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Protein

tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A

Gene

Trmt61a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Catalytic subunit of mRNA N1-methyltransferase complex, which mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.By similarity

Catalytic activityi

S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N1-methyladenine(58) in tRNA.PROSITE-ProRule annotation
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N1-methyladenine in mRNA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei135S-adenosyl-L-methioninePROSITE-ProRule annotationBy similarity1
Binding sitei140S-adenosyl-L-methionineBy similarity1
Binding sitei181S-adenosyl-L-methioninePROSITE-ProRule annotationBy similarity1
Binding sitei279SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A (EC:2.1.1.220By similarity)
Alternative name(s):
mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A (EC:2.1.1.-By similarity)
tRNA(m1A58)-methyltransferase subunit TRMT61A
Short name:
tRNA(m1A58)MTase subunit TRMT61A
Gene namesi
Name:Trmt61a
Synonyms:Trm61
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2443487 Trmt61a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002330952 – 290tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61AAdd BLAST289

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei264PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ80XC2
MaxQBiQ80XC2
PaxDbiQ80XC2
PeptideAtlasiQ80XC2
PRIDEiQ80XC2

PTM databases

iPTMnetiQ80XC2
PhosphoSitePlusiQ80XC2

Expressioni

Gene expression databases

BgeeiENSMUSG00000060950
CleanExiMM_6720458F09RIK
ExpressionAtlasiQ80XC2 baseline and differential
GenevisibleiQ80XC2 MM

Interactioni

Subunit structurei

Heterotetramer; composed of two copies of TRMT6 and two copies of TRMT61A.By similarity

Protein-protein interaction databases

IntActiQ80XC2, 2 interactors
MINTiQ80XC2
STRINGi10090.ENSMUSP00000082011

Structurei

3D structure databases

ProteinModelPortaliQ80XC2
SMRiQ80XC2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 22SubstrateBy similarity3
Regioni35 – 42SubstrateBy similarity8
Regioni64 – 65SubstrateBy similarity2
Regioni85 – 89SubstrateBy similarity5
Regioni110 – 117SubstrateBy similarity8
Regioni114 – 116S-adenosyl-L-methionine bindingBy similarity3
Regioni163 – 164S-adenosyl-L-methionine bindingBy similarity2
Regioni180 – 183SubstrateBy similarity4
Regioni205 – 212SubstrateBy similarity8

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2915 Eukaryota
COG2519 LUCA
GeneTreeiENSGT00510000048063
HOGENOMiHOG000173282
HOVERGENiHBG061059
InParanoidiQ80XC2
KOiK07442
OMAiVVYPKDA
OrthoDBiEOG091G0BHV
PhylomeDBiQ80XC2
TreeFamiTF315053

Family and domain databases

InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR014816 tRNA_MeTrfase_Gcd14
PANTHERiPTHR12133 PTHR12133, 1 hit
PfamiView protein in Pfam
PF08704 GCD14, 1 hit
PIRSFiPIRSF017269 GCD14, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51620 SAM_TRM61, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80XC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFVAYEELI KEGDTAILSL GHGSMVAVRV QRGAQTQTRH GVLRHSVDLI
60 70 80 90 100
GRPFGSKVIC SRGGWVYVLH PTPELWTVNL PHRTQILYST DIALITMMLE
110 120 130 140 150
LRPGSVVCES GTGSGSVSHA IIRSVAPTGH LHTVEFHQQR ADKAREEFQE
160 170 180 190 200
HRLSQWVTVH TQDVCCSGFG VVHVADAVFL DIPSPWEAVG HAWDALKVEG
210 220 230 240 250
GRFCSFSPCI EQVQRTCQAL AAHGFTELST LEVLPQVYNV RTVSLPLPDL
260 270 280 290
GANNLETNMG SDASPFRSGT PMKETVGHTG YLTFATKTPG
Length:290
Mass (Da):31,639
Last modified:June 1, 2003 - v1
Checksum:i7F9F291EE94E6FFC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70H → R in BAC33565 (PubMed:16141072).Curated1
Sequence conflicti224G → D in BAC28042 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049138 mRNA Translation: BAC33565.1
AK032824 mRNA Translation: BAC28042.1
BC051186 mRNA Translation: AAH51186.1
CCDSiCCDS26184.1
RefSeqiNP_001093262.1, NM_001099792.1
NP_001093263.1, NM_001099793.1
NP_796348.2, NM_177374.4
UniGeneiMm.425747

Genome annotation databases

EnsembliENSMUST00000084947; ENSMUSP00000082011; ENSMUSG00000060950
ENSMUST00000168338; ENSMUSP00000133128; ENSMUSG00000060950
GeneIDi328162
KEGGimmu:328162
UCSCiuc007pdo.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTRM61_MOUSE
AccessioniPrimary (citable) accession number: Q80XC2
Secondary accession number(s): Q8BMD4, Q8BX33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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