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Q80WC9

- ACSF4_MOUSE

UniProt

Q80WC9 - ACSF4_MOUSE

Protein

Acyl-CoA synthetase family member 4

Gene

Aasdh

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA By similarity. Putative 2-aminoadipic 6-semialdehyde dehydrogenase, which may be involved in lysine catabolism.By similarity

    Catalytic activityi

    (S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei427 – 4271ATPBy similarity
    Binding sitei441 – 4411ATPBy similarity
    Binding sitei526 – 5261ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi197 – 2059ATPBy similarity

    GO - Molecular functioni

    1. acid-thiol ligase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. L-aminoadipate-semialdehyde dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fatty acid metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Ligase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, NAD, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-CoA synthetase family member 4 (EC:6.2.1.-)
    Alternative name(s):
    2-aminoadipic 6-semialdehyde dehydrogenase
    Protein LYS2 homolog
    Putative aminoadipate-semialdehyde dehydrogenase (EC:1.2.1.31)
    Gene namesi
    Name:Aasdh
    Synonyms:Acsf4, U26
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:2442517. Aasdh.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11001100Acyl-CoA synthetase family member 4PRO_0000315804Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei591 – 5911O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation
    Modified residuei651 – 6511PhosphoserineBy similarity

    Keywords - PTMi

    Phosphopantetheine, Phosphoprotein

    Proteomic databases

    PRIDEiQ80WC9.

    PTM databases

    PhosphoSiteiQ80WC9.

    Expressioni

    Inductioni

    According to PubMed:12712191, it is up-regulated by lysine-rich diet, while according to PubMed:17029795 levels of expression are not significantly changed even when diets differed markedly in PQQ and lysine content.2 Publications

    Gene expression databases

    BgeeiQ80WC9.
    CleanExiMM_AASDH.
    GenevestigatoriQ80WC9.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000113792.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80WC9.
    SMRiQ80WC9. Positions 150-606, 754-1091.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini554 – 62875Acyl carrierPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 acyl carrier domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1520.
    GeneTreeiENSGT00440000033811.
    HOGENOMiHOG000033793.
    HOVERGENiHBG057704.
    InParanoidiQ80WC9.
    KOiK00142.
    OMAiTMRATGD.
    OrthoDBiEOG77T14J.
    PhylomeDBiQ80WC9.
    TreeFamiTF314245.

    Family and domain databases

    Gene3Di1.10.1200.10. 1 hit.
    2.140.10.10. 1 hit.
    InterProiIPR009081. Acyl_carrier_prot-like.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    IPR006162. PPantetheine_attach_site.
    IPR018391. PQQ_beta_propeller_repeat.
    IPR027295. Quinonprotein_ADH-like_fam.
    IPR011047. Quinonprotein_ADH-like_supfam.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF00550. PP-binding. 1 hit.
    [Graphical view]
    SMARTiSM00564. PQQ. 6 hits.
    [Graphical view]
    SUPFAMiSSF47336. SSF47336. 1 hit.
    SSF50998. SSF50998. 1 hit.
    PROSITEiPS50075. ACP_DOMAIN. 1 hit.
    PS00455. AMP_BINDING. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q80WC9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTLQELVLRT ASVYMDRTAV CFDEGNNQPP VCYSYKALLS AASELSHFLI     50
    AHCDFGGIRE IGLYCQPGIN LPSWILGILQ VPAAYAPIDP DSPPSLSTYF 100
    MKKCDLKYVL VEKQQLSKFK SSHETVLNYD TVSVEHKDLA LFRLHWEDGR 150
    VSTVLGDRAD QHKVTDREDR VSAESRTPEK EHMDMRHDGC LAYVLHTSGT 200
    TGTPKIVRVP HACILPNIQH FRSLFDITQE DILFLASPLT FDPSVVEIFV 250
    SLSSGACLLI VPTSVKVLPS KLADILFSRH RVTVLQATPT LLRRFGSELI 300
    KSTVLSAHTS LRVLALGGEA FPSLTILKSW RGKGNRTQIF NIYGITEVSS 350
    WATFYRIPEE ILNSAVKHES PVQLGSPLLG TVIEVRDQNG SPVLEGTGQV 400
    FLGGKNRVCF LDDEMTVPLG TMRATGDFVT VKDGEIFFLG RKDSQIKRHG 450
    KRLNIALVQQ VAEELRQVES CAVTWYNQER LILFIVSKVD LVKDCIFKEL 500
    QKHLPAHALP DDMVLIDTLP FTCHGKVDVS ELNKIYLDYI SSQPRNELHG 550
    KEELWGKLQY LWKSILCLPE DPEDTLKVPA NSVFLDSGGD SLKSMRLLSE 600
    IERLTGTAIP GLLEVILSSS LLDVYNHIVQ AVFTPEDRKA NRSYTTKRKF 650
    SDADPEEASG KPARLESAWP SNHAGETNSV IALSRGSQVL SLGAGRLLTQ 700
    LGLCLPVCSL DLIPQTNTQI LKSLSPPAPD ENLEKPPLFQ QGSPVVGAMA 750
    MALRERWRSD TGKCVDASPL LVRAAVQDKP STTVYIGSHS HTVKAVDLSS 800
    GETRWEQLLG DRIESSACVS KCGNFIVVGC YNGLVYVLKS NSGEKYWTFT 850
    TEDAVKSSPA VDPTTGLIYV GSHDQHAYAL DIYEKKCVWK LNCEGALFSS 900
    PCVSLSPHHL YCATLGGLLL ALNPASGSTV WKRSCGKPLF SSPRCYQQYI 950
    CIGCVDGSLL CFTHSGEQVW RFAAGGPIFS SPCVSAAEQE IFFGSHDCFI 1000
    YCCSKEGHLR WKFETTARVY ATPFAFSNHP RSDDALLAAA STDGKLWVLE 1050
    SRSGELRSVY ELPGEVFSSP VVWESMLVIG CRNNYIYCLD LLCGDKNNQV 1100
    Length:1,100
    Mass (Da):121,569
    Last modified:June 1, 2003 - v1
    Checksum:i86B303CFF07B234C
    GO
    Isoform 2 (identifier: Q80WC9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         368-1100: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:367
    Mass (Da):40,964
    Checksum:i19368BB77FDDF580
    GO
    Isoform 3 (identifier: Q80WC9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         223-1100: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:222
    Mass (Da):25,031
    Checksum:i2850F469893CD77F
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei223 – 1100878Missing in isoform 3. 1 PublicationVSP_030714Add
    BLAST
    Alternative sequencei368 – 1100733Missing in isoform 2. 1 PublicationVSP_030715Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB095954 mRNA. Translation: BAC75954.1.
    AK038779 mRNA. Translation: BAE43303.1.
    AK043807 mRNA. Translation: BAC31660.1.
    BC094507 mRNA. Translation: AAH94507.1.
    BC128330 mRNA. Translation: AAI28331.1.
    CCDSiCCDS19366.1. [Q80WC9-1]
    RefSeqiNP_776126.1. NM_173765.3. [Q80WC9-1]
    XP_006534916.1. XM_006534853.1. [Q80WC9-1]
    UniGeneiMm.39271.

    Genome annotation databases

    EnsembliENSMUST00000069709; ENSMUSP00000069279; ENSMUSG00000055923. [Q80WC9-1]
    ENSMUST00000120963; ENSMUSP00000113792; ENSMUSG00000055923. [Q80WC9-1]
    ENSMUST00000146570; ENSMUSP00000117639; ENSMUSG00000055923.
    GeneIDi231326.
    KEGGimmu:231326.
    UCSCiuc008xvi.2. mouse. [Q80WC9-1]
    uc008xvk.2. mouse. [Q80WC9-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB095954 mRNA. Translation: BAC75954.1 .
    AK038779 mRNA. Translation: BAE43303.1 .
    AK043807 mRNA. Translation: BAC31660.1 .
    BC094507 mRNA. Translation: AAH94507.1 .
    BC128330 mRNA. Translation: AAI28331.1 .
    CCDSi CCDS19366.1. [Q80WC9-1 ]
    RefSeqi NP_776126.1. NM_173765.3. [Q80WC9-1 ]
    XP_006534916.1. XM_006534853.1. [Q80WC9-1 ]
    UniGenei Mm.39271.

