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Protein

Fragile X mental retardation protein 1 homolog

Gene

Fmr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression. RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. Involved in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C) (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fragile X mental retardation protein 1 homolog
Short name:
FMRP
Short name:
Protein FMR-1
Gene namesi
Name:Fmr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi2623. Fmr1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Nucleusnucleolus By similarity

GO - Cellular componenti

  • axon Source: RGD
  • axon terminus Source: UniProtKB
  • cell body Source: MGI
  • dendrite Source: UniProtKB
  • dendritic shaft Source: RGD
  • dendritic spine Source: UniProtKB
  • mRNA cap binding complex Source: UniProtKB
  • neuronal ribonucleoprotein granule Source: MGI
  • neuron projection Source: RGD
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • perikaryon Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • polysome Source: UniProtKB
  • postsynaptic density Source: UniProtKB
  • synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Fragile X mental retardation protein 1 homologPRO_0000342185Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei336 – 3361PhosphoserineCombined sources
Modified residuei337 – 3371PhosphoserineCombined sources
Modified residuei347 – 3471PhosphoserineCombined sources
Modified residuei349 – 3491PhosphoserineCombined sources
Modified residuei350 – 3501PhosphoserineCombined sources
Modified residuei369 – 3691PhosphoserineBy similarity
Modified residuei441 – 4411PhosphothreonineBy similarity
Modified residuei478 – 4781PhosphoserineBy similarity
Modified residuei522 – 5221Omega-N-methylarginine; alternateBy similarity
Modified residuei522 – 5221Omega-N-methylated arginine; alternateBy similarity
Modified residuei581 – 5811PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on several serine residues.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ80WE1.
PRIDEiQ80WE1.

PTM databases

iPTMnetiQ80WE1.
PhosphoSiteiQ80WE1.

Expressioni

Gene expression databases

ExpressionAtlasiQ80WE1. differential.

Interactioni

Subunit structurei

Homooligomer. Found in a RNP granule complex with IGF2BP1. Directly interacts with SMN and TDRD3. Interacts with the SMN core complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts with FXR1, FXR2, IGF2BP1, NUFIP1, NUFIP2, MCRS1 and RANBP9. Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with CYFIP1 and CYFIP2. The interaction with brain cytoplasmic RNA 1 (BC1) increases binding affinity for the CYFIP1-EIF4E complex in the brain (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247050. 1 interaction.
IntActiQ80WE1. 1 interaction.
STRINGi10116.ENSRNOP00000016227.

Structurei

3D structure databases

ProteinModelPortaliQ80WE1.
SMRiQ80WE1. Positions 1-134, 216-403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 5047Agenet-like 1Add
BLAST
Domaini63 – 11553Agenet-like 2Add
BLAST
Domaini218 – 27962KH 1PROSITE-ProRule annotationAdd
BLAST
Domaini281 – 34868KH 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni397 – 593197Interaction with RANBP9By similarityAdd
BLAST
Regioni512 – 52615RNA-binding RGG-boxBy similarityAdd
BLAST

Domaini

The tandem Tudor domains preferentially recognize trimethylated histone peptides.By similarity

Sequence similaritiesi

Belongs to the FMR1 family.Curated
Contains 2 Agenet-like domains.Curated
Contains 2 KH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF9J. Eukaryota.
ENOG410ZDJG. LUCA.
GeneTreeiENSGT00390000017033.
HOGENOMiHOG000293377.
HOVERGENiHBG005739.
InParanoidiQ80WE1.
KOiK15516.
OMAiDNVDGQQ.
OrthoDBiEOG7NKKJT.
PhylomeDBiQ80WE1.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR008395. Agenet-like_dom.
IPR032196. FXMR_C2.
IPR022034. FXMRP1_C_core.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF05641. Agenet. 1 hit.
PF16098. FXMR_C2. 2 hits.
PF12235. FXMRP1_C_core. 1 hit.
PF00013. KH_1. 2 hits.
[Graphical view]
SMARTiSM00322. KH. 2 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 2 hits.
PROSITEiPS51641. AGENET_LIKE. 2 hits.
PS50084. KH_TYPE_1. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80WE1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEELVVEVRG SNGAFYKAFV KDVHEDSITV AFENNWQPER QIPFHDVRFP
60 70 80 90 100
PPVGYNKDIN ESDEVEVYSR ANEKEPCCWW LAKVRMIKGE FYVIEYAACD
110 120 130 140 150
ATYNEIVTIE RLRSVNPNKP ATKDTFHKIK LEVPEDLRQM CAKESAHKDF
160 170 180 190 200
KKAVGAFSVT YDPENYQLVI LSINEVTSKR AHMLIDMHFR SLRTKLSLIL
210 220 230 240 250
RNEEASKQLE SSRQLASRFH EQFIVREDLM GLAIGTHGAN IQQARKVPGV
260 270 280 290 300
TAIDLDEDTC TFHIYGEDQD AVKKARSFLE FAEDVIQVPR NLVGKVIGKN
310 320 330 340 350
GKLIQEIVDK SGVVRVRIEA ENEKSVPQEE ENLPPSSLPS NNSRVGSNSS
360 370 380 390 400
EEKKHLDTKE NTHFSQPNST KVQRGMVPFV FVGTKDSIAN ATVLLDYHLN
410 420 430 440 450
YLKEVDQLRL ERLQIDEQLR QIGASSRPPP NRTDKEKGYV TDDGQGMGRG
460 470 480 490 500
SRPYRNRGHG RRGPGYTSGT NSEASNASET ESDHRDELSD WSLAPTEEER
510 520 530 540 550
ESFLRRGDGR RRGGGGRGQG GRGRGGGFKG NDDHSRTDNR PRNPRETKGR
560 570 580 590
TTDGSLQSTS SEGSRLRTGK DRNQKKEKPD SVDGLQPLVN GVP
Length:593
Mass (Da):66,780
Last modified:July 1, 2008 - v2
Checksum:i8EFBEC47E6B818F4
GO
Isoform 2 (identifier: Q80WE1-2) [UniParc]FASTAAdd to basket

Also known as: 18

The sequence of this isoform differs from the canonical sequence as follows:
     469-480: Missing.

Show »
Length:581
Mass (Da):65,631
Checksum:i3963E9DB452EAB1D
GO

Sequence cautioni

The sequence AAB07073.1 differs from that shown.N-ter sequencing errors.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101E → D in AAB07073 (Ref. 2) Curated
Sequence conflicti184 – 1841L → S in AAB07073 (Ref. 2) Curated
Sequence conflicti424 – 4241A → V in AAB07073 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei469 – 48012Missing in isoform 2. 1 PublicationVSP_034393Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY240947 mRNA. Translation: AAP15341.1.
U60145 mRNA. Translation: AAB07073.1. Sequence problems.
RefSeqiNP_434691.1. NM_052804.1. [Q80WE1-2]
XP_006229594.1. XM_006229532.2. [Q80WE1-1]
UniGeneiRn.40595.

Genome annotation databases

EnsembliENSRNOT00000087893; ENSRNOP00000074033; ENSRNOG00000057464. [Q80WE1-2]
GeneIDi24948.
KEGGirno:24948.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY240947 mRNA. Translation: AAP15341.1.
U60145 mRNA. Translation: AAB07073.1. Sequence problems.
RefSeqiNP_434691.1. NM_052804.1. [Q80WE1-2]
XP_006229594.1. XM_006229532.2. [Q80WE1-1]
UniGeneiRn.40595.

3D structure databases

ProteinModelPortaliQ80WE1.
SMRiQ80WE1. Positions 1-134, 216-403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247050. 1 interaction.
IntActiQ80WE1. 1 interaction.
STRINGi10116.ENSRNOP00000016227.

PTM databases

iPTMnetiQ80WE1.
PhosphoSiteiQ80WE1.

Proteomic databases

PaxDbiQ80WE1.
PRIDEiQ80WE1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000087893; ENSRNOP00000074033; ENSRNOG00000057464. [Q80WE1-2]
GeneIDi24948.
KEGGirno:24948.

Organism-specific databases

CTDi2332.
RGDi2623. Fmr1.

Phylogenomic databases

eggNOGiENOG410IF9J. Eukaryota.
ENOG410ZDJG. LUCA.
GeneTreeiENSGT00390000017033.
HOGENOMiHOG000293377.
HOVERGENiHBG005739.
InParanoidiQ80WE1.
KOiK15516.
OMAiDNVDGQQ.
OrthoDBiEOG7NKKJT.
PhylomeDBiQ80WE1.

Miscellaneous databases

NextBioi604957.
PROiQ80WE1.

Gene expression databases

ExpressionAtlasiQ80WE1. differential.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR008395. Agenet-like_dom.
IPR032196. FXMR_C2.
IPR022034. FXMRP1_C_core.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF05641. Agenet. 1 hit.
PF16098. FXMR_C2. 2 hits.
PF12235. FXMRP1_C_core. 1 hit.
PF00013. KH_1. 2 hits.
[Graphical view]
SMARTiSM00322. KH. 2 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 2 hits.
PROSITEiPS51641. AGENET_LIKE. 2 hits.
PS50084. KH_TYPE_1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Messenger RNA encoding FMRP isoform 18 from the rat hippocampus."
    Rackham O., Brown C.M.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Sprague-Dawley.
  2. "Cloning of rat FMR1 gene."
    Huang T., Ji H., Sittler A., Shen Y., Mandel J., Wu G.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-587 (ISOFORM 1).
    Strain: Wistar.
    Tissue: Brain.
  3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-336; SER-337; SER-347; SER-349 AND SER-350, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFMR1_RAT
AccessioniPrimary (citable) accession number: Q80WE1
Secondary accession number(s): P70568
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: May 11, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

RNA-binding activity is inhibited by RANBP9.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.