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Protein

Protein N-terminal glutamine amidohydrolase

Gene

Wdyhv1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei30 – 301Curated
Active sitei83 – 831Curated
Active sitei99 – 991Curated

GO - Molecular functioni

  1. protein-N-terminal glutamine amidohydrolase activity Source: UniProtKB

GO - Biological processi

  1. cellular protein modification process Source: UniProtKB
  2. protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-terminal glutamine amidohydrolase (EC:3.5.1.-)
Alternative name(s):
Protein NH2-terminal glutamine deamidase
Short name:
N-terminal Gln amidase
Short name:
Nt(Q)-amidase
WDYHV motif-containing protein 1
Gene namesi
Name:Wdyhv1
Synonyms:Ntaq1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1924023. Wdyhv1.

Subcellular locationi

  1. Cytoplasmcytosol 1 Publication
  2. Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi23 – 231C → A: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi28 – 281C → A: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi30 – 301C → A: Loss of N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi38 – 381C → A: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi39 – 391E → Q: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi81 – 811D → N: Impaired N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi83 – 831H → A: Loss of N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi147 – 1471D → N: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Protein N-terminal glutamine amidohydrolasePRO_0000279410Add
BLAST

Proteomic databases

MaxQBiQ80WB5.
PaxDbiQ80WB5.
PRIDEiQ80WB5.

PTM databases

PhosphoSiteiQ80WB5.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ80WB5.
GenevestigatoriQ80WB5.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

SMRiQ80WB5. Positions 10-204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NTAQ1 family.Curated

Phylogenomic databases

eggNOGiNOG282583.
GeneTreeiENSGT00390000014398.
HOGENOMiHOG000007890.
InParanoidiQ80WB5.
OMAiVIHAPVF.
OrthoDBiEOG70PC0N.
PhylomeDBiQ80WB5.
TreeFamiTF105807.

Family and domain databases

Gene3Di3.10.620.10. 1 hit.
InterProiIPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view]
PfamiPF09764. Nt_Gln_amidase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80WB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGDGPAATA PQYQPVCPTR DACVYNSCYC EENIWKLCEY IKTHNQYLLE
60 70 80 90 100
ECYAVFISNE KKMVPIWKQQ ARPENGPVIW DYHVVLLHVS REGQSFIYDL
110 120 130 140 150
DTILPFPCPF DIYIEDALKS DDDIHLQFRR KFRVVRADSY LKHFASDRSH
160 170 180 190 200
MKDSSGNWRE PPPEYPCIET GDSKMNLNDF ISMDPAVGWG AVYTLPEFVH

RFSSKTYQA
Length:209
Mass (Da):24,337
Last modified:June 1, 2003 - v1
Checksum:i789A08A3B94619CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150578 mRNA. Translation: BAE29673.1.
BC051524 mRNA. Translation: AAH51524.1.
CCDSiCCDS27490.1.
RefSeqiNP_084010.1. NM_029734.1.
UniGeneiMm.26771.

Genome annotation databases

EnsembliENSMUST00000067563; ENSMUSP00000064622; ENSMUSG00000022359.
GeneIDi76773.
KEGGimmu:76773.
UCSCiuc007vti.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150578 mRNA. Translation: BAE29673.1.
BC051524 mRNA. Translation: AAH51524.1.
CCDSiCCDS27490.1.
RefSeqiNP_084010.1. NM_029734.1.
UniGeneiMm.26771.

3D structure databases

SMRiQ80WB5. Positions 10-204.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ80WB5.

Proteomic databases

MaxQBiQ80WB5.
PaxDbiQ80WB5.
PRIDEiQ80WB5.

Protocols and materials databases

DNASUi76773.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067563; ENSMUSP00000064622; ENSMUSG00000022359.
GeneIDi76773.
KEGGimmu:76773.
UCSCiuc007vti.1. mouse.

Organism-specific databases

CTDi55093.
MGIiMGI:1924023. Wdyhv1.

Phylogenomic databases

eggNOGiNOG282583.
GeneTreeiENSGT00390000014398.
HOGENOMiHOG000007890.
InParanoidiQ80WB5.
OMAiVIHAPVF.
OrthoDBiEOG70PC0N.
PhylomeDBiQ80WB5.
TreeFamiTF105807.

Miscellaneous databases

NextBioi345775.
PROiQ80WB5.
SOURCEiSearch...

Gene expression databases

BgeeiQ80WB5.
GenevestigatoriQ80WB5.

Family and domain databases

Gene3Di3.10.620.10. 1 hit.
InterProiIPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view]
PfamiPF09764. Nt_Gln_amidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  3. "Glutamine-specific N-terminal amidase, a component of the N-end rule pathway."
    Wang H., Piatkov K.I., Brower C.S., Varshavsky A.
    Mol. Cell 34:686-695(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF CYS-23; CYS-28; CYS-30; CYS-38; GLU-39; ASP-81; HIS-83 AND ASP-147.

Entry informationi

Entry nameiNTAQ1_MOUSE
AccessioniPrimary (citable) accession number: Q80WB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: June 1, 2003
Last modified: April 1, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.