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Protein

E3 ubiquitin-protein ligase Trim36

Gene

Trim36

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri33 – 84RING-type; degeneratePROSITE-ProRule annotationAdd BLAST52
Zinc fingeri154 – 192B box-type 1PROSITE-ProRule annotationAdd BLAST39
Zinc fingeri207 – 249B box-type 2PROSITE-ProRule annotationAdd BLAST43

GO - Molecular functioni

GO - Biological processi

  • acrosome reaction Source: MGI
  • microtubule bundle formation Source: MGI
  • regulation of cell cycle Source: InterPro

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Trim36 (EC:2.3.2.27)
Alternative name(s):
Acrosome RBCC protein
Haprin
RING-type E3 ubiquitin transferase TRIM36Curated
Tripartite motif-containing protein 36
Gene namesi
Name:Trim36
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:106264. Trim36.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: MGI
  • extracellular exosome Source: MGI
  • microtubule cytoskeleton Source: MGI

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562531 – 729E3 ubiquitin-protein ligase Trim36Add BLAST729

Proteomic databases

EPDiQ80WG7.
MaxQBiQ80WG7.
PaxDbiQ80WG7.
PRIDEiQ80WG7.

PTM databases

iPTMnetiQ80WG7.
PhosphoSitePlusiQ80WG7.

Expressioni

Tissue specificityi

Expressed only in testis.1 Publication

Developmental stagei

Expressed only in testicular germ cells after meiotic division. Expression was first detected at the age of 4 weeks.1 Publication

Gene expression databases

BgeeiENSMUSG00000033949.
CleanExiMM_TRIM36.
ExpressionAtlasiQ80WG7. baseline and differential.
GenevisibleiQ80WG7. MM.

Interactioni

Subunit structurei

Interacts with CENPH.1 Publication

Protein-protein interaction databases

IntActiQ80WG7. 1 interactor.
STRINGi10090.ENSMUSP00000037978.

Structurei

3D structure databases

ProteinModelPortaliQ80WG7.
SMRiQ80WG7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini356 – 413COSPROSITE-ProRule annotationAdd BLAST58
Domaini416 – 511Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST96
Domaini509 – 723B30.2/SPRYPROSITE-ProRule annotationAdd BLAST215

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili271 – 302Sequence analysisAdd BLAST32

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri33 – 84RING-type; degeneratePROSITE-ProRule annotationAdd BLAST52
Zinc fingeri154 – 192B box-type 1PROSITE-ProRule annotationAdd BLAST39
Zinc fingeri207 – 249B box-type 2PROSITE-ProRule annotationAdd BLAST43

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFI. Eukaryota.
ENOG410Z6W7. LUCA.
GeneTreeiENSGT00760000118878.
HOGENOMiHOG000049213.
HOVERGENiHBG059072.
InParanoidiQ80WG7.
KOiK12013.
OMAiKILMCPE.
OrthoDBiEOG091G020H.
PhylomeDBiQ80WG7.
TreeFamiTF315216.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.30.40.10. 2 hits.
InterProiView protein in InterPro
IPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR017903. COS_domain.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR027726. Trim36.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PANTHERiPTHR24103:SF510. PTHR24103:SF510. 1 hit.
PfamiView protein in Pfam
PF00041. fn3. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
SMARTiView protein in SMART
SM00336. BBOX. 1 hit.
SM00184. RING. 1 hit.
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS50188. B302_SPRY. 1 hit.
PS51262. COS. 1 hit.
PS50853. FN3. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q80WG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESEEISEF GYIMELLAKG KVTIKNIEKE LICPACKELF THPLILPCQH
60 70 80 90 100
SVCHKCVKEL LLSLDDSFND VASDSSNQSS PRLRLTSPSM DKIDKINRPG
110 120 130 140 150
WKRNSLTPRP TTFPCPGCEH DVDLGERGVS GLFRNFTLET IVERYRQAAR
160 170 180 190 200
AATAIMCDLC KPPPQESTKS CMDCSASYCN ECFKIYHPWG TVKAQHEYVG
210 220 230 240 250
PTTNFRPKVL MCPEHETERI NMYCELCRRP VCHLCKLGGN HSNHRVTTMS
260 270 280 290 300
SAYKTLKEKL SKDIDFLIGK ESQVKSQISE LNLLMKETEC NGERAKEEAL
310 320 330 340 350
AHFEKLFEIL EDRKSSVLKA IDASKKLRLD KFHTQMEEYQ GLLENNGLVG
360 370 380 390 400
YAQEVLKETD QSCFVQTAKQ LHLRIQKATE SLKSFRPAAQ ASFEDYVVNI
410 420 430 440 450
SKQTEVLGEL SFFSSGIDIP EINEEQSKVY NNALIDWHHP EKDKADSYVL
460 470 480 490 500
EYRKINRDEE MISWNEIEVH GTSKVVSNLE SNSPYAFRVR AYRGSICSPC
510 520 530 540 550
SRELILHTPP APVFSFLFDE KCGYNTEHLL LNLKRDRVES RAGFNVLLAA
560 570 580 590 600
ERIQVGHYTS LDYIIGDVGV TKGKHFWACR VEPYSYLVKV GVASSDKLQE
610 620 630 640 650
WLRSPRDAAS PRYEQDSGHD SGSEDACFDS SQPFTLVTIG MKKFFIPKSP
660 670 680 690 700
TSSNEPENRV LPMPTSIGIF LDCDKGKVSF YDMDHMKCLY ERQVDCSHTM
710 720
YPAFALMGSG GIQLEEAITA KYLEYEEDV
Length:729
Mass (Da):82,791
Last modified:December 7, 2004 - v2
Checksum:iE13CC617EFD47F58
GO

