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Protein

2-acylglycerol O-acyltransferase 2

Gene

Mogat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.3 Publications

Catalytic activityi

Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol.

Enzyme regulationi

Inhibited by oleic acid and sphingosine, while it is stimulated by phosphatidylcholine, phosphatidylserine and phosphatidic acid.1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Pathwayi: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

GO - Molecular functioni

  • 2-acylglycerol O-acyltransferase activity Source: MGI
  • acetyltransferase activity Source: MGI

GO - Biological processi

  • diacylglycerol biosynthetic process Source: MGI
  • glycerol metabolic process Source: UniProtKB-KW
  • intestinal absorption Source: MGI
  • lipid metabolic process Source: MGI
  • monoacylglycerol metabolic process Source: MGI
  • triglyceride biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00282.

Names & Taxonomyi

Protein namesi
Recommended name:
2-acylglycerol O-acyltransferase 2 (EC:2.3.1.22)
Alternative name(s):
Acyl-CoA:monoacylglycerol acyltransferase 2
Short name:
MGAT2
Diacylglycerol acyltransferase 2-like protein 5
Monoacylglycerol O-acyltransferase 1-like
Monoacylglycerol O-acyltransferase 2
Gene namesi
Name:Mogat2
Synonyms:Dgat2l5, Mgat1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2663253. Mogat2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Transmembranei212 – 23221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3343342-acylglycerol O-acyltransferase 2PRO_0000249063Add
BLAST

Proteomic databases

MaxQBiQ80W94.
PaxDbiQ80W94.
PeptideAtlasiQ80W94.
PRIDEiQ80W94.

PTM databases

iPTMnetiQ80W94.
PhosphoSiteiQ80W94.

Expressioni

Tissue specificityi

Mainly expressed in small intestine. Detected in the small intestine in a proximal-to-distal gradient that correlated with fat absorption pattern. Present not only in the villi, but also in the crypt regions of the small intestine, which suggests that expression occurs prior to the maturation of enterocytes. Not detectable in other sections of the digestive tract, including stomach, cecum, colon and rectum, or other tissues such as kidney, liver and adipocytes (at protein level). Also detected in kidney, adipose and stomach. Expressed at very low level in liver, skeletal muscle and spleen. Not expressed in brain, heart, lung, skin, testis and thymus.3 Publications

Inductioni

Up-regulated in mice fed a high fat diet, implicating a role in diet-induced obesity (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000052396.
CleanExiMM_MOGAT2.
GenevisibleiQ80W94. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000064041.

Structurei

3D structure databases

ProteinModelPortaliQ80W94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
HOVERGENiHBG065791.
InParanoidiQ80W94.
KOiK14457.
OMAiLALAQIC.
OrthoDBiEOG091G0BGQ.
PhylomeDBiQ80W94.
TreeFamiTF314707.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80W94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEFAPLLVP WERRLQTFAV LQWVFSFLAL AQLCIVIFVG LLFTRFWLFS
60 70 80 90 100
VLYATWWYLD WDKPRQGGRP IQFFRRLAIW KYMKDYFPVS LVKTAELDPS
110 120 130 140 150
RNYIAGFHPH GVLAAGAFLN LCTESTGFTS LFPGIRSYLM MLTVWFRAPF
160 170 180 190 200
FRDYIMSGGL VSSEKVSADH ILSRKGGGNL LAIIVGGAQE ALDARPGAYR
210 220 230 240 250
LLLKNRKGFI RLALMHGAAL VPIFSFGENN LFNQVENTPG TWLRWIQNRL
260 270 280 290 300
QKIMGISLPL FHGRGVFQYS FGLMPFRQPI TTIVGKPIEV QMTPQPSREE
310 320 330
VDRLHQRYIK ELCKLFEEHK LKFNVPEDQH LEFC
Length:334
Mass (Da):38,591
Last modified:June 1, 2003 - v1
Checksum:iBFC7C84A22B607BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157609 mRNA. Translation: AAO23673.1.
AK133854 mRNA. Translation: BAE21888.1.
BC052831 mRNA. Translation: AAH52831.1.
CCDSiCCDS21478.1.
RefSeqiNP_803231.1. NM_177448.4.
UniGeneiMm.208030.

Genome annotation databases

EnsembliENSMUST00000064231; ENSMUSP00000064041; ENSMUSG00000052396.
GeneIDi233549.
KEGGimmu:233549.
UCSCiuc009ilf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157609 mRNA. Translation: AAO23673.1.
AK133854 mRNA. Translation: BAE21888.1.
BC052831 mRNA. Translation: AAH52831.1.
CCDSiCCDS21478.1.
RefSeqiNP_803231.1. NM_177448.4.
UniGeneiMm.208030.

3D structure databases

ProteinModelPortaliQ80W94.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000064041.

PTM databases

iPTMnetiQ80W94.
PhosphoSiteiQ80W94.

Proteomic databases

MaxQBiQ80W94.
PaxDbiQ80W94.
PeptideAtlasiQ80W94.
PRIDEiQ80W94.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064231; ENSMUSP00000064041; ENSMUSG00000052396.
GeneIDi233549.
KEGGimmu:233549.
UCSCiuc009ilf.2. mouse.

Organism-specific databases

CTDi80168.
MGIiMGI:2663253. Mogat2.

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
HOVERGENiHBG065791.
InParanoidiQ80W94.
KOiK14457.
OMAiLALAQIC.
OrthoDBiEOG091G0BGQ.
PhylomeDBiQ80W94.
TreeFamiTF314707.

Enzyme and pathway databases

UniPathwayiUPA00282.
ReactomeiR-MMU-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ80W94.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052396.
CleanExiMM_MOGAT2.
GenevisibleiQ80W94. MM.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOGT2_MOUSE
AccessioniPrimary (citable) accession number: Q80W94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.