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Q80WT5

- AFTIN_MOUSE

UniProt

Q80WT5 - AFTIN_MOUSE

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Protein

Aftiphilin

Gene

Aftph

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

May play a role in membrane trafficking.By similarity

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Aftiphilin
Gene namesi
Name:Aftph
Synonyms:Afth
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1923012. Aftph.

Subcellular locationi

Cytoplasm By similarity
Note: Colocalizes with AP1G1 and clathrin.By similarity

GO - Cellular componenti

  1. AP-1 adaptor complex Source: Ensembl
  2. cytosol Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931AftiphilinPRO_0000064489Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511PhosphoserineBy similarity
Modified residuei395 – 3951Phosphoserine2 Publications
Modified residuei613 – 6131PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ80WT5.
PaxDbiQ80WT5.
PRIDEiQ80WT5.

PTM databases

PhosphoSiteiQ80WT5.

Expressioni

Gene expression databases

BgeeiQ80WT5.
CleanExiMM_AFTPH.
ExpressionAtlasiQ80WT5. baseline and differential.
GenevestigatoriQ80WT5.

Interactioni

Subunit structurei

Interacts with GGA1, GGA3, AP1G1 and AP1G2 via their GAE domain.By similarity

Protein-protein interaction databases

MINTiMINT-1344435.
STRINGi10090.ENSMUSP00000091938.

Structurei

3D structure databases

ProteinModelPortaliQ80WT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni821 – 8255Clathrin-bindingSequence Analysis

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi28 – 314WXXF motif 1
Motifi433 – 4364WXXF motif 2
Motifi476 – 4794WXXF motif 3

Domaini

The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself (By similarity).By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG40359.
GeneTreeiENSGT00390000017835.
HOGENOMiHOG000033840.
HOVERGENiHBG050464.
InParanoidiQ80WT5.
OMAiETVNPQG.
OrthoDBiEOG779NX4.
PhylomeDBiQ80WT5.
TreeFamiTF331532.

Family and domain databases

InterProiIPR029205. Clathrin-bd.
[Graphical view]
PfamiPF15045. Clathrin_bdg. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q80WT5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPDIIRMYS SSPPPLDNGA EDDEEDEFGE FGGFSEVSPS GVGFVDFDTP
60 70 80 90 100
DYTRPKEDFV PSNHFMPIHE YSEDVDSLTS FKSVQNGNDK DITAELSTPV
110 120 130 140 150
KSQSDVVLST TSKEMIPSKT LDPSIDGMES LEDLDKVVVQ GPSTGQLRSF
160 170 180 190 200
SPGDFRTDKN IVHQTKQLES CNGEKPPCLE ILTNGFAGLE TVNPQGTDDL
210 220 230 240 250
DNVADSKGSK PLNTCGTECI LESAASHATE FADFSTFSQT ERTQLEEIEC
260 270 280 290 300
PVLNDGDTLT IQGNSKGPRV KELNCVKEVT LDGSFEDTGN TEREHQVCVS
310 320 330 340 350
EIHAVADRGL SVEKQDLQTL QQDEFLNSRI QSEAWSLVDS SENSEAITKE
360 370 380 390 400
RCKMEKNDLF ASKCADLSMD SVKTSDVNEI GSSKEENRKL TNPKSPDPDP
410 420 430 440 450
TGQNALDDSA ASMKNGDSGN GFVTCHDTNE DDFGDFGTAN GTTPPFVTST
460 470 480 490 500
QDSMSDVTFE DSSEHFLHLS EPGDDFGEFE DTNAVSCQEE MRFTESDLRQ
510 520 530 540 550
TSDGLSEECP LAGESGGKDS KPDSKLKNGQ DSEFGDFDSV PNTQGSAFQD
560 570 580 590 600
SDDFADFSSA GPSQAVDWNA FEDEQKDGCS WAAFGDQQET ESHHLKEVWQ
610 620 630 640 650
SQRTDETMGT LGTPKMHSVS SAASKGAVAS GHLQEPGTSV QTALLNRLER
660 670 680 690 700
IFEACFPSVF VPDVEEEVSS LKHLLETHSS PAKTREALAD RGELRGVWTE
710 720 730 740 750
LQDIHDAHGL RYQWGGSHSN KKLLCSLGID TRNILFTGNK KQPVIVPMYA
760 770 780 790 800
AGLGMLEPTK EPLKPLSAAE KIASIGQTTV MTPEINTCTS DPFQESLPPV
810 820 830 840 850
QFDWSSSGLT NPLDASGGST LLNLDFFGPV DDSSSSSSTI PGVDPELYEL
860 870 880 890 900
TTAKLETSTS SLRVTDAFAK LMSTVEKTST STRKPKREEH LSEEAMKVIA
910 920 930
SLPDLTFMHA KVLMFPATLT PSMSSQEQAD A
Length:931
Mass (Da):101,131
Last modified:March 29, 2005 - v2
Checksum:iFE1C64F3B7270620
GO
Isoform 2 (identifier: Q80WT5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     815-841: Missing.

Show »
Length:904
Mass (Da):98,490
Checksum:i506C3468B7BD598E
GO

Sequence cautioni

The sequence CAI24910.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAI25738.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei815 – 84127Missing in isoform 2. 1 PublicationVSP_013242Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL663115, AL645599 Genomic DNA. Translation: CAI25737.1.
AL663115, AL645599 Genomic DNA. Translation: CAI25738.1. Sequence problems.
AL645599, AL663115 Genomic DNA. Translation: CAI24909.1.
AL645599, AL663115 Genomic DNA. Translation: CAI24910.1. Sequence problems.
BC004630 mRNA. Translation: AAH04630.1.
BC052036 mRNA. Translation: AAH52036.1.
CCDSiCCDS24459.1. [Q80WT5-2]
CCDS70146.1. [Q80WT5-1]
RefSeqiNP_001239432.1. NM_001252503.2.
NP_001277474.1. NM_001290545.1. [Q80WT5-1]
NP_852076.1. NM_181411.4. [Q80WT5-2]
UniGeneiMm.356580.
Mm.489893.

Genome annotation databases

EnsembliENSMUST00000035350; ENSMUSP00000036778; ENSMUSG00000049659. [Q80WT5-2]
ENSMUST00000146722; ENSMUSP00000121612; ENSMUSG00000049659. [Q80WT5-1]
GeneIDi216549.
KEGGimmu:216549.
UCSCiuc007idh.2. mouse. [Q80WT5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL663115 , AL645599 Genomic DNA. Translation: CAI25737.1 .
AL663115 , AL645599 Genomic DNA. Translation: CAI25738.1 . Sequence problems.
AL645599 , AL663115 Genomic DNA. Translation: CAI24909.1 .
AL645599 , AL663115 Genomic DNA. Translation: CAI24910.1 . Sequence problems.
BC004630 mRNA. Translation: AAH04630.1 .
BC052036 mRNA. Translation: AAH52036.1 .
CCDSi CCDS24459.1. [Q80WT5-2 ]
CCDS70146.1. [Q80WT5-1 ]
RefSeqi NP_001239432.1. NM_001252503.2.
NP_001277474.1. NM_001290545.1. [Q80WT5-1 ]
NP_852076.1. NM_181411.4. [Q80WT5-2 ]
UniGenei Mm.356580.
Mm.489893.

3D structure databases

ProteinModelPortali Q80WT5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1344435.
STRINGi 10090.ENSMUSP00000091938.

PTM databases

PhosphoSitei Q80WT5.

Proteomic databases

MaxQBi Q80WT5.
PaxDbi Q80WT5.
PRIDEi Q80WT5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035350 ; ENSMUSP00000036778 ; ENSMUSG00000049659 . [Q80WT5-2 ]
ENSMUST00000146722 ; ENSMUSP00000121612 ; ENSMUSG00000049659 . [Q80WT5-1 ]
GeneIDi 216549.
KEGGi mmu:216549.
UCSCi uc007idh.2. mouse. [Q80WT5-2 ]

Organism-specific databases

CTDi 54812.
MGIi MGI:1923012. Aftph.

Phylogenomic databases

eggNOGi NOG40359.
GeneTreei ENSGT00390000017835.
HOGENOMi HOG000033840.
HOVERGENi HBG050464.
InParanoidi Q80WT5.
OMAi ETVNPQG.
OrthoDBi EOG779NX4.
PhylomeDBi Q80WT5.
TreeFami TF331532.

Miscellaneous databases

ChiTaRSi AFTPH. mouse.
NextBioi 375204.
PROi Q80WT5.
SOURCEi Search...

Gene expression databases

Bgeei Q80WT5.
CleanExi MM_AFTPH.
ExpressionAtlasi Q80WT5. baseline and differential.
Genevestigatori Q80WT5.

Family and domain databases

InterProi IPR029205. Clathrin-bd.
[Graphical view ]
Pfami PF15045. Clathrin_bdg. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Brain and Mammary gland.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAFTIN_MOUSE
AccessioniPrimary (citable) accession number: Q80WT5
Secondary accession number(s): Q5SSE6, Q99KJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: October 29, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3