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Protein

Aftiphilin

Gene

Aftph

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in membrane trafficking.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Aftiphilin
Gene namesi
Name:Aftph
Synonyms:Afth
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1923012. Aftph.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Colocalizes with AP1G1 and clathrin.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000644891 – 931AftiphilinAdd BLAST931

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei151PhosphoserineBy similarity1
Modified residuei395PhosphoserineCombined sources1
Modified residuei613PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80WT5.
PeptideAtlasiQ80WT5.
PRIDEiQ80WT5.

PTM databases

iPTMnetiQ80WT5.
PhosphoSitePlusiQ80WT5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000049659.
CleanExiMM_AFTPH.
ExpressionAtlasiQ80WT5. baseline and differential.
GenevisibleiQ80WT5. MM.

Interactioni

Subunit structurei

Interacts with GGA1, GGA3, AP1G1 and AP1G2 via their GAE domain.By similarity

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-1344435.
STRINGi10090.ENSMUSP00000036778.

Structurei

3D structure databases

ProteinModelPortaliQ80WT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni821 – 825Clathrin-bindingSequence analysis5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi28 – 31WXXF motif 14
Motifi433 – 436WXXF motif 24
Motifi476 – 479WXXF motif 34

Domaini

The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself (By similarity).By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IIS8. Eukaryota.
ENOG4111EYY. LUCA.
GeneTreeiENSGT00390000017835.
HOGENOMiHOG000033840.
HOVERGENiHBG050464.
InParanoidiQ80WT5.
OMAiETVNPQG.
OrthoDBiEOG091G07VV.
PhylomeDBiQ80WT5.
TreeFamiTF331532.

Family and domain databases

InterProiIPR029205. Clathrin-bd.
[Graphical view]
PfamiPF15045. Clathrin_bdg. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80WT5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPDIIRMYS SSPPPLDNGA EDDEEDEFGE FGGFSEVSPS GVGFVDFDTP
60 70 80 90 100
DYTRPKEDFV PSNHFMPIHE YSEDVDSLTS FKSVQNGNDK DITAELSTPV
110 120 130 140 150
KSQSDVVLST TSKEMIPSKT LDPSIDGMES LEDLDKVVVQ GPSTGQLRSF
160 170 180 190 200
SPGDFRTDKN IVHQTKQLES CNGEKPPCLE ILTNGFAGLE TVNPQGTDDL
210 220 230 240 250
DNVADSKGSK PLNTCGTECI LESAASHATE FADFSTFSQT ERTQLEEIEC
260 270 280 290 300
PVLNDGDTLT IQGNSKGPRV KELNCVKEVT LDGSFEDTGN TEREHQVCVS
310 320 330 340 350
EIHAVADRGL SVEKQDLQTL QQDEFLNSRI QSEAWSLVDS SENSEAITKE
360 370 380 390 400
RCKMEKNDLF ASKCADLSMD SVKTSDVNEI GSSKEENRKL TNPKSPDPDP
410 420 430 440 450
TGQNALDDSA ASMKNGDSGN GFVTCHDTNE DDFGDFGTAN GTTPPFVTST
460 470 480 490 500
QDSMSDVTFE DSSEHFLHLS EPGDDFGEFE DTNAVSCQEE MRFTESDLRQ
510 520 530 540 550
TSDGLSEECP LAGESGGKDS KPDSKLKNGQ DSEFGDFDSV PNTQGSAFQD
560 570 580 590 600
SDDFADFSSA GPSQAVDWNA FEDEQKDGCS WAAFGDQQET ESHHLKEVWQ
610 620 630 640 650
SQRTDETMGT LGTPKMHSVS SAASKGAVAS GHLQEPGTSV QTALLNRLER
660 670 680 690 700
IFEACFPSVF VPDVEEEVSS LKHLLETHSS PAKTREALAD RGELRGVWTE
710 720 730 740 750
LQDIHDAHGL RYQWGGSHSN KKLLCSLGID TRNILFTGNK KQPVIVPMYA
760 770 780 790 800
AGLGMLEPTK EPLKPLSAAE KIASIGQTTV MTPEINTCTS DPFQESLPPV
810 820 830 840 850
QFDWSSSGLT NPLDASGGST LLNLDFFGPV DDSSSSSSTI PGVDPELYEL
860 870 880 890 900
TTAKLETSTS SLRVTDAFAK LMSTVEKTST STRKPKREEH LSEEAMKVIA
910 920 930
SLPDLTFMHA KVLMFPATLT PSMSSQEQAD A
Length:931
Mass (Da):101,131
Last modified:March 29, 2005 - v2
Checksum:iFE1C64F3B7270620
GO
Isoform 2 (identifier: Q80WT5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     815-841: Missing.

