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Q80WK2

- OSTB_MOUSE

UniProt

Q80WK2 - OSTB_MOUSE

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Protein

Organic solute transporter subunit beta

Gene

Slc51b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids. Modulates SLC51A glycosylation, membrane trafficking and stability activities.2 Publications

GO - Molecular functioni

  1. protein heterodimerization activity Source: UniProtKB
  2. transporter activity Source: MGI

GO - Biological processi

  1. bile acid and bile salt transport Source: UniProtKB
  2. positive regulation of protein exit from endoplasmic reticulum Source: UniProtKB
  3. positive regulation of protein glycosylation Source: UniProtKB
  4. positive regulation of protein targeting to membrane Source: UniProtKB
  5. regulation of protein stability Source: UniProtKB
  6. transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Organic solute transporter subunit beta
Short name:
OST-beta
Alternative name(s):
Solute carrier family 51 subunit beta
Gene namesi
Name:Slc51b
Synonyms:Ostb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:3582052. Slc51b.

Subcellular locationi

Cell membrane 3 Publications; Single-pass membrane protein 3 Publications
Note: Mainly restricted to the lateral and basal membranes of ileal enterocytes.

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
  2. plasma membrane Source: UniProtKB
  3. protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi29 – 302ED → AA: Does not inhibit transport activity. Does not inhibit colocalization with SLC51A at the plasma membrane. Does not decrease glycosylation of SLC51A. 1 Publication
Mutagenesisi34 – 352WN → AA: Inhibits transport activity. Inhibits weakly colocalization with SLC51A at the plasma membrane. Decreases glycosylation of SLC51A. 1 Publication
Mutagenesisi34 – 352WN → FQ: Does not inhibit transport activity. Does not inhibit colocalization with SLC51A at the plasma membrane. Does not decrease glycosylation of SLC51A. 1 Publication
Mutagenesisi54 – 552RR → AA: Inhibits transport activity. Inhibits localization at the plasma membrane and membrane orientation. Decreases glycosylation of SLC51A. 1 Publication
Mutagenesisi61 – 611R → G: Does not inhibit transport activity. Does not inhibit colocalization with SLC51A at the plasma membrane. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 128128Organic solute transporter subunit betaPRO_0000331555Add
BLAST

Proteomic databases

MaxQBiQ80WK2.
PaxDbiQ80WK2.
PRIDEiQ80WK2.

PTM databases

PhosphoSiteiQ80WK2.

Expressioni

Tissue specificityi

Present at high level in ileum. In ileum, it is restricted to the apical domain on the mature villus enterocytes with little detectable expression in the goblet cells or crypt enterocytes (at protein level). Expressed in kidney but not in heart, brain, liver, spleen, embryo, lung, thymus, ovary nor testis.1 Publication

Inductioni

Positively regulated via the bile acid-activated nuclear receptor farnesoid X receptor (NR1H4/FXR).2 Publications

Gene expression databases

BgeeiQ80WK2.
GenevestigatoriQ80WK2.

Interactioni

Subunit structurei

Interacts with SLC51A. The Ost-alpha/Ost-beta complex is a heterodimer composed of alpha (SLC51A) and beta (SLC51B) subunit; induces the transport of SLC51A from the reticulum endoplasmic to the plasma membrane.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ80WK2.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030ExtracellularSequence AnalysisAdd
BLAST
Topological domaini54 – 12875CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei31 – 5323HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domaini

The transmembrane domain (TM) is the major site of interaction with SLC51A. The extracellular-membrane interface is absolutely required for transport activity. The intracellular-membrane interface is necessary for establishing the correct membrane orientation that is essential for the heterodimer Ost-alpha/Ost-beta complex formation and transport activity at the cell membrane surface.

Sequence similaritiesi

Belongs to the OST-beta family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG44194.
GeneTreeiENSGT00390000010409.
HOGENOMiHOG000115298.
HOVERGENiHBG095150.
InParanoidiQ80WK2.
KOiK14361.
OMAiQANRNQK.
OrthoDBiEOG7XSTH6.
PhylomeDBiQ80WK2.
TreeFamiTF337010.

Family and domain databases

InterProiIPR029387. OSTbeta.
[Graphical view]
PfamiPF15048. OSTbeta. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80WK2 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDHSAEKAAA NAEVPQELLE EMLWYFRAED AAPWNYSILV LAVLVVMTSM
60 70 80 90 100
FLLRRSILAN RNRKKQPQDK ETPEDLHLDD SIMKENNSQV FLRETLISEK
110 120
PDLAPGEPEL KEKDSSLVFL PDPQETES
Length:128
Mass (Da):14,684
Last modified:June 1, 2003 - v1
Checksum:iADE8D76D128B68B9
GO

Sequence cautioni

The sequence BAE20418.1 differs from that shown. Reason: Erroneous termination at position 110. Translated as Leu.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701K → Q in AAI15607. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY279396 mRNA. Translation: AAP23994.1.
AK002463 mRNA. Translation: BAE20415.1.
AK004188 mRNA. Translation: BAE20418.1. Sequence problems.
BC115606 mRNA. Translation: AAI15607.1.
BC115607 mRNA. Translation: AAI15608.1.
CCDSiCCDS23291.1.
RefSeqiNP_849264.1. NM_178933.2.
UniGeneiMm.335247.

Genome annotation databases

EnsembliENSMUST00000065894; ENSMUSP00000064494; ENSMUSG00000053862.
GeneIDi330962.
KEGGimmu:330962.
UCSCiuc009qdg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY279396 mRNA. Translation: AAP23994.1 .
AK002463 mRNA. Translation: BAE20415.1 .
AK004188 mRNA. Translation: BAE20418.1 . Sequence problems.
BC115606 mRNA. Translation: AAI15607.1 .
BC115607 mRNA. Translation: AAI15608.1 .
CCDSi CCDS23291.1.
RefSeqi NP_849264.1. NM_178933.2.
UniGenei Mm.335247.

3D structure databases

ProteinModelPortali Q80WK2.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q80WK2.

Proteomic databases

MaxQBi Q80WK2.
PaxDbi Q80WK2.
PRIDEi Q80WK2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000065894 ; ENSMUSP00000064494 ; ENSMUSG00000053862 .
GeneIDi 330962.
KEGGi mmu:330962.
UCSCi uc009qdg.1. mouse.

Organism-specific databases

CTDi 123264.
MGIi MGI:3582052. Slc51b.

Phylogenomic databases

eggNOGi NOG44194.
GeneTreei ENSGT00390000010409.
HOGENOMi HOG000115298.
HOVERGENi HBG095150.
InParanoidi Q80WK2.
KOi K14361.
OMAi QANRNQK.
OrthoDBi EOG7XSTH6.
PhylomeDBi Q80WK2.
TreeFami TF337010.

Miscellaneous databases

NextBioi 399658.
PROi Q80WK2.
SOURCEi Search...

Gene expression databases

Bgeei Q80WK2.
Genevestigatori Q80WK2.

Family and domain databases

InterProi IPR029387. OSTbeta.
[Graphical view ]
Pfami PF15048. OSTbeta. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional complementation between a novel mammalian polygenic transport complex and an evolutionarily ancient organic solute transporter, OSTalpha-OSTbeta."
    Seward D.J., Koh A.S., Boyer J.L., Ballatori N.
    J. Biol. Chem. 278:27473-27482(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "The heteromeric organic solute transporter alpha-beta, Ostalpha-Ostbeta, is an ileal basolateral bile acid transporter."
    Dawson P.A., Hubbert M., Haywood J., Craddock A.L., Zerangue N., Christian W.V., Ballatori N.
    J. Biol. Chem. 280:6960-6968(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "Regulation of the mouse organic solute transporter alpha-beta, Ostalpha-Ostbeta, by bile acids."
    Frankenberg T., Rao A., Chen F., Haywood J., Shneider B.L., Dawson P.A.
    Am. J. Physiol. 290:G912-G922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY NR1H4.
  6. "Mrp4-/- mice have an impaired cytoprotective response in obstructive cholestasis."
    Mennone A., Soroka C.J., Cai S.Y., Harry K., Adachi M., Hagey L., Schuetz J.D., Boyer J.L.
    Hepatology 43:1013-1021(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Heterodimerization, trafficking and membrane topology of the two proteins, Ost alpha and Ost beta, that constitute the organic solute and steroid transporter."
    Li N., Cui Z., Fang F., Lee J.Y., Ballatori N.
    Biochem. J. 407:363-372(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF THE OST-ALPHA/OST-BETA COMPLEX, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH SLC51A.
  8. "beta-Subunit of the Ostalpha-Ostbeta organic solute transporter is required not only for heterodimerization and trafficking but also for function."
    Christian W.V., Li N., Hinkle P.M., Ballatori N.
    J. Biol. Chem. 287:21233-21243(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE OST-ALPHA/OST-BETA COMPLEX IN BILE TRANSPORT, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF 29-GLU-ASP-30; 34-TRP-ASN-35; 54-ARG-ARG-55 AND ARG-61.

Entry informationi

Entry nameiOSTB_MOUSE
AccessioniPrimary (citable) accession number: Q80WK2
Secondary accession number(s): Q14BU3, Q3V4C1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3