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Q80WN9 (B4GT2_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified October 31, 2012. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-1,4-galactosyltransferase 2
Short name=Beta-1,4-GalTase 2
Short name=Beta4Gal-T2
Short name=b4Gal-T2
EC=2.4.1.-
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2

Including the following 4 domains:

  1. Lactose synthase A protein
    EC=2.4.1.22
  2. N-acetyllactosamine synthase
    EC=2.4.1.90
    Alternative name(s):
    Nal synthase
  3. Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
    EC=2.4.1.38
  4. Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
    EC=2.4.1.-
Gene names
Name:B4GALT2
OrganismCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose By similarity.

Catalytic activity

UDP-alpha-D-galactose + D-glucose = UDP + lactose.

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.

Sequence similarities

Belongs to the glycosyltransferase 7 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Beta-1,4-galactosyltransferase 2
PRO_0000080532

Regions

Topological domain1 – 1515Cytoplasmic Potential
Transmembrane16 – 3621Helical; Signal-anchor for type II membrane protein; Potential
Topological domain37 – 369333Lumenal Potential

Sites

Metal binding2151Manganese By similarity
Metal binding3081Manganese By similarity

Amino acid modifications

Glycosylation631N-linked (GlcNAc...) Potential
Glycosylation681N-linked (GlcNAc...) Potential
Glycosylation3541N-linked (GlcNAc...) Potential
Disulfide bond94 ↔ 136 By similarity
Disulfide bond208 ↔ 227 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q80WN9 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 7577A87D31BFDB04

FASTA36942,005
        10         20         30         40         50         60 
MSRLLGGTLE RVCKAVLLLC LLHFLVAVIL YFDVYAQHLA FFSRFSIRSP AHALYPAASS 

        70         80         90        100        110        120 
STNCSRPNAT ASSSGLPEVP SARPGPTAPV IPPCPDVPPG LVGRVVIEFT SPMPLERVQR 

       130        140        150        160        170        180 
ENPGVLLGGR YTPPDCTPAQ TVAVIIPFRH REHHLRYWLH YLHPMLRRQR LRYGIYVINQ 

       190        200        210        220        230        240 
HGEETFNRAK LLNVGFLEAL KEDATYDCFI FSDVDLVPMD DRNLYRCGDQ PRHFAIAMDK 

       250        260        270        280        290        300 
FGFRLPYASY FGGVSGLSKA QFLRINGFPN EYWGWGGEDD DIFNRISLTG MKISRPDVRI 

       310        320        330        340        350        360 
GRYRMIKHDR DKHNEPNPQR FSKIQNTKLS MKWDGIGSLR YRVLEVSRQP LFTNITVDIG 


RPMSWLNQG

« Hide

References

[1]"A mutation causing a reduced level of expression of six beta4-galactosyltransferase genes is the basis of the Lec19 CHO glycosylation mutant."
Lee J., Park S.-H., Sundaram S., Raju T.S., Shaper N.L., Stanley P.
Biochemistry 42:12349-12357(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY117536 mRNA. Translation: AAM77195.1.
RefSeqNP_001233623.1. NM_001246694.2.

3D structure databases

ProteinModelPortalQ80WN9.
SMRQ80WN9. Positions 94-362.
ModBaseSearch...

Protein family/group databases

CAZyGT7. Glycosyltransferase Family 7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100689434.

Organism-specific databases

CTD8704.

Phylogenomic databases

HOVERGENHBG058334.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR003859. Galactosyl_T_2_met.
[Graphical view]
PANTHERPTHR19300. PTHR19300. 1 hit.
PfamPF02709. Glyco_transf_7C. 1 hit.
[Graphical view]
PRINTSPR02050. B14GALTRFASE.
ProtoNetSearch...

Entry information

Entry nameB4GT2_CRIGR
AccessionPrimary (citable) accession number: Q80WN9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2003
Last modified: October 31, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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