Show »
Length:904
Mass (Da):98,490
Checksum:i506C3468B7BD598E
GO

Sequence cautioni

The sequence CAI24910 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI25738 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013242815 – 841Missing in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL663115, AL645599 Genomic DNA. Translation: CAI25737.1.
AL663115, AL645599 Genomic DNA. Translation: CAI25738.1. Sequence problems.
AL645599, AL663115 Genomic DNA. Translation: CAI24909.1.
AL645599, AL663115 Genomic DNA. Translation: CAI24910.1. Sequence problems.
BC004630 mRNA. Translation: AAH04630.1.
BC052036 mRNA. Translation: AAH52036.1.
CCDSiCCDS24459.1. [Q80WT5-2]
CCDS70146.1. [Q80WT5-1]
RefSeqiNP_001239432.1. NM_001252503.2.
NP_001277474.1. NM_001290545.1. [Q80WT5-1]
NP_852076.1. NM_181411.4. [Q80WT5-2]
UniGeneiMm.356580.
Mm.489893.

Genome annotation databases

EnsembliENSMUST00000035350; ENSMUSP00000036778; ENSMUSG00000049659. [Q80WT5-2]
ENSMUST00000146722; ENSMUSP00000121612; ENSMUSG00000049659. [Q80WT5-1]
GeneIDi216549.
KEGGimmu:216549.
UCSCiuc007idh.3. mouse. [Q80WT5-2]
uc056yjx.1. mouse. [Q80WT5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL663115, AL645599 Genomic DNA. Translation: CAI25737.1.
AL663115, AL645599 Genomic DNA. Translation: CAI25738.1. Sequence problems.
AL645599, AL663115 Genomic DNA. Translation: CAI24909.1.
AL645599, AL663115 Genomic DNA. Translation: CAI24910.1. Sequence problems.
BC004630 mRNA. Translation: AAH04630.1.
BC052036 mRNA. Translation: AAH52036.1.
CCDSiCCDS24459.1. [Q80WT5-2]
CCDS70146.1. [Q80WT5-1]
RefSeqiNP_001239432.1. NM_001252503.2.
NP_001277474.1. NM_001290545.1. [Q80WT5-1]
NP_852076.1. NM_181411.4. [Q80WT5-2]
UniGeneiMm.356580.
Mm.489893.

3D structure databases

ProteinModelPortaliQ80WT5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1344435.
STRINGi10090.ENSMUSP00000036778.

PTM databases

iPTMnetiQ80WT5.
PhosphoSitePlusiQ80WT5.

Proteomic databases

PaxDbiQ80WT5.
PeptideAtlasiQ80WT5.
PRIDEiQ80WT5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035350; ENSMUSP00000036778; ENSMUSG00000049659. [Q80WT5-2]
ENSMUST00000146722; ENSMUSP00000121612; ENSMUSG00000049659. [Q80WT5-1]
GeneIDi216549.
KEGGimmu:216549.
UCSCiuc007idh.3. mouse. [Q80WT5-2]
uc056yjx.1. mouse. [Q80WT5-1]

Organism-specific databases

CTDi54812.
MGIiMGI:1923012. Aftph.

Phylogenomic databases

eggNOGiENOG410IIS8. Eukaryota.
ENOG4111EYY. LUCA.
GeneTreeiENSGT00390000017835.
HOGENOMiHOG000033840.
HOVERGENiHBG050464.
InParanoidiQ80WT5.
OMAiETVNPQG.
OrthoDBiEOG091G07VV.
PhylomeDBiQ80WT5.
TreeFamiTF331532.

Miscellaneous databases

ChiTaRSiAftph. mouse.
PROiQ80WT5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049659.
CleanExiMM_AFTPH.
ExpressionAtlasiQ80WT5. baseline and differential.
GenevisibleiQ80WT5. MM.

Family and domain databases

InterProiIPR029205. Clathrin-bd.
[Graphical view]
PfamiPF15045. Clathrin_bdg. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAFTIN_MOUSE
AccessioniPrimary (citable) accession number: Q80WT5
Secondary accession number(s): Q5SSE6, Q99KJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.