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Protein

E3 ubiquitin-protein ligase TRIM69

Gene

Trim69

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have E3 ubiquitin-protein ligase activity. May play a role in apoptosis.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 8342RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM69 (EC:6.3.2.-)
Alternative name(s):
RING finger B-box coiled-coil transcription factor
RING finger protein 36
Testis-specific RING finger protein
Tripartite motif-containing protein 69
Gene namesi
Name:Trim69
Synonyms:Rnf36, Trif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1918178. Trim69.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500E3 ubiquitin-protein ligase TRIM69PRO_0000278237Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei342 – 3421PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated. Phosphorylation is necessary for nuclear localization.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80X56.
PRIDEiQ80X56.

PTM databases

iPTMnetiQ80X56.
PhosphoSiteiQ80X56.

Expressioni

Tissue specificityi

Expressed in spermatid.1 Publication

Gene expression databases

BgeeiENSMUSG00000033368.
CleanExiMM_TRIM69.
GenevisibleiQ80X56. MM.

Interactioni

Subunit structurei

Interacts with PML.1 Publication

Protein-protein interaction databases

BioGridi214351. 36 interactions.
IntActiQ80X56. 40 interactions.
STRINGi10090.ENSMUSP00000047627.

Structurei

3D structure databases

ProteinModelPortaliQ80X56.
SMRiQ80X56. Positions 29-105, 115-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini306 – 500195B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 153153Necessary for nuclear localizationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili217 – 25640Sequence analysisAdd
BLAST

Domaini

The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity and for nuclear localization and aggregation.By similarity

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 8342RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFB. Eukaryota.
ENOG410XWXM. LUCA.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000234133.
HOVERGENiHBG001357.
InParanoidiQ80X56.
KOiK12034.
OMAiMICFQCK.
OrthoDBiEOG091G05W2.
PhylomeDBiQ80X56.
TreeFamiTF342569.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80X56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVSSRPPSN FDPGNYVEMS DPTTTHLPSK VVIQDLTTEL HCPLCNDWFR
60 70 80 90 100
DPLMLTCGHN FCQDCIQSFW KVHSKETFCP DCKMLCQYSN CTFNLVLEKL
110 120 130 140 150
VEKIKKLPLL KGHPQCPEHG ENLKLFSKPE GKMICFQCKD ARLSMGQSKD
160 170 180 190 200
FLQISEAVRF FTEELAIYQS QLQTTLKELQ SLRTIQKDAI SAYKDNKIQL
210 220 230 240 250
QQNLSLEFLK LHQFLHNKEK DILNDLRDEG KLLNEEMEVN LNQIQEQCLV
260 270 280 290 300
AKDMLATIQA RMEQQNSFDF LTDITKLIES MEKGMKTIVP RQLIAKKLSL
310 320 330 340 350
GRFKGPIQYI IWKEMQAILS PGPSQLTLDP KTAHPNLVLS KSQTSVCHCD
360 370 380 390 400
VKQVMPDDPE RFDSSVAVLG SKGFTSGKWY WEIEVGKKTK WTIGVVRESI
410 420 430 440 450
IRKGSCPLTP EQGFWLLRLR NQTDLKALDL PSRSLTLGDL RRVGVYLDYE
460 470 480 490 500
GGQVSFYNAT TMTHLYTFSS VFQEKLFPYL CPCLNDGGEN KEPLHIVHPQ
Length:500
Mass (Da):57,312
Last modified:June 1, 2003 - v1
Checksum:i607B0CEB02213754
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171P → A in AAL41031 (PubMed:11578878).Curated
Sequence conflicti355 – 3551M → L in AAL41031 (PubMed:11578878).Curated
Sequence conflicti362 – 3621F → L in AAL41031 (PubMed:11578878).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334958 mRNA. Translation: AAL41031.1.
AL845457 Genomic DNA. Translation: CAM19390.1.
AL845457 Genomic DNA. Translation: CAM21784.1.
BC050815 mRNA. Translation: AAH50815.1.
CCDSiCCDS16655.1.
RefSeqiNP_536771.2. NM_080510.2.
UniGeneiMm.379188.

Genome annotation databases

EnsembliENSMUST00000036089; ENSMUSP00000047627; ENSMUSG00000033368.
GeneIDi70928.
KEGGimmu:70928.
UCSCiuc008mah.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334958 mRNA. Translation: AAL41031.1.
AL845457 Genomic DNA. Translation: CAM19390.1.
AL845457 Genomic DNA. Translation: CAM21784.1.
BC050815 mRNA. Translation: AAH50815.1.
CCDSiCCDS16655.1.
RefSeqiNP_536771.2. NM_080510.2.
UniGeneiMm.379188.

3D structure databases

ProteinModelPortaliQ80X56.
SMRiQ80X56. Positions 29-105, 115-496.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214351. 36 interactions.
IntActiQ80X56. 40 interactions.
STRINGi10090.ENSMUSP00000047627.

PTM databases

iPTMnetiQ80X56.
PhosphoSiteiQ80X56.

Proteomic databases

PaxDbiQ80X56.
PRIDEiQ80X56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036089; ENSMUSP00000047627; ENSMUSG00000033368.
GeneIDi70928.
KEGGimmu:70928.
UCSCiuc008mah.1. mouse.

Organism-specific databases

CTDi140691.
MGIiMGI:1918178. Trim69.

Phylogenomic databases

eggNOGiENOG410ITFB. Eukaryota.
ENOG410XWXM. LUCA.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000234133.
HOVERGENiHBG001357.
InParanoidiQ80X56.
KOiK12034.
OMAiMICFQCK.
OrthoDBiEOG091G05W2.
PhylomeDBiQ80X56.
TreeFamiTF342569.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiQ80X56.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033368.
CleanExiMM_TRIM69.
GenevisibleiQ80X56. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRI69_MOUSE
AccessioniPrimary (citable) accession number: Q80X56
Secondary accession number(s): Q8VHZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.