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Q80WB5

- NTAQ1_MOUSE

UniProt

Q80WB5 - NTAQ1_MOUSE

Protein

Protein N-terminal glutamine amidohydrolase

Gene

Wdyhv1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei30 – 301Curated
    Active sitei83 – 831Curated
    Active sitei99 – 991Curated

    GO - Molecular functioni

    1. protein-N-terminal glutamine amidohydrolase activity Source: UniProtKB

    GO - Biological processi

    1. cellular protein modification process Source: UniProtKB
    2. protein catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein N-terminal glutamine amidohydrolase (EC:3.5.1.-)
    Alternative name(s):
    Protein NH2-terminal glutamine deamidase
    Short name:
    N-terminal Gln amidase
    Short name:
    Nt(Q)-amidase
    WDYHV motif-containing protein 1
    Gene namesi
    Name:Wdyhv1
    Synonyms:Ntaq1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1924023. Wdyhv1.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi23 – 231C → A: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
    Mutagenesisi28 – 281C → A: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
    Mutagenesisi30 – 301C → A: Loss of N-terminal glutamine amidohydrolase activity. 1 Publication
    Mutagenesisi38 – 381C → A: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
    Mutagenesisi39 – 391E → Q: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication
    Mutagenesisi81 – 811D → N: Impaired N-terminal glutamine amidohydrolase activity. 1 Publication
    Mutagenesisi83 – 831H → A: Loss of N-terminal glutamine amidohydrolase activity. 1 Publication
    Mutagenesisi147 – 1471D → N: Does not strongly affect N-terminal glutamine amidohydrolase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 209209Protein N-terminal glutamine amidohydrolasePRO_0000279410Add
    BLAST

    Proteomic databases

    MaxQBiQ80WB5.
    PaxDbiQ80WB5.
    PRIDEiQ80WB5.

    PTM databases

    PhosphoSiteiQ80WB5.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    BgeeiQ80WB5.
    GenevestigatoriQ80WB5.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ80WB5.
    SMRiQ80WB5. Positions 10-204.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NTAQ1 family.Curated

    Phylogenomic databases

    eggNOGiNOG282583.
    GeneTreeiENSGT00390000014398.
    HOGENOMiHOG000007890.
    InParanoidiQ80WB5.
    OMAiNSCYCEE.
    OrthoDBiEOG70PC0N.
    PhylomeDBiQ80WB5.
    TreeFamiTF105807.

    Family and domain databases

    Gene3Di3.10.620.10. 1 hit.
    InterProiIPR023128. Prot_N_Gln_amidohydro_ab_roll.
    [Graphical view]
    PfamiPF09764. Nt_Gln_amidase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80WB5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGDGPAATA PQYQPVCPTR DACVYNSCYC EENIWKLCEY IKTHNQYLLE    50
    ECYAVFISNE KKMVPIWKQQ ARPENGPVIW DYHVVLLHVS REGQSFIYDL 100
    DTILPFPCPF DIYIEDALKS DDDIHLQFRR KFRVVRADSY LKHFASDRSH 150
    MKDSSGNWRE PPPEYPCIET GDSKMNLNDF ISMDPAVGWG AVYTLPEFVH 200
    RFSSKTYQA 209
    Length:209
    Mass (Da):24,337
    Last modified:June 1, 2003 - v1
    Checksum:i789A08A3B94619CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK150578 mRNA. Translation: BAE29673.1.
    BC051524 mRNA. Translation: AAH51524.1.
    CCDSiCCDS27490.1.
    RefSeqiNP_084010.1. NM_029734.1.
    UniGeneiMm.26771.

    Genome annotation databases

    EnsembliENSMUST00000067563; ENSMUSP00000064622; ENSMUSG00000022359.
    GeneIDi76773.
    KEGGimmu:76773.
    UCSCiuc007vti.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK150578 mRNA. Translation: BAE29673.1 .
    BC051524 mRNA. Translation: AAH51524.1 .
    CCDSi CCDS27490.1.
    RefSeqi NP_084010.1. NM_029734.1.
    UniGenei Mm.26771.

    3D structure databases

    ProteinModelPortali Q80WB5.
    SMRi Q80WB5. Positions 10-204.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q80WB5.

    Proteomic databases

    MaxQBi Q80WB5.
    PaxDbi Q80WB5.
    PRIDEi Q80WB5.

    Protocols and materials databases

    DNASUi 76773.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000067563 ; ENSMUSP00000064622 ; ENSMUSG00000022359 .
    GeneIDi 76773.
    KEGGi mmu:76773.
    UCSCi uc007vti.1. mouse.

    Organism-specific databases

    CTDi 55093.
    MGIi MGI:1924023. Wdyhv1.

    Phylogenomic databases

    eggNOGi NOG282583.
    GeneTreei ENSGT00390000014398.
    HOGENOMi HOG000007890.
    InParanoidi Q80WB5.
    OMAi NSCYCEE.
    OrthoDBi EOG70PC0N.
    PhylomeDBi Q80WB5.
    TreeFami TF105807.

    Miscellaneous databases

    NextBioi 345775.
    PROi Q80WB5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q80WB5.
    Genevestigatori Q80WB5.

    Family and domain databases

    Gene3Di 3.10.620.10. 1 hit.
    InterProi IPR023128. Prot_N_Gln_amidohydro_ab_roll.
    [Graphical view ]
    Pfami PF09764. Nt_Gln_amidase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Bone marrow.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    3. "Glutamine-specific N-terminal amidase, a component of the N-end rule pathway."
      Wang H., Piatkov K.I., Brower C.S., Varshavsky A.
      Mol. Cell 34:686-695(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF CYS-23; CYS-28; CYS-30; CYS-38; GLU-39; ASP-81; HIS-83 AND ASP-147.

    Entry informationi

    Entry nameiNTAQ1_MOUSE
    AccessioniPrimary (citable) accession number: Q80WB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2007
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3