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Q80WQ8 (M18BP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mis18-binding protein 1
Alternative name(s):
Kinetochore-associated protein KNL-2 homolog
Gene names
Name:Mis18bp1
Synonyms:Kiaa1903, Knl2, M18bp1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length998 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis By similarity.

Subunit structure

Interacts with SP1 Probable. Interacts with MIS18A. Identified in a complex containing MIS18A, MIS18B, MIS18BP1, RBBP7 and RBBP4 By similarity. Interacts (via N-terminus) with FLNA (via N-terminus). Ref.4

Subcellular location

Nucleus By similarity. Chromosomecentromere By similarity. Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin By similarity.

Sequence similarities

Contains 1 SANT domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentCentromere
Chromosome
Nucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmitosis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchromosome, centromeric region

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

chromatin binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q80WQ8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q80WQ8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     433-434: AE → EN
     435-998: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q80WQ8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     433-998: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 998998Mis18-binding protein 1
PRO_0000379895

Regions

Domain741 – 79656SANT

Amino acid modifications

Modified residue1091Phosphoserine By similarity
Modified residue1341Phosphoserine By similarity
Modified residue1691Phosphoserine By similarity
Modified residue2581Phosphoserine By similarity
Modified residue5161Phosphothreonine By similarity
Modified residue6381Phosphoserine By similarity
Modified residue6391Phosphoserine By similarity
Modified residue6881Phosphothreonine By similarity
Modified residue7261Phosphoserine By similarity
Modified residue8721Phosphoserine By similarity
Modified residue9551Phosphoserine By similarity
Modified residue9851Phosphoserine By similarity

Natural variations

Alternative sequence433 – 998566Missing in isoform 3.
VSP_037731
Alternative sequence433 – 4342AE → EN in isoform 2.
VSP_037732
Alternative sequence435 – 998564Missing in isoform 2.
VSP_037733

Experimental info

Sequence conflict2261T → I in BAD90148. Ref.3
Sequence conflict3211T → A in BAC29410. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: E0194CC994543C8F

FASTA998113,956
        10         20         30         40         50         60 
MIVTPLKHSG IHLSSGTLQR RNMPLDAVFI DSIPSGTLTP LKDLVKYQKS SLKVNGHKKN 

        70         80         90        100        110        120 
QLLEIRTSNN KDLFQSTMLS EATLPNSSLD ISVIKPSMDR LRNEMIYESP GKIFQRMKAK 

       130        140        150        160        170        180 
VQRDKQEQLT RSSSMLGSPQ GEHTKDFPPN TDKKAQLQQT YICEEKQTSV QSNDPSLGDP 

       190        200        210        220        230        240 
PILNQEQKNV SASCISKKAL TRAQFGGQVL HSKESPVRIT VSKKNTFVLG GIDCTYEKFE 

       250        260        270        280        290        300 
NTDVNTISSL CVPIKNHSQS ITSDNDVTTE RTAKEDITEP NEEMMSRRTI LQDPIKNTSK 

       310        320        330        340        350        360 
IKRSSPRPNL TLSGRSQRKC TKLETVVKEV KKYQAVHLQE WMIKVINNNT AICVEGKLVD 

       370        380        390        400        410        420 
MTDVYWHSNV IIERIKHNEL RTLSGNIYIL KGLIDSVSMK EAGYPCYLTR KFMFGFPHNW 

       430        440        450        460        470        480 
KEHIDKFLEQ LRAEKKNKTR QETARVQEKQ KSKKKDAEDK ETYVLQKASI TYDLNDNSLE 

       490        500        510        520        530        540 
RTEVPTDPLN SLEQPTSGKE RRHPLLSQKR AYVLITPLRN KKLIEQRCID YSLSIEGISD 

       550        560        570        580        590        600 
FFKAKHQEES DSDIHGTPSS TSKSQETFEH RVGFEGNTKE DCNECDIITA RHIQIPCPKS 

