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Protein

D-beta-hydroxybutyrate dehydrogenase, mitochondrial

Gene

Bdh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(R)-3-hydroxybutanoate + NAD+ = acetoacetate + NADH.By similarity

Enzyme regulationi

Requires phosphatidylcholine as an allosteric activator for enzymatic activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei196SubstrateBy similarity1
Active sitei209Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi60 – 84NADBy similarityAdd BLAST25

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-MMU-77108. Utilization of Ketone Bodies.
R-MMU-77111. Synthesis of Ketone Bodies.

Names & Taxonomyi

Protein namesi
Recommended name:
D-beta-hydroxybutyrate dehydrogenase, mitochondrialCurated (EC:1.1.1.30By similarity)
Alternative name(s):
3-hydroxybutyrate dehydrogenaseBy similarity
Short name:
BDHBy similarity
Gene namesi
Name:Bdh1Imported
Synonyms:BdhBy similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1919161. Bdh1.

Subcellular locationi

  • Mitochondrion inner membrane By similarity
  • Mitochondrion matrix By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • matrix side of mitochondrial inner membrane Source: UniProtKB
  • mitochondrial inner membrane Source: MGI
  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 46MitochondrionBy similarityAdd BLAST46
ChainiPRO_000003196147 – 343D-beta-hydroxybutyrate dehydrogenase, mitochondrialAdd BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73N6-acetyllysineCombined sources1
Modified residuei97N6-acetyllysineCombined sources1
Modified residuei103N6-acetyllysine; alternateCombined sources1
Modified residuei103N6-succinyllysine; alternateCombined sources1
Modified residuei132N6-acetyllysineCombined sources1
Modified residuei177N6-acetyllysineCombined sources1
Modified residuei212N6-acetyllysineCombined sources1
Glycosylationi219O-linked (GlcNAc)By similarity1
Modified residuei246PhosphoserineBy similarity1
Modified residuei258N6-acetyllysineCombined sources1
Modified residuei259N6-acetyllysine; alternateCombined sources1
Modified residuei259N6-succinyllysine; alternateCombined sources1
Modified residuei280N6-acetyllysineCombined sources1

Post-translational modificationi

Acetylation of Lys-132 is observed in liver mitochondria from fasted mice but not from fed mice.

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ80XN0.
MaxQBiQ80XN0.
PaxDbiQ80XN0.
PeptideAtlasiQ80XN0.
PRIDEiQ80XN0.

PTM databases

iPTMnetiQ80XN0.
PhosphoSitePlusiQ80XN0.
SwissPalmiQ80XN0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000046598.
CleanExiMM_BDH1.
ExpressionAtlasiQ80XN0. baseline and differential.
GenevisibleiQ80XN0. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ80XN0. 6 interactors.
MINTiMINT-1861188.
STRINGi10090.ENSMUSP00000087192.

Structurei

3D structure databases

ProteinModelPortaliQ80XN0.
SMRiQ80XN0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1610. Eukaryota.
ENOG410Y7FK. LUCA.
GeneTreeiENSGT00860000133667.
HOVERGENiHBG005482.
InParanoidiQ80XN0.
KOiK00019.
OrthoDBiEOG091G0LMI.
TreeFamiTF325617.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80XN0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAARLSRPL SQLPGKALSV RDRENGTRHT LLFYPASFSP DTRRTYASQA
60 70 80 90 100
DAASGKAILI TGCDSGFGFS LAKHLHSKGF LVFAGCLMKD KGDAGVKELD
110 120 130 140 150
SLKSDRLRTI QLNVCNSEEV EKAVETIRSG LKDPEKGMWG LVNNAGISTF
160 170 180 190 200
GEVEFTSMET YKEVAEVNLW GTVRTTKSFL PLLRRAKGRV VNISSMLGRM
210 220 230 240 250
ANPARSPYCI TKFGIEAFSD CLRYEMHPLG VKVSVVEPGN FIAATSLYSP
260 270 280 290 300
ERIQAIAKKM WDDLPEVVRK DYGRKYFDEK IAKMETYCNS GSTDTSSVIN
310 320 330 340
AVTHALTAAT PYTRYHPMDY YWWLRMQIMT HFPGAISDKI YIH
Length:343
Mass (Da):38,299
Last modified:July 27, 2011 - v2
Checksum:iB72B0550713EDB7F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152E → G in BAC36453 (PubMed:16141072).Curated1
Sequence conflicti215I → V in BAC36453 (PubMed:16141072).Curated1
Sequence conflicti215I → V in BAE23523 (PubMed:16141072).Curated1
Sequence conflicti215I → V in BAE26605 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076718 mRNA. Translation: BAC36453.1.
AK137955 mRNA. Translation: BAE23523.1.
AK145711 mRNA. Translation: BAE26605.1.
AK146321 mRNA. Translation: BAE27076.1.
BC027063 mRNA. Translation: AAH27063.1.
BC043683 mRNA. Translation: AAH43683.1.
BC096457 mRNA. Translation: AAH96457.1.
CCDSiCCDS28106.1.
RefSeqiNP_001116155.1. NM_001122683.1.
NP_780386.3. NM_175177.4.
UniGeneiMm.293470.

Genome annotation databases

EnsembliENSMUST00000089759; ENSMUSP00000087192; ENSMUSG00000046598.
ENSMUST00000115226; ENSMUSP00000110881; ENSMUSG00000046598.
ENSMUST00000115227; ENSMUSP00000110882; ENSMUSG00000046598.
GeneIDi71911.
KEGGimmu:71911.
UCSCiuc007yxi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076718 mRNA. Translation: BAC36453.1.
AK137955 mRNA. Translation: BAE23523.1.
AK145711 mRNA. Translation: BAE26605.1.
AK146321 mRNA. Translation: BAE27076.1.
BC027063 mRNA. Translation: AAH27063.1.
BC043683 mRNA. Translation: AAH43683.1.
BC096457 mRNA. Translation: AAH96457.1.
CCDSiCCDS28106.1.
RefSeqiNP_001116155.1. NM_001122683.1.
NP_780386.3. NM_175177.4.
UniGeneiMm.293470.

3D structure databases

ProteinModelPortaliQ80XN0.
SMRiQ80XN0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80XN0. 6 interactors.
MINTiMINT-1861188.
STRINGi10090.ENSMUSP00000087192.

PTM databases

iPTMnetiQ80XN0.
PhosphoSitePlusiQ80XN0.
SwissPalmiQ80XN0.

Proteomic databases

EPDiQ80XN0.
MaxQBiQ80XN0.
PaxDbiQ80XN0.
PeptideAtlasiQ80XN0.
PRIDEiQ80XN0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089759; ENSMUSP00000087192; ENSMUSG00000046598.
ENSMUST00000115226; ENSMUSP00000110881; ENSMUSG00000046598.
ENSMUST00000115227; ENSMUSP00000110882; ENSMUSG00000046598.
GeneIDi71911.
KEGGimmu:71911.
UCSCiuc007yxi.2. mouse.

Organism-specific databases

CTDi622.
MGIiMGI:1919161. Bdh1.

Phylogenomic databases

eggNOGiKOG1610. Eukaryota.
ENOG410Y7FK. LUCA.
GeneTreeiENSGT00860000133667.
HOVERGENiHBG005482.
InParanoidiQ80XN0.
KOiK00019.
OrthoDBiEOG091G0LMI.
TreeFamiTF325617.

Enzyme and pathway databases

ReactomeiR-MMU-77108. Utilization of Ketone Bodies.
R-MMU-77111. Synthesis of Ketone Bodies.

Miscellaneous databases

ChiTaRSiBdh1. mouse.
PROiQ80XN0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046598.
CleanExiMM_BDH1.
ExpressionAtlasiQ80XN0. baseline and differential.
GenevisibleiQ80XN0. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBDH_MOUSE
AccessioniPrimary (citable) accession number: Q80XN0
Secondary accession number(s): Q3UJS9
, Q3UL45, Q8BK53, Q8R0C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.