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Protein

DDRGK domain-containing protein 1

Gene

Ddrgk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein which interacts with the E3 UFM1-protein ligase UFL1 and one of its substrates TRIP4 and is required for TRIP4 ufmylation. Through TRIP4 ufmylation may regulate nuclear receptors-mediated transcription. May play a role in NF-kappa-B-mediated transcription through regulation of the phosphorylation and the degradation of NFKBIB, the inhibitor of NF-kappa-B (By similarity). May also play a role in the cellular response to endoplasmic reticulum stress (PubMed:21494687).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
DDRGK domain-containing protein 1Curated
Alternative name(s):
UFM1-binding and PCI domain-containing protein 11 Publication
Gene namesi
Name:Ddrgk1Imported
Synonyms:Ufbp11 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1924256. Ddrgk1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Chaini29 – 315287DDRGK domain-containing protein 1PRO_0000021034Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki268 – 268Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1)By similarity

Post-translational modificationi

Ufmylated. Conjugated to ubiquitin-like protein UFM1, probably at Lys-268.1 Publication
Ubiquitinated. Ubiquitination probably triggers proteasomal degradation and is negatively regulated by UFL1, the enzyme involved in the ufmylation of DDRGK1.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ80WW9.
MaxQBiQ80WW9.
PaxDbiQ80WW9.
PRIDEiQ80WW9.

PTM databases

iPTMnetiQ80WW9.
PhosphoSiteiQ80WW9.

Expressioni

Tissue specificityi

Ubiquitously expressed (PubMed:20228063). Higher expression in pancreatic islets, pancreatic acini and testis (at protein level) (PubMed:21494687).2 Publications

Gene expression databases

BgeeiQ80WW9.
ExpressionAtlasiQ80WW9. baseline and differential.
GenevisibleiQ80WW9. MM.

Interactioni

Subunit structurei

Interacts with TRIP4; the interaction with TRIP4 is direct. Interacts with UFL1. Interacts with NFKBIB. Interacts with CDK5RAP3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi218455. 1 interaction.
STRINGi10090.ENSMUSP00000086988.

Structurei

Secondary structure

1
315
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi216 – 22611Combined sources
Beta strandi228 – 2303Combined sources
Helixi232 – 2398Combined sources
Helixi243 – 25614Combined sources
Beta strandi257 – 2593Combined sources
Beta strandi261 – 2633Combined sources
Beta strandi269 – 2713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WI9NMR-A216-274[»]
ProteinModelPortaliQ80WW9.
SMRiQ80WW9. Positions 213-274.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ80WW9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini230 – 27445PCIAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 115115Mediates interaction with CDK5RAP3By similarityAdd
BLAST
Regioni119 – 21799Mediates interaction with TRIP4By similarityAdd
BLAST
Regioni217 – 31599Mediates interaction with UFL1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the DDRGK1 family.Curated
Contains 1 PCI domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3054. Eukaryota.
ENOG4111IPW. LUCA.
GeneTreeiENSGT00390000017193.
HOGENOMiHOG000005758.
HOVERGENiHBG051191.
InParanoidiQ80WW9.
OMAiEERGAGM.
OrthoDBiEOG7SV0WW.
PhylomeDBiQ80WW9.
TreeFamiTF314645.

Family and domain databases

InterProiIPR019153. DDRGK_dom-contain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF09756. DDRGK. 1 hit.
[Graphical view]
SMARTiSM01128. DDRGK. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80WW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGPWVYLVA AVLLIGLILF LTRSRGRAAA ADGEPLHNEE ERAGAGQVGR
60 70 80 90 100
SLPQESEEQR TGSRPRRRRD LGSRLQAQRR AQRVAWEDGD ENVGQTVIPA
110 120 130 140 150
QEEEGIEKPA EVHPTGKIGA KKLRKLEEKQ ARKAQREAEE AEREERKRLE
160 170 180 190 200
SQREAEWKKE EERLRLKEEQ KEEEERKAQE EQARREHEEY LKLKEAFVVE
210 220 230 240 250
EEGVSETMTE EQSHSFLTEF INYIKKSKVV LLEDLAFQMG LRTQDAINRI
260 270 280 290 300
QDLLTEGTLT GVIDDRGKFI YITPEELAAV ANFIRQRGRV SITELAQASN
310
SLISWGQDLP AQASA
Length:315
Mass (Da):35,977
Last modified:September 26, 2003 - v2
Checksum:iD973B9AFBE265562
GO