    3D structure databases

    ProteinModelPortali Q80WC9.
    SMRi Q80WC9. Positions 150-606, 754-1091.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000113792.

    PTM databases

    PhosphoSitei Q80WC9.

    Proteomic databases

    PRIDEi Q80WC9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000069709 ; ENSMUSP00000069279 ; ENSMUSG00000055923 . [Q80WC9-1 ]
    ENSMUST00000120963 ; ENSMUSP00000113792 ; ENSMUSG00000055923 . [Q80WC9-1 ]
    ENSMUST00000146570 ; ENSMUSP00000117639 ; ENSMUSG00000055923 .
    GeneIDi 231326.
    KEGGi mmu:231326.
    UCSCi uc008xvi.2. mouse. [Q80WC9-1 ]
    uc008xvk.2. mouse. [Q80WC9-3 ]

    Organism-specific databases

    CTDi 132949.
    MGIi MGI:2442517. Aasdh.

    Phylogenomic databases

    eggNOGi COG1520.
    GeneTreei ENSGT00440000033811.
    HOGENOMi HOG000033793.
    HOVERGENi HBG057704.
    InParanoidi Q80WC9.
    KOi K00142.
    OMAi TMRATGD.
    OrthoDBi EOG77T14J.
    PhylomeDBi Q80WC9.
    TreeFami TF314245.

    Miscellaneous databases

    ChiTaRSi AASDH. mouse.
    NextBioi 380501.
    PROi Q80WC9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q80WC9.
    CleanExi MM_AASDH.
    Genevestigatori Q80WC9.

    Family and domain databases

    Gene3Di 1.10.1200.10. 1 hit.
    2.140.10.10. 1 hit.
    InterProi IPR009081. Acyl_carrier_prot-like.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    IPR006162. PPantetheine_attach_site.
    IPR018391. PQQ_beta_propeller_repeat.
    IPR027295. Quinonprotein_ADH-like_fam.
    IPR011047. Quinonprotein_ADH-like_supfam.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF00550. PP-binding. 1 hit.
    [Graphical view ]
    SMARTi SM00564. PQQ. 6 hits.
    [Graphical view ]
    SUPFAMi SSF47336. SSF47336. 1 hit.
    SSF50998. SSF50998. 1 hit.
    PROSITEi PS50075. ACP_DOMAIN. 1 hit.
    PS00455. AMP_BINDING. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nutritional biochemistry: a new redox-cofactor vitamin for mammals."
      Kasahara T., Kato T.
      Nature 422:832-832(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), POSSIBLE FUNCTION, INDUCTION.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-645 (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Brain cortex and Hypothalamus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Olfactory epithelium.
    4. "Biochemistry: role of PQQ as a mammalian enzyme cofactor?"
      Felton L.M., Anthony C.
      Nature 433:E10-E10(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMMENT ON PUBMED:12712191 RESULTS.
    5. "Biochemistry: is pyrroloquinoline quinone a vitamin?"
      Rucker R., Storms D., Sheets A., Tchaparian E., Fascetti A.
      Nature 433:E10-E11(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMMENT ON PUBMED:12712191 RESULTS.
    6. "Pyrroloquinoline quinone nutritional status alters lysine metabolism and modulates mitochondrial DNA content in the mouse and rat."
      Bauerly K.A., Storms D.H., Harris C.B., Hajizadeh S., Sun M.Y., Cheung C.P., Satre M.A., Fascetti A.J., Tchaparian E., Rucker R.B.
      Biochim. Biophys. Acta 1760:1741-1748(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiACSF4_MOUSE
    AccessioniPrimary (citable) accession number: Q80WC9
    Secondary accession number(s): Q3V3L2, Q505K4, Q8BRP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3