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Q80XI3

- IF4G3_MOUSE

UniProt

Q80XI3 - IF4G3_MOUSE

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Protein

Eukaryotic translation initiation factor 4 gamma 3

Gene
Eif4g3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1 By similarity.

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. translation initiation factor activity Source: UniProtKB-KW

GO - Biological processi

  1. positive regulation of meiosis I Source: MGI
  2. positive regulation of protein phosphorylation Source: MGI
  3. positive regulation of translation Source: MGI
  4. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiREACT_198533. ISG15 antiviral mechanism.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4 gamma 3
Short name:
eIF-4-gamma 3
Short name:
eIF-4G 3
Short name:
eIF4G 3
Alternative name(s):
eIF-4-gamma II
Short name:
eIF4GII
Gene namesi
Name:Eif4g3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1923935. Eif4g3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15791579Eukaryotic translation initiation factor 4 gamma 3PRO_0000213330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei168 – 1681Phosphothreonine1 Publication
Modified residuei230 – 2301Phosphoserine By similarity
Modified residuei232 – 2321Phosphoserine By similarity
Modified residuei267 – 2671Phosphoserine1 Publication
Modified residuei470 – 4701Phosphoserine1 Publication
Modified residuei472 – 4721Phosphoserine2 Publications
Modified residuei490 – 4901Phosphoserine By similarity
Modified residuei1150 – 11501Phosphoserine; by CaMK1 By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80XI3.
PRIDEiQ80XI3.

PTM databases

PhosphoSiteiQ80XI3.

Expressioni

Gene expression databases

ArrayExpressiQ80XI3.
BgeeiQ80XI3.
GenevestigatoriQ80XI3.

Interactioni

Subunit structurei

Interacts with EIF4A, EIF4E, eIF3 and PABPC1. Part of a complex with EIF4E. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4G1/EIF4G3 interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate eIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIFG3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA By similarity.

Protein-protein interaction databases

BioGridi231042. 2 interactions.
MINTiMINT-1864762.

Structurei

3D structure databases

ProteinModelPortaliQ80XI3.
SMRiQ80XI3. Positions 136-161, 740-981, 1208-1545.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati740 – 77839HEAT 1Add
BLAST
Domaini750 – 978229MIF4GAdd
BLAST
Repeati779 – 82648HEAT 2Add
BLAST
Repeati827 – 90074HEAT 3Add
BLAST
Repeati901 – 93939HEAT 4Add
BLAST
Repeati940 – 97940HEAT 5Add
BLAST
Domaini1215 – 1337123MIAdd
BLAST
Domaini1410 – 1579170W2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 16229PABPC1-binding By similarityAdd
BLAST
Regioni614 – 62512EIF4E-binding By similarityAdd
BLAST
Regioni694 – 1014321eIF3/EIF4A-binding By similarityAdd
BLAST
Regioni1427 – 1579153EIF4A-binding By similarityAdd
BLAST
Regioni1565 – 157915Necessary but not sufficient for MKNK1-binding By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili989 – 101830 Reviewed predictionAdd
BLAST
Coiled coili1154 – 117623 Reviewed predictionAdd
BLAST
Coiled coili1406 – 143833 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Contains 5 HEAT repeats.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Contains 1 W2 domain.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG301289.
GeneTreeiENSGT00530000063038.
HOVERGENiHBG052083.
KOiK03260.
PhylomeDBiQ80XI3.

Family and domain databases

Gene3Di1.25.40.180. 3 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like_typ_1/2/3.
IPR003890. MIF4G-like_typ-3.
IPR003307. W2_domain.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
PF02854. MIF4G. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
SM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS51366. MI. 1 hit.
PS51363. W2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q80XI3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MNSQPQARSP FFQRPQIQPP RAAIPNSSPS IRPGVQTPTA VYQANQHIMM     50
VNHLPMPYPV TQGHQYCIPQ YRHSGPPYVG PPQQYPVQPP GPGPFYPGPG 100
PGDFANAYGT PFYPSQPVYQ SAPIIVPTQQ QPPPAKREKK TIRIRDPNQG 150
GKDITEEIMS GGGSRNPTPP IGRPASTPTP PQQLPSQVPE HSPVVYGTVE 200
SAHLAASTPV TAASDQKQEE KPKPDPVFQS PSTVLRLVLS GEKKEQAGQM 250
PETAAGEPTP EPPRTSSPTS LPPLARSSLP SPMSAALSSQ PLFTAEDKCE 300
LPSSKEEDAP PVPSPTSCTA ASGPSLTDNS DICKKPCSVA PHDSQLISST 350
ILINEMNGVG EKLSAKENTV GMLRQEVLPL TLELEILEHP QEELKVECTP 400
TPIAPSMLPA FSPAPPTPPT SPPCPPVVLS AAIARSPAVA TEVQRVADEG 450
ESLRTCLSKD AKEMQDKAES ESDGQAEETA DPQSLHSGRS PAPVQTATTA 500
PKSWKKTKEQ TRTPDEVLEA EAEPKAEEEL AVDSVLEPEQ EKMSQGFPSE 550
RDPSALKRGK AEEGNGEEAE PVRNGAESAS EGEGGDGNSG SADSSADGLT 600
FPFKAESWKP ADTEGKKQYD REFLLDIQFM PACIQKPEGL PPISDVVLDK 650
INQPRLSMRT LDPRILPRGP DFTPAFADFP RQTPGGRGVP LLNVGPRRSQ 700
PGQRREPRKI ITVSVKEDVH LRKAENAWKP SQKRDSHADD PESIKTQELF 750
RKVRSILNKL TPQMFNQLMK QVSALTVDTE ERLKGVIDLV FEKAIDEPSF 800
SVAYANMCRC LVTLKVPMAD KPGNTVNFRK LLLNRCQKEF EKDKADDDVF 850
EKKQKELEAA SAPEERTRLH DELEEAKDKA RRRSIGNIKF IGELFKLKML 900
TEAIMHDCVV KLLKNHDEES LECLCRLLTT IGKDLDFEKA KPRMDQYFNQ 950
MEKIVKERKT SSRIRFMLQD VIDLRLCNWV SRRADQGPKT IEQIHKEAKI 1000
EEQEEQRKVQ QLMTKEKRRP GVQRVDEGGW NTVQGAKNSR VLDPSKFLKI 1050
TKPTIDEKIQ LVPKAQLGSW GKGSSGGAKA SESDALRSSA SSLNRFSPLQ 1100
PPAPSGSPSA TPLEFDSRRA LTSRGSMGRE KSDKPIPAGT ARPNTFLRGS 1150
SKDLLDNQSQ EEQRREMLET VKQLTGGLDA ERASTEADRS KTRELAKSEM 1200
CAVPAPDKPA LSEEEVERKS KSIIDEFLHI NDFKEATQCI EELSAQGPLH 1250
VFVKVGVEFT LERSQITRDH MGHLLYQLVQ SEKLSKQDFF KGFSETLELA 1300
DDMAIDIPHI WLYLAELVTP MLKEGGISMR ELIVEFSKPL LPVGRAGVLL 1350
SEILHLLCRQ MSHKKVGALW READLSWKDF LPEGEDVHHF LLEQKLDFTE 1400
SEGPCSSEAL SKKELSAEEL SQRLEKLIME EKADDERIFD WVEANLDESQ 1450
MSSPTFLRAL MTAVCKAAII ADCSTFRVDT AVIKQRVPIL LKYLDSDTEK 1500
ELQALYALQA SIVKLDQPAN LLRMFFDCLY DEEVISEDAF YKWESSKDPA 1550
EQAGKGVALK SVTAFFTWLR EAEEESEDN 1579
Length:1,579
Mass (Da):174,890
Last modified:June 7, 2004 - v2
Checksum:iEB4854590D250450
GO
Isoform 2 (identifier: Q80XI3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1084-1102: Missing.

Show »
Length:1,560
Mass (Da):172,865
Checksum:iEBE892F0BBC70182
GO
Isoform 3 (identifier: Q80XI3-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     11-11: F → FAAGPRPAHHQF

Note: No experimental confirmation available.

Show »
Length:1,590
Mass (Da):176,060
Checksum:i384A47C5E2EE294C
GO
Isoform 4 (identifier: Q80XI3-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: P → PFAAGPRPAHHQGGFRPIQ
     1084-1102: Missing.

