Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q80XI6

- M3K11_MOUSE

UniProt

Q80XI6 - M3K11_MOUSE

Protein

Mitogen-activated protein kinase kinase kinase 11

Gene

Map3k11

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei145 – 1451ATPPROSITE-ProRule annotation
    Active sitei242 – 2421Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi124 – 1329ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. JUN kinase kinase kinase activity Source: Ensembl
    3. protein binding Source: MGI
    4. protein tyrosine kinase activity Source: InterPro

    GO - Biological processi

    1. activation of JUN kinase activity Source: Ensembl
    2. cell death Source: Ensembl
    3. cell proliferation Source: Ensembl
    4. microtubule-based process Source: Ensembl
    5. positive regulation of JNK cascade Source: Ensembl
    6. positive regulation of neuron apoptotic process Source: Ensembl
    7. protein autophosphorylation Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase kinase kinase 11 (EC:2.7.11.25)
    Alternative name(s):
    Mixed lineage kinase 3
    Gene namesi
    Name:Map3k11
    Synonyms:Mlk3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1346880. Map3k11.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome
    Note: Location is cell cycle dependent.By similarity

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. cytoplasm Source: UniProtKB-KW
    3. microtubule Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 850850Mitogen-activated protein kinase kinase kinase 11PRO_0000277828Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei11 – 111PhosphoserineBy similarity
    Modified residuei278 – 2781Phosphothreonine; by autocatalysisBy similarity
    Modified residuei282 – 2821Phosphoserine; by autocatalysis and MAP4K1By similarity
    Modified residuei395 – 3951PhosphoserineBy similarity
    Modified residuei508 – 5081PhosphoserineBy similarity
    Modified residuei525 – 5251PhosphoserineBy similarity
    Modified residuei549 – 5491PhosphoserineBy similarity
    Modified residuei556 – 5561PhosphoserineBy similarity
    Modified residuei557 – 5571PhosphoserineBy similarity
    Modified residuei655 – 6551PhosphoserineBy similarity
    Modified residuei709 – 7091PhosphoserineBy similarity
    Modified residuei712 – 7121PhosphothreonineBy similarity
    Modified residuei728 – 7281PhosphoserineBy similarity
    Modified residuei731 – 7311PhosphoserineBy similarity
    Modified residuei743 – 7431Phosphoserine1 Publication
    Modified residuei751 – 7511PhosphoserineBy similarity
    Modified residuei761 – 7611PhosphoserineBy similarity
    Modified residuei773 – 7731PhosphoserineBy similarity
    Modified residuei792 – 7921PhosphoserineBy similarity
    Modified residuei796 – 7961PhosphoserineBy similarity
    Modified residuei818 – 8181PhosphoserineBy similarity

    Post-translational modificationi

    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-278 is likely to be the main autophosphorylation site. Phosphorylation of Ser-556 and Ser-557 is induced by CDC42 By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ80XI6.
    PRIDEiQ80XI6.

    PTM databases

    PhosphoSiteiQ80XI6.

    Expressioni

    Gene expression databases

    BgeeiQ80XI6.
    CleanExiMM_MAP3K11.
    GenevestigatoriQ80XI6.

    Interactioni

    Subunit structurei

    Homodimer; undergoes dimerization during activation. Interacts with MAP2K4/MKK4 and MAP2K7/MKK7.By similarity

