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Q80WT5 (AFTIN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aftiphilin
Gene names
Name:Aftph
Synonyms:Afth
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length931 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in membrane trafficking By similarity.

Subunit structure

Interacts with GGA1, GGA3, AP1G1 and AP1G2 via their GAE domain By similarity.

Subcellular location

Cytoplasm By similarity. Note: Colocalizes with AP1G1 and clathrin By similarity.

Domain

The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself By similarity.

Sequence caution

The sequence CAI24910.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI25738.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   DomainRepeat
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentAP-1 adaptor complex

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q80WT5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q80WT5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     815-841: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 931931Aftiphilin
PRO_0000064489

Regions

Region821 – 8255Clathrin-binding Potential
Motif28 – 314WXXF motif 1
Motif433 – 4364WXXF motif 2
Motif476 – 4794WXXF motif 3

Amino acid modifications

Modified residue1511Phosphoserine By similarity
Modified residue3951Phosphoserine Ref.3 Ref.4
Modified residue6131Phosphothreonine By similarity

Natural variations

Alternative sequence815 – 84127Missing in isoform 2.
VSP_013242

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: FE1C64F3B7270620

FASTA931101,131
        10         20         30         40         50         60 
MEPDIIRMYS SSPPPLDNGA EDDEEDEFGE FGGFSEVSPS GVGFVDFDTP DYTRPKEDFV 

        70         80         90        100        110        120 
PSNHFMPIHE YSEDVDSLTS FKSVQNGNDK DITAELSTPV KSQSDVVLST TSKEMIPSKT 

       130        140        150        160        170        180 
LDPSIDGMES LEDLDKVVVQ GPSTGQLRSF SPGDFRTDKN IVHQTKQLES CNGEKPPCLE 

       190        200        210        220        230        240 
ILTNGFAGLE TVNPQGTDDL DNVADSKGSK PLNTCGTECI LESAASHATE FADFSTFSQT 

       250        260        270        280        290        300 
ERTQLEEIEC PVLNDGDTLT IQGNSKGPRV KELNCVKEVT LDGSFEDTGN TEREHQVCVS 

       310        320        330        340        350        360 
EIHAVADRGL SVEKQDLQTL QQDEFLNSRI QSEAWSLVDS SENSEAITKE RCKMEKNDLF 

       370        380        390        400        410        420 
ASKCADLSMD SVKTSDVNEI GSSKEENRKL TNPKSPDPDP TGQNALDDSA ASMKNGDSGN 

       430        440        450        460        470        480 
GFVTCHDTNE DDFGDFGTAN GTTPPFVTST QDSMSDVTFE DSSEHFLHLS EPGDDFGEFE 

       490        500        510        520        530        540 
DTNAVSCQEE MRFTESDLRQ TSDGLSEECP LAGESGGKDS KPDSKLKNGQ DSEFGDFDSV 

       550        560        570        580        590        600 
PNTQGSAFQD SDDFADFSSA GPSQAVDWNA FEDEQKDGCS WAAFGDQQET ESHHLKEVWQ 

       610        620        630        640        650        660 
SQRTDETMGT LGTPKMHSVS SAASKGAVAS GHLQEPGTSV QTALLNRLER IFEACFPSVF 

       670        680        690        700        710        720 
VPDVEEEVSS LKHLLETHSS PAKTREALAD RGELRGVWTE LQDIHDAHGL RYQWGGSHSN 

       730        740        750        760        770        780 
KKLLCSLGID TRNILFTGNK KQPVIVPMYA AGLGMLEPTK EPLKPLSAAE KIASIGQTTV 

       790        800        810        820        830        840 
MTPEINTCTS DPFQESLPPV QFDWSSSGLT NPLDASGGST LLNLDFFGPV DDSSSSSSTI 

       850        860        870        880        890        900 
PGVDPELYEL TTAKLETSTS SLRVTDAFAK LMSTVEKTST STRKPKREEH LSEEAMKVIA 

       910        920        930 
SLPDLTFMHA KVLMFPATLT PSMSSQEQAD A 

« Hide

Isoform 2 [UniParc].

Checksum: 506C3468B7BD598E
Show »

FASTA90498,490

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain and Mammary gland.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL663115, AL645599 Genomic DNA. Translation: CAI25737.1.
AL663115, AL645599 Genomic DNA. Translation: CAI25738.1. Sequence problems.
AL645599, AL663115 Genomic DNA. Translation: CAI24909.1.
AL645599, AL663115 Genomic DNA. Translation: CAI24910.1. Sequence problems.
BC004630 mRNA. Translation: AAH04630.1.
BC052036 mRNA. Translation: AAH52036.1.
CCDSCCDS24459.1. [Q80WT5-2]
RefSeqNP_001239432.1. NM_001252503.2.
NP_001277474.1. NM_001290545.1. [Q80WT5-1]
NP_852076.1. NM_181411.4. [Q80WT5-2]
UniGeneMm.356580.
Mm.489893.

3D structure databases

ProteinModelPortalQ80WT5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1344435.
STRING10090.ENSMUSP00000091938.

PTM databases

PhosphoSiteQ80WT5.

Proteomic databases

MaxQBQ80WT5.
PaxDbQ80WT5.
PRIDEQ80WT5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035350; ENSMUSP00000036778; ENSMUSG00000049659. [Q80WT5-2]
ENSMUST00000146722; ENSMUSP00000121612; ENSMUSG00000049659. [Q80WT5-1]
GeneID216549.
KEGGmmu:216549.
UCSCuc007idh.2. mouse. [Q80WT5-2]

Organism-specific databases

CTD54812.
MGIMGI:1923012. Aftph.

Phylogenomic databases

eggNOGNOG40359.
GeneTreeENSGT00390000017835.
HOGENOMHOG000033840.
HOVERGENHBG050464.
InParanoidQ80WT5.
OMAETVNPQG.
OrthoDBEOG779NX4.
PhylomeDBQ80WT5.
TreeFamTF331532.

Gene expression databases

ArrayExpressQ80WT5.
BgeeQ80WT5.
CleanExMM_AFTPH.
GenevestigatorQ80WT5.

Family and domain databases

InterProIPR029205. Clathrin-bd.
[Graphical view]
PfamPF15045. Clathrin_bdg. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAFTPH. mouse.
NextBio375204.
PROQ80WT5.
SOURCESearch...

Entry information

Entry nameAFTIN_MOUSE
AccessionPrimary (citable) accession number: Q80WT5
Secondary accession number(s): Q5SSE6, Q99KJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: July 9, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot