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Q80WN9

- B4GT2_CRIGR

UniProt

Q80WN9 - B4GT2_CRIGR

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Protein

Beta-1,4-galactosyltransferase 2

Gene
B4GALT2
Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose By similarity.

Catalytic activityi

UDP-alpha-D-galactose + D-glucose = UDP + lactose.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi215 – 2151Manganese By similarity
Binding sitei275 – 2751UDP-alpha-D-galactose By similarity
Metal bindingi308 – 3081Manganese; via tele nitrogen By similarity
Binding sitei320 – 3201N-acetyl-D-glucosamine By similarity

GO - Molecular functioni

  1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
  2. lactose synthase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. N-acetyllactosamine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 2 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 2
Short name:
Beta4Gal-T2
Short name:
b4Gal-T2
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2
Including the following 4 domains:
Lactose synthase A protein (EC:2.4.1.22)
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4GALT2
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity
Note: Trans cisternae of Golgi stack By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei16 – 3621Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini37 – 369333Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Beta-1,4-galactosyltransferase 2PRO_0000080532Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi68 – 681N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi94 ↔ 136 By similarity
Disulfide bondi208 ↔ 227 By similarity
Glycosylationi354 – 3541N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ80WN9.
SMRiQ80WN9. Positions 94-362.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni147 – 1515UDP-alpha-D-galactose binding By similarity
Regioni186 – 1883UDP-alpha-D-galactose binding By similarity
Regioni214 – 2152UDP-alpha-D-galactose binding By similarity
Regioni277 – 2804N-acetyl-D-glucosamine binding By similarity
Regioni308 – 3103UDP-alpha-D-galactose binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG058334.
KOiK07967.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q80WN9-1 [UniParc]FASTAAdd to Basket

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MSRLLGGTLE RVCKAVLLLC LLHFLVAVIL YFDVYAQHLA FFSRFSIRSP    50
AHALYPAASS STNCSRPNAT ASSSGLPEVP SARPGPTAPV IPPCPDVPPG 100
LVGRVVIEFT SPMPLERVQR ENPGVLLGGR YTPPDCTPAQ TVAVIIPFRH 150
REHHLRYWLH YLHPMLRRQR LRYGIYVINQ HGEETFNRAK LLNVGFLEAL 200
KEDATYDCFI FSDVDLVPMD DRNLYRCGDQ PRHFAIAMDK FGFRLPYASY 250
FGGVSGLSKA QFLRINGFPN EYWGWGGEDD DIFNRISLTG MKISRPDVRI 300
GRYRMIKHDR DKHNEPNPQR FSKIQNTKLS MKWDGIGSLR YRVLEVSRQP 350
LFTNITVDIG RPMSWLNQG 369
Length:369
Mass (Da):42,005
Last modified:June 1, 2003 - v1
Checksum:i7577A87D31BFDB04
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY117536 mRNA. Translation: AAM77195.1.
RefSeqiNP_001233623.1. NM_001246694.2.
XP_007641467.1. XM_007643277.1.

Genome annotation databases

GeneIDi100689434.
KEGGicge:100689434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY117536 mRNA. Translation: AAM77195.1 .
RefSeqi NP_001233623.1. NM_001246694.2.
XP_007641467.1. XM_007643277.1.

3D structure databases

ProteinModelPortali Q80WN9.
SMRi Q80WN9. Positions 94-362.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100689434.
KEGGi cge:100689434.

Organism-specific databases

CTDi 8704.

Phylogenomic databases

HOVERGENi HBG058334.
KOi K07967.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A mutation causing a reduced level of expression of six beta4-galactosyltransferase genes is the basis of the Lec19 CHO glycosylation mutant."
    Lee J., Park S.-H., Sundaram S., Raju T.S., Shaper N.L., Stanley P.
    Biochemistry 42:12349-12357(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiB4GT2_CRIGR
AccessioniPrimary (citable) accession number: Q80WN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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