Sequence cautioni

The sequence BAC76066 differs from that shown. Reason: Frameshift at positions 356 and 369.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti292G → V in BAC76066 (PubMed:12917430).Curated1
Sequence conflicti532N → D in BAC76066 (PubMed:12917430).Curated1
Sequence conflicti601 – 602WL → CV in BAC76066 (PubMed:12917430).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB103063 mRNA. Translation: BAC76066.1. Frameshift.
AY251389 mRNA. Translation: AAP51209.1.
BC058106 mRNA. Translation: AAH58106.1.
CCDSiCCDS29229.1.
RefSeqiNP_001164326.1. NM_001170855.1.
NP_849203.2. NM_178872.4.
UniGeneiMm.277382.

Genome annotation databases

EnsembliENSMUST00000037011; ENSMUSP00000037978; ENSMUSG00000033949.
GeneIDi28105.
KEGGimmu:28105.
UCSCiuc008evi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB103063 mRNA. Translation: BAC76066.1. Frameshift.
AY251389 mRNA. Translation: AAP51209.1.
BC058106 mRNA. Translation: AAH58106.1.
CCDSiCCDS29229.1.
RefSeqiNP_001164326.1. NM_001170855.1.
NP_849203.2. NM_178872.4.
UniGeneiMm.277382.

3D structure databases

ProteinModelPortaliQ80WG7.
SMRiQ80WG7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80WG7. 1 interactor.
STRINGi10090.ENSMUSP00000037978.

PTM databases

iPTMnetiQ80WG7.
PhosphoSitePlusiQ80WG7.

Proteomic databases

EPDiQ80WG7.
MaxQBiQ80WG7.
PaxDbiQ80WG7.
PRIDEiQ80WG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037011; ENSMUSP00000037978; ENSMUSG00000033949.
GeneIDi28105.
KEGGimmu:28105.
UCSCiuc008evi.2. mouse.

Organism-specific databases

CTDi55521.
MGIiMGI:106264. Trim36.

Phylogenomic databases

eggNOGiENOG410ITFI. Eukaryota.
ENOG410Z6W7. LUCA.
GeneTreeiENSGT00760000118878.
HOGENOMiHOG000049213.
HOVERGENiHBG059072.
InParanoidiQ80WG7.
KOiK12013.
OMAiKILMCPE.
OrthoDBiEOG091G020H.
PhylomeDBiQ80WG7.
TreeFamiTF315216.

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiPR:Q80WG7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033949.
CleanExiMM_TRIM36.
ExpressionAtlasiQ80WG7. baseline and differential.
GenevisibleiQ80WG7. MM.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.30.40.10. 2 hits.
InterProiView protein in InterPro
IPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR017903. COS_domain.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR027726. Trim36.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PANTHERiPTHR24103:SF510. PTHR24103:SF510. 1 hit.
PfamiView protein in Pfam
PF00041. fn3. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
SMARTiView protein in SMART
SM00336. BBOX. 1 hit.
SM00184. RING. 1 hit.
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS50188. B302_SPRY. 1 hit.
PS51262. COS. 1 hit.
PS50853. FN3. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRI36_MOUSE
AccessioniPrimary (citable) accession number: Q80WG7
Secondary accession number(s): Q7TNM1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: April 12, 2017
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.