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Protein

TBC1 domain family member 5

Gene

Tbc1d5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May act as a GTPase-activating protein for Rab family protein(s). May act as a GAP for RAB7A. Can displace RAB7A and retromer CSC subcomplex from the endosomal membrane to the cytosol; at least retromer displacement seems to require its catalytic activity. Required for retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN); the function seems to require its catalytic activity. Involved in regulation of autophagy. May act as a molecular switch between endosomal and autophagosomal transport and is involved in reprogramming vesicle trafficking upon autophagy induction. Involved in the trafficking of ATG9A upon activation of autophagy. May regulate the recruitment of ATG9A-AP2-containing vesicles to autophagic membranes (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei169 – 1691Arginine fingerBy similarity
Sitei204 – 2041Glutamine fingerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Autophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
TBC1 domain family member 5
Gene namesi
Name:Tbc1d5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1919488. Tbc1d5.

Subcellular locationi

  • Endosome membrane By similarity
  • Cytoplasmic vesicleautophagosome By similarity

  • Note: During starvation induced autophagy is relocalized from endosomal localization to LC3-positive autophagosomes (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 815815TBC1 domain family member 5PRO_0000208029Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei460 – 4601PhosphoserineBy similarity
Modified residuei546 – 5461PhosphoserineBy similarity
Modified residuei563 – 5631PhosphoserineBy similarity
Modified residuei565 – 5651PhosphoserineCombined sources
Modified residuei568 – 5681PhosphoserineCombined sources
Modified residuei578 – 5781PhosphoserineCombined sources
Modified residuei608 – 6081PhosphoserineCombined sources
Modified residuei811 – 8111PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80XQ2.
MaxQBiQ80XQ2.
PaxDbiQ80XQ2.
PRIDEiQ80XQ2.

PTM databases

iPTMnetiQ80XQ2.
PhosphoSiteiQ80XQ2.

Expressioni

Gene expression databases

BgeeiQ80XQ2.
CleanExiMM_TBC1D5.
GenevisibleiQ80XQ2. MM.

Interactioni

Subunit structurei

Interacts with MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1, GABARAPL2. Interacts with VPS29 and VPS35; indicative for an association with retromer CSC subcomplex. MAP1LC3A and VPS29 compete for binding to TBC1D5. Interacts with AP2M1; indicative for an association with the AP2 complex. Interacts with ULK1 and ATG13 (phosphorylated); indicative for an association with the activated ULK1-ATG13-FIP200 complex. Interacts with ATG9A; the interactions seems to be restricted to the AP2-clathrin-associated fraction of ATG9A.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024717.

Structurei

3D structure databases

ProteinModelPortaliQ80XQ2.
SMRiQ80XQ2. Positions 163-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 359279Rab-GAP TBCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni56 – 649Required for interaction with retromer; involved in interaction with ATG8 family proteinsBy similarity
Regioni806 – 8116Required for interaction with ATG8 family proteinsBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 626LIR 1By similarity
Motifi805 – 8095LIR 2By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi510 – 53930Gln-richAdd
BLAST

Domaini

The arginine and glutamine fingers are critical for the GTPase-activating mechanism, they pull out Rab's 'switch 2' glutamine and insert in Rab's active site.By similarity
The LIR (LC3-interacting region) motif mediates the interaction with ATG8 family proteins. LIR 1 is also implicated in interaction with retromer; LIR 2 is only implicated in interaction with ATG8 family proteins.By similarity

Sequence similaritiesi

Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1091. Eukaryota.
ENOG410YX8Z. LUCA.
GeneTreeiENSGT00840000129860.
HOVERGENiHBG062913.
InParanoidiQ80XQ2.
KOiK18469.
OMAiNFQTCLG.
OrthoDBiEOG7NGQC2.
TreeFamiTF105784.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 3 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80XQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKSVSETRH PLQSEEQEVG IDPLFSYSNK TRGDLSQNGR GSNSTLDTEG
60 70 80 90 100
TFNSYMKEWE ELFVNNNYLA TVRQKGINGQ LRSSRFRSIC WKLFLCVLPQ
110 120 130 140 150
DKSQWISKIK ELRAWYSSIK EIHITNPRKA AGQQDLMINN PLSQDEGSLW
160 170 180 190 200
NKFFQDKELR SMIEQDVKRT FPEMQFFQQE NVRKILTDVL FCYARENEQL
210 220 230 240 250
LYKQGMHELL APIIFTLHCD HQAFLHASES AQPSEEMKTL LNPEYLEHDA
260 270 280 290 300
YAMFSQLMET AEPWFSTFEH DGQKGKETLM APIPFARPQD LGPTVAIVTK
310 320 330 340 350
VNQIQDHLLK KHDIELYMHL NRLEIAPQIY GLRWVRLLFG REFPLQDLLV
360 370 380 390 400
VWDALFADSL NLSLVDYVFT AMLLYIRDAL ISSNYQTCLG LLMHYPIIGD
410 420 430 440 450
IHSLILKALF LRDPKRNPRP ATYQFHPNLD YYKARGADLM NKSRTNARGA
460 470 480 490 500
PLNIHKVSNS LINFGRKLIS PASAPGSMGG PVPGNNSSSS FSAAIPTRTS
510 520 530 540 550
TEAPRHHLLQ QQQQQQHQQQ QQQQPQQQQQ QHQQQQQQQR LMKSESMPVQ
560 570 580 590 600
LNKGQSSKTI SSSPSIESLP GGREFTGSPP PSATKKDSFF SNIARSRSHS
610 620 630 640 650
KTMGRKESEE ELEAQISFLQ GQLNDLDAMC KYCAKVMDMH LVNIQDVVLQ
660 670 680 690 700
ENLEKEDQIL VSLAGLKQIK DILKGSLRFN QSQLEAGENE QITIADDHYC
710 720 730 740 750
SSGQDQGSQV PRAAKQASSE MPGCTGGTTP DDFILVSKED EGHRARGAFS
760 770 780 790 800
GQAQPLLTLR STSGKSRAPA CSPLLFSDPL MGPASASASS SNPSSSPDDD
810
SSKESGFTIV SPLDI
Length:815
Mass (Da):91,837
Last modified:July 27, 2011 - v2
Checksum:i62E6D23B36398E9F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti770 – 7701A → S in AAH43113 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC099694 Genomic DNA. No translation available.
AC110168 Genomic DNA. No translation available.
AC125140 Genomic DNA. No translation available.
AC154214 Genomic DNA. No translation available.
CT025541 Genomic DNA. No translation available.
BC043113 mRNA. Translation: AAH43113.1.
CCDSiCCDS37650.1.
RefSeqiNP_001272922.1. NM_001285993.1.
NP_082438.3. NM_028162.4.
UniGeneiMm.120650.

Genome annotation databases

EnsembliENSMUST00000024717; ENSMUSP00000024717; ENSMUSG00000023923.
GeneIDi72238.
KEGGimmu:72238.
UCSCiuc008cyy.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC099694 Genomic DNA. No translation available.
AC110168 Genomic DNA. No translation available.
AC125140 Genomic DNA. No translation available.
AC154214 Genomic DNA. No translation available.
CT025541 Genomic DNA. No translation available.
BC043113 mRNA. Translation: AAH43113.1.
CCDSiCCDS37650.1.
RefSeqiNP_001272922.1. NM_001285993.1.
NP_082438.3. NM_028162.4.
UniGeneiMm.120650.

3D structure databases

ProteinModelPortaliQ80XQ2.
SMRiQ80XQ2. Positions 163-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024717.

PTM databases

iPTMnetiQ80XQ2.
PhosphoSiteiQ80XQ2.

Proteomic databases

EPDiQ80XQ2.
MaxQBiQ80XQ2.
PaxDbiQ80XQ2.
PRIDEiQ80XQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024717; ENSMUSP00000024717; ENSMUSG00000023923.
GeneIDi72238.
KEGGimmu:72238.
UCSCiuc008cyy.3. mouse.

Organism-specific databases

CTDi9779.
MGIiMGI:1919488. Tbc1d5.

Phylogenomic databases

eggNOGiKOG1091. Eukaryota.
ENOG410YX8Z. LUCA.
GeneTreeiENSGT00840000129860.
HOVERGENiHBG062913.
InParanoidiQ80XQ2.
KOiK18469.
OMAiNFQTCLG.
OrthoDBiEOG7NGQC2.
TreeFamiTF105784.

Miscellaneous databases

ChiTaRSiTbc1d5. mouse.
PROiQ80XQ2.
SOURCEiSearch...

Gene expression databases

BgeeiQ80XQ2.
CleanExiMM_TBC1D5.
GenevisibleiQ80XQ2. MM.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 3 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-565 AND SER-568, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-578, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-565; SER-568 AND SER-608, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiTBCD5_MOUSE
AccessioniPrimary (citable) accession number: Q80XQ2
Secondary accession number(s): E9PZJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.