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Q80WB5

- NTAQ1_MOUSE

UniProt

Q80WB5 - NTAQ1_MOUSE

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Protein
Protein N-terminal glutamine amidohydrolase
Gene
Wdyhv1, Ntaq1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei30 – 301 Inferred
Active sitei83 – 831 Inferred
Active sitei99 – 991 Inferred

GO - Molecular functioni

  1. protein-N-terminal glutamine amidohydrolase activity Source: UniProtKB

GO - Biological processi

  1. cellular protein modification process Source: UniProtKB
  2. protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-terminal glutamine amidohydrolase (EC:3.5.1.-)
Alternative name(s):
Protein NH2-terminal glutamine deamidase
Short name:
N-terminal Gln amidase
Short name:
Nt(Q)-amidase
WDYHV motif-containing protein 1
Gene namesi
Name:Wdyhv1
Synonyms:Ntaq1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1924023. Wdyhv1.

Subcellular locationi

Cytoplasmcytosol. Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi23 – 231C → A: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi28 – 281C → A: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi30 – 301C → A: Loss of N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi38 – 381C → A: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi39 – 391E → Q: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi81 – 811D → N: Impaired N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi83 – 831H → A: Loss of N-terminal glutamine amidohydrolase activity. 1 Publication
Mutagenesisi147 – 1471D → N: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Protein N-terminal glutamine amidohydrolase
PRO_0000279410Add
BLAST

Proteomic databases

MaxQBiQ80WB5.
PaxDbiQ80WB5.
PRIDEiQ80WB5.

PTM databases

PhosphoSiteiQ80WB5.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ80WB5.
GenevestigatoriQ80WB5.

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ80WB5.
SMRiQ80WB5. Positions 10-204.

Family & Domainsi

Sequence similaritiesi

Belongs to the NTAQ1 family.

Phylogenomic databases

eggNOGiNOG282583.
GeneTreeiENSGT00390000014398.
HOGENOMiHOG000007890.
InParanoidiQ80WB5.
OMAiNSCYCEE.
OrthoDBiEOG70PC0N.
PhylomeDBiQ80WB5.
TreeFamiTF105807.

Family and domain databases

Gene3Di3.10.620.10. 1 hit.
InterProiIPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view]
PfamiPF09764. Nt_Gln_amidase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80WB5-1 [UniParc]FASTAAdd to Basket

« Hide

MEGDGPAATA PQYQPVCPTR DACVYNSCYC EENIWKLCEY IKTHNQYLLE    50
ECYAVFISNE KKMVPIWKQQ ARPENGPVIW DYHVVLLHVS REGQSFIYDL 100
DTILPFPCPF DIYIEDALKS DDDIHLQFRR KFRVVRADSY LKHFASDRSH 150
MKDSSGNWRE PPPEYPCIET GDSKMNLNDF ISMDPAVGWG AVYTLPEFVH 200
RFSSKTYQA 209
Length:209
Mass (Da):24,337
Last modified:June 1, 2003 - v1
Checksum:i789A08A3B94619CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK150578 mRNA. Translation: BAE29673.1.
BC051524 mRNA. Translation: AAH51524.1.
CCDSiCCDS27490.1.
RefSeqiNP_084010.1. NM_029734.1.
UniGeneiMm.26771.

Genome annotation databases

EnsembliENSMUST00000067563; ENSMUSP00000064622; ENSMUSG00000022359.
GeneIDi76773.
KEGGimmu:76773.
UCSCiuc007vti.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK150578 mRNA. Translation: BAE29673.1 .
BC051524 mRNA. Translation: AAH51524.1 .
CCDSi CCDS27490.1.
RefSeqi NP_084010.1. NM_029734.1.
UniGenei Mm.26771.

3D structure databases

ProteinModelPortali Q80WB5.
SMRi Q80WB5. Positions 10-204.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q80WB5.

Proteomic databases

MaxQBi Q80WB5.
PaxDbi Q80WB5.
PRIDEi Q80WB5.

Protocols and materials databases

DNASUi 76773.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000067563 ; ENSMUSP00000064622 ; ENSMUSG00000022359 .
GeneIDi 76773.
KEGGi mmu:76773.
UCSCi uc007vti.1. mouse.

Organism-specific databases

CTDi 55093.
MGIi MGI:1924023. Wdyhv1.

Phylogenomic databases

eggNOGi NOG282583.
GeneTreei ENSGT00390000014398.
HOGENOMi HOG000007890.
InParanoidi Q80WB5.
OMAi NSCYCEE.
OrthoDBi EOG70PC0N.
PhylomeDBi Q80WB5.
TreeFami TF105807.

Miscellaneous databases

NextBioi 345775.
PROi Q80WB5.
SOURCEi Search...

Gene expression databases

Bgeei Q80WB5.
Genevestigatori Q80WB5.

Family and domain databases

Gene3Di 3.10.620.10. 1 hit.
InterProi IPR023128. Prot_N_Gln_amidohydro_ab_roll.
[Graphical view ]
Pfami PF09764. Nt_Gln_amidase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  3. "Glutamine-specific N-terminal amidase, a component of the N-end rule pathway."
    Wang H., Piatkov K.I., Brower C.S., Varshavsky A.
    Mol. Cell 34:686-695(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF CYS-23; CYS-28; CYS-30; CYS-38; GLU-39; ASP-81; HIS-83 AND ASP-147.

Entry informationi

Entry nameiNTAQ1_MOUSE
AccessioniPrimary (citable) accession number: Q80WB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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