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Q80WV3

- CHST2_MOUSE

UniProt

Q80WV3 - CHST2_MOUSE

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Protein

Carbohydrate sulfotransferase 2

Gene

Chst2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi173 – 1797PAPSBy similarity
Nucleotide bindingi332 – 3409PAPSBy similarity

GO - Molecular functioni

  1. N-acetylglucosamine 6-O-sulfotransferase activity Source: Ensembl
  2. sulfotransferase activity Source: MGI

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. inflammatory response Source: UniProtKB-KW
  3. N-acetylglucosamine metabolic process Source: MGI
  4. sulfur compound metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism, Inflammatory response

Enzyme and pathway databases

ReactomeiREACT_198578. Keratan sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 2 (EC:2.8.2.-)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2
Short name:
GST-2
N-acetylglucosamine 6-O-sulfotransferase 1
Short name:
GlcNAc6ST-1
Short name:
Gn6st-1
Gene namesi
Name:Chst2
Synonyms:Gst2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1891160. Chst2.

Subcellular locationi

GO - Cellular componenti

  1. Golgi membrane Source: InterPro
  2. integral component of membrane Source: UniProtKB-KW
  3. trans-Golgi network Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are impaired in the elaboration of sialyl 6-sulfo Lewis X in HEV. Lymphocyte homing to peripheral lymph nodes, mesenteric lymph nodes, and Peyer patches are significantly reduced.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Carbohydrate sulfotransferase 2PRO_0000085187Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi243 – 2431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi457 – 4571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi475 – 4751N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ80WV3.
PaxDbiQ80WV3.
PRIDEiQ80WV3.

PTM databases

PhosphoSiteiQ80WV3.

Expressioni

Tissue specificityi

In brain, it is expressed in pyramidal cells in the CA3 subregion of the hippocampus, cerebellar nucleus and Purkinje cells.2 Publications

Gene expression databases

BgeeiQ80WV3.
CleanExiMM_CHST2.
ExpressionAtlasiQ80WV3. baseline and differential.
GenevestigatoriQ80WV3.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Homodimerization is not essential for enzyme activity (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ80WV3.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5454CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini76 – 530455LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei55 – 7521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG67451.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ80WV3.
KOiK04745.
OMAiMGGPADY.
OrthoDBiEOG7RZ5S0.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequencei

Sequence statusi: Complete.

Q80WV3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRSSPRALP PGALPRPLPA APAAVQRALL PPWPRRAGRR WPASPLGMKV
60 70 80 90 100
FRRKALVLCA GYALLLVLTM LNLLDYKWHK EPLQQCNPDG PLGAAVGAAG
110 120 130 140 150
AGWGRPGSPP AAPPRAHSRM DPRTPYRPPA AGVGAVPAAA AGSAGAAASL
160 170 180 190 200
GNATRGTRGG GDKRQLVYVF TTWRSGSSFF GELFNQNPEV FFLYEPVWHV
210 220 230 240 250
WQKLYPGDAV SLQGAARDML SALYRCDLSV FQLYSPAGSG GRNLTTLGIF
260 270 280 290 300
GAATNKVVCS SPLCPAYRKE VVGLVDDRVC KKCPPQRLAR FEEECRKYRT
310 320 330 340 350
LVIKGVRVFD VAVLAPLLKD PALDLKVIHL VRDPRAVASS RIRSRHGLIR
360 370 380 390 400
ESLQVVRSRD PRAHRMPFLE AAGHKLGAKK EGMGGPADYH ALGAMEVICN
410 420 430 440 450
SMAKTLQTAL QPPDWLQGHY LVVRYEDLVG DPVKTLRRVY DFVGLLVSPE
460 470 480 490 500
MEQFALNMTS GSGSSSKPFV VSARNATQAA NAWRTALTFQ QIKQVEEFCY
510 520 530
QPMAVLGYER VNSPEEVKDL SKTLLRKPRL
Length:530
Mass (Da):57,828
Last modified:July 27, 2011 - v3
Checksum:i275363BF15440730
GO

Sequence cautioni

The sequence AAH51963.2 differs from that shown. Reason: Erroneous initiation.
The sequence BAA32137.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAA32139.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAD16775.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti301 – 3011L → V in BAA32137. (PubMed:9712885)Curated
Sequence conflicti301 – 3011L → V in BAA32139. (PubMed:9712885)Curated
Sequence conflicti301 – 3011L → V in BAA32138. (PubMed:9712885)Curated
Sequence conflicti301 – 3011L → V in BAD16775. (PubMed:15175329)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011451 mRNA. Translation: BAA32137.1. Different initiation.
AB011452 mRNA. Translation: BAA32138.1.
AB011452 mRNA. Translation: BAA32139.1. Different initiation.
AB125058 Genomic DNA. Translation: BAD16775.1. Different initiation.
AC144813 Genomic DNA. No translation available.
BC051963 mRNA. Translation: AAH51963.2. Different initiation.
CCDSiCCDS52888.1.
RefSeqiNP_061233.2. NM_018763.2.
UniGeneiMm.212446.

Genome annotation databases

EnsembliENSMUST00000036267; ENSMUSP00000040775; ENSMUSG00000033350.
GeneIDi54371.
KEGGimmu:54371.
UCSCiuc009raz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011451 mRNA. Translation: BAA32137.1 . Different initiation.
AB011452 mRNA. Translation: BAA32138.1 .
AB011452 mRNA. Translation: BAA32139.1 . Different initiation.
AB125058 Genomic DNA. Translation: BAD16775.1 . Different initiation.
AC144813 Genomic DNA. No translation available.
BC051963 mRNA. Translation: AAH51963.2 . Different initiation.
CCDSi CCDS52888.1.
RefSeqi NP_061233.2. NM_018763.2.
UniGenei Mm.212446.

3D structure databases

ProteinModelPortali Q80WV3.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q80WV3.

Proteomic databases

MaxQBi Q80WV3.
PaxDbi Q80WV3.
PRIDEi Q80WV3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000036267 ; ENSMUSP00000040775 ; ENSMUSG00000033350 .
GeneIDi 54371.
KEGGi mmu:54371.
UCSCi uc009raz.2. mouse.

Organism-specific databases

CTDi 9435.
MGIi MGI:1891160. Chst2.

Phylogenomic databases

eggNOGi NOG67451.
GeneTreei ENSGT00530000062902.
HOGENOMi HOG000261614.
HOVERGENi HBG050949.
InParanoidi Q80WV3.
KOi K04745.
OMAi MGGPADY.
OrthoDBi EOG7RZ5S0.
TreeFami TF342871.

Enzyme and pathway databases

Reactomei REACT_198578. Keratan sulfate biosynthesis.

Miscellaneous databases

ChiTaRSi CHST2. mouse.
NextBioi 311174.
PROi Q80WV3.
SOURCEi Search...

Gene expression databases

Bgeei Q80WV3.
CleanExi MM_CHST2.
ExpressionAtlasi Q80WV3. baseline and differential.
Genevestigatori Q80WV3.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view ]
Pfami PF00685. Sulfotransfer_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMi SSF52540. SSF52540. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of an N-acetylglucosamine-6-O-sulfotransferase."
    Uchimura K., Muramatsu H., Kadomatsu K., Fan Q.-W., Kurosawa N., Mitsuoka C., Kannagi R., Habuchi O., Muramatsu T.
    J. Biol. Chem. 273:22577-22583(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Embryo.
  2. "N-acetylglucosamine 6-O-sulfotransferase-1 regulates expression of L-selectin ligands and lymphocyte homing."
    Uchimura K., Kadomatsu K., El-Fasakhany F.M., Singer M.S., Izawa M., Kannagi R., Takeda N., Rosen S.D., Muramatsu T.
    J. Biol. Chem. 279:35001-35008(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE, FUNCTION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Human N-acetylglucosamine-6-O-sulfotransferase involved in the biosynthesis of 6-sulfo sialyl Lewis X: molecular cloning, chromosomal mapping, and expression in various organs and tumor cells."
    Uchimura K., Muramatsu H., Kaname T., Ogawa H., Yamakawa T., Fan Q.-W., Mitsuoka C., Kannagi R., Habuchi O., Yokoyama I., Yamamura K., Ozaki T., Nakagawara A., Kadomatsu K., Muramatsu T.
    J. Biochem. 124:670-678(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCHST2_MOUSE
AccessioniPrimary (citable) accession number: Q80WV3
Secondary accession number(s): E9QNG2, O88276, Q794G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-48 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3