    Protein-protein interaction databases

    BioGridi204956. 2 interactions.
    IntActiQ80XI6. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80XI6.
    SMRiQ80XI6. Positions 45-102, 115-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 10665SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini118 – 380263Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni404 – 42522Leucine-zipper 1Add
    BLAST
    Regioni439 – 46022Leucine-zipper 2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi18 – 2912Poly-GlyAdd
    BLAST
    Compositional biasi473 – 4764Poly-Arg
    Compositional biasi600 – 823224Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117613.
    HOGENOMiHOG000060081.
    HOVERGENiHBG067662.
    InParanoidiQ80XI6.
    KOiK04419.
    OMAiAWGRQSP.
    OrthoDBiEOG7D85VN.
    PhylomeDBiQ80XI6.
    TreeFamiTF105118.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR016231. MAPKKK9/10/11.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR001452. SH3_domain.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000556. MAPKKK9_11. 1 hit.
    PRINTSiPR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80XI6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPLKNLFLK SPLGSWNGSG SGGGGGTGGV RPEGSPKATA AYANPVWTAL    50
    FDYEPNGQDE LALRKGDRVE VLSRDAAISG DEGWWAGQVG GQVGIFPSNY 100
    VSRGGGPPPC EVASFQELRL EEVIGIGGFG KVYRGSWRGE LVAVKAARQD 150
    PDEDISVTAE SVRQEARLFA MLAHPNIIAL KAVCLEEPNL CLVMEYAAGG 200
    PLSRALAGRR VPPHVLVNWA VQIARGMHYL HCEALVPVIH RDLKSNNILL 250
    LQPIEGDDME HKTLKITDFG LAREWHKTTQ MSAAGTYAWM APEVIKASTF 300
    SKGSDVWSFG VLLWELLTGE VPYRGIDCLA VAYGVAVNKL TLPIPSTCPE 350
    PFAQLMADCW AQDPHRRPDF ASILQQLEAL EAQVLREMPR DSFHSMQEGW 400
    KREIQGLFDE LRAKEKELLS REEELTRAAR EQRSQAEQLR RREHLLAQWE 450
    LEVFERELTL LLQQVDRERP HVRRRRGTFK RSKLRARDGG ERISMPLDFK 500
    HRITVQASPG LDRRRNVFEV GAGDSPTFPR FRAIQLEPTE SGQTWGRQSP 550
    RRLEDSSNGE RRACWAWGPS SPKPGEAQNG RRRSRMDEAT WYLDSDDSSP 600
    LGSPSTPPAL NGNPPRPSPE PEEPRRAGPT ERGNSSGTPK LIQRALLRGT 650
    ALLASLGLGR DLQPPGGLSR ERGESPTAPP PAQMPSPCPP ELPSTPLIRL 700
    SQTTPDAHSS PTPGPLLLDL GVPSGQPSAK SPRREETRGR TVSPPPGISR 750
    SAPGTPGTPR SPPLGLISRP RPSPLRSRID PWSFVSAGPR PSPLPSPQPA 800
    PRRAPWTLFP DSDPFWDSPP ANPFRGGSQD CRTQTKDMGA QAPWAPEAGP 850
    Length:850
    Mass (Da):93,226
    Last modified:June 1, 2003 - v1
    Checksum:i96DE238A1A0E2564
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti457 – 4582EL → DV in AAF73281. (PubMed:10894943)Curated
    Sequence conflicti840 – 8412AQ → CP in AAF73281. (PubMed:10894943)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF155142 Genomic DNA. Translation: AAF73281.1.
    BC030928 mRNA. Translation: AAH30928.1.
    BC047152 mRNA. Translation: AAH47152.1.
    BC095963 mRNA. Translation: AAH95963.1.
    CCDSiCCDS29476.1.
    RefSeqiNP_071295.2. NM_022012.3.
    XP_006531815.1. XM_006531752.1.
    UniGeneiMm.185026.

    Genome annotation databases

    EnsembliENSMUST00000004156; ENSMUSP00000004156; ENSMUSG00000004054.
    GeneIDi26403.
    KEGGimmu:26403.
    UCSCiuc008get.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF155142 Genomic DNA. Translation: AAF73281.1 .
    BC030928 mRNA. Translation: AAH30928.1 .
    BC047152 mRNA. Translation: AAH47152.1 .
    BC095963 mRNA. Translation: AAH95963.1 .
    CCDSi CCDS29476.1.
    RefSeqi NP_071295.2. NM_022012.3.
    XP_006531815.1. XM_006531752.1.
    UniGenei Mm.185026.

    3D structure databases

    ProteinModelPortali Q80XI6.
    SMRi Q80XI6. Positions 45-102, 115-460.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204956. 2 interactions.
    IntActi Q80XI6. 2 interactions.

    Chemistry

    ChEMBLi CHEMBL2434817.

    PTM databases

    PhosphoSitei Q80XI6.

    Proteomic databases

    PaxDbi Q80XI6.
    PRIDEi Q80XI6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000004156 ; ENSMUSP00000004156 ; ENSMUSG00000004054 .
    GeneIDi 26403.
    KEGGi mmu:26403.
    UCSCi uc008get.1. mouse.

    Organism-specific databases

    CTDi 4296.
    MGIi MGI:1346880. Map3k11.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117613.
    HOGENOMi HOG000060081.
    HOVERGENi HBG067662.
    InParanoidi Q80XI6.
    KOi K04419.
    OMAi AWGRQSP.
    OrthoDBi EOG7D85VN.
    PhylomeDBi Q80XI6.
    TreeFami TF105118.

    Miscellaneous databases

    ChiTaRSi MAP3K11. mouse.
    NextBioi 304369.
    PROi Q80XI6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q80XI6.
    CleanExi MM_MAP3K11.
    Genevestigatori Q80XI6.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR016231. MAPKKK9/10/11.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR001452. SH3_domain.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000556. MAPKKK9_11. 1 hit.
    PRINTSi PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequencing reveals the structure of the Kcnk6 and map3k11 genes and their close vicinity to the sipa1 gene on mouse chromosome 19."
      Saridaki A., Ferraz C., Demaille J., Scherer G., Roux A.-F.
      Cytogenet. Cell Genet. 89:85-88(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Ola.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon, Eye and Salivary gland.
    3. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-743, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiM3K11_MOUSE
    AccessioniPrimary (citable) accession number: Q80XI6
    Secondary accession number(s): Q8K0M8, Q9JJ15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3