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Q80XP9

- WNK3_MOUSE

UniProt

Q80XP9 - WNK3_MOUSE

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Protein

Serine/threonine-protein kinase WNK3

Gene
Wnk3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A1, SLC12A2, SLC12A3, SCNN1A, SCNN1B, SCNN1D and SGK1. Inhibits SLC12A4, SLC12A5, SLC12A6 and SLC12A7. Phosphorylates WNK4. Increases Ca2+ influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activation requires autophosphorylation of Ser-307. Phosphorylation of Ser-303 also promotes increased activity. Kinase activity is inhibited by WNK4 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei158 – 1581ATP By similarity
Active sitei274 – 2741Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi152 – 1609ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein kinase activity Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. positive regulation of establishment of protein localization to plasma membrane Source: UniProtKB
  2. positive regulation of ion transmembrane transporter activity Source: UniProtKB
  3. positive regulation of peptidyl-threonine phosphorylation Source: UniProtKB
  4. positive regulation of rubidium ion transmembrane transporter activity Source: UniProtKB
  5. positive regulation of rubidium ion transport Source: UniProtKB
  6. positive regulation of sodium ion transmembrane transporter activity Source: UniProtKB
  7. positive regulation of sodium ion transport Source: UniProtKB
  8. protein autophosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase WNK3 (EC:2.7.11.1)
Alternative name(s):
Protein kinase lysine-deficient 3
Protein kinase with no lysine 3
Gene namesi
Name:Wnk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:2652875. Wnk3.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. adherens junction Source: BHF-UCL
  2. cytoplasm Source: UniProtKB-SubCell
  3. tight junction Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17571757Serine/threonine-protein kinase WNK3PRO_0000278775Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621Phosphoserine By similarity
Modified residuei303 – 3031Phosphoserine; by autocatalysis By similarity
Modified residuei307 – 3071Phosphoserine; by autocatalysis By similarity

Post-translational modificationi

Ubiquitinated by the BCR(KLHL2) complex, leading to its degradation By similarity.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ80XP9.
PRIDEiQ80XP9.

PTM databases

PhosphoSiteiQ80XP9.

Expressioni

Gene expression databases

CleanExiMM_WNK3.
GenevestigatoriQ80XP9.

Interactioni

Subunit structurei

Interacts with WNK1 and WNK4 By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000094005.

Structurei

3D structure databases

ProteinModelPortaliQ80XP9.
SMRiQ80XP9. Positions 135-497.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini146 – 404259Protein kinaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074385.
HOVERGENiHBG050346.
InParanoidiQ80XP9.
KOiK08867.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q80XP9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MATDSGEPAS TEDSEKPDGV SFENRAARAV APLTVEARIK EKYSTFSASG     50
ENIERKRFFR KSVEMTEDDK VAESSRRDER KAATNISRVD KVPTNVLRGG 100
QEVKYEQCSK ATSESSKDCF KEKTEKEMEE EAEMKAVATS PSGRFLKFDI 150
ELGRGAFKTV YKGLDTETWV EVAWCELQDR KLTKAEQQRF KEEAEMLKGL 200
QHPNIVRFYD SWESTLKGKK CIVLVTELMT SGTLKTYLKR FKVMKPKVLR 250
SWCRQILKGL QFLHTRTPPI IHRDLKCDNI FITGPTGSVK IGDLGLATLM 300
RTSFAKSVIG TPEFMAPEMY EEHYDESVDV YAFGMCMLEM ATSEYPYSEC 350
QNAAQIYRKV TSGIKPASFN KVTDPEVKEI IEGCIRQNKS ERLSIKDLLN 400
HAFFAEDTGL RVELAEEDDC SNSSLALRLW VEDPKKLKGK HKDNEAIEFS 450
FNLEADTPEE VAYEMVKSGF FHESDSKAVA KSIRDRVTLI KKIREKKPAG 500
CLEERRDSQC KYVRNVLPQQ QTATLQPTPG PHTAAEYEET EVDQHVRQQF 550
LQGKPQQQSS SVRGDTSSEP TAGPVLHSDT SSHPTVAYSS NQTTSSQEQP 600
KLTQSPVLPV VQGQSSVMPI YAAGVGVVSQ SQISPLTIQK VSQIKPVSQP 650
IGAEQQATLQ NPDFVRSLNQ DVTSVKENTN NPDTPSGNGK QDRNKQRRAS 700
CPRPEKGTKF QLTVLQVSVS GDNMVECQLE THNNKMVTFK FDVDGDAPED 750
IADYMVEDNF VLENEKEKFV EELRAIVGQA QEILHVHSAV EKSIGVDSVA 800
LESNSNQTGS SEQVLINSAS TQTSNESAPQ SSPVGRWRFC INQTIKNREA 850
QSPPSLQPSM AMVPGLHPFP SSRNTSNQAI SQNTVFTIEN NPGHRELFTS 900
KLDHKDVVDG KIGEHASIET EQSSISYQVE DDRQIMTPAT DNSNYSAALV 950
CPVPGECEAL TSQAGMFMPT YPNQQAAVLA DVHIAYPGES VPIGGNAALT 1000
SVLVSSDQKP QSLSVQQPTI DAEFISKEGE TTVNTETSSP KAVIATQTPG 1050
FEPAVILPAT ILESDGERPP KMEFADNRIK TLDEKLRNLL YQEHSISSIC 1100
PESQKDTQSI DSPFSSSAED ILSYSMPEVI AISHCGIQDS PAQSPNFQQT 1150
GSKILSNVAA SQPAHISVFK KDLNVITSVP SELCLHEMSP DASLPGDPEA 1200
YPAAVSSDGT IHLQTGGGYF GLSFTCPSLK NPISRKSWTR KLKSWAYRLR 1250
QSTSFFKRSK VRQVETEDKR SAIASDPIPL TREFSSDTRA LSRCKAMSGS 1300
FQRGRFQVIT VPQQQPVKMM SFGKDHRPPF NKTTVQSSEQ ALTFAEAAVS 1350
QLIEVEPAMP THKASVSSRK LRTLYETFKE DKGDPEQGDI VSYSTACETS 1400
VSSVATEKNV TSTTEVSVQS GSEPLDKEKN ESTPGKQTCT NEFSATLAGN 1450
RKSVTKTRPE GDQYLPLREE QAYAQTQNSL FYSPSSPMSS DNESEIEDED 1500
LKVELQRLRE KHIQEVVSLQ TQQNKELQEL YERLRATKDN KAQSSEVPLS 1550
PASPRRPRSF KSKLRSRPQS MTHSDNLVVK DALGVESNTV SCQQSPASKK 1600
GMFTDDLHKL VDDWTRETVG HFPSKPSLNQ LKQSQQKSEA ENWNKSCEST 1650
PSTMGYTSNW ISSLSQIRGA APTSLPQGLP LPSFHGPLAS YGMPHVCQYN 1700
AVGAAGYPVQ WVGISGPAQQ SVVLPTQSGG LFQPGMNLQS FPAPPVQNPA 1750
SIPPGPK 1757
Length:1,757
Mass (Da):193,991
Last modified:January 9, 2013 - v3
Checksum:i3E5B070772BE6725
GO
Isoform 2 (identifier: Q80XP9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1217-1263: Missing.

Note: No experimental confirmation available.

Show »
Length:1,710
Mass (Da):188,423
Checksum:iD825CA0153EC71F6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1217 – 126347Missing in isoform 2. VSP_041934Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JQ247189 mRNA. Translation: AEZ01402.1.
JQ247190 mRNA. Translation: AEZ01403.1.
AL732420 Genomic DNA. No translation available.
AL807396 Genomic DNA. No translation available.
BC043119 mRNA. Translation: AAH43119.1.
BC060731 mRNA. Translation: AAH60731.1.
CCDSiCCDS72450.1. [Q80XP9-2]
CCDS72451.1. [Q80XP9-1]
RefSeqiNP_001258607.1. NM_001271678.1. [Q80XP9-1]
NP_001258608.1. NM_001271679.1. [Q80XP9-2]
XP_006528933.1. XM_006528870.1. [Q80XP9-1]
XP_006528934.1. XM_006528871.1. [Q80XP9-1]
XP_006528935.1. XM_006528872.1. [Q80XP9-1]
XP_006528936.1. XM_006528873.1. [Q80XP9-1]
XP_006528937.1. XM_006528874.1. [Q80XP9-2]
UniGeneiMm.441474.

Genome annotation databases

EnsembliENSMUST00000184392; ENSMUSP00000139037; ENSMUSG00000041245. [Q80XP9-1]
ENSMUST00000184730; ENSMUSP00000138822; ENSMUSG00000041245. [Q80XP9-2]
GeneIDi279561.
KEGGimmu:279561.
UCSCiuc009upa.2. mouse. [Q80XP9-2]
uc029xnn.1. mouse. [Q80XP9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
JQ247189 mRNA. Translation: AEZ01402.1 .
JQ247190 mRNA. Translation: AEZ01403.1 .
AL732420 Genomic DNA. No translation available.
AL807396 Genomic DNA. No translation available.
BC043119 mRNA. Translation: AAH43119.1 .
BC060731 mRNA. Translation: AAH60731.1 .
CCDSi CCDS72450.1. [Q80XP9-2 ]
CCDS72451.1. [Q80XP9-1 ]
RefSeqi NP_001258607.1. NM_001271678.1. [Q80XP9-1 ]
NP_001258608.1. NM_001271679.1. [Q80XP9-2 ]
XP_006528933.1. XM_006528870.1. [Q80XP9-1 ]
XP_006528934.1. XM_006528871.1. [Q80XP9-1 ]
XP_006528935.1. XM_006528872.1. [Q80XP9-1 ]
XP_006528936.1. XM_006528873.1. [Q80XP9-1 ]
XP_006528937.1. XM_006528874.1. [Q80XP9-2 ]
UniGenei Mm.441474.

3D structure databases

ProteinModelPortali Q80XP9.
SMRi Q80XP9. Positions 135-497.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000094005.

PTM databases

PhosphoSitei Q80XP9.

Proteomic databases

PaxDbi Q80XP9.
PRIDEi Q80XP9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000184392 ; ENSMUSP00000139037 ; ENSMUSG00000041245 . [Q80XP9-1 ]
ENSMUST00000184730 ; ENSMUSP00000138822 ; ENSMUSG00000041245 . [Q80XP9-2 ]
GeneIDi 279561.
KEGGi mmu:279561.
UCSCi uc009upa.2. mouse. [Q80XP9-2 ]
uc029xnn.1. mouse. [Q80XP9-1 ]

Organism-specific databases

CTDi 65267.
MGIi MGI:2652875. Wnk3.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074385.
HOVERGENi HBG050346.
InParanoidi Q80XP9.
KOi K08867.

Miscellaneous databases

NextBioi 35562242.
PROi Q80XP9.
SOURCEi Search...

Gene expression databases

CleanExi MM_WNK3.
Genevestigatori Q80XP9.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR024678. Kinase_OSR1/WNK_CCT.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF12202. OSR1_C. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 2 hits.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse Wnk3 gene encodes multiple continuous open reading frames."
    Shmukler B.E., Alper S.L.
    Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1296-1757.
    Strain: C57BL/6.
    Tissue: Embryonic brain.
  4. "Renal and brain isoforms of WNK3 have opposite effects on NCCT expression."
    Glover M., Zuber A.M., O'Shaughnessy K.M.
    J. Am. Soc. Nephrol. 20:1314-1322(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ALTERNATIVE SPLICING.

Entry informationi

Entry nameiWNK3_MOUSE
AccessioniPrimary (citable) accession number: Q80XP9
Secondary accession number(s): H6WJI2, H6WJI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: January 9, 2013
Last modified: July 9, 2014
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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