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Q80X41 (VRK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase VRK1

EC=2.7.11.1
Alternative name(s):
Serine/threonine-protein kinase 51PK
Vaccinia-related kinase 1
Gene names
Name:Vrk1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity By similarity. Ref.1 Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Active in presence of Mn2+, Mg2+ and Zn2+, but is not functional with Ca2+ or Cu2+. Has a higher affinity for Mn2+ than for Mg2+. RAN inhibits its autophosphorylation and its ability to phosphorylate histone H3 By similarity.

Subcellular location

Cytoplasm. Nucleus. Cytoplasmcytoskeletonspindle By similarity. Note: Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis. Ref.1 Ref.5

Tissue specificity

Highly expressed in testis. Expressed in liver, kidney and muscle. Weakly expressed in thymus, bone marrow and spleen. Ref.1 Ref.4

Developmental stage

Expressed from E10.5 to E14 in developing liver and then decreases. It increases again from E17.5 and remains thereafter. Highly expressed in hematopoietic embryonic tissues from E10.5 to E14.5. Weakly expressed in the yolk-sac. Ref.4

Post-translational modification

Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis By similarity. Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53. Ref.1 Ref.5

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.

Contains 1 protein kinase domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q80X41-1)

Also known as: L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q80X41-2)

The sequence of this isoform differs from the canonical sequence as follows:
     388-431: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q80X41-3)

Also known as: S;

The sequence of this isoform differs from the canonical sequence as follows:
     388-411: Missing.
Isoform 5 (identifier: Q80X41-5)

The sequence of this isoform differs from the canonical sequence as follows:
     125-126: Missing.
     388-411: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440Serine/threonine-protein kinase VRK1
PRO_0000086804

Regions

Domain37 – 317281Protein kinase
Nucleotide binding43 – 519ATP By similarity

Sites

Active site1771Proton acceptor By similarity
Binding site711ATP By similarity

Amino acid modifications

Modified residue3421Phosphoserine; by PLK3 By similarity

Natural variations

Alternative sequence125 – 1262Missing in isoform 5.
VSP_008528
Alternative sequence388 – 43144Missing in isoform 2.
VSP_008529
Alternative sequence388 – 41124Missing in isoform 3 and isoform 5.
VSP_008532

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (L) [UniParc].

Last modified October 10, 2003. Version 2.
Checksum: 8318BA76A44B16BF

FASTA44049,741
        10         20         30         40         50         60 
MPRVKAAQAG RPGPAKRRLA EQFAAGEVLT DMSRKEWKLG LPIGQGGFGC IYLADTNSSK 

        70         80         90        100        110        120 
PVGSDAPCVV KVEPSDNGPL FTELKFYQRA AKPEQIQKWI RTHKLKYLGV PKYWGSGLHD 

       130        140        150        160        170        180 
KNGKSYRFMI MDRFGSDLQK IYEANAKRFS RKTVLQLSLR ILDILEYIHE HEYVHGDIKA 

       190        200        210        220        230        240 
SNLLLSHKNP DQVYLVDYGL AYRYCPDGVH KEYKEDPKRC HDGTLEFTSI DAHKGVAPSR 

       250        260        270        280        290        300 
RGDLEILGYC MIQWLSGCLP WEDNLKDPNY VRDSKIRYRD NVAALMEKCF PEKNKPGEIA 

       310        320        330        340        350        360 
KYMESVKLLE YTEKPLYQNL RDILLQGLKA IGSKDDGKLD FSAVENGSVK TRPASKKRKK 

       370        380        390        400        410        420 
EAEESAVCAV EDMECSDTQV QEAAQTRSVE SQGAIHGSMS QPAAGCSSSD SSRRQQHLGL 

       430        440 
EQDMLRLDRR GSRTRKKAQK 

« Hide

Isoform 2 [UniParc].

Checksum: 9F0FD5E3E685E545
Show »

FASTA39645,032
Isoform 3 (S) [UniParc].

Checksum: 6E15EE21E4180E26
Show »

FASTA41647,478
Isoform 5 [UniParc].

Checksum: A39A1A135715034B
Show »

FASTA41447,228

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a novel nuclear protein kinase in mice."
Zelko I.N., Kobayashi R., Honkakoski P., Negishi M.
Arch. Biochem. Biophys. 352:31-36(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), PROTEIN SEQUENCE OF 19-33; 267-275 AND 278-288, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AUTOPHOSPHORYLATION.
Tissue: Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 5).
Strain: NOD.
Tissue: Olfactory bulb and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Mammary tumor and Olfactory epithelium.
[4]"Expression of the VRK (vaccinia-related kinase) gene family of p53 regulators in murine hematopoietic development."
Vega F.M., Gonzalo P., Gaspar M.L., Lazo P.A.
FEBS Lett. 544:176-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]"Characterization of three paralogous members of the Mammalian vaccinia related kinase family."
Nichols R.J., Traktman P.
J. Biol. Chem. 279:7934-7946(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF080252 mRNA. Translation: AAC29496.1.
AF080253 mRNA. Translation: AAC29497.1.
AK032344 mRNA. Translation: BAC27825.1.
AK088230 mRNA. Translation: BAC40225.1.
AK088674 mRNA. Translation: BAC40497.1.
BC016676 mRNA. Translation: AAH16676.1.
RefSeqNP_001025014.1. NM_001029843.1.
NP_001025015.1. NM_001029844.1.
NP_035835.1. NM_011705.3.
XP_006515872.1. XM_006515809.1.
XP_006515873.1. XM_006515810.1.
XP_006515874.1. XM_006515811.1.
XP_006515875.1. XM_006515812.1.
XP_006515881.1. XM_006515818.1.
UniGeneMm.2981.

3D structure databases

ProteinModelPortalQ80X41.
SMRQ80X41. Positions 1-361.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204536. 1 interaction.
IntActQ80X41. 1 interaction.
MINTMINT-4140113.

PTM databases

PhosphoSiteQ80X41.

Proteomic databases

PaxDbQ80X41.
PRIDEQ80X41.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021539; ENSMUSP00000021539; ENSMUSG00000021115. [Q80X41-1]
ENSMUST00000072040; ENSMUSP00000071922; ENSMUSG00000021115. [Q80X41-2]
ENSMUST00000085026; ENSMUSP00000082101; ENSMUSG00000021115. [Q80X41-3]
GeneID22367.
KEGGmmu:22367.
UCSCuc007ozb.1. mouse. [Q80X41-1]
uc007ozd.1. mouse. [Q80X41-2]
uc007oze.1. mouse. [Q80X41-5]

Organism-specific databases

CTD7443.
MGIMGI:1261847. Vrk1.

Phylogenomic databases

eggNOGNOG330134.
GeneTreeENSGT00730000110259.
HOGENOMHOG000069991.
HOVERGENHBG007532.
InParanoidQ80X41.
KOK08816.
OMAYCMVQWL.
OrthoDBEOG7KSX83.
PhylomeDBQ80X41.
TreeFamTF106473.

Gene expression databases

ArrayExpressQ80X41.
BgeeQ80X41.
CleanExMM_VRK1.
GenevestigatorQ80X41.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302689.
PROQ80X41.
SOURCESearch...

Entry information

Entry nameVRK1_MOUSE
AccessionPrimary (citable) accession number: Q80X41
Secondary accession number(s): O88635 expand/collapse secondary AC list , O88636, Q8C2P7, Q91YH9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 10, 2003
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot