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Protein

Mis18-binding protein 1

Gene

Mis18bp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Names & Taxonomyi

Protein namesi
Recommended name:
Mis18-binding protein 1
Alternative name(s):
Kinetochore-associated protein KNL-2 homolog
Gene namesi
Name:Mis18bp1
Synonyms:Kiaa1903, Knl2, M18bp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2145099. Mis18bp1.

Subcellular locationi

  • Nucleus By similarity
  • Chromosomecentromere By similarity

  • Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003798951 – 998Mis18-binding protein 1Add BLAST998

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineBy similarity1
Modified residuei109PhosphoserineBy similarity1
Modified residuei134PhosphoserineBy similarity1
Modified residuei169PhosphoserineBy similarity1
Modified residuei258PhosphoserineBy similarity1
Modified residuei516PhosphothreonineCombined sources1
Modified residuei578PhosphothreonineCombined sources1
Modified residuei638PhosphoserineBy similarity1
Modified residuei639PhosphoserineBy similarity1
Modified residuei688PhosphothreonineBy similarity1
Modified residuei726PhosphoserineBy similarity1
Cross-linki765Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei872PhosphoserineBy similarity1
Modified residuei955PhosphoserineBy similarity1
Modified residuei985PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ80WQ8.
PaxDbiQ80WQ8.
PeptideAtlasiQ80WQ8.
PRIDEiQ80WQ8.

PTM databases

iPTMnetiQ80WQ8.
PhosphoSitePlusiQ80WQ8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000047534.
GenevisibleiQ80WQ8. MM.

Interactioni

Subunit structurei

Interacts with SP1 (Probable). Interacts with MIS18A. Identified in a complex containing MIS18A, OIP5/MIS18B, MIS18BP1, RBBP7 and RBBP4 (By similarity). Interacts (via N-terminus) with FLNA (via N-terminus).By similarityCurated1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052109.

Structurei

3D structure databases

ProteinModelPortaliQ80WQ8.
SMRiQ80WQ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini741 – 796SANTAdd BLAST56

Sequence similaritiesi

Contains 1 SANT domain.Curated

Phylogenomic databases

eggNOGiENOG410II9U. Eukaryota.
ENOG41120B5. LUCA.
GeneTreeiENSGT00390000007395.
InParanoidiQ80WQ8.
OMAiNDCDKYV.
OrthoDBiEOG091G050V.
TreeFamiTF106401.

Family and domain databases

InterProiIPR009057. Homeodomain-like.
IPR017877. Myb-like_dom.
IPR001005. SANT/Myb.
IPR015216. SANTA.
[Graphical view]
PfamiPF00249. Myb_DNA-binding. 1 hit.
PF09133. SANTA. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80WQ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIVTPLKHSG IHLSSGTLQR RNMPLDAVFI DSIPSGTLTP LKDLVKYQKS
60 70 80 90 100
SLKVNGHKKN QLLEIRTSNN KDLFQSTMLS EATLPNSSLD ISVIKPSMDR
110 120 130 140 150
LRNEMIYESP GKIFQRMKAK VQRDKQEQLT RSSSMLGSPQ GEHTKDFPPN
160 170 180 190 200
TDKKAQLQQT YICEEKQTSV QSNDPSLGDP PILNQEQKNV SASCISKKAL
210 220 230 240 250
TRAQFGGQVL HSKESPVRIT VSKKNTFVLG GIDCTYEKFE NTDVNTISSL
260 270 280 290 300
CVPIKNHSQS ITSDNDVTTE RTAKEDITEP NEEMMSRRTI LQDPIKNTSK
310 320 330 340 350
IKRSSPRPNL TLSGRSQRKC TKLETVVKEV KKYQAVHLQE WMIKVINNNT
360 370 380 390 400
AICVEGKLVD MTDVYWHSNV IIERIKHNEL RTLSGNIYIL KGLIDSVSMK
410 420 430 440 450
EAGYPCYLTR KFMFGFPHNW KEHIDKFLEQ LRAEKKNKTR QETARVQEKQ
460 470 480 490 500
KSKKKDAEDK ETYVLQKASI TYDLNDNSLE RTEVPTDPLN SLEQPTSGKE
510 520 530 540 550
RRHPLLSQKR AYVLITPLRN KKLIEQRCID YSLSIEGISD FFKAKHQEES
560 570 580 590 600
DSDIHGTPSS TSKSQETFEH RVGFEGNTKE DCNECDIITA RHIQIPCPKS
610 620 630 640 650
KQMLTNDFMK KNKLPSKLQK TENQIGVSQY CRSSSHLSSE ENEVEIKSRT
660 670 680 690 700
RARNTKERLN RERENTNHIT KDILLISETE GERACYITPK RPRSCYITPK
710 720 730 740 750
RPRSSAKESH YKSAVSKDFL TEGKASDRTS RQLLDHLPGL TDDEEWSEQE
760 770 780 790 800
LQKLHCAFTS LPKHKPGFWS DVAMAVGSRT ADECQKKYTE EPQGQGSRKH
810 820 830 840 850
GSKKKQANKV QNGEKDSADA KTIKITAKVG TLKRKRQMRD CLEHLAKDNH
860 870 880 890 900
DDFFTATPLQ KQRIQLPSFQ YSQDDDFLLD MDRDPASPSS IITSPLRSTT
910 920 930 940 950
PQCQHFSPSM LAAIERNNCD RYVYQMQKNA KKYGKSNGGL VWGNIRKKTV
960 970 980 990
KTDLSSPPPT RKALFNKDLG KNTDISKYFI DDTESDEEEK DYYFSNSD
Length:998
Mass (Da):113,956
Last modified:June 1, 2003 - v1
Checksum:iE0194CC994543C8F
GO
Isoform 2 (identifier: Q80WQ8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     433-434: AE → EN
     435-998: Missing.

Note: No experimental confirmation available.
Show »
Length:434
Mass (Da):49,324
Checksum:i40C837625C8B81CA
GO
Isoform 3 (identifier: Q80WQ8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     433-998: Missing.

Show »
Length:432
Mass (Da):49,080
Checksum:i67625C8B81CA3F1E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti226T → I in BAD90148 (Ref. 3) Curated1
Sequence conflicti321T → A in BAC29410 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037731433 – 998Missing in isoform 3. 1 PublicationAdd BLAST566
Alternative sequenceiVSP_037732433 – 434AE → EN in isoform 2. 1 Publication2
Alternative sequenceiVSP_037733435 – 998Missing in isoform 2. 1 PublicationAdd BLAST564

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036395 mRNA. Translation: BAC29410.1.
AK053496 mRNA. Translation: BAC35403.1.
AK162657 mRNA. Translation: BAE37008.1.
BC042728 mRNA. Translation: AAH42728.1.
BC052175 mRNA. Translation: AAH52175.1.
AK220223 mRNA. Translation: BAD90148.1.
CCDSiCCDS25942.1. [Q80WQ8-1]
RefSeqiNP_766166.2. NM_172578.2. [Q80WQ8-1]
UniGeneiMm.21144.

Genome annotation databases

EnsembliENSMUST00000052201; ENSMUSP00000052109; ENSMUSG00000047534. [Q80WQ8-1]
GeneIDi217653.
KEGGimmu:217653.
UCSCiuc007nrg.2. mouse. [Q80WQ8-1]
uc007nri.1. mouse. [Q80WQ8-3]
uc011ymg.1. mouse. [Q80WQ8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036395 mRNA. Translation: BAC29410.1.
AK053496 mRNA. Translation: BAC35403.1.
AK162657 mRNA. Translation: BAE37008.1.
BC042728 mRNA. Translation: AAH42728.1.
BC052175 mRNA. Translation: AAH52175.1.
AK220223 mRNA. Translation: BAD90148.1.
CCDSiCCDS25942.1. [Q80WQ8-1]
RefSeqiNP_766166.2. NM_172578.2. [Q80WQ8-1]
UniGeneiMm.21144.

3D structure databases

ProteinModelPortaliQ80WQ8.
SMRiQ80WQ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052109.

PTM databases

iPTMnetiQ80WQ8.
PhosphoSitePlusiQ80WQ8.

Proteomic databases

EPDiQ80WQ8.
PaxDbiQ80WQ8.
PeptideAtlasiQ80WQ8.
PRIDEiQ80WQ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052201; ENSMUSP00000052109; ENSMUSG00000047534. [Q80WQ8-1]
GeneIDi217653.
KEGGimmu:217653.
UCSCiuc007nrg.2. mouse. [Q80WQ8-1]
uc007nri.1. mouse. [Q80WQ8-3]
uc011ymg.1. mouse. [Q80WQ8-2]

Organism-specific databases

CTDi55320.
MGIiMGI:2145099. Mis18bp1.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410II9U. Eukaryota.
ENOG41120B5. LUCA.
GeneTreeiENSGT00390000007395.
InParanoidiQ80WQ8.
OMAiNDCDKYV.
OrthoDBiEOG091G050V.
TreeFamiTF106401.

Enzyme and pathway databases

ReactomeiR-MMU-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Miscellaneous databases

PROiQ80WQ8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047534.
GenevisibleiQ80WQ8. MM.

Family and domain databases

InterProiIPR009057. Homeodomain-like.
IPR017877. Myb-like_dom.
IPR001005. SANT/Myb.
IPR015216. SANTA.
[Graphical view]
PfamiPF00249. Myb_DNA-binding. 1 hit.
PF09133. SANTA. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiM18BP_MOUSE
AccessioniPrimary (citable) accession number: Q80WQ8
Secondary accession number(s): Q571G6
, Q8BPR7, Q8CBB5, Q8CG92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.