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Q80X82

- SYMPK_MOUSE

UniProt

Q80X82 - SYMPK_MOUSE

Protein

Symplekin

Gene

Sympk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity By similarity.By similarity

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. mRNA polyadenylation Source: UniProtKB
    3. positive regulation of protein dephosphorylation Source: UniProtKB

    Keywords - Biological processi

    Cell adhesion, mRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Symplekin
    Gene namesi
    Name:Sympk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1915438. Sympk.

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity. Cell junctiontight junction. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cell junction By similarity. Nucleusnucleoplasm By similarity
    Note: Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without TJ). Nuclear bodies of heat-stressed cells By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. cytoskeleton Source: UniProtKB-SubCell
    3. nucleoplasm Source: UniProtKB-SubCell
    4. plasma membrane Source: UniProtKB-SubCell
    5. tight junction Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus, Tight junction

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12841284SymplekinPRO_0000072386Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei494 – 4941PhosphoserineBy similarity
    Modified residuei1256 – 12561PhosphoserineBy similarity
    Modified residuei1270 – 12701PhosphothreonineBy similarity
    Modified residuei1272 – 12721PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ80X82.
    PaxDbiQ80X82.
    PRIDEiQ80X82.

    PTM databases

    PhosphoSiteiQ80X82.

    Expressioni

    Gene expression databases

    ArrayExpressiQ80X82.
    BgeeiQ80X82.
    CleanExiMM_SYMPK.
    GenevestigatoriQ80X82.

    Interactioni

    Subunit structurei

    Found in a heat-sensitive complex at least composed of several cleavage and polyadenylation specific and cleavage stimulation factors. Interacts with CPSF2, CPSF3 and CSTF2. Interacts with HSF1 in heat-stressed cells. Interacts with SSU72 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ80X82. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80X82.
    SMRiQ80X82. Positions 30-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati31 – 6434HEAT 1Add
    BLAST
    Repeati67 – 10135HEAT 2Add
    BLAST
    Repeati104 – 14643HEAT 3Add
    BLAST
    Repeati153 – 19240HEAT 4Add
    BLAST
    Repeati227 – 26640HEAT 5Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 124124Interaction with HSF1By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi345 – 36016Nuclear localization signalSequence AnalysisAdd
    BLAST

    Domaini

    The HEAT repeats have been determined based on 3D-structure analysis of the D.melanogaster ortholog and are not detected by sequence-based prediction programs.

    Sequence similaritiesi

    Belongs to the Symplekin family.Curated
    Contains 5 HEAT repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG273943.
    HOGENOMiHOG000046745.
    HOVERGENiHBG062441.
    InParanoidiQ80X82.
    PhylomeDBiQ80X82.

    Family and domain databases

    Gene3Di1.25.10.10. 2 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR021850. Symplekin/Pta1.
    IPR022075. Symplekin_C.
    [Graphical view]
    PANTHERiPTHR15245:SF20. PTHR15245:SF20. 1 hit.
    PfamiPF12295. Symplekin_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Q80X82-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASSSGDSVT RRSVASQFFT QEEGPSIDGM TTSERVVDLL NQAALITNDS     50
    KITVLKQVQE LIINKDPTLL DNFLDEIIAF QADKSIEVRK FVIGFIEEAC 100
    KRDIELLLKL IANLNMLLRD ENVNVVKKAI LTMTQLYKVA LQWMVKSRVI 150
    SDLQEACWDM VSSMAGEIIL LLDSDNDGIR THAIKFVEGL IVTLSPRMAD 200
    SEVPRRQEHD ISLDRIPRDH PYIQYNVLWE EGKAAVEQLL KFMVHPAISS 250
    INLTTALGSL ANIARQRPMF MSEVIQAYET LHANLPPTLA KSQVSSVRKN 300
    LKLHLLSVLK HPASLEFQAQ ITTLLVDLGT PQAEIARNMP SSKDSRKRPR 350
    DDTDSTLKKM KLEPNLGEDD EDKDLEPGPS GTSKASAQIS GQSDTDITAE 400
    FLQPLLTPDN VANLVLISMV YLPETMPASF QAIYTPVESA GTEAQIKHLA 450
    RLMATQMTAA GLGPGVEQTK QCKEEPKEEK VVKPESVLIK RRLSVQGQAI 500
    SVVGSQSTMS PLEEEVPQAK RRPEPIIPVT QPRLAGAGGR KKIFRLSDVL 550
    KPLTDAQVEA MKLGAVKRIL RAEKAVACSG AAQVRIKILA SLVTQFDSGF 600
    KAEVLSFILE DVRARLDLAF AWLYQEYNAY LAAGTSGTLD KYEDCLICLL 650
    SGLQEKPDQK DGIFTKVVLE APLITESALE VIRKYCEDES RAYLGMSTLG 700
    DLIFKRPSRQ FQYLHVLLDL SSHEKDRVRS QALLFIKRMY EKEQLREYVE 750
    KFALNYLQLL VHPNPPSVLF GADKDTEVAA PWTEETVKQC LYLYLALLPQ 800
    NHKLIHELAA VYTEAIADIK RTVLRVIEQP IRGMGMNSPE LLLLVENCPK 850
    GAETLVTRCL HSLTDKVPPS PELVKRVRDL YHKRLPDVRF LIPVLNGLEK 900
    KEVIQALPKL IKLNPIVVKE VFNRLLGTQH GEGNSALSPL NPGELLIALH 950
    NIDSVKCDMK SIIKATNLCF AERNVYTSEV LAVVMQQLME QSPLPMLLMR 1000
    TVIQSLTMYP RLGGFVMNIL ARLIMKQVWK YPKVWEGFIK CCQRTKPQSF 1050
    QVILQLPPQQ LGAVFDKCPE LREPLLAHVR SFTPHQQAHI PNSIMTILEA 1100
    TGKQEPEVKE APSGPLEEDD LEPLALALAP APAPAPAPAP APRPPQDLIG 1150
    LRLAQEKALK RQLEEEQKQK PTGIGAPAAC VSSTPSVPAA ARAGPTPAEE 1200
    VMEYREEGPE CETPAIFISM DDDSGLAETT LLDSSLEGPL PKEAAAVGSS 1250
    SKDERSPQNL SHAVEEALKT SSPETREPES KGNS 1284
    Length:1,284
    Mass (Da):142,284
    Last modified:June 1, 2003 - v1
    Checksum:i983FA5300374DC5B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC049852 mRNA. Translation: AAH49852.1.
    UniGeneiMm.130902.

    Genome annotation databases

    UCSCiuc009fkg.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC049852 mRNA. Translation: AAH49852.1 .
    UniGenei Mm.130902.

    3D structure databases

    ProteinModelPortali Q80X82.
    SMRi Q80X82. Positions 30-340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q80X82. 1 interaction.

    PTM databases

    PhosphoSitei Q80X82.

    Proteomic databases

    MaxQBi Q80X82.
    PaxDbi Q80X82.
    PRIDEi Q80X82.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi uc009fkg.2. mouse.

    Organism-specific databases

    MGIi MGI:1915438. Sympk.

    Phylogenomic databases

    eggNOGi NOG273943.
    HOGENOMi HOG000046745.
    HOVERGENi HBG062441.
    InParanoidi Q80X82.
    PhylomeDBi Q80X82.

    Miscellaneous databases

    PROi Q80X82.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q80X82.
    Bgeei Q80X82.
    CleanExi MM_SYMPK.
    Genevestigatori Q80X82.

    Family and domain databases

    Gene3Di 1.25.10.10. 2 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR021850. Symplekin/Pta1.
    IPR022075. Symplekin_C.
    [Graphical view ]
    PANTHERi PTHR15245:SF20. PTHR15245:SF20. 1 hit.
    Pfami PF12295. Symplekin_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiSYMPK_MOUSE
    AccessioniPrimary (citable) accession number: Q80X82
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3