Q80XD1 (CHIO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-chimaerin

Alternative name(s):
Beta-chimerin
Rho GTPase-activating protein 3

Gene names

Name:	Chn2
Synonyms:	Arhgap3, Bch

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	GTPase-activating protein for p21-rac By similarity.
Enzyme regulation	In the inactive state, the N terminus protrudes into the active site of the Rho-GAP domain, sterically blocking Rac binding. Phospholipid binding to the Phorbol-ester/DAG-type zinc-finger/C1 domain triggers the cooperative dissociation of these interactions, allowing the N-terminus to move out of the active site and thereby activating the enzyme By similarity.
Subcellular location	Membrane; Peripheral membrane protein Potential.
Sequence similarities	Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Rho-GAP domain.
Sequence caution	Isoform 3: The sequence CAC08453.1 differs from that shown. Reason: Frameshift at position 302.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q80XD1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q80XD1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.
     51-56: LFACSQ → MALQCR

Isoform 3 (identifier: Q80XD1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     56-56: Q → M

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Beta-chimaerin		PRO_0000056698
Regions
	Domain	141 – 332	192	Rho-GAP
	Zinc finger	78 – 128	51	Phorbol-ester/DAG-type
Natural variations
	Alternative sequence	1 – 55	55	Missing in isoform 3.		VSP_014243
	Alternative sequence	1 – 50	50	Missing in isoform 2.		VSP_014244
	Alternative sequence	51 – 56	6	LFACSQ → MALQCR in isoform 2.		VSP_014245
	Alternative sequence	56	1	Q → M in isoform 3.		VSP_014246
Experimental info
	Sequence conflict	138	1	V → G in AAH51139. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 164E8E735053E744	FASTA	332	38,218
	10 20 30 40 50 60 MCSQELWLEN ERKCAMVRKS KPSRKRQELL AIAFGVKVGL KGGFLWSPLK LFACSQISSL 70 80 90 100 110 120 VRRAALTHND NHFNYEKTHN FKVHTFRGPH WCEYCANFMW GLIAQGVRCS DCGLNVHKQC 130 140 150 160 170 180 SKHVPNDCQP DLKRIKKVYC CDLTTLVKAH NTQRPMVVDI CIREIEARGL KSEGLYRVSG 190 200 210 220 230 240 FTEHIEDVKM AFDRDGEKAD ISANIYPDIN IITGALKLYF RDLPIPIITY DTYSKFIEAA 250 260 270 280 290 300 KISNADERLE AVHEVLMLLP PAHYETLRYL MIHLKKVTMN EKDNLMNAEN LGIVFGPTLM 310 320 330 RPPEDSTLTT LHDMRYQKLI VQILIENEDV LF
	Isoform 2 [UniParc]. Checksum: C53D6D40291F4EF0	FASTA	282	32,541
	10 20 30 40 50 60 MALQCRISSL VRRAALTHND NHFNYEKTHN FKVHTFRGPH WCEYCANFMW GLIAQGVRCS 70 80 90 100 110 120 DCGLNVHKQC SKHVPNDCQP DLKRIKKVYC CDLTTLVKAH NTQRPMVVDI CIREIEARGL 130 140 150 160 170 180 KSEGLYRVSG FTEHIEDVKM AFDRDGEKAD ISANIYPDIN IITGALKLYF RDLPIPIITY 190 200 210 220 230 240 DTYSKFIEAA KISNADERLE AVHEVLMLLP PAHYETLRYL MIHLKKVTMN EKDNLMNAEN 250 260 270 280 LGIVFGPTLM RPPEDSTLTT LHDMRYQKLI VQILIENEDV LF
	Isoform 3 [UniParc]. Checksum: C4AC6534D1A8DE25	FASTA	277	31,969
	10 20 30 40 50 60 MISSLVRRAA LTHNDNHFNY EKTHNFKVHT FRGPHWCEYC ANFMWGLIAQ GVRCSDCGLN 70 80 90 100 110 120 VHKQCSKHVP NDCQPDLKRI KKVYCCDLTT LVKAHNTQRP MVVDICIREI EARGLKSEGL 130 140 150 160 170 180 YRVSGFTEHI EDVKMAFDRD GEKADISANI YPDINIITGA LKLYFRDLPI PIITYDTYSK 190 200 210 220 230 240 FIEAAKISNA DERLEAVHEV LMLLPPAHYE TLRYLMIHLK KVTMNEKDNL MNAENLGIVF 250 260 270 GPTLMRPPED STLTTLHDMR YQKLIVQILI ENEDVLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of mouse beta chimaerin." Prumer A., Heinlein U.A.O. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Testis.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ279014 mRNA. Translation: CAC08453.1. Frameshift. AK006398 mRNA. Translation: BAB24568.1. BC051139 mRNA. Translation: AAH51139.1.
IPI	IPI00113201. IPI00608049. IPI00653679.
RefSeq	NP_076032.2. NM_023543.2.
UniGene	Mm.321147. Mm.448157.
3D structure databases
ProteinModelPortal	Q80XD1.
SMR	Q80XD1. Positions 75-332.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-5087994.
Proteomic databases
PaxDb	Q80XD1.
PRIDE	Q80XD1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000067741; ENSMUSP00000066078; ENSMUSG00000004633. ENSMUST00000114401; ENSMUSP00000110043; ENSMUSG00000004633. ENSMUST00000146114; ENSMUSP00000114476; ENSMUSG00000004633.
GeneID	69993.
KEGG	mmu:69993.
UCSC	uc009bzr.2. mouse. uc009bzt.2. mouse.
Organism-specific databases
CTD	1124.
MGI	MGI:1917243. Chn2.
Phylogenomic databases
eggNOG	NOG295484.
GeneTree	ENSGT00700000104152.
HOGENOM	HOG000231926.
HOVERGEN	HBG080489.
Gene expression databases
ArrayExpress	Q80XD1.
Bgee	Q80XD1.
CleanEx	MM_CHN2.
Genevestigator	Q80XD1.
GermOnline	ENSMUSG00000004633. Mus musculus.
Family and domain databases
Gene3D	1.10.555.10. 1 hit.
InterPro	IPR020454. DAG/PE-bd. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. [Graphical view]
Pfam	PF00130. C1_1. 1 hit. PF00620. RhoGAP. 1 hit. [Graphical view]
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 1 hit. SM00324. RhoGAP. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50238. RHOGAP. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CHN2. mouse.
NextBio	330757.
SOURCE	Search...

Entry information

Entry name

CHIO_MOUSE

Accession

Primary (citable) accession number: Q80XD1
Secondary accession number(s): Q9D9W2, Q9ER57

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	April 3, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program