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Protein

Beta-chimaerin

Gene

Chn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein for p21-rac.By similarity

Enzyme regulationi

In the inactive state, the N terminus protrudes into the active site of the Rho-GAP domain, sterically blocking Rac binding. Phospholipid binding to the Phorbol-ester/DAG-type zinc-finger/C1 domain triggers the cooperative dissociation of these interactions, allowing the N-terminus to move out of the active site and thereby activating the enzyme (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri78 – 128Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-chimaerin
Alternative name(s):
Beta-chimerin
Rho GTPase-activating protein 3
Gene namesi
Name:Chn2
Synonyms:Arhgap3, Bch
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1917243. Chn2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000566981 – 332Beta-chimaerinAdd BLAST332

Proteomic databases

PaxDbiQ80XD1.
PRIDEiQ80XD1.

PTM databases

PhosphoSitePlusiQ80XD1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004633.
CleanExiMM_CHN2.
ExpressionAtlasiQ80XD1. baseline and differential.
GenevisibleiQ80XD1. MM.

Interactioni

Protein-protein interaction databases

BioGridi213803. 1 interactor.
IntActiQ80XD1. 4 interactors.
STRINGi10090.ENSMUSP00000035908.

Structurei

3D structure databases

ProteinModelPortaliQ80XD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 332Rho-GAPPROSITE-ProRule annotationAdd BLAST192

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri78 – 128Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1453. Eukaryota.
ENOG410YM3I. LUCA.
GeneTreeiENSGT00840000129684.
HOGENOMiHOG000231926.
HOVERGENiHBG080489.
InParanoidiQ80XD1.
KOiK20630.
PhylomeDBiQ80XD1.

Family and domain databases

CDDicd00029. C1. 1 hit.
Gene3Di1.10.555.10. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR002219. PE/DAG-bd.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80XD1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCSQELWLEN ERKCAMVRKS KPSRKRQELL AIAFGVKVGL KGGFLWSPLK
60 70 80 90 100
LFACSQISSL VRRAALTHND NHFNYEKTHN FKVHTFRGPH WCEYCANFMW
110 120 130 140 150
GLIAQGVRCS DCGLNVHKQC SKHVPNDCQP DLKRIKKVYC CDLTTLVKAH
160 170 180 190 200
NTQRPMVVDI CIREIEARGL KSEGLYRVSG FTEHIEDVKM AFDRDGEKAD
210 220 230 240 250
ISANIYPDIN IITGALKLYF RDLPIPIITY DTYSKFIEAA KISNADERLE
260 270 280 290 300
AVHEVLMLLP PAHYETLRYL MIHLKKVTMN EKDNLMNAEN LGIVFGPTLM
310 320 330
RPPEDSTLTT LHDMRYQKLI VQILIENEDV LF
Length:332
Mass (Da):38,218
Last modified:June 21, 2005 - v2
Checksum:i164E8E735053E744
GO
Isoform 2 (identifier: Q80XD1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.
     51-56: LFACSQ → MALQCR

Show »
Length:282
Mass (Da):32,541
Checksum:iC53D6D40291F4EF0
GO
Isoform 3 (identifier: Q80XD1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     56-56: Q → M

Show »
Length:277
Mass (Da):31,969
Checksum:iC4AC6534D1A8DE25
GO

Sequence cautioni

Isoform 3 : The sequence CAC08453 differs from that shown. Reason: Frameshift at position 302.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138V → G in AAH51139 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0142431 – 55Missing in isoform 3. 1 PublicationAdd BLAST55
Alternative sequenceiVSP_0142441 – 50Missing in isoform 2. 1 PublicationAdd BLAST50
Alternative sequenceiVSP_01424551 – 56LFACSQ → MALQCR in isoform 2. 1 Publication6
Alternative sequenceiVSP_01424656Q → M in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ279014 mRNA. Translation: CAC08453.1. Frameshift.
AK006398 mRNA. Translation: BAB24568.1.
BC051139 mRNA. Translation: AAH51139.1.
CCDSiCCDS20154.1. [Q80XD1-1]
CCDS80535.1. [Q80XD1-2]
CCDS80536.1. [Q80XD1-3]
RefSeqiNP_001298062.1. NM_001311133.1. [Q80XD1-2]
NP_001298063.1. NM_001311134.1. [Q80XD1-3]
NP_076032.2. NM_023543.2. [Q80XD1-1]
UniGeneiMm.321147.
Mm.448157.

Genome annotation databases

EnsembliENSMUST00000067741; ENSMUSP00000066078; ENSMUSG00000004633. [Q80XD1-1]
ENSMUST00000114401; ENSMUSP00000110043; ENSMUSG00000004633. [Q80XD1-3]
ENSMUST00000146114; ENSMUSP00000114476; ENSMUSG00000004633. [Q80XD1-2]
GeneIDi69993.
KEGGimmu:69993.
UCSCiuc009bzr.2. mouse. [Q80XD1-2]
uc009bzt.2. mouse. [Q80XD1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ279014 mRNA. Translation: CAC08453.1. Frameshift.
AK006398 mRNA. Translation: BAB24568.1.
BC051139 mRNA. Translation: AAH51139.1.
CCDSiCCDS20154.1. [Q80XD1-1]
CCDS80535.1. [Q80XD1-2]
CCDS80536.1. [Q80XD1-3]
RefSeqiNP_001298062.1. NM_001311133.1. [Q80XD1-2]
NP_001298063.1. NM_001311134.1. [Q80XD1-3]
NP_076032.2. NM_023543.2. [Q80XD1-1]
UniGeneiMm.321147.
Mm.448157.

3D structure databases

ProteinModelPortaliQ80XD1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213803. 1 interactor.
IntActiQ80XD1. 4 interactors.
STRINGi10090.ENSMUSP00000035908.

PTM databases

PhosphoSitePlusiQ80XD1.

Proteomic databases

PaxDbiQ80XD1.
PRIDEiQ80XD1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067741; ENSMUSP00000066078; ENSMUSG00000004633. [Q80XD1-1]
ENSMUST00000114401; ENSMUSP00000110043; ENSMUSG00000004633. [Q80XD1-3]
ENSMUST00000146114; ENSMUSP00000114476; ENSMUSG00000004633. [Q80XD1-2]
GeneIDi69993.
KEGGimmu:69993.
UCSCiuc009bzr.2. mouse. [Q80XD1-2]
uc009bzt.2. mouse. [Q80XD1-1]

Organism-specific databases

CTDi1124.
MGIiMGI:1917243. Chn2.

Phylogenomic databases

eggNOGiKOG1453. Eukaryota.
ENOG410YM3I. LUCA.
GeneTreeiENSGT00840000129684.
HOGENOMiHOG000231926.
HOVERGENiHBG080489.
InParanoidiQ80XD1.
KOiK20630.
PhylomeDBiQ80XD1.

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Miscellaneous databases

PROiQ80XD1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004633.
CleanExiMM_CHN2.
ExpressionAtlasiQ80XD1. baseline and differential.
GenevisibleiQ80XD1. MM.

Family and domain databases

CDDicd00029. C1. 1 hit.
Gene3Di1.10.555.10. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR002219. PE/DAG-bd.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHIO_MOUSE
AccessioniPrimary (citable) accession number: Q80XD1
Secondary accession number(s): Q9D9W2, Q9ER57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: November 30, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.