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Protein

Acyl-CoA synthetase family member 4

Gene

Aasdh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA (By similarity). Putative 2-aminoadipic 6-semialdehyde dehydrogenase, which may be involved in lysine catabolism.By similarity

Catalytic activityi

(S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei427ATPBy similarity1
Binding sitei441ATPBy similarity1
Binding sitei526ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 205ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

  • amino acid activation for nonribosomal peptide biosynthetic process Source: MGI
  • beta-alanine metabolic process Source: MGI
  • fatty acid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-CoA synthetase family member 4 (EC:6.2.1.-)
Alternative name(s):
2-aminoadipic 6-semialdehyde dehydrogenase
Protein LYS2 homolog
Putative aminoadipate-semialdehyde dehydrogenase (EC:1.2.1.31)
Gene namesi
Name:Aasdh
Synonyms:Acsf4, U26
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2442517. Aasdh.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003158041 – 1100Acyl-CoA synthetase family member 4Add BLAST1100

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei591O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1
Modified residuei651PhosphoserineBy similarity1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

PaxDbiQ80WC9.
PRIDEiQ80WC9.

PTM databases

iPTMnetiQ80WC9.
PhosphoSitePlusiQ80WC9.

Expressioni

Inductioni

According to PubMed:12712191, it is up-regulated by lysine-rich diet, while according to PubMed:17029795 levels of expression are not significantly changed even when diets differed markedly in PQQ and lysine content.2 Publications

Gene expression databases

BgeeiENSMUSG00000055923.
CleanExiMM_AASDH.
ExpressionAtlasiQ80WC9. baseline and differential.
GenevisibleiQ80WC9. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069279.

Structurei

3D structure databases

ProteinModelPortaliQ80WC9.
SMRiQ80WC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini554 – 628Acyl carrierPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Contains 1 acyl carrier domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1178. Eukaryota.
KOG4649. Eukaryota.
COG1520. LUCA.
GeneTreeiENSGT00440000033811.
HOGENOMiHOG000033793.
HOVERGENiHBG057704.
InParanoidiQ80WC9.
KOiK00142.
OMAiKECIPLA.
OrthoDBiEOG091G05QW.
PhylomeDBiQ80WC9.
TreeFamiTF314245.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
2.140.10.10. 1 hit.
InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR009081. PP-bd_ACP.
IPR006162. Ppantetheine_attach_site.
IPR025666. PQQ-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR002372. PQQ_repeat.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF00550. PP-binding. 1 hit.
PF13360. PQQ_2. 1 hit.
PF13570. PQQ_3. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 6 hits.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00455. AMP_BINDING. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80WC9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLQELVLRT ASVYMDRTAV CFDEGNNQPP VCYSYKALLS AASELSHFLI
60 70 80 90 100
AHCDFGGIRE IGLYCQPGIN LPSWILGILQ VPAAYAPIDP DSPPSLSTYF
110 120 130 140 150
MKKCDLKYVL VEKQQLSKFK SSHETVLNYD TVSVEHKDLA LFRLHWEDGR
160 170 180 190 200
VSTVLGDRAD QHKVTDREDR VSAESRTPEK EHMDMRHDGC LAYVLHTSGT
210 220 230 240 250
TGTPKIVRVP HACILPNIQH FRSLFDITQE DILFLASPLT FDPSVVEIFV
260 270 280 290 300
SLSSGACLLI VPTSVKVLPS KLADILFSRH RVTVLQATPT LLRRFGSELI
310 320 330 340 350
KSTVLSAHTS LRVLALGGEA FPSLTILKSW RGKGNRTQIF NIYGITEVSS
360 370 380 390 400
WATFYRIPEE ILNSAVKHES PVQLGSPLLG TVIEVRDQNG SPVLEGTGQV
410 420 430 440 450
FLGGKNRVCF LDDEMTVPLG TMRATGDFVT VKDGEIFFLG RKDSQIKRHG
460 470 480 490 500
KRLNIALVQQ VAEELRQVES CAVTWYNQER LILFIVSKVD LVKDCIFKEL
510 520 530 540 550
QKHLPAHALP DDMVLIDTLP FTCHGKVDVS ELNKIYLDYI SSQPRNELHG
560 570 580 590 600
KEELWGKLQY LWKSILCLPE DPEDTLKVPA NSVFLDSGGD SLKSMRLLSE
610 620 630 640 650
IERLTGTAIP GLLEVILSSS LLDVYNHIVQ AVFTPEDRKA NRSYTTKRKF
660 670 680 690 700
SDADPEEASG KPARLESAWP SNHAGETNSV IALSRGSQVL SLGAGRLLTQ
710 720 730 740 750
LGLCLPVCSL DLIPQTNTQI LKSLSPPAPD ENLEKPPLFQ QGSPVVGAMA
760 770 780 790 800
MALRERWRSD TGKCVDASPL LVRAAVQDKP STTVYIGSHS HTVKAVDLSS
810 820 830 840 850
GETRWEQLLG DRIESSACVS KCGNFIVVGC YNGLVYVLKS NSGEKYWTFT
860 870 880 890 900
TEDAVKSSPA VDPTTGLIYV GSHDQHAYAL DIYEKKCVWK LNCEGALFSS
910 920 930 940 950
PCVSLSPHHL YCATLGGLLL ALNPASGSTV WKRSCGKPLF SSPRCYQQYI
960 970 980 990 1000
CIGCVDGSLL CFTHSGEQVW RFAAGGPIFS SPCVSAAEQE IFFGSHDCFI
1010 1020 1030 1040 1050
YCCSKEGHLR WKFETTARVY ATPFAFSNHP RSDDALLAAA STDGKLWVLE
1060 1070 1080 1090 1100
SRSGELRSVY ELPGEVFSSP VVWESMLVIG CRNNYIYCLD LLCGDKNNQV
Length:1,100
Mass (Da):121,569
Last modified:June 1, 2003 - v1
Checksum:i86B303CFF07B234C
GO
Isoform 2 (identifier: Q80WC9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     368-1100: Missing.

Note: No experimental confirmation available.
Show »
Length:367
Mass (Da):40,964
Checksum:i19368BB77FDDF580
GO
Isoform 3 (identifier: Q80WC9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-1100: Missing.

Note: No experimental confirmation available.
Show »
Length:222
Mass (Da):25,031
Checksum:i2850F469893CD77F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030714223 – 1100Missing in isoform 3. 1 PublicationAdd BLAST878
Alternative sequenceiVSP_030715368 – 1100Missing in isoform 2. 1 PublicationAdd BLAST733

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095954 mRNA. Translation: BAC75954.1.
AK038779 mRNA. Translation: BAE43303.1.
AK043807 mRNA. Translation: BAC31660.1.
BC094507 mRNA. Translation: AAH94507.1.
BC128330 mRNA. Translation: AAI28331.1.
CCDSiCCDS19366.1. [Q80WC9-1]
RefSeqiNP_776126.1. NM_173765.3. [Q80WC9-1]
XP_006534916.1. XM_006534853.2. [Q80WC9-1]
XP_011247723.1. XM_011249421.1. [Q80WC9-1]
XP_011247724.1. XM_011249422.1. [Q80WC9-1]
UniGeneiMm.39271.

Genome annotation databases

EnsembliENSMUST00000069709; ENSMUSP00000069279; ENSMUSG00000055923. [Q80WC9-1]
ENSMUST00000120963; ENSMUSP00000113792; ENSMUSG00000055923. [Q80WC9-1]
GeneIDi231326.
KEGGimmu:231326.
UCSCiuc008xvi.2. mouse. [Q80WC9-1]
uc008xvk.2. mouse. [Q80WC9-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095954 mRNA. Translation: BAC75954.1.
AK038779 mRNA. Translation: BAE43303.1.
AK043807 mRNA. Translation: BAC31660.1.
BC094507 mRNA. Translation: AAH94507.1.
BC128330 mRNA. Translation: AAI28331.1.
CCDSiCCDS19366.1. [Q80WC9-1]
RefSeqiNP_776126.1. NM_173765.3. [Q80WC9-1]
XP_006534916.1. XM_006534853.2. [Q80WC9-1]
XP_011247723.1. XM_011249421.1. [Q80WC9-1]
XP_011247724.1. XM_011249422.1. [Q80WC9-1]
UniGeneiMm.39271.

3D structure databases

ProteinModelPortaliQ80WC9.
SMRiQ80WC9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069279.

PTM databases

iPTMnetiQ80WC9.
PhosphoSitePlusiQ80WC9.

Proteomic databases

PaxDbiQ80WC9.
PRIDEiQ80WC9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069709; ENSMUSP00000069279; ENSMUSG00000055923. [Q80WC9-1]
ENSMUST00000120963; ENSMUSP00000113792; ENSMUSG00000055923. [Q80WC9-1]
GeneIDi231326.
KEGGimmu:231326.
UCSCiuc008xvi.2. mouse. [Q80WC9-1]
uc008xvk.2. mouse. [Q80WC9-3]

Organism-specific databases

CTDi132949.
MGIiMGI:2442517. Aasdh.

Phylogenomic databases

eggNOGiKOG1178. Eukaryota.
KOG4649. Eukaryota.
COG1520. LUCA.
GeneTreeiENSGT00440000033811.
HOGENOMiHOG000033793.
HOVERGENiHBG057704.
InParanoidiQ80WC9.
KOiK00142.
OMAiKECIPLA.
OrthoDBiEOG091G05QW.
PhylomeDBiQ80WC9.
TreeFamiTF314245.

Miscellaneous databases

ChiTaRSiAasdh. mouse.
PROiQ80WC9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000055923.
CleanExiMM_AASDH.
ExpressionAtlasiQ80WC9. baseline and differential.
GenevisibleiQ80WC9. MM.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
2.140.10.10. 1 hit.
InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR009081. PP-bd_ACP.
IPR006162. Ppantetheine_attach_site.
IPR025666. PQQ-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR002372. PQQ_repeat.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF00550. PP-binding. 1 hit.
PF13360. PQQ_2. 1 hit.
PF13570. PQQ_3. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 6 hits.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00455. AMP_BINDING. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACSF4_MOUSE
AccessioniPrimary (citable) accession number: Q80WC9
Secondary accession number(s): Q3V3L2, Q505K4, Q8BRP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

According to PubMed:12712191, it may bind pyrroloquinoline quinone (PQQ) cofactor. However, their conclusions are based on prediction tools and indirect results and are not confirmed by PubMed:15689995 and PubMed:15689994. The relevance of PQQ-binding is therefore unclear in vivo.Curated
In invertebrates, aminoadipate-semialdehyde dehydrogenase reaction is a key step of the L-lysine biosynthesis pathway, which is not fully conserved in vertebrates. It has been suggested by PubMed:12712191 that this protein participates in the reverse reaction (i.e. in lysine catabolism), however the relevance of this catalytic activity in vivo remains unclear.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.