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Protein

Eukaryotic translation initiation factor 4 gamma 3

Gene

Eif4g3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • positive regulation of meiosis I Source: MGI
  • positive regulation of protein phosphorylation Source: MGI
  • positive regulation of translation Source: MGI
  • spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4 gamma 3
Short name:
eIF-4-gamma 3
Short name:
eIF-4G 3
Short name:
eIF4G 3
Alternative name(s):
eIF-4-gamma II
Short name:
eIF4GII
Gene namesi
Name:Eif4g3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1923935. Eif4g3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002133301 – 1579Eukaryotic translation initiation factor 4 gamma 3Add BLAST1579

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei168PhosphothreonineCombined sources1
Modified residuei230PhosphoserineBy similarity1
Modified residuei232PhosphoserineBy similarity1
Modified residuei267PhosphoserineCombined sources1
Modified residuei436PhosphoserineCombined sources1
Modified residuei470PhosphoserineCombined sources1
Modified residuei472PhosphoserineCombined sources1
Modified residuei490PhosphoserineBy similarity1
Modified residuei1150Phosphoserine; by CaMK1By similarity1
Modified residuei1212PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80XI3.
PaxDbiQ80XI3.
PeptideAtlasiQ80XI3.
PRIDEiQ80XI3.

PTM databases

iPTMnetiQ80XI3.
PhosphoSitePlusiQ80XI3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028760.

Interactioni

Subunit structurei

Interacts with EIF4A, EIF4E, eIF3 and PABPC1. Part of a complex with EIF4E. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4G1/EIF4G3 interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate eIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIFG3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA (By similarity).By similarity

Protein-protein interaction databases

BioGridi231042. 2 interactors.
IntActiQ80XI3. 1 interactor.
MINTiMINT-1864762.
STRINGi10090.ENSMUSP00000081232.

Structurei

3D structure databases

ProteinModelPortaliQ80XI3.
SMRiQ80XI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati740 – 778HEAT 1Add BLAST39
Domaini750 – 978MIF4GPROSITE-ProRule annotationAdd BLAST229
Repeati779 – 826HEAT 2Add BLAST48
Repeati827 – 900HEAT 3Add BLAST74
Repeati901 – 939HEAT 4Add BLAST39
Repeati940 – 979HEAT 5Add BLAST40
Domaini1215 – 1337MIPROSITE-ProRule annotationAdd BLAST123
Domaini1410 – 1579W2PROSITE-ProRule annotationAdd BLAST170

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 162PABPC1-bindingBy similarityAdd BLAST29
Regioni614 – 625EIF4E-bindingBy similarityAdd BLAST12
Regioni694 – 1014eIF3/EIF4A-bindingBy similarityAdd BLAST321
Regioni1427 – 1579EIF4A-bindingBy similarityAdd BLAST153
Regioni1565 – 1579Necessary but not sufficient for MKNK1-bindingBy similarityAdd BLAST15

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili989 – 1018Sequence analysisAdd BLAST30
Coiled coili1154 – 1176Sequence analysisAdd BLAST23
Coiled coili1406 – 1438Sequence analysisAdd BLAST33

Sequence similaritiesi

Contains 5 HEAT repeats.Curated
Contains 1 MI domain.PROSITE-ProRule annotation
Contains 1 MIF4G domain.Curated
Contains 1 W2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0401. Eukaryota.
ENOG410XS4P. LUCA.
HOVERGENiHBG052083.
InParanoidiQ80XI3.
KOiK03260.
PhylomeDBiQ80XI3.

Family and domain databases

Gene3Di1.25.40.180. 3 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
IPR003307. W2_domain.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
PF02854. MIF4G. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
SM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS51366. MI. 1 hit.
PS51363. W2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80XI3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSQPQARSP FFQRPQIQPP RAAIPNSSPS IRPGVQTPTA VYQANQHIMM
60 70 80 90 100
VNHLPMPYPV TQGHQYCIPQ YRHSGPPYVG PPQQYPVQPP GPGPFYPGPG
110 120 130 140 150
PGDFANAYGT PFYPSQPVYQ SAPIIVPTQQ QPPPAKREKK TIRIRDPNQG
160 170 180 190 200
GKDITEEIMS GGGSRNPTPP IGRPASTPTP PQQLPSQVPE HSPVVYGTVE
210 220 230 240 250
SAHLAASTPV TAASDQKQEE KPKPDPVFQS PSTVLRLVLS GEKKEQAGQM
260 270 280 290 300
PETAAGEPTP EPPRTSSPTS LPPLARSSLP SPMSAALSSQ PLFTAEDKCE
310 320 330 340 350
LPSSKEEDAP PVPSPTSCTA ASGPSLTDNS DICKKPCSVA PHDSQLISST
360 370 380 390 400
ILINEMNGVG EKLSAKENTV GMLRQEVLPL TLELEILEHP QEELKVECTP
410 420 430 440 450
TPIAPSMLPA FSPAPPTPPT SPPCPPVVLS AAIARSPAVA TEVQRVADEG
460 470 480 490 500
ESLRTCLSKD AKEMQDKAES ESDGQAEETA DPQSLHSGRS PAPVQTATTA
510 520 530 540 550
PKSWKKTKEQ TRTPDEVLEA EAEPKAEEEL AVDSVLEPEQ EKMSQGFPSE
560 570 580 590 600
RDPSALKRGK AEEGNGEEAE PVRNGAESAS EGEGGDGNSG SADSSADGLT
610 620 630 640 650
FPFKAESWKP ADTEGKKQYD REFLLDIQFM PACIQKPEGL PPISDVVLDK
660 670 680 690 700
INQPRLSMRT LDPRILPRGP DFTPAFADFP RQTPGGRGVP LLNVGPRRSQ
710 720 730 740 750
PGQRREPRKI ITVSVKEDVH LRKAENAWKP SQKRDSHADD PESIKTQELF
760 770 780 790 800
RKVRSILNKL TPQMFNQLMK QVSALTVDTE ERLKGVIDLV FEKAIDEPSF
810 820 830 840 850
SVAYANMCRC LVTLKVPMAD KPGNTVNFRK LLLNRCQKEF EKDKADDDVF
860 870 880 890 900
EKKQKELEAA SAPEERTRLH DELEEAKDKA RRRSIGNIKF IGELFKLKML
910 920 930 940 950
TEAIMHDCVV KLLKNHDEES LECLCRLLTT IGKDLDFEKA KPRMDQYFNQ
960 970 980 990 1000
MEKIVKERKT SSRIRFMLQD VIDLRLCNWV SRRADQGPKT IEQIHKEAKI
1010 1020 1030 1040 1050
EEQEEQRKVQ QLMTKEKRRP GVQRVDEGGW NTVQGAKNSR VLDPSKFLKI
1060 1070 1080 1090 1100
TKPTIDEKIQ LVPKAQLGSW GKGSSGGAKA SESDALRSSA SSLNRFSPLQ
1110 1120 1130 1140 1150
PPAPSGSPSA TPLEFDSRRA LTSRGSMGRE KSDKPIPAGT ARPNTFLRGS
1160 1170 1180 1190 1200
SKDLLDNQSQ EEQRREMLET VKQLTGGLDA ERASTEADRS KTRELAKSEM
1210 1220 1230 1240 1250
CAVPAPDKPA LSEEEVERKS KSIIDEFLHI NDFKEATQCI EELSAQGPLH
1260 1270 1280 1290 1300
VFVKVGVEFT LERSQITRDH MGHLLYQLVQ SEKLSKQDFF KGFSETLELA
1310 1320 1330 1340 1350
DDMAIDIPHI WLYLAELVTP MLKEGGISMR ELIVEFSKPL LPVGRAGVLL
1360 1370 1380 1390 1400
SEILHLLCRQ MSHKKVGALW READLSWKDF LPEGEDVHHF LLEQKLDFTE
1410 1420 1430 1440 1450
SEGPCSSEAL SKKELSAEEL SQRLEKLIME EKADDERIFD WVEANLDESQ
1460 1470 1480 1490 1500
MSSPTFLRAL MTAVCKAAII ADCSTFRVDT AVIKQRVPIL LKYLDSDTEK
1510 1520 1530 1540 1550
ELQALYALQA SIVKLDQPAN LLRMFFDCLY DEEVISEDAF YKWESSKDPA
1560 1570
EQAGKGVALK SVTAFFTWLR EAEEESEDN
Length:1,579
Mass (Da):174,890
Last modified:June 7, 2004 - v2
Checksum:iEB4854590D250450
GO
Isoform 2 (identifier: Q80XI3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1084-1102: Missing.

Show »
Length:1,560
Mass (Da):172,865
Checksum:iEBE892F0BBC70182
GO
Isoform 3 (identifier: Q80XI3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     11-11: F → FAAGPRPAHHQF

Note: No experimental confirmation available.
Show »
Length:1,590
Mass (Da):176,060
Checksum:i384A47C5E2EE294C
GO
Isoform 4 (identifier: Q80XI3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: P → PFAAGPRPAHHQGGFRPIQ
     1084-1102: Missing.

Show »
Length:1,578
Mass (Da):174,791
Checksum:i05FEAAD3A5AB616D
GO

Sequence cautioni

The sequence AAH23898 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
The sequence AAH23898 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti183Missing in CF743072 (Ref. 2) Curated1
Sequence conflicti410A → G in CF743072 (Ref. 2) Curated1
Sequence conflicti492A → P in CB522417 (Ref. 2) Curated1
Sequence conflicti1324E → G in BAC26452 (PubMed:16141072).Curated1
Sequence conflicti1324E → G in AAH72600 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02602910P → PFAAGPRPAHHQGGFRPIQ in isoform 4. 1 Publication1
Alternative sequenceiVSP_01049011F → FAAGPRPAHHQF in isoform 3. Curated1
Alternative sequenceiVSP_0104911084 – 1102Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029440 mRNA. Translation: BAC26452.1.
AK050887 mRNA. Translation: BAC34445.1.
AK054068 mRNA. Translation: BAC35644.1.
CB522417 mRNA. No translation available.
CF743072 mRNA. No translation available.
BC023898 mRNA. Translation: AAH23898.1. Different initiation.
BC047531 mRNA. Translation: AAH47531.1.
BC048848 mRNA. Translation: AAH48848.1.
BC057913 mRNA. Translation: AAH57913.1.
BC072600 mRNA. Translation: AAH72600.1.
CCDSiCCDS38926.1. [Q80XI3-4]
RefSeqiNP_766291.2. NM_172703.3.
UniGeneiMm.268903.
Mm.407375.

Genome annotation databases

GeneIDi230861.
KEGGimmu:230861.
UCSCiuc008vka.2. mouse. [Q80XI3-4]
uc008vkb.2. mouse. [Q80XI3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029440 mRNA. Translation: BAC26452.1.
AK050887 mRNA. Translation: BAC34445.1.
AK054068 mRNA. Translation: BAC35644.1.
CB522417 mRNA. No translation available.
CF743072 mRNA. No translation available.
BC023898 mRNA. Translation: AAH23898.1. Different initiation.
BC047531 mRNA. Translation: AAH47531.1.
BC048848 mRNA. Translation: AAH48848.1.
BC057913 mRNA. Translation: AAH57913.1.
BC072600 mRNA. Translation: AAH72600.1.
CCDSiCCDS38926.1. [Q80XI3-4]
RefSeqiNP_766291.2. NM_172703.3.
UniGeneiMm.268903.
Mm.407375.

3D structure databases

ProteinModelPortaliQ80XI3.
SMRiQ80XI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231042. 2 interactors.
IntActiQ80XI3. 1 interactor.
MINTiMINT-1864762.
STRINGi10090.ENSMUSP00000081232.

PTM databases

iPTMnetiQ80XI3.
PhosphoSitePlusiQ80XI3.

Proteomic databases

EPDiQ80XI3.
PaxDbiQ80XI3.
PeptideAtlasiQ80XI3.
PRIDEiQ80XI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi230861.
KEGGimmu:230861.
UCSCiuc008vka.2. mouse. [Q80XI3-4]
uc008vkb.2. mouse. [Q80XI3-2]

Organism-specific databases

CTDi8672.
MGIiMGI:1923935. Eif4g3.

Phylogenomic databases

eggNOGiKOG0401. Eukaryota.
ENOG410XS4P. LUCA.
HOVERGENiHBG052083.
InParanoidiQ80XI3.
KOiK03260.
PhylomeDBiQ80XI3.

Miscellaneous databases

ChiTaRSiEif4g3. mouse.
PROiQ80XI3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028760.

Family and domain databases

Gene3Di1.25.40.180. 3 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
IPR003307. W2_domain.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
PF02854. MIF4G. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
SM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS51366. MI. 1 hit.
PS51363. W2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4G3_MOUSE
AccessioniPrimary (citable) accession number: Q80XI3
Secondary accession number(s): Q6GQV5
, Q80Y69, Q8BJ68, Q8BQF3, Q8C6Q3, Q8CIH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.