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Q80XI6 (M3K11_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase kinase kinase 11
EC=2.7.11.25
Alternative name(s):
Mixed lineage kinase 3
Gene names
Name:Map3k11
Synonyms:Mlk3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length850 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation By similarity.

Subunit structure

Homodimer; undergoes dimerization during activation By similarity. Interacts with MAP2K4/MKK4 and MAP2K7/MKK7 By similarity.

Subcellular location

Cytoplasm › cytoskeleton › centrosome. Note: Location is cell cycle dependent By similarity.

Post-translational modification

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-278 is likely to be the main autophosphorylation site. Phosphorylation of Ser-556 and Ser-557 is induced by CDC42 By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Contains 1 protein kinase domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   DomainRepeat
SH3 domain
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG1 phase of mitotic cell cycle

Inferred from electronic annotation. Source: Compara

activation of JUN kinase activity

Inferred from electronic annotation. Source: Compara

cell death

Inferred from electronic annotation. Source: Compara

cell proliferation

Inferred from electronic annotation. Source: Compara

microtubule-based process

Inferred from electronic annotation. Source: Compara

positive regulation of JNK cascade

Inferred from electronic annotation. Source: Compara

positive regulation of neuron apoptotic process

Inferred from electronic annotation. Source: Compara

protein autophosphorylation

Inferred from electronic annotation. Source: Compara

regulation of JNK cascade

Inferred from direct assay PubMed 12944902. Source: MGI

   Cellular_componentcentrosome

Inferred from electronic annotation. Source: Compara

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule

Inferred from electronic annotation. Source: Compara

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

JUN kinase kinase kinase activity

Inferred from electronic annotation. Source: Compara

protein tyrosine kinase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 850850Mitogen-activated protein kinase kinase kinase 11
PRO_0000277828

Regions

Domain42 – 10665SH3
Domain118 – 380263Protein kinase
Nucleotide binding124 – 1329ATP By similarity
Region404 – 42522Leucine-zipper 1
Region439 – 46022Leucine-zipper 2
Compositional bias18 – 2912Poly-Gly
Compositional bias473 – 4764Poly-Arg
Compositional bias600 – 823224Pro-rich

Sites

Active site2421Proton acceptor By similarity
Binding site1451ATP By similarity

Amino acid modifications

Modified residue111Phosphoserine By similarity
Modified residue2781Phosphothreonine; by autocatalysis By similarity
Modified residue2821Phosphoserine; by autocatalysis and MAP4K1 By similarity
Modified residue3951Phosphoserine By similarity
Modified residue5081Phosphoserine By similarity
Modified residue5251Phosphoserine By similarity
Modified residue5491Phosphoserine By similarity
Modified residue5561Phosphoserine By similarity
Modified residue5571Phosphoserine By similarity
Modified residue6551Phosphoserine By similarity
Modified residue7091Phosphoserine By similarity
Modified residue7121Phosphothreonine By similarity
Modified residue7281Phosphoserine By similarity
Modified residue7311Phosphoserine By similarity
Modified residue7431Phosphoserine Ref.3
Modified residue7511Phosphoserine By similarity
Modified residue7611Phosphoserine By similarity
Modified residue7731Phosphoserine By similarity
Modified residue7921Phosphoserine By similarity
Modified residue7961Phosphoserine By similarity
Modified residue8181Phosphoserine By similarity

Experimental info

Sequence conflict457 – 4582EL → DV in AAF73281. Ref.1
Sequence conflict840 – 8412AQ → CP in AAF73281. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q80XI6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 96DE238A1A0E2564

FASTA85093,226
        10         20         30         40         50         60 
MEPLKNLFLK SPLGSWNGSG SGGGGGTGGV RPEGSPKATA AYANPVWTAL FDYEPNGQDE 

        70         80         90        100        110        120 
LALRKGDRVE VLSRDAAISG DEGWWAGQVG GQVGIFPSNY VSRGGGPPPC EVASFQELRL 

       130        140        150        160        170        180 
EEVIGIGGFG KVYRGSWRGE LVAVKAARQD PDEDISVTAE SVRQEARLFA MLAHPNIIAL 

       190        200        210        220        230        240 
KAVCLEEPNL CLVMEYAAGG PLSRALAGRR VPPHVLVNWA VQIARGMHYL HCEALVPVIH 

       250        260        270        280        290        300 
RDLKSNNILL LQPIEGDDME HKTLKITDFG LAREWHKTTQ MSAAGTYAWM APEVIKASTF 

       310        320        330        340        350        360 
SKGSDVWSFG VLLWELLTGE VPYRGIDCLA VAYGVAVNKL TLPIPSTCPE PFAQLMADCW 

       370        380        390        400        410        420 
AQDPHRRPDF ASILQQLEAL EAQVLREMPR DSFHSMQEGW KREIQGLFDE LRAKEKELLS 

       430        440        450        460        470        480 
REEELTRAAR EQRSQAEQLR RREHLLAQWE LEVFERELTL LLQQVDRERP HVRRRRGTFK 

       490        500        510        520        530        540 
RSKLRARDGG ERISMPLDFK HRITVQASPG LDRRRNVFEV GAGDSPTFPR FRAIQLEPTE 

       550        560        570        580        590        600 
SGQTWGRQSP RRLEDSSNGE RRACWAWGPS SPKPGEAQNG RRRSRMDEAT WYLDSDDSSP 

       610        620        630        640        650        660 
LGSPSTPPAL NGNPPRPSPE PEEPRRAGPT ERGNSSGTPK LIQRALLRGT ALLASLGLGR 

       670        680        690        700        710        720 
DLQPPGGLSR ERGESPTAPP PAQMPSPCPP ELPSTPLIRL SQTTPDAHSS PTPGPLLLDL 

       730        740        750        760        770        780 
GVPSGQPSAK SPRREETRGR TVSPPPGISR SAPGTPGTPR SPPLGLISRP RPSPLRSRID 

       790        800        810        820        830        840 
PWSFVSAGPR PSPLPSPQPA PRRAPWTLFP DSDPFWDSPP ANPFRGGSQD CRTQTKDMGA 

       850 
QAPWAPEAGP

« Hide

References

« Hide 'large scale' references
[1]"Genomic sequencing reveals the structure of the Kcnk6 and map3k11 genes and their close vicinity to the sipa1 gene on mouse chromosome 19."
Saridaki A., Ferraz C., Demaille J., Scherer G., Roux A.-F.
Cytogenet. Cell Genet. 89:85-88(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Ola.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon, Eye and Salivary gland.
[3]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-743, MASS SPECTROMETRY.
Tissue: Macrophage.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF155142 Genomic DNA. Translation: AAF73281.1.
BC030928 mRNA. Translation: AAH30928.1.
BC047152 mRNA. Translation: AAH47152.1.
BC095963 mRNA. Translation: AAH95963.1.
IPIIPI00330731.
RefSeqNP_071295.2. NM_022012.3.
UniGeneMm.185026.

3D structure databases

HSSPHSSP built from PDB template 1U4D based on UniProtKB Q07912.
ProteinModelPortalQ80XI6.
SMRQ80XI6. Positions 45-102, 115-460.
ModBaseSearch...

Protein-protein interaction databases

IntActQ80XI6. 2 interactions.

PTM databases

PhosphoSiteQ80XI6.

Proteomic databases

PaxDbQ80XI6.
PRIDEQ80XI6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000004156; ENSMUSP00000004156; ENSMUSG00000004054.
GeneID26403.
KEGGmmu:26403.
UCSCuc008get.1. mouse.

Organism-specific databases

CTD4296.
MGIMGI:1346880. Map3k11.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00700000104091.
HOGENOMHOG000060081.
HOVERGENHBG067662.
InParanoidQ80XI6.
KOK04419.
OMASGTPKLI.
OrthoDBEOG4PNXGN.

Gene expression databases

BgeeQ80XI6.
CleanExMM_MAP3K11.
GenevestigatorQ80XI6.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR016231. MAPKKK9/10/11.
IPR015785. MAPKKK9/10/11-like.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PANTHERPTHR23257:SF87. PTHR23257:SF87. 1 hit.
PfamPF07714. Pkinase_Tyr. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PIRSFPIRSF000556. MAPKKK9_11. 1 hit.
PRINTSPR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTSM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
SSF50044. SH3. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMAP3K11. mouse.
NextBio304369.
SOURCESearch...

Entry information

Entry nameM3K11_MOUSE
AccessionPrimary (citable) accession number: Q80XI6
Secondary accession number(s): Q8K0M8, Q9JJ15
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents