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Protein

USP6 N-terminal-like protein

Gene

Usp6nl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a GTPase-activating protein for RAB5A and RAB43. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for structural integrity of the Golgi complex (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
USP6 N-terminal-like protein
Gene namesi
Name:Usp6nl
Synonyms:Kiaa0019
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2138893. Usp6nl.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002080571 – 819USP6 N-terminal-like proteinAdd BLAST819

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei389PhosphoserineBy similarity1
Modified residuei394PhosphoserineBy similarity1
Modified residuei398PhosphoserineBy similarity1
Modified residuei544PhosphoserineCombined sources1
Modified residuei547PhosphoserineCombined sources1
Modified residuei574PhosphoserineBy similarity1
Modified residuei631PhosphoserineBy similarity1
Modified residuei644PhosphoserineBy similarity1
Modified residuei648PhosphoserineBy similarity1
Modified residuei665PhosphoserineCombined sources1
Modified residuei669PhosphoserineBy similarity1
Modified residuei704PhosphoserineBy similarity1
Modified residuei717PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ80XC3.
PRIDEiQ80XC3.

PTM databases

iPTMnetiQ80XC3.
PhosphoSitePlusiQ80XC3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039046.
CleanExiMM_USP6NL.
GenevisibleiQ80XC3. MM.

Interactioni

Subunit structurei

Interacts with EPS8.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043178.

Structurei

3D structure databases

ProteinModelPortaliQ80XC3.
SMRiQ80XC3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 292Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST193

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi567 – 659Pro-richAdd BLAST93

Sequence similaritiesi

Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1102. Eukaryota.
COG5210. LUCA.
GeneTreeiENSGT00860000133698.
HOGENOMiHOG000139916.
HOVERGENiHBG059159.
InParanoidiQ80XC3.
KOiK20133.
OMAiFVPKWNK.
OrthoDBiEOG091G04OG.
PhylomeDBiQ80XC3.
TreeFamiTF318099.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80XC3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSDQDVALK LAQERAEIVA KYDRGREGAE IEPWEDADYL VYKVTDRFGF
60 70 80 90 100
LHEEELPYHN AAADRQKQLE IERTSKWLKM LKKWERYKNT EKFHRRIYKG
110 120 130 140 150
IPLQLRGEVW ALLLEIPKMK EETRDLYSKL KHRARGCSPD IRQIDLDVNR
160 170 180 190 200
TFRDHIMFRD RYGVKQQSLF HVLAAYSIYN TEVGYCQGMS QITALLLMYM
210 220 230 240 250
NEEDAFWALV KLFSGPKHAM HGFFVQGFPK LLRFQEHHEK ILNKFLSKLK
260 270 280 290 300
QHLDSQEIYT SFYTMKWFFQ CFLDRTPFRL NLRIWDIYIF EGERVLTAMS
310 320 330 340 350
YTILKLHKKH LMKLSMEELV EFLQETLAKD FFFEDDFVIE QLQVSMAELK
360 370 380 390 400
RAKLDLPEPG KEDEYPKKPL GQLPPESACV NHLSNGQRSV GRPSPKTSSR
410 420 430 440 450
REDGSPRKNH EHSPVHHSRN GTPERAGQSR RKSVDEGSKN LKHEAESQRK
460 470 480 490 500
PSPGMQDSSR HYNHAAANQN SNAISNVRKE FMPKWRKPSD ASAIERTTKY
510 520 530 540 550
AVEGKSHSAL PALPVAIPGS AETRLPNSRQ KMKALDGGEG KRGSNASQYD
560 570 580 590 600
NVPGGESEHG ASAEEGPERT HPHSPRKHPE PSPSPPKVPN KFTFKVQPPS
610 620 630 640 650
HVRYPPQLPE EDHRAAYPPS YSNPPVYHGN SPKHVPTAHS GFVSTQISPR
660 670 680 690 700
PQINPSRRPY GSSLSVDTSP EKAYSRPTPV VLPSSRIEVL PIDMGARGYG
710 720 730 740 750
SSGSPKNGQF ILPPVDYLPE NRKWSEVSYT YRPEMHGQSW TRDAHRSHLS
760 770 780 790 800
NLPNYAAFQH IPFQAHGLPE VSVDSPVRYK MSAAVEDASP PGYPYAGPSP
810
SAHHYRNGEG LSVQESVLL
Length:819
Mass (Da):93,574
Last modified:July 30, 2004 - v2
Checksum:iAD6B72BB42A00B54
GO
Isoform 2 (identifier: Q80XC3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MN → MIQVLQLVKELVTPSRQKAATAKED

Note: No experimental confirmation available.
Show »
Length:842
Mass (Da):96,108
Checksum:i0E2860788E5ACB14
GO

Sequence cautioni

The sequence BAC97847 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BC051184 differs from that shown. Reason: Frameshift at position 671.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti446E → G in BAE33027 (PubMed:16141072).Curated1
Sequence conflicti765A → V in BAE33027 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0111531 – 2MN → MIQVLQLVKELVTPSRQKAA TAKED in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129037 mRNA. Translation: BAC97847.1. Different initiation.
AK155073 mRNA. Translation: BAE33027.1.
AL845275, AL845515 Genomic DNA. Translation: CAM13701.1.
AL845275, AL845515 Genomic DNA. Translation: CAM13702.1.
AL845515, AL845275 Genomic DNA. Translation: CAM18814.1.
AL845515, AL845275 Genomic DNA. Translation: CAM18815.1.
BC051184 mRNA. No translation available.
CCDSiCCDS38043.1. [Q80XC3-1]
CCDS38044.1. [Q80XC3-2]
RefSeqiNP_001074017.1. NM_001080548.1. [Q80XC3-1]
NP_852064.2. NM_181399.3. [Q80XC3-2]
XP_006497632.1. XM_006497569.3. [Q80XC3-1]
UniGeneiMm.40673.

Genome annotation databases

EnsembliENSMUST00000042503; ENSMUSP00000043178; ENSMUSG00000039046. [Q80XC3-2]
ENSMUST00000114937; ENSMUSP00000110587; ENSMUSG00000039046. [Q80XC3-1]
GeneIDi98910.
KEGGimmu:98910.
UCSCiuc008igk.1. mouse. [Q80XC3-1]
uc008igl.1. mouse. [Q80XC3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129037 mRNA. Translation: BAC97847.1. Different initiation.
AK155073 mRNA. Translation: BAE33027.1.
AL845275, AL845515 Genomic DNA. Translation: CAM13701.1.
AL845275, AL845515 Genomic DNA. Translation: CAM13702.1.
AL845515, AL845275 Genomic DNA. Translation: CAM18814.1.
AL845515, AL845275 Genomic DNA. Translation: CAM18815.1.
BC051184 mRNA. No translation available.
CCDSiCCDS38043.1. [Q80XC3-1]
CCDS38044.1. [Q80XC3-2]
RefSeqiNP_001074017.1. NM_001080548.1. [Q80XC3-1]
NP_852064.2. NM_181399.3. [Q80XC3-2]
XP_006497632.1. XM_006497569.3. [Q80XC3-1]
UniGeneiMm.40673.

3D structure databases

ProteinModelPortaliQ80XC3.
SMRiQ80XC3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043178.

PTM databases

iPTMnetiQ80XC3.
PhosphoSitePlusiQ80XC3.

Proteomic databases

PaxDbiQ80XC3.
PRIDEiQ80XC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042503; ENSMUSP00000043178; ENSMUSG00000039046. [Q80XC3-2]
ENSMUST00000114937; ENSMUSP00000110587; ENSMUSG00000039046. [Q80XC3-1]
GeneIDi98910.
KEGGimmu:98910.
UCSCiuc008igk.1. mouse. [Q80XC3-1]
uc008igl.1. mouse. [Q80XC3-2]

Organism-specific databases

CTDi9712.
MGIiMGI:2138893. Usp6nl.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1102. Eukaryota.
COG5210. LUCA.
GeneTreeiENSGT00860000133698.
HOGENOMiHOG000139916.
HOVERGENiHBG059159.
InParanoidiQ80XC3.
KOiK20133.
OMAiFVPKWNK.
OrthoDBiEOG091G04OG.
PhylomeDBiQ80XC3.
TreeFamiTF318099.

Enzyme and pathway databases

ReactomeiR-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

ChiTaRSiUsp6nl. mouse.
PROiQ80XC3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039046.
CleanExiMM_USP6NL.
GenevisibleiQ80XC3. MM.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUS6NL_MOUSE
AccessioniPrimary (citable) accession number: Q80XC3
Secondary accession number(s): A2AR45
, A2AR46, Q3U2W3, Q6ZQK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 30, 2004
Last sequence update: July 30, 2004
Last modified: November 30, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.