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Q80X41

- VRK1_MOUSE

UniProt

Q80X41 - VRK1_MOUSE

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Protein

Serine/threonine-protein kinase VRK1

Gene

Vrk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Active in presence of Mn2+, Mg2+ and Zn2+, but is not functional with Ca2+ or Cu2+. Has a higher affinity for Mn2+ than for Mg2+. RAN inhibits its autophosphorylation and its ability to phosphorylate histone H3 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711ATPPROSITE-ProRule annotation
Active sitei177 – 1771Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 519ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. histone kinase activity (H3-S10 specific) Source: Ensembl
  3. histone kinase activity (H3-T3 specific) Source: Ensembl
  4. protein serine/threonine kinase activity Source: MGI

GO - Biological processi

  1. Golgi disassembly Source: UniProtKB
  2. mitotic nuclear division Source: UniProtKB-KW
  3. protein autophosphorylation Source: MGI
  4. protein phosphorylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_210180. Initiation of Nuclear Envelope Reformation.
REACT_221677. Clearance of Nuclear Envelope Membranes from Chromatin.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase VRK1 (EC:2.7.11.1)
Alternative name(s):
Serine/threonine-protein kinase 51PK
Vaccinia-related kinase 1
Gene namesi
Name:Vrk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1261847. Vrk1.

Subcellular locationi

Cytoplasm. Nucleus. Cytoplasmcytoskeletonspindle By similarity
Note: Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-KW
  2. Golgi stack Source: UniProtKB
  3. nucleolus Source: Ensembl
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Serine/threonine-protein kinase VRK1PRO_0000086804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei342 – 3421Phosphoserine; by PLK3By similarity

Post-translational modificationi

Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis (By similarity). Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ80X41.
PaxDbiQ80X41.
PRIDEiQ80X41.

PTM databases

PhosphoSiteiQ80X41.

Expressioni

Tissue specificityi

Highly expressed in testis. Expressed in liver, kidney and muscle. Weakly expressed in thymus, bone marrow and spleen.2 Publications

Developmental stagei

Expressed from E10.5 to E14 in developing liver and then decreases. It increases again from E17.5 and remains thereafter. Highly expressed in hematopoietic embryonic tissues from E10.5 to E14.5. Weakly expressed in the yolk-sac.1 Publication

Gene expression databases

BgeeiQ80X41.
CleanExiMM_VRK1.
ExpressionAtlasiQ80X41. baseline and differential.
GenevestigatoriQ80X41.

Interactioni

Protein-protein interaction databases

BioGridi204536. 1 interaction.
IntActiQ80X41. 1 interaction.
MINTiMINT-4140113.

Structurei

3D structure databases

ProteinModelPortaliQ80X41.
SMRiQ80X41. Positions 1-388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 317281Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG330134.
GeneTreeiENSGT00760000119040.
HOGENOMiHOG000069991.
HOVERGENiHBG007532.
InParanoidiQ80X41.
KOiK08816.
OMAiYCMVQWL.
OrthoDBiEOG7KSX83.
PhylomeDBiQ80X41.
TreeFamiTF106473.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q80X41-1) [UniParc]FASTAAdd to Basket

Also known as: L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRVKAAQAG RPGPAKRRLA EQFAAGEVLT DMSRKEWKLG LPIGQGGFGC
60 70 80 90 100
IYLADTNSSK PVGSDAPCVV KVEPSDNGPL FTELKFYQRA AKPEQIQKWI
110 120 130 140 150
RTHKLKYLGV PKYWGSGLHD KNGKSYRFMI MDRFGSDLQK IYEANAKRFS
160 170 180 190 200
RKTVLQLSLR ILDILEYIHE HEYVHGDIKA SNLLLSHKNP DQVYLVDYGL
210 220 230 240 250
AYRYCPDGVH KEYKEDPKRC HDGTLEFTSI DAHKGVAPSR RGDLEILGYC
260 270 280 290 300
MIQWLSGCLP WEDNLKDPNY VRDSKIRYRD NVAALMEKCF PEKNKPGEIA
310 320 330 340 350
KYMESVKLLE YTEKPLYQNL RDILLQGLKA IGSKDDGKLD FSAVENGSVK
360 370 380 390 400
TRPASKKRKK EAEESAVCAV EDMECSDTQV QEAAQTRSVE SQGAIHGSMS
410 420 430 440
QPAAGCSSSD SSRRQQHLGL EQDMLRLDRR GSRTRKKAQK
Length:440
Mass (Da):49,741
Last modified:October 10, 2003 - v2
Checksum:i8318BA76A44B16BF
GO
Isoform 2 (identifier: Q80X41-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     388-431: Missing.

Note: No experimental confirmation available.

Show »
Length:396
Mass (Da):45,032
Checksum:i9F0FD5E3E685E545
GO
Isoform 3 (identifier: Q80X41-3) [UniParc]FASTAAdd to Basket

Also known as: S

The sequence of this isoform differs from the canonical sequence as follows:
     388-411: Missing.

Show »
Length:416
Mass (Da):47,478
Checksum:i6E15EE21E4180E26
GO
Isoform 5 (identifier: Q80X41-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     125-126: Missing.
     388-411: Missing.

Note: No experimental confirmation available.

