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Q80WK2 (OSTB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Organic solute transporter subunit beta

Short name=OST-beta
Alternative name(s):
Solute carrier family 51 subunit beta
Gene names
Name:Slc51b
Synonyms:Ostb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length128 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids. Modulates SLC51A glycosylation, membrane trafficking and stability activities. Ref.4 Ref.8

Subunit structure

Interacts with SLC51A. The Ost-alpha/Ost-beta complex is a heterodimer composed of alpha (SLC51A) and beta (SLC51B) subunit; induces the transport of SLC51A from the reticulum endoplasmic to the plasma membrane. Ref.7

Subcellular location

Cell membrane; Single-pass membrane protein. Note: Mainly restricted to the lateral and basal membranes of ileal enterocytes. Ref.4 Ref.7 Ref.8

Tissue specificity

Present at high level in ileum. In ileum, it is restricted to the apical domain on the mature villus enterocytes with little detectable expression in the goblet cells or crypt enterocytes (at protein level). Expressed in kidney but not in heart, brain, liver, spleen, embryo, lung, thymus, ovary nor testis. Ref.4

Induction

Positively regulated via the bile acid-activated nuclear receptor farnesoid X receptor (NR1H4/FXR). Ref.5 Ref.6

Domain

The transmembrane domain (TM) is the major site of interaction with SLC51A. The extracellular-membrane interface is absolutely required for transport activity. The intracellular-membrane interface is necessary for establishing the correct membrane orientation that is essential for the heterodimer Ost-alpha/Ost-beta complex formation and transport activity at the cell membrane surface.

Sequence similarities

Belongs to the OST-beta family.

Sequence caution

The sequence BAE20418.1 differs from that shown. Reason: Erroneous termination at position 110. Translated as Leu.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 128128Organic solute transporter subunit beta
PRO_0000331555

Regions

Topological domain1 – 3030Extracellular Potential
Transmembrane31 – 5323Helical; Potential
Topological domain54 – 12875Cytoplasmic Potential

Experimental info

Mutagenesis29 – 302ED → AA: Does not inhibit transport activity. Does not inhibit colocalization with SLC51A at the plasma membrane. Does not decrease glycosylation of SLC51A.
Mutagenesis34 – 352WN → AA: Inhibits transport activity. Inhibits weakly colocalization with SLC51A at the plasma membrane. Decreases glycosylation of SLC51A.
Mutagenesis34 – 352WN → FQ: Does not inhibit transport activity. Does not inhibit colocalization with SLC51A at the plasma membrane. Does not decrease glycosylation of SLC51A.
Mutagenesis54 – 552RR → AA: Inhibits transport activity. Inhibits localization at the plasma membrane and membrane orientation. Decreases glycosylation of SLC51A.
Mutagenesis611R → G: Does not inhibit transport activity. Does not inhibit colocalization with SLC51A at the plasma membrane. Ref.8
Sequence conflict701K → Q in AAI15607. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q80WK2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: ADE8D76D128B68B9

FASTA12814,684
        10         20         30         40         50         60 
MDHSAEKAAA NAEVPQELLE EMLWYFRAED AAPWNYSILV LAVLVVMTSM FLLRRSILAN 

        70         80         90        100        110        120 
RNRKKQPQDK ETPEDLHLDD SIMKENNSQV FLRETLISEK PDLAPGEPEL KEKDSSLVFL 


PDPQETES 

« Hide

References

« Hide 'large scale' references
[1]"Functional complementation between a novel mammalian polygenic transport complex and an evolutionarily ancient organic solute transporter, OSTalpha-OSTbeta."
Seward D.J., Koh A.S., Boyer J.L., Ballatori N.
J. Biol. Chem. 278:27473-27482(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The heteromeric organic solute transporter alpha-beta, Ostalpha-Ostbeta, is an ileal basolateral bile acid transporter."
Dawson P.A., Hubbert M., Haywood J., Craddock A.L., Zerangue N., Christian W.V., Ballatori N.
J. Biol. Chem. 280:6960-6968(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]"Regulation of the mouse organic solute transporter alpha-beta, Ostalpha-Ostbeta, by bile acids."
Frankenberg T., Rao A., Chen F., Haywood J., Shneider B.L., Dawson P.A.
Am. J. Physiol. 290:G912-G922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY NR1H4.
[6]"Mrp4-/- mice have an impaired cytoprotective response in obstructive cholestasis."
Mennone A., Soroka C.J., Cai S.Y., Harry K., Adachi M., Hagey L., Schuetz J.D., Boyer J.L.
Hepatology 43:1013-1021(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Heterodimerization, trafficking and membrane topology of the two proteins, Ost alpha and Ost beta, that constitute the organic solute and steroid transporter."
Li N., Cui Z., Fang F., Lee J.Y., Ballatori N.
Biochem. J. 407:363-372(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF THE OST-ALPHA/OST-BETA COMPLEX, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH SLC51A.
[8]"beta-Subunit of the Ostalpha-Ostbeta organic solute transporter is required not only for heterodimerization and trafficking but also for function."
Christian W.V., Li N., Hinkle P.M., Ballatori N.
J. Biol. Chem. 287:21233-21243(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE OST-ALPHA/OST-BETA COMPLEX IN BILE TRANSPORT, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF 29-GLU-ASP-30; 34-TRP-ASN-35; 54-ARG-ARG-55 AND ARG-61.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY279396 mRNA. Translation: AAP23994.1.
AK002463 mRNA. Translation: BAE20415.1.
AK004188 mRNA. Translation: BAE20418.1. Sequence problems.
BC115606 mRNA. Translation: AAI15607.1.
BC115607 mRNA. Translation: AAI15608.1.
RefSeqNP_849264.1. NM_178933.2.
UniGeneMm.335247.

3D structure databases

ProteinModelPortalQ80WK2.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ80WK2.

Proteomic databases

PaxDbQ80WK2.
PRIDEQ80WK2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000065894; ENSMUSP00000064494; ENSMUSG00000053862.
GeneID330962.
KEGGmmu:330962.
UCSCuc009qdg.1. mouse.

Organism-specific databases

CTD123264.
MGIMGI:3582052. Slc51b.

Phylogenomic databases

eggNOGNOG44194.
GeneTreeENSGT00390000010409.
HOGENOMHOG000115298.
HOVERGENHBG095150.
InParanoidQ80WK2.
KOK14361.
OMAEDATPWN.
OrthoDBEOG7XSTH6.
PhylomeDBQ80WK2.
TreeFamTF337010.

Gene expression databases

BgeeQ80WK2.
GenevestigatorQ80WK2.

Family and domain databases

ProtoNetSearch...

Other

NextBio399658.
PROQ80WK2.
SOURCESearch...

Entry information

Entry nameOSTB_MOUSE
AccessionPrimary (citable) accession number: Q80WK2
Secondary accession number(s): Q14BU3, Q3V4C1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot