Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-1,4-galactosyltransferase 4

Gene

B4GALT4

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi196ManganeseBy similarity1
Binding sitei224UDP-alpha-D-galactoseBy similarity1
Binding sitei256UDP-alpha-D-galactoseBy similarity1
Metal bindingi289Manganese; via tele nitrogenBy similarity1
Binding sitei301N-acetyl-D-glucosamineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 4 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 4
Short name:
Beta4Gal-T4
Short name:
b4Gal-T4
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
Including the following 2 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Lactotriaosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.275)
Alternative name(s):
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
Gene namesi
Name:B4GALT4
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 38Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST26
Topological domaini39 – 344LumenalSequence analysisAdd BLAST306

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805411 – 344Beta-1,4-galactosyltransferase 4Add BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi77 ↔ 118By similarity
Disulfide bondi189 ↔ 208By similarity
Glycosylationi220N-linked (GlcNAc...)Sequence analysis1
Glycosylationi335N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ80WN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 133UDP-alpha-D-galactose bindingBy similarity5
Regioni168 – 170UDP-alpha-D-galactose bindingBy similarity3
Regioni195 – 196UDP-alpha-D-galactose bindingBy similarity2
Regioni258 – 261N-acetyl-D-glucosamine bindingBy similarity4
Regioni289 – 291UDP-alpha-D-galactose bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG058334.
KOiK07969.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q80WN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCNPPYLLP YRLRLLLFFT LCLTVVGWVT SNYFVDPIQV IPKAKVFMAS
60 70 80 90 100
FYKVIPLGKE ETLVHDATME KVELGNCPSV SPNLRGQSKL IFEPDLTLEE
110 120 130 140 150
VQAKNPKVSG GRYHPEECKA VQRVAVLIPH RNREKHLTYL LEHLHPFLQR
160 170 180 190 200
QQLDYGIYII HQTGSKKFNR AKLLNVGYLE ALKEQNWDCF IFHDVDLVPE
210 220 230 240 250
NDFNLYTCGD QPKHLVVGRN STGYRLRYSK YFGGVTALSR EQFFKVNGFS
260 270 280 290 300
NNYWGWGGED DDLRLRVELH KMKISRPNPD VGKYTMIFHT RDKGNEVNVD
310 320 330 340
RMKLLHQMSR VWKTDGLSSC SYRLLSVEHN PLYTNITVDF WTGV
Length:344
Mass (Da):39,893
Last modified:June 1, 2003 - v1
Checksum:i23AA84AB3187E53E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY117538 mRNA. Translation: AAM77197.1.
RefSeqiNP_001233624.1. NM_001246695.1.
XP_007647946.1. XM_007649756.2.
XP_007647947.1. XM_007649757.2.
XP_007647948.1. XM_007649758.2.
XP_016834525.1. XM_016979036.1.

Genome annotation databases

GeneIDi100689435.
KEGGicge:100689435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY117538 mRNA. Translation: AAM77197.1.
RefSeqiNP_001233624.1. NM_001246695.1.
XP_007647946.1. XM_007649756.2.
XP_007647947.1. XM_007649757.2.
XP_007647948.1. XM_007649758.2.
XP_016834525.1. XM_016979036.1.

3D structure databases

ProteinModelPortaliQ80WN7.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100689435.
KEGGicge:100689435.

Organism-specific databases

CTDi8702.

Phylogenomic databases

HOVERGENiHBG058334.
KOiK07969.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB4GT4_CRIGR
AccessioniPrimary (citable) accession number: Q80WN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.