation and the lack of differentiation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Lethal(2) giant larvae protein homolog 1PRO_0000232726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei662 – 6621PhosphoserineBy similarity
Modified residuei957 – 9571PhosphothreonineCombined sources
Modified residuei964 – 9641PhosphoserineCombined sources
Modified residuei982 – 9821PhosphoserineCombined sources
Modified residuei989 – 9891PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated by PRKCI on at least one of the following Ser residues: Ser 654, Ser-658, Ser-662, Ser-669 and Ser-672. Phosphorylation is important for appropriated cell polarization.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ80Y17.
PaxDbiQ80Y17.
PeptideAtlasiQ80Y17.
PRIDEiQ80Y17.

PTM databases

iPTMnetiQ80Y17.
PhosphoSiteiQ80Y17.
SwissPalmiQ80Y17.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020536.
CleanExiMM_LLGL1.
GenevisibleiQ80Y17. MM.

Interactioni

Subunit structurei

Associated with nonmuscle myosin II heavy chain. Interacts with PRKCI/aPKC, PARD6B/Par-6 and PARD6A (By similarity). Interacts with STX4A. Interacts with RAB10 (GDP-bound form); the interaction is direct and promotes RAB10 association with membranes and activation through competition with the Rab inhibitor GDI1. Interacts with VPRBP (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060749.

Structurei

3D structure databases

ProteinModelPortaliQ80Y17.
SMRiQ80Y17. Positions 438-463, 598-631.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati38 – 7134WD 1Add
BLAST
Repeati78 – 11942WD 2Add
BLAST
Repeati139 – 17537WD 3Add
BLAST
Repeati199 – 23335WD 4Add
BLAST
Repeati239 – 27133WD 5Add
BLAST
Repeati289 – 33143WD 6Add
BLAST
Repeati339 – 37335WD 7Add
BLAST
Repeati395 – 47379WD 8Add
BLAST
Repeati517 – 59276WD 9Add
BLAST
Repeati601 – 66262WD 10Add
BLAST
Repeati722 – 78261WD 11Add
BLAST
Repeati791 – 84353WD 12Add
BLAST
Repeati848 – 90154WD 13Add
BLAST
Repeati915 – 93824WD 14Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat L(2)GL family.Curated
Contains 14 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1983. Eukaryota.
ENOG410XS6Z. LUCA.
GeneTreeiENSGT00390000000018.
HOGENOMiHOG000115700.
HOVERGENiHBG052711.
InParanoidiQ80Y17.
KOiK06094.
PhylomeDBiQ80Y17.
TreeFamiTF314585.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
[Graphical view]
PRINTSiPR00962. LETHAL2GIANT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80Y17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKFRFRRQG ADPQREKLKQ ELFAFHKTVE HGFPNQPSAL AFDPELRIMA
60 70 80 90 100
IGTRSGAVKI YGAPGVEFTG LHRDAATVTQ MHFLPGQGRL LTLLDDSSLH
110 120 130 140 150
LWEIIHHNGC AHLEEGLSFH PPSRPSFDNA SFPASLTRVT VVLLVAGNTA
160 170 180 190 200
ALGTESGSIF FLDVATLALL EGQTLSPDVV LRSVPDDYRC GKALGPVESL
210 220 230 240 250
QGHLQDPSKI LIGYSRGLLV IWSQATQSVD NVFLGNQQLE SLCWGRDGSS
260 270 280 290 300
IISSHSDGSY AIWSTDTGSP PTLQPTVVTT PYGPFPCKAI NKILWRSCES
310 320 330 340 350
GDHFIIFSGG MPRASYGDRH CVSVLRAETL VTLDFTSRVI DFFTVHSTQP
360 370 380 390 400
EDECDNPQAL AVLLEEELVV LDLQTPGWPA VPAPYLAPLH SSAITCSAHV
410 420 430 440 450
ANVPSKLWAR IVSAGEQQSP QPASSALSWP ITGGRNLAQE PSQRGLLLTG
460 470 480 490 500
HEDGTVRFWD ASGVALRPLY KLSTAGLFQT DCEHADSLAQ AVEDDWPPFR
510 520 530 540 550
KVGCFDPYSD DPRLGIQKVA LCKYTAQMVV AGTAGQVLVL ELSEVPAEHA
560 570 580 590 600
VSVANVDLLQ DREGFTWKGH ERLNPHTGLL PWPAGFQPRM LIQCLPPAAV
610 620 630 640 650
TAVTLHAEWS LVAFGTSHGF GLFDYQRKSP VLARCTLHPN DSLAMEGPLS
660 670 680 690 700
RVKSLKKSLR QSFRRIRKSR VSGKKRTPAA SSKLQEANAQ LAEQTCPHDL
710 720 730 740 750
EMTPVQRRIE PRSADDSLSG VVRCLYFADT FLRDATHHGP TMWAGTNSGS
760 770 780 790 800
VFAYALEVPA ATAGGEKRPE QAVEAVLGKE VQLMHRAPVV AIAVLDGRGR
810 820 830 840 850
PLPEPYEASR DLAQAPDMQG GHAVLIASEE QFKVFTLPKV SAKTKFKLTA
860 870 880 890 900
HEGCRVRKVA LATFASVMSE DYAETCLACL TNLGDVHVFS VPGLRPQVHY
910 920 930 940 950
SCIRKEDISG IASCVFTRHG QGFYLISPSE FERFSLSARN ITEPLCSLDI
960 970 980 990 1000
SWPQNATQPR LQESPKLSQA NGTRDIILAP ESCEGSPSSA HSKRADTMEP
1010 1020 1030
PEAALSPVSI DSAASGDTML DTTGDVTVEY VKDFLG
Length:1,036
Mass (Da):112,618
Last modified:June 1, 2003 - v1
Checksum:i715EFC11A45CD001
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti321 – 3211C → F in BAC39171 (PubMed:16141072).Curated
Sequence conflicti684 – 6852Missing in BAA03712 (PubMed:8103190).Curated
Sequence conflicti1036 – 10361G → GSPEDSEKNLRNLEADDACR AYTLLIK in AAH55399 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16141 mRNA. Translation: BAA03712.1.
AK084386 mRNA. Translation: BAC39171.1.
AK159412 mRNA. Translation: BAE35062.1.
AL596215 Genomic DNA. Translation: CAI35271.1.
AL596215 Genomic DNA. Translation: CAI35272.1.
BC050913 mRNA. Translation: AAH50913.1.
BC055399 mRNA. Translation: AAH55399.1.
CCDSiCCDS48813.1.
PIRiS36758.
RefSeqiNP_001152876.1. NM_001159404.1.
NP_001152877.1. NM_001159405.1.
NP_032528.1. NM_008502.2.
UniGeneiMm.285453.

Genome annotation databases

EnsembliENSMUST00000108719; ENSMUSP00000104359; ENSMUSG00000020536.
GeneIDi16897.
KEGGimmu:16897.
UCSCiuc007jge.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16141 mRNA. Translation: BAA03712.1.
AK084386 mRNA. Translation: BAC39171.1.
AK159412 mRNA. Translation: BAE35062.1.
AL596215 Genomic DNA. Translation: CAI35271.1.
AL596215 Genomic DNA. Translation: CAI35272.1.
BC050913 mRNA. Translation: AAH50913.1.
BC055399 mRNA. Translation: AAH55399.1.
CCDSiCCDS48813.1.
PIRiS36758.
RefSeqiNP_001152876.1. NM_001159404.1.
NP_001152877.1. NM_001159405.1.
NP_032528.1. NM_008502.2.
UniGeneiMm.285453.

3D structure databases

ProteinModelPortaliQ80Y17.
SMRiQ80Y17. Positions 438-463, 598-631.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060749.

PTM databases

iPTMnetiQ80Y17.
PhosphoSiteiQ80Y17.
SwissPalmiQ80Y17.

Proteomic databases

MaxQBiQ80Y17.
PaxDbiQ80Y17.
PeptideAtlasiQ80Y17.
PRIDEiQ80Y17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108719; ENSMUSP00000104359; ENSMUSG00000020536.
GeneIDi16897.
KEGGimmu:16897.
UCSCiuc007jge.2. mouse.

Organism-specific databases

CTDi3996.
MGIiMGI:102682. Llgl1.

Phylogenomic databases

eggNOGiKOG1983. Eukaryota.
ENOG410XS6Z. LUCA.
GeneTreeiENSGT00390000000018.
HOGENOMiHOG000115700.
HOVERGENiHBG052711.
InParanoidiQ80Y17.
KOiK06094.
PhylomeDBiQ80Y17.
TreeFamiTF314585.

Miscellaneous databases

ChiTaRSiLlgl1. mouse.
PROiQ80Y17.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020536.
CleanExiMM_LLGL1.
GenevisibleiQ80Y17. MM.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
[Graphical view]
PRINTSiPR00962. LETHAL2GIANT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiL2GL1_MOUSE
AccessioniPrimary (citable) accession number: Q80Y17
Secondary accession number(s): Q61856, Q7TNV3, Q8BN92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.