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Q80WN9

- B4GT2_CRIGR

UniProt

Q80WN9 - B4GT2_CRIGR

Protein

Beta-1,4-galactosyltransferase 2

Gene

B4GALT2

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose By similarity.By similarity

    Catalytic activityi

    UDP-alpha-D-galactose + D-glucose = UDP + lactose.
    UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
    UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi215 – 2151ManganeseBy similarity
    Binding sitei275 – 2751UDP-alpha-D-galactoseBy similarity
    Metal bindingi308 – 3081Manganese; via tele nitrogenBy similarity
    Binding sitei320 – 3201N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
    2. lactose synthase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. N-acetyllactosamine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 2 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 2
    Short name:
    Beta4Gal-T2
    Short name:
    b4Gal-T2
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2
    Including the following 4 domains:
    Lactose synthase A protein (EC:2.4.1.22)
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
    Gene namesi
    Name:B4GALT2
    OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
    Taxonomic identifieri10029 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

    Subcellular locationi

    Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Trans cisternae of Golgi stack.By similarity

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Beta-1,4-galactosyltransferase 2PRO_0000080532Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi68 – 681N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi94 ↔ 136By similarity
    Disulfide bondi208 ↔ 227By similarity
    Glycosylationi354 – 3541N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ80WN9.
    SMRiQ80WN9. Positions 94-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini37 – 369333LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni147 – 1515UDP-alpha-D-galactose bindingBy similarity
    Regioni186 – 1883UDP-alpha-D-galactose bindingBy similarity
    Regioni214 – 2152UDP-alpha-D-galactose bindingBy similarity
    Regioni277 – 2804N-acetyl-D-glucosamine bindingBy similarity
    Regioni308 – 3103UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG058334.
    KOiK07967.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q80WN9-1 [UniParc]FASTAAdd to Basket

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    MSRLLGGTLE RVCKAVLLLC LLHFLVAVIL YFDVYAQHLA FFSRFSIRSP    50
    AHALYPAASS STNCSRPNAT ASSSGLPEVP SARPGPTAPV IPPCPDVPPG 100
    LVGRVVIEFT SPMPLERVQR ENPGVLLGGR YTPPDCTPAQ TVAVIIPFRH 150
    REHHLRYWLH YLHPMLRRQR LRYGIYVINQ HGEETFNRAK LLNVGFLEAL 200
    KEDATYDCFI FSDVDLVPMD DRNLYRCGDQ PRHFAIAMDK FGFRLPYASY 250
    FGGVSGLSKA QFLRINGFPN EYWGWGGEDD DIFNRISLTG MKISRPDVRI 300
    GRYRMIKHDR DKHNEPNPQR FSKIQNTKLS MKWDGIGSLR YRVLEVSRQP 350
    LFTNITVDIG RPMSWLNQG 369
    Length:369
    Mass (Da):42,005
    Last modified:June 1, 2003 - v1
    Checksum:i7577A87D31BFDB04
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY117536 mRNA. Translation: AAM77195.1.
    RefSeqiNP_001233623.1. NM_001246694.2.
    XP_007641467.1. XM_007643277.1.

    Genome annotation databases

    GeneIDi100689434.
    KEGGicge:100689434.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY117536 mRNA. Translation: AAM77195.1 .
    RefSeqi NP_001233623.1. NM_001246694.2.
    XP_007641467.1. XM_007643277.1.

    3D structure databases

    ProteinModelPortali Q80WN9.
    SMRi Q80WN9. Positions 94-362.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100689434.
    KEGGi cge:100689434.

    Organism-specific databases

    CTDi 8704.

    Phylogenomic databases

    HOVERGENi HBG058334.
    KOi K07967.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A mutation causing a reduced level of expression of six beta4-galactosyltransferase genes is the basis of the Lec19 CHO glycosylation mutant."
      Lee J., Park S.-H., Sundaram S., Raju T.S., Shaper N.L., Stanley P.
      Biochemistry 42:12349-12357(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiB4GT2_CRIGR
    AccessioniPrimary (citable) accession number: Q80WN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3