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Protein

Beta-chimaerin

Gene

Chn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein for p21-rac.By similarity

Enzyme regulationi

In the inactive state, the N terminus protrudes into the active site of the Rho-GAP domain, sterically blocking Rac binding. Phospholipid binding to the Phorbol-ester/DAG-type zinc-finger/C1 domain triggers the cooperative dissociation of these interactions, allowing the N-terminus to move out of the active site and thereby activating the enzyme (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri78 – 12851Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-chimaerin
Alternative name(s):
Beta-chimerin
Rho GTPase-activating protein 3
Gene namesi
Name:Chn2
Synonyms:Arhgap3, Bch
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1917243. Chn2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Beta-chimaerinPRO_0000056698Add
BLAST

Proteomic databases

EPDiQ80XD1.
MaxQBiQ80XD1.
PaxDbiQ80XD1.
PRIDEiQ80XD1.

Expressioni

Gene expression databases

BgeeiQ80XD1.
CleanExiMM_CHN2.
ExpressionAtlasiQ80XD1. baseline and differential.
GenevisibleiQ80XD1. MM.

Interactioni

Protein-protein interaction databases

BioGridi213803. 1 interaction.
IntActiQ80XD1. 4 interactions.
STRINGi10090.ENSMUSP00000035908.

Structurei

3D structure databases

ProteinModelPortaliQ80XD1.
SMRiQ80XD1. Positions 75-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini141 – 332192Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri78 – 12851Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1453. Eukaryota.
ENOG410YM3I. LUCA.
GeneTreeiENSGT00840000129703.
HOGENOMiHOG000231926.
HOVERGENiHBG080489.
InParanoidiQ80XD1.
PhylomeDBiQ80XD1.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR002219. PE/DAG-bd.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80XD1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCSQELWLEN ERKCAMVRKS KPSRKRQELL AIAFGVKVGL KGGFLWSPLK
60 70 80 90 100
LFACSQISSL VRRAALTHND NHFNYEKTHN FKVHTFRGPH WCEYCANFMW
110 120 130 140 150
GLIAQGVRCS DCGLNVHKQC SKHVPNDCQP DLKRIKKVYC CDLTTLVKAH
160 170 180 190 200
NTQRPMVVDI CIREIEARGL KSEGLYRVSG FTEHIEDVKM AFDRDGEKAD
210 220 230 240 250
ISANIYPDIN IITGALKLYF RDLPIPIITY DTYSKFIEAA KISNADERLE
260 270 280 290 300
AVHEVLMLLP PAHYETLRYL MIHLKKVTMN EKDNLMNAEN LGIVFGPTLM
310 320 330
RPPEDSTLTT LHDMRYQKLI VQILIENEDV LF
Length:332
Mass (Da):38,218
Last modified:June 21, 2005 - v2
Checksum:i164E8E735053E744
GO
Isoform 2 (identifier: Q80XD1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.
     51-56: LFACSQ → MALQCR

Show »
Length:282
Mass (Da):32,541
Checksum:iC53D6D40291F4EF0
GO
Isoform 3 (identifier: Q80XD1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     56-56: Q → M

Show »
Length:277
Mass (Da):31,969
Checksum:iC4AC6534D1A8DE25
GO

Sequence cautioni

Isoform 3 : The sequence CAC08453.1 differs from that shown. Reason: Frameshift at position 302. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti138 – 1381V → G in AAH51139 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5555Missing in isoform 3. 1 PublicationVSP_014243Add
BLAST
Alternative sequencei1 – 5050Missing in isoform 2. 1 PublicationVSP_014244Add
BLAST
Alternative sequencei51 – 566LFACSQ → MALQCR in isoform 2. 1 PublicationVSP_014245
Alternative sequencei56 – 561Q → M in isoform 3. 1 PublicationVSP_014246

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ279014 mRNA. Translation: CAC08453.1. Frameshift.
AK006398 mRNA. Translation: BAB24568.1.
BC051139 mRNA. Translation: AAH51139.1.
CCDSiCCDS20154.1. [Q80XD1-1]
CCDS80535.1. [Q80XD1-2]
CCDS80536.1. [Q80XD1-3]
RefSeqiNP_001298062.1. NM_001311133.1. [Q80XD1-2]
NP_001298063.1. NM_001311134.1. [Q80XD1-3]
NP_076032.2. NM_023543.2. [Q80XD1-1]
UniGeneiMm.321147.
Mm.448157.

Genome annotation databases

EnsembliENSMUST00000067741; ENSMUSP00000066078; ENSMUSG00000004633. [Q80XD1-1]
ENSMUST00000114401; ENSMUSP00000110043; ENSMUSG00000004633. [Q80XD1-3]
ENSMUST00000146114; ENSMUSP00000114476; ENSMUSG00000004633. [Q80XD1-2]
GeneIDi69993.
KEGGimmu:69993.
UCSCiuc009bzr.2. mouse. [Q80XD1-2]
uc009bzt.2. mouse. [Q80XD1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ279014 mRNA. Translation: CAC08453.1. Frameshift.
AK006398 mRNA. Translation: BAB24568.1.
BC051139 mRNA. Translation: AAH51139.1.
CCDSiCCDS20154.1. [Q80XD1-1]
CCDS80535.1. [Q80XD1-2]
CCDS80536.1. [Q80XD1-3]
RefSeqiNP_001298062.1. NM_001311133.1. [Q80XD1-2]
NP_001298063.1. NM_001311134.1. [Q80XD1-3]
NP_076032.2. NM_023543.2. [Q80XD1-1]
UniGeneiMm.321147.
Mm.448157.

3D structure databases

ProteinModelPortaliQ80XD1.
SMRiQ80XD1. Positions 75-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213803. 1 interaction.
IntActiQ80XD1. 4 interactions.
STRINGi10090.ENSMUSP00000035908.

Proteomic databases

EPDiQ80XD1.
MaxQBiQ80XD1.
PaxDbiQ80XD1.
PRIDEiQ80XD1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067741; ENSMUSP00000066078; ENSMUSG00000004633. [Q80XD1-1]
ENSMUST00000114401; ENSMUSP00000110043; ENSMUSG00000004633. [Q80XD1-3]
ENSMUST00000146114; ENSMUSP00000114476; ENSMUSG00000004633. [Q80XD1-2]
GeneIDi69993.
KEGGimmu:69993.
UCSCiuc009bzr.2. mouse. [Q80XD1-2]
uc009bzt.2. mouse. [Q80XD1-1]

Organism-specific databases

CTDi1124.
MGIiMGI:1917243. Chn2.

Phylogenomic databases

eggNOGiKOG1453. Eukaryota.
ENOG410YM3I. LUCA.
GeneTreeiENSGT00840000129703.
HOGENOMiHOG000231926.
HOVERGENiHBG080489.
InParanoidiQ80XD1.
PhylomeDBiQ80XD1.

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Miscellaneous databases

NextBioi330757.
PROiQ80XD1.
SOURCEiSearch...

Gene expression databases

BgeeiQ80XD1.
CleanExiMM_CHN2.
ExpressionAtlasiQ80XD1. baseline and differential.
GenevisibleiQ80XD1. MM.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR002219. PE/DAG-bd.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of mouse beta chimaerin."
    Prumer A., Heinlein U.A.O.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Testis.

Entry informationi

Entry nameiCHIO_MOUSE
AccessioniPrimary (citable) accession number: Q80XD1
Secondary accession number(s): Q9D9W2, Q9ER57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: May 11, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.