Show »
Length:1,578
Mass (Da):174,791
Checksum:i05FEAAD3A5AB616D
GO

Sequence cautioni

The sequence AAH23898.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part.
The sequence AAH23898.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei10 – 101P → PFAAGPRPAHHQGGFRPIQ in isoform 4. VSP_026029
Alternative sequencei11 – 111F → FAAGPRPAHHQF in isoform 3. VSP_010490
Alternative sequencei1084 – 110219Missing in isoform 2 and isoform 4. VSP_010491Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831Missing in CF743072. 1 Publication
Sequence conflicti410 – 4101A → G in CF743072. 1 Publication
Sequence conflicti492 – 4921A → P in CB522417. 1 Publication
Sequence conflicti1324 – 13241E → G in BAC26452. 1 Publication
Sequence conflicti1324 – 13241E → G in AAH72600. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK029440 mRNA. Translation: BAC26452.1.
AK050887 mRNA. Translation: BAC34445.1.
AK054068 mRNA. Translation: BAC35644.1.
CB522417 mRNA. No translation available.
CF743072 mRNA. No translation available.
BC023898 mRNA. Translation: AAH23898.1. Different initiation.
BC047531 mRNA. Translation: AAH47531.1.
BC048848 mRNA. Translation: AAH48848.1.
BC057913 mRNA. Translation: AAH57913.1.
BC072600 mRNA. Translation: AAH72600.1.
CCDSiCCDS38926.1. [Q80XI3-4]
RefSeqiNP_766291.2. NM_172703.3.
UniGeneiMm.268903.
Mm.407375.

Genome annotation databases

EnsembliENSMUST00000084214; ENSMUSP00000081232; ENSMUSG00000028760.
GeneIDi230861.
KEGGimmu:230861.
UCSCiuc008vka.2. mouse. [Q80XI3-4]
uc008vkb.2. mouse. [Q80XI3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK029440 mRNA. Translation: BAC26452.1 .
AK050887 mRNA. Translation: BAC34445.1 .
AK054068 mRNA. Translation: BAC35644.1 .
CB522417 mRNA. No translation available.
CF743072 mRNA. No translation available.
BC023898 mRNA. Translation: AAH23898.1 . Different initiation.
BC047531 mRNA. Translation: AAH47531.1 .
BC048848 mRNA. Translation: AAH48848.1 .
BC057913 mRNA. Translation: AAH57913.1 .
BC072600 mRNA. Translation: AAH72600.1 .
CCDSi CCDS38926.1. [Q80XI3-4 ]
RefSeqi NP_766291.2. NM_172703.3.
UniGenei Mm.268903.
Mm.407375.

3D structure databases

ProteinModelPortali Q80XI3.
SMRi Q80XI3. Positions 136-161, 740-981, 1208-1545.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 231042. 2 interactions.
MINTi MINT-1864762.

PTM databases

PhosphoSitei Q80XI3.

Proteomic databases

PaxDbi Q80XI3.
PRIDEi Q80XI3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000084214 ; ENSMUSP00000081232 ; ENSMUSG00000028760 .
GeneIDi 230861.
KEGGi mmu:230861.
UCSCi uc008vka.2. mouse. [Q80XI3-4 ]
uc008vkb.2. mouse. [Q80XI3-2 ]

Organism-specific databases

CTDi 8672.
MGIi MGI:1923935. Eif4g3.

Phylogenomic databases

eggNOGi NOG301289.
GeneTreei ENSGT00530000063038.
HOVERGENi HBG052083.
KOi K03260.
PhylomeDBi Q80XI3.

Enzyme and pathway databases

Reactomei REACT_198533. ISG15 antiviral mechanism.

Miscellaneous databases

ChiTaRSi EIF4G3. mouse.
NextBioi 380218.
PROi Q80XI3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q80XI3.
Bgeei Q80XI3.
Genevestigatori Q80XI3.

Family and domain databases

Gene3Di 1.25.40.180. 3 hits.
InterProi IPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like_typ_1/2/3.
IPR003890. MIF4G-like_typ-3.
IPR003307. W2_domain.
[Graphical view ]
Pfami PF02847. MA3. 1 hit.
PF02854. MIF4G. 1 hit.
PF02020. W2. 1 hit.
[Graphical view ]
SMARTi SM00515. eIF5C. 1 hit.
SM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 3 hits.
PROSITEi PS51366. MI. 1 hit.
PS51363. W2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-217 AND 807-1579 (ISOFORM 2), NUCLEOTIDE SEQUENCE OF 1-135 (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Adipose tissue, Head and Oviduct.
  2. The MGC Project Team
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 164-492.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain, Colon, Kidney, Liver and Mammary tumor.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-168; SER-267; SER-470 AND SER-472, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIF4G3_MOUSE
AccessioniPrimary (citable) accession number: Q80XI3
Secondary accession number(s): Q6GQV5
, Q80Y69, Q8BJ68, Q8BQF3, Q8C6Q3, Q8CIH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 3, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi