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Protein

Beta-1,4-galactosyltransferase 4

Gene

B4GALT4

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi196 – 1961ManganeseBy similarity
Binding sitei224 – 2241UDP-alpha-D-galactoseBy similarity
Binding sitei256 – 2561UDP-alpha-D-galactoseBy similarity
Metal bindingi289 – 2891Manganese; via tele nitrogenBy similarity
Binding sitei301 – 3011N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 4 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 4
Short name:
Beta4Gal-T4
Short name:
b4Gal-T4
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
Including the following 2 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Lactotriaosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.275)
Alternative name(s):
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
Gene namesi
Name:B4GALT4
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3826Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini39 – 344306LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Beta-1,4-galactosyltransferase 4PRO_0000080541Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi77 ↔ 118By similarity
Disulfide bondi189 ↔ 208By similarity
Glycosylationi220 – 2201N-linked (GlcNAc...)Sequence analysis
Glycosylationi335 – 3351N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ80WN7.
SMRiQ80WN7. Positions 74-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1335UDP-alpha-D-galactose bindingBy similarity
Regioni168 – 1703UDP-alpha-D-galactose bindingBy similarity
Regioni195 – 1962UDP-alpha-D-galactose bindingBy similarity
Regioni258 – 2614N-acetyl-D-glucosamine bindingBy similarity
Regioni289 – 2913UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG058334.
KOiK07969.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q80WN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCNPPYLLP YRLRLLLFFT LCLTVVGWVT SNYFVDPIQV IPKAKVFMAS
60 70 80 90 100
FYKVIPLGKE ETLVHDATME KVELGNCPSV SPNLRGQSKL IFEPDLTLEE
110 120 130 140 150
VQAKNPKVSG GRYHPEECKA VQRVAVLIPH RNREKHLTYL LEHLHPFLQR
160 170 180 190 200
QQLDYGIYII HQTGSKKFNR AKLLNVGYLE ALKEQNWDCF IFHDVDLVPE
210 220 230 240 250
NDFNLYTCGD QPKHLVVGRN STGYRLRYSK YFGGVTALSR EQFFKVNGFS
260 270 280 290 300
NNYWGWGGED DDLRLRVELH KMKISRPNPD VGKYTMIFHT RDKGNEVNVD
310 320 330 340
RMKLLHQMSR VWKTDGLSSC SYRLLSVEHN PLYTNITVDF WTGV
Length:344
Mass (Da):39,893
Last modified:June 1, 2003 - v1
Checksum:i23AA84AB3187E53E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY117538 mRNA. Translation: AAM77197.1.
RefSeqiNP_001233624.1. NM_001246695.1.
XP_007647946.1. XM_007649756.1.
XP_007647947.1. XM_007649757.1.
XP_007647948.1. XM_007649758.1.

Genome annotation databases

GeneIDi100689435.
KEGGicge:100689435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY117538 mRNA. Translation: AAM77197.1.
RefSeqiNP_001233624.1. NM_001246695.1.
XP_007647946.1. XM_007649756.1.
XP_007647947.1. XM_007649757.1.
XP_007647948.1. XM_007649758.1.

3D structure databases

ProteinModelPortaliQ80WN7.
SMRiQ80WN7. Positions 74-342.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100689435.
KEGGicge:100689435.

Organism-specific databases

CTDi8702.

Phylogenomic databases

HOVERGENiHBG058334.
KOiK07969.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A mutation causing a reduced level of expression of six beta4-galactosyltransferase genes is the basis of the Lec19 CHO glycosylation mutant."
    Lee J., Park S.-H., Sundaram S., Raju T.S., Shaper N.L., Stanley P.
    Biochemistry 42:12349-12357(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiB4GT4_CRIGR
AccessioniPrimary (citable) accession number: Q80WN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2003
Last modified: October 14, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.