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Q80WT5

- AFTIN_MOUSE

UniProt

Q80WT5 - AFTIN_MOUSE

Protein

Aftiphilin

Gene

Aftph

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    May play a role in membrane trafficking.By similarity

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aftiphilin
    Gene namesi
    Name:Aftph
    Synonyms:Afth
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1923012. Aftph.

    Subcellular locationi

    Cytoplasm By similarity
    Note: Colocalizes with AP1G1 and clathrin.By similarity

    GO - Cellular componenti

    1. AP-1 adaptor complex Source: Ensembl
    2. cytosol Source: Ensembl
    3. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 931931AftiphilinPRO_0000064489Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei151 – 1511PhosphoserineBy similarity
    Modified residuei395 – 3951Phosphoserine2 Publications
    Modified residuei613 – 6131PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ80WT5.
    PaxDbiQ80WT5.
    PRIDEiQ80WT5.

    PTM databases

    PhosphoSiteiQ80WT5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ80WT5.
    BgeeiQ80WT5.
    CleanExiMM_AFTPH.
    GenevestigatoriQ80WT5.

    Interactioni

    Subunit structurei

    Interacts with GGA1, GGA3, AP1G1 and AP1G2 via their GAE domain.By similarity

    Protein-protein interaction databases

    MINTiMINT-1344435.
    STRINGi10090.ENSMUSP00000091938.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80WT5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni821 – 8255Clathrin-bindingSequence Analysis

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi28 – 314WXXF motif 1
    Motifi433 – 4364WXXF motif 2
    Motifi476 – 4794WXXF motif 3

    Domaini

    The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself By similarity.By similarity

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG40359.
    GeneTreeiENSGT00390000017835.
    HOGENOMiHOG000033840.
    HOVERGENiHBG050464.
    InParanoidiQ80WT5.
    OMAiETVNPQG.
    OrthoDBiEOG779NX4.
    PhylomeDBiQ80WT5.
    TreeFamiTF331532.

    Family and domain databases

    InterProiIPR029205. Clathrin-bd.
    [Graphical view]
    PfamiPF15045. Clathrin_bdg. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q80WT5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPDIIRMYS SSPPPLDNGA EDDEEDEFGE FGGFSEVSPS GVGFVDFDTP    50
    DYTRPKEDFV PSNHFMPIHE YSEDVDSLTS FKSVQNGNDK DITAELSTPV 100
    KSQSDVVLST TSKEMIPSKT LDPSIDGMES LEDLDKVVVQ GPSTGQLRSF 150
    SPGDFRTDKN IVHQTKQLES CNGEKPPCLE ILTNGFAGLE TVNPQGTDDL 200
    DNVADSKGSK PLNTCGTECI LESAASHATE FADFSTFSQT ERTQLEEIEC 250
    PVLNDGDTLT IQGNSKGPRV KELNCVKEVT LDGSFEDTGN TEREHQVCVS 300
    EIHAVADRGL SVEKQDLQTL QQDEFLNSRI QSEAWSLVDS SENSEAITKE 350
    RCKMEKNDLF ASKCADLSMD SVKTSDVNEI GSSKEENRKL TNPKSPDPDP 400
    TGQNALDDSA ASMKNGDSGN GFVTCHDTNE DDFGDFGTAN GTTPPFVTST 450
    QDSMSDVTFE DSSEHFLHLS EPGDDFGEFE DTNAVSCQEE MRFTESDLRQ 500
    TSDGLSEECP LAGESGGKDS KPDSKLKNGQ DSEFGDFDSV PNTQGSAFQD 550
    SDDFADFSSA GPSQAVDWNA FEDEQKDGCS WAAFGDQQET ESHHLKEVWQ 600
    SQRTDETMGT LGTPKMHSVS SAASKGAVAS GHLQEPGTSV QTALLNRLER 650
    IFEACFPSVF VPDVEEEVSS LKHLLETHSS PAKTREALAD RGELRGVWTE 700
    LQDIHDAHGL RYQWGGSHSN KKLLCSLGID TRNILFTGNK KQPVIVPMYA 750
    AGLGMLEPTK EPLKPLSAAE KIASIGQTTV MTPEINTCTS DPFQESLPPV 800
    QFDWSSSGLT NPLDASGGST LLNLDFFGPV DDSSSSSSTI PGVDPELYEL 850
    TTAKLETSTS SLRVTDAFAK LMSTVEKTST STRKPKREEH LSEEAMKVIA 900
    SLPDLTFMHA KVLMFPATLT PSMSSQEQAD A 931
    Length:931
    Mass (Da):101,131
    Last modified:March 29, 2005 - v2
    Checksum:iFE1C64F3B7270620
    GO
    Isoform 2 (identifier: Q80WT5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         815-841: Missing.

    Show »
    Length:904
    Mass (Da):98,490
    Checksum:i506C3468B7BD598E
    GO

    Sequence cautioni

    The sequence CAI24910.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAI25738.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei815 – 84127Missing in isoform 2. 1 PublicationVSP_013242Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL663115, AL645599 Genomic DNA. Translation: CAI25737.1.
    AL663115, AL645599 Genomic DNA. Translation: CAI25738.1. Sequence problems.
    AL645599, AL663115 Genomic DNA. Translation: CAI24909.1.
    AL645599, AL663115 Genomic DNA. Translation: CAI24910.1. Sequence problems.
    BC004630 mRNA. Translation: AAH04630.1.
    BC052036 mRNA. Translation: AAH52036.1.
    CCDSiCCDS24459.1. [Q80WT5-2]
    CCDS70146.1. [Q80WT5-1]
    RefSeqiNP_001239432.1. NM_001252503.2.
    NP_001277474.1. NM_001290545.1. [Q80WT5-1]
    NP_852076.1. NM_181411.4. [Q80WT5-2]
    UniGeneiMm.356580.
    Mm.489893.

    Genome annotation databases

    EnsembliENSMUST00000035350; ENSMUSP00000036778; ENSMUSG00000049659. [Q80WT5-2]
    ENSMUST00000146722; ENSMUSP00000121612; ENSMUSG00000049659. [Q80WT5-1]
    GeneIDi216549.
    KEGGimmu:216549.
    UCSCiuc007idh.2. mouse. [Q80WT5-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL663115 , AL645599 Genomic DNA. Translation: CAI25737.1 .
    AL663115 , AL645599 Genomic DNA. Translation: CAI25738.1 . Sequence problems.
    AL645599 , AL663115 Genomic DNA. Translation: CAI24909.1 .
    AL645599 , AL663115 Genomic DNA. Translation: CAI24910.1 . Sequence problems.
    BC004630 mRNA. Translation: AAH04630.1 .
    BC052036 mRNA. Translation: AAH52036.1 .
    CCDSi CCDS24459.1. [Q80WT5-2 ]
    CCDS70146.1. [Q80WT5-1 ]
    RefSeqi NP_001239432.1. NM_001252503.2.
    NP_001277474.1. NM_001290545.1. [Q80WT5-1 ]
    NP_852076.1. NM_181411.4. [Q80WT5-2 ]
    UniGenei Mm.356580.
    Mm.489893.

    3D structure databases

    ProteinModelPortali Q80WT5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1344435.
    STRINGi 10090.ENSMUSP00000091938.

    PTM databases

    PhosphoSitei Q80WT5.

    Proteomic databases

    MaxQBi Q80WT5.
    PaxDbi Q80WT5.
    PRIDEi Q80WT5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035350 ; ENSMUSP00000036778 ; ENSMUSG00000049659 . [Q80WT5-2 ]
    ENSMUST00000146722 ; ENSMUSP00000121612 ; ENSMUSG00000049659 . [Q80WT5-1 ]
    GeneIDi 216549.
    KEGGi mmu:216549.
    UCSCi uc007idh.2. mouse. [Q80WT5-2 ]

    Organism-specific databases

    CTDi 54812.
    MGIi MGI:1923012. Aftph.

    Phylogenomic databases

    eggNOGi NOG40359.
    GeneTreei ENSGT00390000017835.
    HOGENOMi HOG000033840.
    HOVERGENi HBG050464.
    InParanoidi Q80WT5.
    OMAi ETVNPQG.
    OrthoDBi EOG779NX4.
    PhylomeDBi Q80WT5.
    TreeFami TF331532.

    Miscellaneous databases

    ChiTaRSi AFTPH. mouse.
    NextBioi 375204.
    PROi Q80WT5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q80WT5.
    Bgeei Q80WT5.
    CleanExi MM_AFTPH.
    Genevestigatori Q80WT5.

    Family and domain databases

    InterProi IPR029205. Clathrin-bd.
    [Graphical view ]
    Pfami PF15045. Clathrin_bdg. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Brain and Mammary gland.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-395, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAFTIN_MOUSE
    AccessioniPrimary (citable) accession number: Q80WT5
    Secondary accession number(s): Q5SSE6, Q99KJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3