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Protein

Metallophosphoesterase 1

Gene

Mppe1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Metallophosphoesterase required for transport of GPI-anchor proteins from the endoplasmic reticulum to the Golgi. Acts in lipid remodeling steps of GPI-anchor maturation by mediating the removal of a side-chain ethanolamine-phosphate (EtNP) from the second Man (Man2) of the GPI intermediate, an essential step for efficient transport of GPI-anchor proteins (By similarity).By similarity

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi75 – 751Divalent metal cation 2By similarity
Metal bindingi117 – 1171Divalent metal cation 1By similarity
Metal bindingi117 – 1171Divalent metal cation 2By similarity
Metal bindingi155 – 1551Divalent metal cation 1By similarity
Metal bindingi249 – 2491Divalent metal cation 1By similarity
Metal bindingi303 – 3031Divalent metal cation 1By similarity
Metal bindingi305 – 3051Divalent metal cation 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

ER-Golgi transport, GPI-anchor biosynthesis, Transport

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Metallophosphoesterase 1 (EC:3.1.-.-)
Alternative name(s):
Post-GPI attachment to proteins factor 5
Gene namesi
Name:Mppe1
Synonyms:Pgap5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2661311. Mppe1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei28 – 4821HelicalSequence analysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Metallophosphoesterase 1PRO_0000315729Add
BLAST

Proteomic databases

EPDiQ80XL7.
MaxQBiQ80XL7.
PaxDbiQ80XL7.
PRIDEiQ80XL7.

PTM databases

iPTMnetiQ80XL7.
PhosphoSiteiQ80XL7.

Expressioni

Gene expression databases

BgeeiQ80XL7.
CleanExiMM_MPPE1.

Interactioni

Subunit structurei

Interacts with GPI-anchor proteins. Interacts with TMED10 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072808.

Structurei

3D structure databases

ProteinModelPortaliQ80XL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3662. Eukaryota.
ENOG410ZM57. LUCA.
GeneTreeiENSGT00390000013236.
HOGENOMiHOG000006790.
HOVERGENiHBG101555.
InParanoidiQ80XL7.
OMAiHYPLYRA.
OrthoDBiEOG722J8P.
PhylomeDBiQ80XL7.
TreeFamiTF314437.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR033308. PGAP5/Cdc1/Ted1.
[Graphical view]
PANTHERiPTHR13315. PTHR13315. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80XL7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVRWGLKR QNFHPLRRRR RALLLKLTVV IISVLLFCEY FIYYLVLFRC
60 70 80 90 100
HWPEVKTLAH GGRQEPVLKA MFLADTHLLG EIRGHWLDKL RREWQMERAF
110 120 130 140 150
QTALWLLQPE VVFILGDIFD EGKWSSDQAW ADDVQRFQRM FRHDSHVQLK
160 170 180 190 200
VVIGNHDVGF HYQMSKYRIK RFEKVFGSER LLSLKGVNFV MVNSVAMEGD
210 220 230 240 250
GCIICSEEEA ELREISRKLN CSQEVPGSSQ CDREPEPRLP LSAPVLLQHY
260 270 280 290 300
PLYRASDANC SGEDAAPPEE RNVPFEEKYD VLSREASQKL LWWLRPRLVL
310 320 330 340 350
SGHTHSACEV LHPGGAPEVS VPSFSWRNRN NPSFIMGSLT SRDYALSKCY
360 370 380 390
LPFEDTVLTM YGAAAGFLMI LILVHFEHLP SPFLCGWKLC RLHMRR
Length:396
Mass (Da):46,012
Last modified:June 1, 2003 - v1
Checksum:i8C246D289E05350B
GO
Isoform 2 (identifier: Q80XL7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-224: E → EQ

Show »
Length:397
Mass (Da):46,140
Checksum:iE899D0F159A7452F
GO
Isoform 3 (identifier: Q80XL7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     188-188: N → K
     189-396: Missing.

Note: No experimental confirmation available.
Show »
Length:188
Mass (Da):22,593
Checksum:iA484CC09530F005D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti384 – 3841L → P in BAE32476 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei188 – 1881N → K in isoform 3. 1 PublicationVSP_030684
Alternative sequencei189 – 396208Missing in isoform 3. 1 PublicationVSP_030685Add
BLAST
Alternative sequencei224 – 2241E → EQ in isoform 2. 1 PublicationVSP_030686

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041262 mRNA. Translation: BAC30883.1.
AK154265 mRNA. Translation: BAE32476.1.
AK158446 mRNA. Translation: BAE34513.1.
AK172496 mRNA. Translation: BAE43034.1.
BC045146 mRNA. Translation: AAH45146.1.
BC086456 mRNA. Translation: AAH86456.1.
CCDSiCCDS29319.1. [Q80XL7-2]
RefSeqiNP_766218.1. NM_172630.2. [Q80XL7-2]
XP_006525953.1. XM_006525890.1.
UniGeneiMm.235792.

Genome annotation databases

EnsembliENSMUST00000073054; ENSMUSP00000072808; ENSMUSG00000062526. [Q80XL7-2]
GeneIDi225651.
KEGGimmu:225651.
UCSCiuc008flv.1. mouse. [Q80XL7-2]
uc008flw.1. mouse. [Q80XL7-1]
uc008flx.1. mouse. [Q80XL7-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041262 mRNA. Translation: BAC30883.1.
AK154265 mRNA. Translation: BAE32476.1.
AK158446 mRNA. Translation: BAE34513.1.
AK172496 mRNA. Translation: BAE43034.1.
BC045146 mRNA. Translation: AAH45146.1.
BC086456 mRNA. Translation: AAH86456.1.
CCDSiCCDS29319.1. [Q80XL7-2]
RefSeqiNP_766218.1. NM_172630.2. [Q80XL7-2]
XP_006525953.1. XM_006525890.1.
UniGeneiMm.235792.

3D structure databases

ProteinModelPortaliQ80XL7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072808.

PTM databases

iPTMnetiQ80XL7.
PhosphoSiteiQ80XL7.

Proteomic databases

EPDiQ80XL7.
MaxQBiQ80XL7.
PaxDbiQ80XL7.
PRIDEiQ80XL7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073054; ENSMUSP00000072808; ENSMUSG00000062526. [Q80XL7-2]
GeneIDi225651.
KEGGimmu:225651.
UCSCiuc008flv.1. mouse. [Q80XL7-2]
uc008flw.1. mouse. [Q80XL7-1]
uc008flx.1. mouse. [Q80XL7-3]

Organism-specific databases

CTDi65258.
MGIiMGI:2661311. Mppe1.

Phylogenomic databases

eggNOGiKOG3662. Eukaryota.
ENOG410ZM57. LUCA.
GeneTreeiENSGT00390000013236.
HOGENOMiHOG000006790.
HOVERGENiHBG101555.
InParanoidiQ80XL7.
OMAiHYPLYRA.
OrthoDBiEOG722J8P.
PhylomeDBiQ80XL7.
TreeFamiTF314437.

Miscellaneous databases

PROiQ80XL7.
SOURCEiSearch...

Gene expression databases

BgeeiQ80XL7.
CleanExiMM_MPPE1.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR033308. PGAP5/Cdc1/Ted1.
[Graphical view]
PANTHERiPTHR13315. PTHR13315. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-237 (ISOFORM 1).
    Strain: C57BL/6J and NOD.
    Tissue: Aorta, Inner ear, Spleen and Vein.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor and Olfactory epithelium.

Entry informationi

Entry nameiMPPE1_MOUSE
AccessioniPrimary (citable) accession number: Q80XL7
Secondary accession number(s): Q3T9I6
, Q3TYP9, Q3U4F5, Q8BLU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.