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Protein

Protein VAC14 homolog

Gene

Vac14

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 5-phosphate (PtdIns5P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-1660514. Synthesis of PIPs at the Golgi membrane.
R-RNO-1660516. Synthesis of PIPs at the early endosome membrane.
R-RNO-1660517. Synthesis of PIPs at the late endosome membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VAC14 homolog
Gene namesi
Name:Vac14
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi631410. Vac14.

Subcellular locationi

  • Endosome membrane By similarity
  • Microsome membrane 1 Publication

  • Note: Mainly associated with membranes of the late endocytic pathway.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi781 – 7833VVL → AAA: Reduced interaction with NOS1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 783783Protein VAC14 homologPRO_0000300487Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei11 – 111PhosphothreonineBy similarity
Modified residuei500 – 5001PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ80W92.
PRIDEiQ80W92.

PTM databases

iPTMnetiQ80W92.
PhosphoSiteiQ80W92.

Expressioni

Tissue specificityi

Expressed in brain, lung, kidney and testis with highest levels in brain and testis.1 Publication

Developmental stagei

Expressed in embryonic brain.1 Publication

Gene expression databases

ExpressionAtlasiQ80W92. baseline.
GenevisibleiQ80W92. RN.

Interactioni

Subunit structurei

Forms homooligomers. Component of the PI(3,5)P2 regulatory complex/PAS complex, at least composed of PIKFYVE, FIG4 and VAC14. VAC14 nucleates the assembly of the complex and serves as a scaffold (By similarity). Interacts with NOS1.By similarity1 Publication

Protein-protein interaction databases

BioGridi258827. 1 interaction.
IntActiQ80W92. 1 interaction.
MINTiMINT-3982533.
STRINGi10116.ENSRNOP00000023383.

Structurei

3D structure databases

ProteinModelPortaliQ80W92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati5 – 4238HEAT 1Add
BLAST
Repeati89 – 12638HEAT 2Add
BLAST
Repeati171 – 20838HEAT 3Add
BLAST
Repeati212 – 24938HEAT 4Add
BLAST
Repeati439 – 47638HEAT 5Add
BLAST
Repeati561 – 59939HEAT 6Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni774 – 7785Mediates interaction with the PDZ domain of NOS1

Domaini

The C-terminal domain (residues 524-783) mediates homomeric interactions and is necessary for the formation and maintenance of the PI(3,5)P2 regulatory complex.By similarity

Sequence similaritiesi

Belongs to the VAC14 family.Curated
Contains 6 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0212. Eukaryota.
ENOG410XP6E. LUCA.
GeneTreeiENSGT00390000008385.
HOGENOMiHOG000216640.
HOVERGENiHBG104397.
InParanoidiQ80W92.
KOiK15305.
OMAiCIEKEED.
OrthoDBiEOG7QNVM5.
PhylomeDBiQ80W92.
TreeFamiTF343690.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR026825. Vac14.
IPR032878. Vac14_Fab1-bd.
IPR021841. VAC14_Fig4p-bd.
[Graphical view]
PANTHERiPTHR16023. PTHR16023. 2 hits.
PfamiPF12755. Vac14_Fab1_bd. 1 hit.
PF11916. Vac14_Fig4_bd. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Q80W92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPEKDFAPL TPNIVRALND KLYEKRKVAA LEIEKLVRDF VAQNNTVQIK
60 70 80 90 100
HVIQTLSQEF ALSQHPHSRK GGLIGLAACS IALGKDSGLY LKELIEPVLT
110 120 130 140 150
CFNDADSRLR YYACEALYNI VKVARGAVLP HFNVLFDGLS KLAADPDPNV
160 170 180 190 200
KSGSELLDRL LKDIVTESSK FDLVGFIPLL RERIYSNNQY ARQFIISWIL
210 220 230 240 250
VLVSVPDINL LDYLPEILDG LFQILGDNGK EIRKMCEVVL GEFLKEIKKN
260 270 280 290 300
PSSVKFAEMA NILVIHCQTT DDLIQLTAMC WMREFIQLAG RVMLPYSSGI
310 320 330 340 350
LTAVLPCLAY DDRKKSIKEV ANVCNQSLMK LVTPEDDEPD EPKSVAQKQT
360 370 380 390 400
EPNPEDSLPK QEGTASGGAS GPCDSSFGSG ISVFTSASTD RAPVTLHLDG
410 420 430 440 450
IVQVLNCHLS DTTIGMMTRI AVLKWLYHLY IKTPRKMFRH TDSLFPILLQ
460 470 480 490 500
TLSDESDEVV LKDLEVLAEI ASSPAGQTDD PGAPDGPDLQ VNHSELQVPT
510 520 530 540 550
SGRANLLNPP NTKGLECSPS TPTMNSYFYK FMINLLQTFS SERKLLEARG
560 570 580 590 600
PFIIRQLCLL LNAENIFHSM ADILLREEDL KFASTMVHTL NTILLTSTEL
610 620 630 640 650
FQLRNQLKDL KTLESQNLFC CLYRSWCHNP VTTVSLCFLT QNYRHAYDLI
660 670 680 690 700
QKFGDLEVTV DFLTEVDKLV QLIECPIFTY LRLQLLDVKN NPYLMKALYG
710 720 730 740 750
LLMLLPQSSA FQLLSHRLQC VPNPELLQTE DCLKAAPKSQ KGDSPSIDYT
760 770 780
ELLQHFEKVQ KQHLEVRHQR SGRGDHLDRR VVL
Length:783
Mass (Da):88,068
Last modified:June 1, 2003 - v1
Checksum:i78312BE8D8A83B39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY220476 mRNA. Translation: AAO48767.1.
RefSeqiNP_808791.1. NM_177930.2.
UniGeneiRn.46366.

Genome annotation databases

EnsembliENSRNOT00000023383; ENSRNOP00000023383; ENSRNOG00000017219.
GeneIDi307842.
KEGGirno:307842.
UCSCiRGD:631410. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY220476 mRNA. Translation: AAO48767.1.
RefSeqiNP_808791.1. NM_177930.2.
UniGeneiRn.46366.

3D structure databases

ProteinModelPortaliQ80W92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi258827. 1 interaction.
IntActiQ80W92. 1 interaction.
MINTiMINT-3982533.
STRINGi10116.ENSRNOP00000023383.

PTM databases

iPTMnetiQ80W92.
PhosphoSiteiQ80W92.

Proteomic databases

PaxDbiQ80W92.
PRIDEiQ80W92.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023383; ENSRNOP00000023383; ENSRNOG00000017219.
GeneIDi307842.
KEGGirno:307842.
UCSCiRGD:631410. rat.

Organism-specific databases

CTDi55697.
RGDi631410. Vac14.

Phylogenomic databases

eggNOGiKOG0212. Eukaryota.
ENOG410XP6E. LUCA.
GeneTreeiENSGT00390000008385.
HOGENOMiHOG000216640.
HOVERGENiHBG104397.
InParanoidiQ80W92.
KOiK15305.
OMAiCIEKEED.
OrthoDBiEOG7QNVM5.
PhylomeDBiQ80W92.
TreeFamiTF343690.

Enzyme and pathway databases

ReactomeiR-RNO-1660514. Synthesis of PIPs at the Golgi membrane.
R-RNO-1660516. Synthesis of PIPs at the early endosome membrane.
R-RNO-1660517. Synthesis of PIPs at the late endosome membrane.

Miscellaneous databases

NextBioi657966.
PROiQ80W92.

Gene expression databases

ExpressionAtlasiQ80W92. baseline.
GenevisibleiQ80W92. RN.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR026825. Vac14.
IPR032878. Vac14_Fab1-bd.
IPR021841. VAC14_Fig4p-bd.
[Graphical view]
PANTHERiPTHR16023. PTHR16023. 2 hits.
PfamiPF12755. Vac14_Fab1_bd. 1 hit.
PF11916. Vac14_Fig4_bd. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
ProtoNetiSearch...

Publicationsi

  1. "Congenital hydrocephalus in hy3 mice is caused by a frameshift mutation in Hydin, a large novel gene."
    Davy B.E., Robinson M.L.
    Hum. Mol. Genet. 12:1163-1170(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Binding of Vac14 to neuronal nitric oxide synthase: Characterisation of a new internal PDZ-recognition motif."
    Lemaire J.F., McPherson P.S.
    FEBS Lett. 580:6948-6954(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOS1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF 781-VAL--LEU-783.

Entry informationi

Entry nameiVAC14_RAT
AccessioniPrimary (citable) accession number: Q80W92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2003
Last modified: February 17, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.