Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cation channel sperm-associated protein 3

Gene

Catsper3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated calcium channel that plays a central role in sperm cell hyperactivation. Controls calcium entry to mediate the hyperactivated motility, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Activated by intracellular alkalinization.2 Publications

Enzyme regulationi

In contrast to the human ortholog, not activated by progesterone.1 Publication

GO - Molecular functioni

  • voltage-gated calcium channel activity Source: MGI

GO - Biological processi

  • membrane depolarization during action potential Source: GO_Central
  • multicellular organism development Source: UniProtKB-KW
  • sodium ion transport Source: MGI
  • sperm capacitation Source: MGI
  • sperm motility Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Developmental protein, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Differentiation, Ion transport, Spermatogenesis, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-1300642. Sperm Motility And Taxes.

Protein family/group databases

TCDBi1.A.1.19.3. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cation channel sperm-associated protein 3
Short name:
CatSper3
Gene namesi
Name:Catsper3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1924106. Catsper3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4949CytoplasmicSequence analysisAdd
BLAST
Transmembranei50 – 7021Helical; Name=Segment S1Sequence analysisAdd
BLAST
Topological domaini71 – 8717ExtracellularSequence analysisAdd
BLAST
Transmembranei88 – 10821Helical; Name=Segment S2Sequence analysisAdd
BLAST
Topological domaini109 – 1102CytoplasmicSequence analysis
Transmembranei111 – 13121Helical; Name=Segment S3Sequence analysisAdd
BLAST
Topological domaini132 – 14110ExtracellularSequence analysis
Transmembranei142 – 16120Helical; Name=Segment S4Sequence analysisAdd
BLAST
Topological domaini162 – 17918CytoplasmicSequence analysisAdd
BLAST
Transmembranei180 – 20021Helical; Name=Segment S5Sequence analysisAdd
BLAST
Topological domaini201 – 2099ExtracellularSequence analysis
Intramembranei210 – 23021Helical; Pore-formingSequence analysisAdd
BLAST
Topological domaini231 – 24818ExtracellularSequence analysisAdd
BLAST
Transmembranei249 – 26921Helical; Name=Segment S6Sequence analysisAdd
BLAST
Topological domaini270 – 395126CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • acrosomal vesicle Source: MGI
  • CatSper complex Source: UniProtKB
  • endoplasmic reticulum Source: MGI
  • motile cilium Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Flagellum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are normal but males are sterile. Male sterility is due to defects in sperm motility unability to fertilize intact eggs.2 Publications

Chemistry

GuidetoPHARMACOLOGYi390.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Cation channel sperm-associated protein 3PRO_0000295680Add
BLAST

Proteomic databases

PaxDbiQ80W99.
PRIDEiQ80W99.

PTM databases

PhosphoSiteiQ80W99.

Expressioni

Tissue specificityi

Testis-specific.2 Publications

Developmental stagei

Detected in hte testis during postnatal development at day 15. Restricted to the late-stage germline cells that line the seminiferous tubules.1 Publication

Gene expression databases

BgeeiQ80W99.
CleanExiMM_CATSPER3.
ExpressionAtlasiQ80W99. baseline and differential.
GenevisibleiQ80W99. MM.

Interactioni

Subunit structurei

Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4 (Potential). Component of the CatSper complex. Interacts with CATSPER1.Curated2 Publications

Protein-protein interaction databases

BioGridi218352. 1 interaction.
DIPiDIP-60802N.
STRINGi10090.ENSMUSP00000021961.

Structurei

3D structure databases

ProteinModelPortaliQ80W99.
SMRiQ80W99. Positions 42-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF9A. Eukaryota.
ENOG4111SVN. LUCA.
GeneTreeiENSGT00760000119325.
HOGENOMiHOG000112052.
HOVERGENiHBG099875.
InParanoidiQ80W99.
KOiK16891.
OMAiYWKDGYN.
OrthoDBiEOG7PVWQD.
PhylomeDBiQ80W99.
TreeFamiTF343841.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
[Graphical view]
PfamiPF00520. Ion_trans. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80W99-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQHFHHNPV RVKSGSLFAT ASEALQARLS KIKRKDKECQ AYFRKVIKST
60 70 80 90 100
FFQIVMITTV TTNSFLLVLG TNYDIQFEFF RTFEVSELFF VSVYVCEFLM
110 120 130 140 150
KVYVDPITYW KDGYNILDVI ILIILTIPYL LRKIKGNHSA YLHFADGIQS
160 170 180 190 200
LRILKLISYS RGIRTLIIAV GETVYTVASV LTLLFLLMFV FAILGFCLFG
210 220 230 240 250
VTDRGDLENW GNLASAFFTL FSLATVDGWT DLQEELDKRK FTVSRAFTIL
260 270 280 290 300
FILLASFIFL NMFVGVMIMH TEDSMKKFER DLTLERNLAI MEEKQIILKR
310 320 330 340 350
QQEEVNRLMN TQKSGSMNFI DMVEGFKKTL RHTDPMVLDD FSTSLSFIDI
360 370 380 390
YLVTLDNQDV IVSKLQELYC EIVNVLSLML EDMPKESSSS LSGLS
Length:395
Mass (Da):45,486
Last modified:July 24, 2007 - v2
Checksum:i10641E91039210A6
GO
Isoform 2 (identifier: Q80W99-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     84-96: Missing.

Note: No experimental confirmation available.
Show »
Length:382
Mass (Da):43,984
Checksum:i3DA71D5110E9D629
GO

Sequence cautioni

The sequence AAH89518.1 differs from that shown.Contaminating sequence.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti314 – 3141S → T in AAP21831 (PubMed:17227845).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei84 – 9613Missing in isoform 2. 1 PublicationVSP_026978Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY263400 mRNA. Translation: AAP21831.1.
AK014942 mRNA. Translation: BAB29631.1.
BC089518 mRNA. Translation: AAH89518.1. Sequence problems.
CCDSiCCDS26555.1. [Q80W99-2]
CCDS56885.1. [Q80W99-1]
RefSeqiNP_001239416.1. NM_001252487.1. [Q80W99-1]
NP_084048.1. NM_029772.4. [Q80W99-2]
XP_006517518.1. XM_006517455.2. [Q80W99-1]
UniGeneiMm.159795.

Genome annotation databases

EnsembliENSMUST00000021961; ENSMUSP00000021961; ENSMUSG00000021499. [Q80W99-1]
ENSMUST00000109898; ENSMUSP00000105524; ENSMUSG00000021499. [Q80W99-2]
GeneIDi76856.
KEGGimmu:76856.
UCSCiuc007qsb.2. mouse. [Q80W99-2]
uc007qsc.2. mouse. [Q80W99-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY263400 mRNA. Translation: AAP21831.1.
AK014942 mRNA. Translation: BAB29631.1.
BC089518 mRNA. Translation: AAH89518.1. Sequence problems.
CCDSiCCDS26555.1. [Q80W99-2]
CCDS56885.1. [Q80W99-1]
RefSeqiNP_001239416.1. NM_001252487.1. [Q80W99-1]
NP_084048.1. NM_029772.4. [Q80W99-2]
XP_006517518.1. XM_006517455.2. [Q80W99-1]
UniGeneiMm.159795.

3D structure databases

ProteinModelPortaliQ80W99.
SMRiQ80W99. Positions 42-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218352. 1 interaction.
DIPiDIP-60802N.
STRINGi10090.ENSMUSP00000021961.

Chemistry

GuidetoPHARMACOLOGYi390.

Protein family/group databases

TCDBi1.A.1.19.3. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteiQ80W99.

Proteomic databases

PaxDbiQ80W99.
PRIDEiQ80W99.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021961; ENSMUSP00000021961; ENSMUSG00000021499. [Q80W99-1]
ENSMUST00000109898; ENSMUSP00000105524; ENSMUSG00000021499. [Q80W99-2]
GeneIDi76856.
KEGGimmu:76856.
UCSCiuc007qsb.2. mouse. [Q80W99-2]
uc007qsc.2. mouse. [Q80W99-1]

Organism-specific databases

CTDi347732.
MGIiMGI:1924106. Catsper3.

Phylogenomic databases

eggNOGiENOG410IF9A. Eukaryota.
ENOG4111SVN. LUCA.
GeneTreeiENSGT00760000119325.
HOGENOMiHOG000112052.
HOVERGENiHBG099875.
InParanoidiQ80W99.
KOiK16891.
OMAiYWKDGYN.
OrthoDBiEOG7PVWQD.
PhylomeDBiQ80W99.
TreeFamiTF343841.

Enzyme and pathway databases

ReactomeiR-MMU-1300642. Sperm Motility And Taxes.

Miscellaneous databases

PROiQ80W99.
SOURCEiSearch...

Gene expression databases

BgeeiQ80W99.
CleanExiMM_CATSPER3.
ExpressionAtlasiQ80W99. baseline and differential.
GenevisibleiQ80W99. MM.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
[Graphical view]
PfamiPF00520. Ion_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility."
    Qi H., Moran M.M., Navarro B., Chong J.A., Krapivinsky G., Krapivinsky L., Kirichok Y., Ramsey I.S., Quill T.A., Clapham D.E.
    Proc. Natl. Acad. Sci. U.S.A. 104:1219-1223(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH CATSPER1, DISRUPTION PHENOTYPE.
    Strain: BALB/cJ.
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  4. "Catsper3 and catsper4 encode two cation channel-like proteins exclusively expressed in the testis."
    Jin J.-L., O'Doherty A.M., Wang S., Zheng H., Sanders K.M., Yan W.
    Biol. Reprod. 73:1235-1242(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility."
    Li H.-G., Ding X.-F., Liao A.-H., Kong X.-B., Xiong C.-L.
    Mol. Hum. Reprod. 13:299-306(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. "Catsper3 and catsper4 are essential for sperm hyperactivated motility and male fertility in the mouse."
    Jin J.-L., Jin N., Zheng H., Ro S., Tafolla D., Sanders K.M., Yan W.
    Biol. Reprod. 77:37-44(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "Progesterone activates the principal Ca2+ channel of human sperm."
    Lishko P.V., Botchkina I.L., Kirichok Y.
    Nature 471:387-391(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF ACTIVATION BY PROGESTERONE AND PGE1.
  8. "A novel gene required for male fertility and functional CATSPER channel formation in spermatozoa."
    Chung J.J., Navarro B., Krapivinsky G., Krapivinsky L., Clapham D.E.
    Nat. Commun. 2:153-153(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CATSPER COMPLEX.
    Strain: C57BL/6J.

Entry informationi

Entry nameiCTSR3_MOUSE
AccessioniPrimary (citable) accession number: Q80W99
Secondary accession number(s): Q5FWA9, Q9D5T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: July 6, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.