Sequence cautioni

The sequence AAH51541.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL731707, AL772162 Genomic DNA. Translation: CAM13264.1.
AL772162, AL731707 Genomic DNA. Translation: CAM16448.1.
CH466519 Genomic DNA. Translation: EDL28284.1.
BC115616 mRNA. Translation: AAI15617.1.
BC115617 mRNA. Translation: AAI15618.1.
BC051541 mRNA. Translation: AAH51541.1. Different initiation.
AK011170 mRNA. Translation: BAB27444.1.
CCDSiCCDS38243.1.
RefSeqiNP_084108.1. NM_029832.2.
UniGeneiMm.440063.

Genome annotation databases

EnsembliENSMUST00000089559; ENSMUSP00000086988; ENSMUSG00000068290.
GeneIDi77006.
KEGGimmu:77006.
UCSCiuc008mjs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL731707, AL772162 Genomic DNA. Translation: CAM13264.1.
AL772162, AL731707 Genomic DNA. Translation: CAM16448.1.
CH466519 Genomic DNA. Translation: EDL28284.1.
BC115616 mRNA. Translation: AAI15617.1.
BC115617 mRNA. Translation: AAI15618.1.
BC051541 mRNA. Translation: AAH51541.1. Different initiation.
AK011170 mRNA. Translation: BAB27444.1.
CCDSiCCDS38243.1.
RefSeqiNP_084108.1. NM_029832.2.
UniGeneiMm.440063.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WI9NMR-A216-274[»]
ProteinModelPortaliQ80WW9.
SMRiQ80WW9. Positions 213-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218455. 1 interaction.
STRINGi10090.ENSMUSP00000086988.

PTM databases

iPTMnetiQ80WW9.
PhosphoSiteiQ80WW9.

Proteomic databases

EPDiQ80WW9.
MaxQBiQ80WW9.
PaxDbiQ80WW9.
PRIDEiQ80WW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089559; ENSMUSP00000086988; ENSMUSG00000068290.
GeneIDi77006.
KEGGimmu:77006.
UCSCiuc008mjs.1. mouse.

Organism-specific databases

CTDi65992.
MGIiMGI:1924256. Ddrgk1.

Phylogenomic databases

eggNOGiKOG3054. Eukaryota.
ENOG4111IPW. LUCA.
GeneTreeiENSGT00390000017193.
HOGENOMiHOG000005758.
HOVERGENiHBG051191.
InParanoidiQ80WW9.
OMAiEERGAGM.
OrthoDBiEOG7SV0WW.
PhylomeDBiQ80WW9.
TreeFamiTF314645.

Miscellaneous databases

EvolutionaryTraceiQ80WW9.
PROiQ80WW9.
SOURCEiSearch...

Gene expression databases

BgeeiQ80WW9.
ExpressionAtlasiQ80WW9. baseline and differential.
GenevisibleiQ80WW9. MM.

Family and domain databases

InterProiIPR019153. DDRGK_dom-contain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF09756. DDRGK. 1 hit.
[Graphical view]
SMARTiSM01128. DDRGK. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Lung.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-171.
    Strain: C57BL/6J.
    Tissue: Embryo.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  6. "A novel C53/LZAP-interacting protein regulates stability of C53/LZAP and DDRGK domain-containing Protein 1 (DDRGK1) and modulates NF-kappaB signaling."
    Wu J., Lei G., Mei M., Tang Y., Li H.
    J. Biol. Chem. 285:15126-15136(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis."
    Lemaire K., Moura R.F., Granvik M., Igoillo-Esteve M., Hohmeier H.E., Hendrickx N., Newgard C.B., Waelkens E., Cnop M., Schuit F.
    PLoS ONE 6:E18517-E18517(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, UFMYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "Solution structure of the PCI domain from mouse hypothetical protein AAH51541."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 216-274.

Entry informationi

Entry nameiDDRGK_MOUSE
AccessioniPrimary (citable) accession number: Q80WW9
Secondary accession number(s): Q14BT4, Q9CT52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: June 8, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.