Reviewed, UniProtKB/Swiss-Prot Q80W94 (MOGT2_MOUSE)

Names and origin

Protein names

Recommended name:
    2-acylglycerol O-acyltransferase 2
    EC=2.3.1.22
Alternative name(s):
    Monoacylglycerol O-acyltransferase 2
    Acyl CoA:monoacylglycerol acyltransferase 2
      Short name=MGAT2
    Diacylglycerol acyltransferase 2-like protein 5
    Monoacylglycerol O-acyltransferase 1-like

Gene names

Name:	Mogat2
Synonyms:	Dgat2l5, Mgat1l

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.
Catalytic activity	Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol.
Enzyme regulation	Inhibited by oleic acid and sphingosine, while it is stimulated by phosphatidylcholine, phosphatidylserine and phosphatidic acid.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Mainly expressed in small intestine. Detected in the small intestine in a proximal-to-distal gradient that correlated with fat absorption pattern. Present not only in the villi, but also in the crypt regions of the small intestine, which suggests that expression occurs prior to the maturation of enterocytes. Not detectable in other sections of the digestive tract, including stomach, cecum, colon and rectum, or other tissues such as kidney, liver and adipocytes (at protein level). Also detected in kidney, adipose and stomach. Expressed at very low level in liver, skeletal muscle and spleen. Not expressed in brain, heart, lung, skin, testis and thymus.
Induction	Up-regulated in mice fed a high fat diet, implicating a role in diet-induced obesity (at protein level).
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0.

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	diacylglycerol biosynthetic process Ref.4 Ref.6 Inferred from direct assay. Source: MGI glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW intestinal absorption Ref.6 Inferred from direct assay. Source: MGI triacylglycerol biosynthetic process Ref.4 Inferred from direct assay. Source: MGI
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-acylglycerol O-acyltransferase activity Ref.6 Inferred from direct assay. Source: MGI acetyltransferase activity Ref.4 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	2-acylglycerol O-acyltransferase 2		PRO_0000249063
Regions
	Transmembrane	23 – 43	21	Potential
	Transmembrane	212 – 232	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q80W94-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: BFC7C84A22B607BD	FASTA	334	38,591
	10 20 30 40 50 60 MVEFAPLLVP WERRLQTFAV LQWVFSFLAL AQLCIVIFVG LLFTRFWLFS VLYATWWYLD 70 80 90 100 110 120 WDKPRQGGRP IQFFRRLAIW KYMKDYFPVS LVKTAELDPS RNYIAGFHPH GVLAAGAFLN 130 140 150 160 170 180 LCTESTGFTS LFPGIRSYLM MLTVWFRAPF FRDYIMSGGL VSSEKVSADH ILSRKGGGNL 190 200 210 220 230 240 LAIIVGGAQE ALDARPGAYR LLLKNRKGFI RLALMHGAAL VPIFSFGENN LFNQVENTPG 250 260 270 280 290 300 TWLRWIQNRL QKIMGISLPL FHGRGVFQYS FGLMPFRQPI TTIVGKPIEV QMTPQPSREE 310 320 330 VDRLHQRYIK ELCKLFEEHK LKFNVPEDQH LEFC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MGAT2, a monoacylglycerol acyltransferase expressed in the small intestine." Yen C.-L.E., Farese R.V. Jr. J. Biol. Chem. 278:18532-18537(2003) [PubMed: 12621063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: C57BL/6J. Tissue: Intestine.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6NCr. Tissue: Hematopoietic stem cell.
[4]	"Cloning and functional characterization of a mouse intestinal acyl-CoA:monoacylglycerol acyltransferase, MGAT2." Cao J., Lockwood J., Burn P., Shi Y. J. Biol. Chem. 278:13860-13866(2003) [PubMed: 12576479] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	"Properties of the mouse intestinal acyl-CoA:monoacylglycerol acyltransferase, MGAT2." Cao J., Burn P., Shi Y. J. Biol. Chem. 278:25657-25663(2003) [PubMed: 12730219] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"A predominant role of acyl-CoA:monoacylglycerol acyltransferase-2 in dietary fat absorption implicated by tissue distribution, subcellular localization, and up-regulation by high fat diet." Cao J., Hawkins E., Brozinick J., Liu X., Zhang H., Burn P., Shi Y. J. Biol. Chem. 279:18878-18886(2004) [PubMed: 14966132] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY157609 mRNA. Translation: AAO23673.1. AK133854 mRNA. Translation: BAE21888.1. BC052831 mRNA. Translation: AAH52831.1.
RefSeq	NP_803231.1.
UniGene	Mm.208030
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q80W94.
Genome annotation databases
Ensembl	ENSMUSG00000052396. Mus musculus. [Contig view]
GeneID	233549.
KEGG	mmu:233549.
Organism-specific databases
MGI	MGI:2663253. Mogat2.
Phylogenomic databases
HOVERGEN	Q80W94.
Gene expression databases
ArrayExpress	Q80W94.
CleanEx	MM_MOGAT2.
GermOnline	ENSMUSG00000052396. Mus musculus.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
PANTHER	PTHR12317. DAGAT. 1 hit.
Pfam	PF03982. DAGAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	381755.
SOURCE	Search...

Entry information

Entry name

MOGT2_MOUSE

Accession

Primary (citable) accession number: Q80W94

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)