Reviewed, UniProtKB/Swiss-Prot Q80WN9 (B4GT2_CRIGR)

Names and origin

Protein names

Recommended name:
    Beta-1,4-galactosyltransferase 2
      Short name=Beta-1,4-GalTase 2
      Short name=Beta4Gal-T2
      Short name=b4Gal-T2
    EC=2.4.1.-
Alternative name(s):
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2
Including the following 4 domains:
    1- Recommended name:
            Lactose synthase A protein
              EC=2.4.1.22
    2- Recommended name:
            N-acetyllactosamine synthase
              EC=2.4.1.90
        Alternative name(s):
            Nal synthetase
    3- Recommended name:
            Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
              EC=2.4.1.38
    4- Recommended name:
            Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
              EC=2.4.1.-

Gene names

Name:

B4GALT2

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose By similarity.
Catalytic activity	UDP-galactose + D-glucose = UDP + lactose. UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide. UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosamine synthase activity Inferred from electronic annotation. Source: EC beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Inferred from electronic annotation. Source: EC lactose synthase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Beta-1,4-galactosyltransferase 2		PRO_0000080532
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 36	21	Signal-anchor for type II membrane protein Potential
	Topological domain	37 – 369	333	Lumenal Potential
Sites
	Metal binding	215	1	Manganese By similarity
	Metal binding	308	1	Manganese By similarity
Amino acid modifications
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	354	1	N-linked (GlcNAc...) Potential
	Disulfide bond	94 ↔ 136		By similarity
	Disulfide bond	208 ↔ 227		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q80WN9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7577A87D31BFDB04	FASTA	369	42,005
	10 20 30 40 50 60 MSRLLGGTLE RVCKAVLLLC LLHFLVAVIL YFDVYAQHLA FFSRFSIRSP AHALYPAASS 70 80 90 100 110 120 STNCSRPNAT ASSSGLPEVP SARPGPTAPV IPPCPDVPPG LVGRVVIEFT SPMPLERVQR 130 140 150 160 170 180 ENPGVLLGGR YTPPDCTPAQ TVAVIIPFRH REHHLRYWLH YLHPMLRRQR LRYGIYVINQ 190 200 210 220 230 240 HGEETFNRAK LLNVGFLEAL KEDATYDCFI FSDVDLVPMD DRNLYRCGDQ PRHFAIAMDK 250 260 270 280 290 300 FGFRLPYASY FGGVSGLSKA QFLRINGFPN EYWGWGGEDD DIFNRISLTG MKISRPDVRI 310 320 330 340 350 360 GRYRMIKHDR DKHNEPNPQR FSKIQNTKLS MKWDGIGSLR YRVLEVSRQP LFTNITVDIG RPMSWLNQG

References

[1]

"A mutation causing a reduced level of expression of six beta4-galactosyltransferase genes is the basis of the Lec19 CHO glycosylation mutant." Lee J., Park S.-H., Sundaram S., Raju T.S., Shaper N.L., Stanley P. Biochemistry 42:12349-12357(2003) [PubMed: 14567696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY117536 mRNA. Translation: AAM77195.1.
3D structure databases
SMR	Q80WN9. Positions 94-362.
ModBase	Search...
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Phylogenomic databases
HOVERGEN	Q80WN9.
Enzyme and pathway databases
BRENDA	2.4.1.22. 18. 2.4.1.38. 18. 2.4.1.90. 18.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2. [Graphical view]
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B4GT2_CRIGR

Accession

Primary (citable) accession number: Q80WN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)