Q80XL1 (CBLC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal transduction protein CBL-C

Alternative name(s):
SH3-binding protein CBL-3
SH3-binding protein CBL-C

Gene names

Name:	Cblc
Synonyms:	Cbl3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulator of EGFR mediated signal transduction By similarity.
Subunit structure	Interacts with EGFR (tyrosine phosphorylated). Interacts with a restricted range of SH3 domain proteins By similarity.
Subcellular location	Nucleus Potential.
Tissue specificity	Ubiquitously expressed. Ref.1
Developmental stage	Expressed at E14.5 in liver, lung and brain. Ref.1
Domain	The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain By similarity. The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme By similarity.
Post-translational modification	Phosphorylated on tyrosines by EGFR By similarity.
Miscellaneous	This protein has one functional calcium-binding site By similarity.
Sequence similarities	Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Calcium Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell surface receptor signaling pathway Inferred from electronic annotation. Source: InterPro negative regulation of MAP kinase activity Inferred from electronic annotation. Source: Compara negative regulation of epidermal growth factor-activated receptor activity Inferred from electronic annotation. Source: Compara protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: Compara ubiquitin-dependent protein catabolic process Inferred from sequence orthology Ref.1. Source: MGI
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell ubiquitin ligase complex Inferred from sequence orthology Ref.1. Source: MGI
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro signal transducer activity Inferred from electronic annotation. Source: InterPro ubiquitin-protein ligase activity Inferred from sequence orthology Ref.1. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q80XL1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q80XL1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     259-259: S → R
     260-496: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Signal transduction protein CBL-C		PRO_0000055867
Regions
	Domain	7 – 320	314	Cbl-PTB
	Calcium binding	196 – 209	14	By similarity
	Zinc finger	350 – 389	40	RING-type
	Region	7 – 144	138	4H
	Region	145 – 217	73	EF-hand-like
	Region	218 – 320	103	SH2-like
	Region	321 – 349	29	Linker By similarity
	Motif	88 – 91	4	Nuclear localization signal By similarity
Sites
	Binding site	263	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	259	1	S → R in isoform 2.		VSP_014946
	Alternative sequence	260 – 496	237	Missing in isoform 2.		VSP_014947
Experimental info
	Sequence conflict	8	1	R → Q in AAK01405. Ref.1
	Sequence conflict	371	1	S → G in AAK01405. Ref.1
	Sequence conflict	375	1	A → S in BAB26782. Ref.2
	Sequence conflict	416	1	G → D in AAH46337. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 4DBE4A14C3D1BF57	FASTA	496	55,715
	10 20 30 40 50 60 MAAAAAPRGW QRGEPRALSR AVKLLQRLEE QCRDPRMVTG PPSLRDLLPR TAQLLGEVAK 70 80 90 100 110 120 ARREAREDPE GPGGADDFLA IYLANLEVKG RQVAELLPPR GKKDVNQDVF REGSRFRRQL 130 140 150 160 170 180 AKLALIFSHM HAELSALFPA GKYCGHLYQL TKGSAHIFWR QNCGVRCVLP WAEFQSLLCA 190 200 210 220 230 240 CHPVEPGPTM QALRSTLDLT CNGHVSVFEF DVFTRLFQPW PTLLRNWQLL AVNHPGYMAF 250 260 270 280 290 300 LTYDEVQTRL QAYRDKPGSY IFRPSCTRLG QWAIGYVSSD GSILQTIPLN KPLLQVLLKG 310 320 330 340 350 360 QKDGIFLFPD GKKHNPDLTE LCRVEPYQRI QVSEEQLLLY QAMNSTFQLC KICAERDKDV 370 380 390 400 410 420 RIEPCGHLLC SCCLAAWQDS DSQTCPFCRC EIKGREAVSI CQAQERPTEV RTAADGSRDN 430 440 450 460 470 480 CHQEAAEQKL GPVIPSAPSL LPEDQFPQGP QDKGWLTLAP LALPRLRPPL PLPKMASVLW 490 EVTSRPRARE EATESS
	Isoform 2 [UniParc]. Checksum: 5EA7613675F647DF	FASTA	259	29,316
	10 20 30 40 50 60 MAAAAAPRGW QRGEPRALSR AVKLLQRLEE QCRDPRMVTG PPSLRDLLPR TAQLLGEVAK 70 80 90 100 110 120 ARREAREDPE GPGGADDFLA IYLANLEVKG RQVAELLPPR GKKDVNQDVF REGSRFRRQL 130 140 150 160 170 180 AKLALIFSHM HAELSALFPA GKYCGHLYQL TKGSAHIFWR QNCGVRCVLP WAEFQSLLCA 190 200 210 220 230 240 CHPVEPGPTM QALRSTLDLT CNGHVSVFEF DVFTRLFQPW PTLLRNWQLL AVNHPGYMAF 250 LTYDEVQTRL QAYRDKPGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the mouse Cblc/Cbl3 gene." Fiore F., Ollendorff V., Birnbaum D. Biochem. Biophys. Res. Commun. 280:182-187(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Tongue.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF319956 mRNA. Translation: AAK01405.1. AK009399 mRNA. Translation: BAB26265.1. AK010228 mRNA. Translation: BAB26782.1. BC046337 mRNA. Translation: AAH46337.1.
IPI	IPI00330742. IPI00649974.
RefSeq	NP_075713.3. NM_023224.5.
UniGene	Mm.81698.
3D structure databases
ProteinModelPortal	Q80XL1.
SMR	Q80XL1. Positions 8-400.
ModBase	Search...
PTM databases
PhosphoSite	Q80XL1.
Proteomic databases
PaxDb	Q80XL1.
PRIDE	Q80XL1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000043822; ENSMUSP00000039955; ENSMUSG00000040525.
GeneID	80794.
KEGG	mmu:80794.
UCSC	uc009fnh.2. mouse. uc009fni.2. mouse.
Organism-specific databases
CTD	23624.
MGI	MGI:1931457. Cblc.
Phylogenomic databases
eggNOG	NOG318595.
GeneTree	ENSGT00390000011617.
HOGENOM	HOG000294176.
HOVERGEN	HBG005255.
InParanoid	Q80XL1.
KO	K04707.
OMA	HSDSQTC.
OrthoDB	EOG4P8FJ1.
Gene expression databases
ArrayExpress	Q80XL1.
Bgee	Q80XL1.
CleanEx	MM_CBLC.
Genevestigator	Q80XL1.
GermOnline	ENSMUSG00000040525. Mus musculus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit. 1.20.930.20. 1 hit. 3.30.40.10. 1 hit. 3.30.505.10. 1 hit.
InterPro	IPR024162. Adaptor_Cbl. IPR014741. Adaptor_Cbl_EF_hand-like. IPR003153. Adaptor_Cbl_N_hlx. IPR014742. Adaptor_Cbl_SH2-like. IPR024159. Cbl_PTB. IPR011992. EF-hand-like_dom. IPR000980. SH2. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
PANTHER	PTHR23007. PTHR23007. 1 hit.
Pfam	PF02262. Cbl_N. 1 hit. PF02761. Cbl_N2. 1 hit. PF02762. Cbl_N3. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00252. SH2. 1 hit. [Graphical view]
SUPFAM	SSF47668. Adaptor_Cbl_N. 1 hit.
PROSITE	PS51506. CBL_PTB. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	350129.
SOURCE	Search...

Entry information

Entry name

CBLC_MOUSE

Accession

Primary (citable) accession number: Q80XL1
Secondary accession number(s): Q99PB6, Q9D6L2, Q9D7A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	May 29, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program