Q80X41 (VRK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase VRK1
EC=2.7.11.1

Alternative name(s):
Serine/threonine-protein kinase 51PK
Vaccinia-related kinase 1

Gene names

Name:

Vrk1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm By similarity. Ref.1 Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Active in presence of Mn2+, Mg2+ and Zn2+, but is not functional with Ca2+ or Cu2+. Has a higher affinity for Mn2+ than for Mg2+. RAN inhibits its autophosphorylation and its ability to phosphorylate histone H3 By similarity.
Subcellular location	Cytoplasm. Nucleus. Cytoplasm › cytoskeleton › spindle By similarity. Note: Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis. Ref.1 Ref.5
Tissue specificity	Highly expressed in testis. Expressed in liver, kidney and muscle. Weakly expressed in thymus, bone marrow and spleen. Ref.1 Ref.4
Developmental stage	Expressed from E10.5 to E14 in developing liver and then decreases. It increases again from E17.5 and remains thereafter. Highly expressed in hematopoietic embryonic tissues from E10.5 to E14.5. Weakly expressed in the yolk-sac. Ref.4
Post-translational modification	Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis By similarity. Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53. Ref.1 Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Golgi disassembly Inferred from sequence or structural similarity. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein autophosphorylation Inferred from direct assay Ref.5. Source: MGI
Cellular_component	Golgi stack Inferred from sequence or structural similarity. Source: UniProtKB nucleolus Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay Ref.5. Source: MGI spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from direct assay Ref.5. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q80X41-1)

Also known as: L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q80X41-2)

The sequence of this isoform differs from the canonical sequence as follows:
     388-431: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q80X41-3)

Also known as: S;

The sequence of this isoform differs from the canonical sequence as follows:
     388-411: Missing.

Isoform 5 (identifier: Q80X41-5)

The sequence of this isoform differs from the canonical sequence as follows:
     125-126: Missing.
     388-411: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Serine/threonine-protein kinase VRK1		PRO_0000086804
Regions
	Domain	37 – 317	281	Protein kinase
	Nucleotide binding	43 – 51	9	ATP By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	71	1	ATP By similarity
Amino acid modifications
	Modified residue	342	1	Phosphoserine; by PLK3 By similarity
Natural variations
	Alternative sequence	125 – 126	2	Missing in isoform 5.		VSP_008528
	Alternative sequence	388 – 431	44	Missing in isoform 2.		VSP_008529
	Alternative sequence	388 – 411	24	Missing in isoform 3 and isoform 5.		VSP_008532

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (L) [UniParc]. Last modified October 10, 2003. Version 2. Checksum: 8318BA76A44B16BF	FASTA	440	49,741
	10 20 30 40 50 60 MPRVKAAQAG RPGPAKRRLA EQFAAGEVLT DMSRKEWKLG LPIGQGGFGC IYLADTNSSK 70 80 90 100 110 120 PVGSDAPCVV KVEPSDNGPL FTELKFYQRA AKPEQIQKWI RTHKLKYLGV PKYWGSGLHD 130 140 150 160 170 180 KNGKSYRFMI MDRFGSDLQK IYEANAKRFS RKTVLQLSLR ILDILEYIHE HEYVHGDIKA 190 200 210 220 230 240 SNLLLSHKNP DQVYLVDYGL AYRYCPDGVH KEYKEDPKRC HDGTLEFTSI DAHKGVAPSR 250 260 270 280 290 300 RGDLEILGYC MIQWLSGCLP WEDNLKDPNY VRDSKIRYRD NVAALMEKCF PEKNKPGEIA 310 320 330 340 350 360 KYMESVKLLE YTEKPLYQNL RDILLQGLKA IGSKDDGKLD FSAVENGSVK TRPASKKRKK 370 380 390 400 410 420 EAEESAVCAV EDMECSDTQV QEAAQTRSVE SQGAIHGSMS QPAAGCSSSD SSRRQQHLGL 430 440 EQDMLRLDRR GSRTRKKAQK
	Isoform 2 [UniParc]. Checksum: 9F0FD5E3E685E545	FASTA	396	45,032
	10 20 30 40 50 60 MPRVKAAQAG RPGPAKRRLA EQFAAGEVLT DMSRKEWKLG LPIGQGGFGC IYLADTNSSK 70 80 90 100 110 120 PVGSDAPCVV KVEPSDNGPL FTELKFYQRA AKPEQIQKWI RTHKLKYLGV PKYWGSGLHD 130 140 150 160 170 180 KNGKSYRFMI MDRFGSDLQK IYEANAKRFS RKTVLQLSLR ILDILEYIHE HEYVHGDIKA 190 200 210 220 230 240 SNLLLSHKNP DQVYLVDYGL AYRYCPDGVH KEYKEDPKRC HDGTLEFTSI DAHKGVAPSR 250 260 270 280 290 300 RGDLEILGYC MIQWLSGCLP WEDNLKDPNY VRDSKIRYRD NVAALMEKCF PEKNKPGEIA 310 320 330 340 350 360 KYMESVKLLE YTEKPLYQNL RDILLQGLKA IGSKDDGKLD FSAVENGSVK TRPASKKRKK 370 380 390 EAEESAVCAV EDMECSDTQV QEAAQTRSRT RKKAQK
	Isoform 3 (S) [UniParc]. Checksum: 6E15EE21E4180E26	FASTA	416	47,478
	10 20 30 40 50 60 MPRVKAAQAG RPGPAKRRLA EQFAAGEVLT DMSRKEWKLG LPIGQGGFGC IYLADTNSSK 70 80 90 100 110 120 PVGSDAPCVV KVEPSDNGPL FTELKFYQRA AKPEQIQKWI RTHKLKYLGV PKYWGSGLHD 130 140 150 160 170 180 KNGKSYRFMI MDRFGSDLQK IYEANAKRFS RKTVLQLSLR ILDILEYIHE HEYVHGDIKA 190 200 210 220 230 240 SNLLLSHKNP DQVYLVDYGL AYRYCPDGVH KEYKEDPKRC HDGTLEFTSI DAHKGVAPSR 250 260 270 280 290 300 RGDLEILGYC MIQWLSGCLP WEDNLKDPNY VRDSKIRYRD NVAALMEKCF PEKNKPGEIA 310 320 330 340 350 360 KYMESVKLLE YTEKPLYQNL RDILLQGLKA IGSKDDGKLD FSAVENGSVK TRPASKKRKK 370 380 390 400 410 EAEESAVCAV EDMECSDTQV QEAAQTRSRR QQHLGLEQDM LRLDRRGSRT RKKAQK
	Isoform 5 [UniParc]. Checksum: A39A1A135715034B	FASTA	414	47,228
	10 20 30 40 50 60 MPRVKAAQAG RPGPAKRRLA EQFAAGEVLT DMSRKEWKLG LPIGQGGFGC IYLADTNSSK 70 80 90 100 110 120 PVGSDAPCVV KVEPSDNGPL FTELKFYQRA AKPEQIQKWI RTHKLKYLGV PKYWGSGLHD 130 140 150 160 170 180 KNGKRFMIMD RFGSDLQKIY EANAKRFSRK TVLQLSLRIL DILEYIHEHE YVHGDIKASN 190 200 210 220 230 240 LLLSHKNPDQ VYLVDYGLAY RYCPDGVHKE YKEDPKRCHD GTLEFTSIDA HKGVAPSRRG 250 260 270 280 290 300 DLEILGYCMI QWLSGCLPWE DNLKDPNYVR DSKIRYRDNV AALMEKCFPE KNKPGEIAKY 310 320 330 340 350 360 MESVKLLEYT EKPLYQNLRD ILLQGLKAIG SKDDGKLDFS AVENGSVKTR PASKKRKKEA 370 380 390 400 410 EESAVCAVED MECSDTQVQE AAQTRSRRQQ HLGLEQDMLR LDRRGSRTRK KAQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel nuclear protein kinase in mice." Zelko I.N., Kobayashi R., Honkakoski P., Negishi M. Arch. Biochem. Biophys. 352:31-36(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), PROTEIN SEQUENCE OF 19-33; 267-275 AND 278-288, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AUTOPHOSPHORYLATION. Tissue: Testis.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 5). Strain: NOD. Tissue: Olfactory bulb and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Mammary tumor and Olfactory epithelium.
[4]	"Expression of the VRK (vaccinia-related kinase) gene family of p53 regulators in murine hematopoietic development." Vega F.M., Gonzalo P., Gaspar M.L., Lazo P.A. FEBS Lett. 544:176-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]	"Characterization of three paralogous members of the Mammalian vaccinia related kinase family." Nichols R.J., Traktman P. J. Biol. Chem. 279:7934-7946(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF080252 mRNA. Translation: AAC29496.1. AF080253 mRNA. Translation: AAC29497.1. AK032344 mRNA. Translation: BAC27825.1. AK088230 mRNA. Translation: BAC40225.1. AK088674 mRNA. Translation: BAC40497.1. BC016676 mRNA. Translation: AAH16676.1.
IPI	IPI00132933. IPI00132936. IPI00377355. IPI00377358.
RefSeq	NP_001025014.1. NM_001029843.1. NP_001025015.1. NM_001029844.1. NP_035835.1. NM_011705.3.
UniGene	Mm.2981.
3D structure databases
ProteinModelPortal	Q80X41.
SMR	Q80X41. Positions 1-361.
ModBase	Search...
PTM databases
PhosphoSite	Q80X41.
Proteomic databases
PaxDb	Q80X41.
PRIDE	Q80X41.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000021539; ENSMUSP00000021539; ENSMUSG00000021115. ENSMUST00000072040; ENSMUSP00000071922; ENSMUSG00000021115. ENSMUST00000085026; ENSMUSP00000082101; ENSMUSG00000021115.
GeneID	22367.
KEGG	mmu:22367.
UCSC	uc007ozb.1. mouse. uc007ozd.1. mouse. uc007oze.1. mouse.
Organism-specific databases
CTD	7443.
MGI	MGI:1261847. Vrk1.
Phylogenomic databases
eggNOG	NOG330134.
GeneTree	ENSGT00690000101669.
HOGENOM	HOG000069991.
HOVERGEN	HBG007532.
InParanoid	Q80X41.
KO	K08816.
OMA	FMVMDRF.
OrthoDB	EOG4PG61C.
Gene expression databases
ArrayExpress	Q80X41.
Bgee	Q80X41.
CleanEx	MM_VRK1.
Genevestigator	Q80X41.
GermOnline	ENSMUSG00000021115. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302689.
SOURCE	Search...

Entry information

Entry name

VRK1_MOUSE

Accession

Primary (citable) accession number: Q80X41
Secondary accession number(s): O88635 , O88636, Q8C2P7, Q91YH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2003
Last sequence update:	October 10, 2003
Last modified:	April 3, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program