Show »
Length:414
Mass (Da):47,228
Checksum:iA39A1A135715034B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei125 – 1262Missing in isoform 5. 1 PublicationVSP_008528
Alternative sequencei388 – 43144Missing in isoform 2. 1 PublicationVSP_008529Add
BLAST
Alternative sequencei388 – 41124Missing in isoform 3 and isoform 5. 2 PublicationsVSP_008532Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080252 mRNA. Translation: AAC29496.1.
AF080253 mRNA. Translation: AAC29497.1.
AK032344 mRNA. Translation: BAC27825.1.
AK088230 mRNA. Translation: BAC40225.1.
AK088674 mRNA. Translation: BAC40497.1.
BC016676 mRNA. Translation: AAH16676.1.
CCDSiCCDS26157.1. [Q80X41-3]
CCDS26158.1. [Q80X41-2]
CCDS36551.1. [Q80X41-1]
RefSeqiNP_001025014.1. NM_001029843.1. [Q80X41-3]
NP_001025015.1. NM_001029844.1. [Q80X41-2]
NP_035835.1. NM_011705.3. [Q80X41-1]
XP_006515872.1. XM_006515809.1. [Q80X41-1]
XP_006515873.1. XM_006515810.1. [Q80X41-1]
XP_006515874.1. XM_006515811.1. [Q80X41-1]
XP_006515875.1. XM_006515812.1. [Q80X41-1]
XP_006515881.1. XM_006515818.1. [Q80X41-1]
UniGeneiMm.2981.

Genome annotation databases

EnsembliENSMUST00000021539; ENSMUSP00000021539; ENSMUSG00000021115. [Q80X41-1]
ENSMUST00000072040; ENSMUSP00000071922; ENSMUSG00000021115. [Q80X41-2]
ENSMUST00000085026; ENSMUSP00000082101; ENSMUSG00000021115. [Q80X41-3]
GeneIDi22367.
KEGGimmu:22367.
UCSCiuc007ozb.1. mouse. [Q80X41-1]
uc007ozd.1. mouse. [Q80X41-2]
uc007oze.1. mouse. [Q80X41-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080252 mRNA. Translation: AAC29496.1 .
AF080253 mRNA. Translation: AAC29497.1 .
AK032344 mRNA. Translation: BAC27825.1 .
AK088230 mRNA. Translation: BAC40225.1 .
AK088674 mRNA. Translation: BAC40497.1 .
BC016676 mRNA. Translation: AAH16676.1 .
CCDSi CCDS26157.1. [Q80X41-3 ]
CCDS26158.1. [Q80X41-2 ]
CCDS36551.1. [Q80X41-1 ]
RefSeqi NP_001025014.1. NM_001029843.1. [Q80X41-3 ]
NP_001025015.1. NM_001029844.1. [Q80X41-2 ]
NP_035835.1. NM_011705.3. [Q80X41-1 ]
XP_006515872.1. XM_006515809.1. [Q80X41-1 ]
XP_006515873.1. XM_006515810.1. [Q80X41-1 ]
XP_006515874.1. XM_006515811.1. [Q80X41-1 ]
XP_006515875.1. XM_006515812.1. [Q80X41-1 ]
XP_006515881.1. XM_006515818.1. [Q80X41-1 ]
UniGenei Mm.2981.

3D structure databases

ProteinModelPortali Q80X41.
SMRi Q80X41. Positions 1-388.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204536. 1 interaction.
IntActi Q80X41. 1 interaction.
MINTi MINT-4140113.

PTM databases

PhosphoSitei Q80X41.

Proteomic databases

MaxQBi Q80X41.
PaxDbi Q80X41.
PRIDEi Q80X41.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021539 ; ENSMUSP00000021539 ; ENSMUSG00000021115 . [Q80X41-1 ]
ENSMUST00000072040 ; ENSMUSP00000071922 ; ENSMUSG00000021115 . [Q80X41-2 ]
ENSMUST00000085026 ; ENSMUSP00000082101 ; ENSMUSG00000021115 . [Q80X41-3 ]
GeneIDi 22367.
KEGGi mmu:22367.
UCSCi uc007ozb.1. mouse. [Q80X41-1 ]
uc007ozd.1. mouse. [Q80X41-2 ]
uc007oze.1. mouse. [Q80X41-5 ]

Organism-specific databases

CTDi 7443.
MGIi MGI:1261847. Vrk1.

Phylogenomic databases

eggNOGi NOG330134.
GeneTreei ENSGT00760000119040.
HOGENOMi HOG000069991.
HOVERGENi HBG007532.
InParanoidi Q80X41.
KOi K08816.
OMAi YCMVQWL.
OrthoDBi EOG7KSX83.
PhylomeDBi Q80X41.
TreeFami TF106473.

Enzyme and pathway databases

Reactomei REACT_210180. Initiation of Nuclear Envelope Reformation.
REACT_221677. Clearance of Nuclear Envelope Membranes from Chromatin.

Miscellaneous databases

NextBioi 302689.
PROi Q80X41.
SOURCEi Search...

Gene expression databases

Bgeei Q80X41.
CleanExi MM_VRK1.
ExpressionAtlasi Q80X41. baseline and differential.
Genevestigatori Q80X41.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel nuclear protein kinase in mice."
    Zelko I.N., Kobayashi R., Honkakoski P., Negishi M.
    Arch. Biochem. Biophys. 352:31-36(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), PROTEIN SEQUENCE OF 19-33; 267-275 AND 278-288, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AUTOPHOSPHORYLATION.
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 5).
    Strain: NOD.
    Tissue: Olfactory bulb and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Mammary tumor and Olfactory epithelium.
  4. "Expression of the VRK (vaccinia-related kinase) gene family of p53 regulators in murine hematopoietic development."
    Vega F.M., Gonzalo P., Gaspar M.L., Lazo P.A.
    FEBS Lett. 544:176-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "Characterization of three paralogous members of the Mammalian vaccinia related kinase family."
    Nichols R.J., Traktman P.
    J. Biol. Chem. 279:7934-7946(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.

Entry informationi

Entry nameiVRK1_MOUSE
AccessioniPrimary (citable) accession number: Q80X41
Secondary accession number(s): O88635
, O88636, Q8C2P7, Q91YH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 10, 2003
Last modified: November 26, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3