       610        620        630        640        650        660 
KQMLTNDFMK KNKLPSKLQK TENQIGVSQY CRSSSHLSSE ENEVEIKSRT RARNTKERLN 

       670        680        690        700        710        720 
RERENTNHIT KDILLISETE GERACYITPK RPRSCYITPK RPRSSAKESH YKSAVSKDFL 

       730        740        750        760        770        780 
TEGKASDRTS RQLLDHLPGL TDDEEWSEQE LQKLHCAFTS LPKHKPGFWS DVAMAVGSRT 

       790        800        810        820        830        840 
ADECQKKYTE EPQGQGSRKH GSKKKQANKV QNGEKDSADA KTIKITAKVG TLKRKRQMRD 

       850        860        870        880        890        900 
CLEHLAKDNH DDFFTATPLQ KQRIQLPSFQ YSQDDDFLLD MDRDPASPSS IITSPLRSTT 

       910        920        930        940        950        960 
PQCQHFSPSM LAAIERNNCD RYVYQMQKNA KKYGKSNGGL VWGNIRKKTV KTDLSSPPPT 

       970        980        990 
RKALFNKDLG KNTDISKYFI DDTESDEEEK DYYFSNSD 

« Hide

Isoform 2 [UniParc].

Checksum: 40C837625C8B81CA
Show »

FASTA43449,324
Isoform 3 [UniParc].

Checksum: 67625C8B81CA3F1E
Show »

FASTA43249,080

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: C57BL/6J.
Tissue: Bone and Eye.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: FVB/N.
Tissue: Embryo and Mammary tumor.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 172-998 (ISOFORM 1).
[4]"Identification of novel nuclear protein interactions with the N-terminal part of filamin A."
Qiu H., Nomiyama R., Moriguchi K., Fukada T., Sugimoto K.
Biosci. Biotechnol. Biochem. 75:145-147(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FLNA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK036395 mRNA. Translation: BAC29410.1.
AK053496 mRNA. Translation: BAC35403.1.
AK162657 mRNA. Translation: BAE37008.1.
BC042728 mRNA. Translation: AAH42728.1.
BC052175 mRNA. Translation: AAH52175.1.
AK220223 mRNA. Translation: BAD90148.1.
RefSeqNP_766166.2. NM_172578.2.
UniGeneMm.21144.

3D structure databases

ProteinModelPortalQ80WQ8.
SMRQ80WQ8. Positions 739-800.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ80WQ8.

Proteomic databases

PRIDEQ80WQ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000052201; ENSMUSP00000052109; ENSMUSG00000047534. [Q80WQ8-1]
GeneID217653.
KEGGmmu:217653.
UCSCuc007nrg.2. mouse. [Q80WQ8-1]
uc007nri.1. mouse. [Q80WQ8-3]
uc011ymg.1. mouse. [Q80WQ8-2]

Organism-specific databases

CTD55320.
MGIMGI:2145099. Mis18bp1.
RougeSearch...

Phylogenomic databases

eggNOGNOG73415.
GeneTreeENSGT00390000007395.
InParanoidQ571G6.
OMANDCDKYV.
OrthoDBEOG77M8R3.
TreeFamTF106401.

Gene expression databases

ArrayExpressQ80WQ8.
BgeeQ80WQ8.
GenevestigatorQ80WQ8.

Family and domain databases

InterProIPR009057. Homeodomain-like.
IPR017877. Myb-like_dom.
IPR001005. SANT/Myb.
IPR015216. SANTA.
[Graphical view]
PfamPF09133. SANTA. 1 hit.
[Graphical view]
SMARTSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
ProtoNetSearch...

Other

NextBio375934.
PROQ80WQ8.
SOURCESearch...

Entry information

Entry nameM18BP_MOUSE
AccessionPrimary (citable) accession number: Q80WQ8
Secondary accession number(s): Q571G6 expand/collapse secondary AC list , Q8BPR7, Q8CBB5, Q8CG92
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2003
Last modified: March 19, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot