Reviewed, UniProtKB/Swiss-Prot Q80WN8 (B4GT3_CRIGR)

Names and origin

Protein names

Recommended name:
    Beta-1,4-galactosyltransferase 3
      Short name=Beta-1,4-GalTase 3
      Short name=Beta4Gal-T3
      Short name=b4Gal-T3
    EC=2.4.1.-
Alternative name(s):
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
Including the following 3 domains:
    1- Recommended name:
            N-acetyllactosamine synthase
              EC=2.4.1.90
        Alternative name(s):
            Nal synthetase
    2- Recommended name:
            Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
              EC=2.4.1.38
    3- Recommended name:
            Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
              EC=2.4.1.-

Gene names

Name:

B4GALT3

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.
Catalytic activity	UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide. UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetyllactosamine synthase activity Inferred from electronic annotation. Source: EC beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Beta-1,4-galactosyltransferase 3		PRO_0000080536
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Signal-anchor for type II membrane protein Potential
	Topological domain	32 – 395	364	Lumenal Potential
Sites
	Metal binding	199	1	Manganese By similarity
	Metal binding	293	1	Manganese By similarity
Amino acid modifications
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
	Glycosylation	339	1	N-linked (GlcNAc...) Potential
	Glycosylation	387	1	N-linked (GlcNAc...) Potential
	Disulfide bond	79 ↔ 121		By similarity
	Disulfide bond	192 ↔ 211		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q80WN8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7FB9760DC6329680	FASTA	395	44,060
	10 20 30 40 50 60 MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DQGPTFDYSH PRDVYSNLSH 70 80 90 100 110 120 LPGAPVAAGA SPAEALPFCP ERSPFLVGPV SVSFSPVPSL AEIVERNPRV EPGGRYRPAG 130 140 150 160 170 180 CEARSRTAII VPHRAREHHL RLLLYHLHPF LQRQQLAYGI YVIHQAGNGM FNRAKLLNVG 190 200 210 220 230 240 VREALRDEEW DCLFLHDVDL LPENDHNLYV CDPRGPRHVA VAMNKFGYSL PYPQYFGGVS 250 260 270 280 290 300 ALTPDQYLKM NGFPNEYWGW GGEDDDIATR VRLAGMKISR PPTSVGHYKM VKHRGDKGNE 310 320 330 340 350 360 ENPHRFDLLV RTQNSWTQDG MNSLTYQLLA KELGPLYTNI TADIGTDPRG PRAPSGPRYP 370 380 390 PGSSQAFRQE MLQRRPPARP GPLPTANHTA PHGSH

References

[1]

"A mutation causing a reduced level of expression of six beta4-galactosyltransferase genes is the basis of the Lec19 CHO glycosylation mutant." Lee J., Park S.-H., Sundaram S., Raju T.S., Shaper N.L., Stanley P. Biochemistry 42:12349-12357(2003) [PubMed: 14567696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY117537 mRNA. Translation: AAM77196.1.
3D structure databases
HSSP	HSSP built from PDB template 1FGX based on UniProtKB P08037.
ModBase	Search...
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Phylogenomic databases
HOVERGEN	Q80WN8.
Enzyme and pathway databases
BRENDA	2.4.1.38. 18. 2.4.1.90. 18.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2. [Graphical view]
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
Pfam	PF02709. Galactosyl_T_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B4GT3_CRIGR

Accession

Primary (citable) accession number: Q80WN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)