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Protein

Protein VAC14 homolog

Gene

Vac14

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 5-phosphate (PtdIns5P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes (By similarity).By similarity

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-RNO-1660514 Synthesis of PIPs at the Golgi membrane
R-RNO-1660516 Synthesis of PIPs at the early endosome membrane
R-RNO-1660517 Synthesis of PIPs at the late endosome membrane

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VAC14 homolog
Gene namesi
Name:Vac14
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi631410 Vac14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi781 – 783VVL → AAA: Reduced interaction with NOS1. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003004871 – 783Protein VAC14 homologAdd BLAST783

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei11PhosphothreonineBy similarity1
Modified residuei500PhosphothreonineBy similarity1
Modified residuei518PhosphoserineBy similarity1
Modified residuei744PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ80W92
PRIDEiQ80W92

PTM databases

iPTMnetiQ80W92
PhosphoSitePlusiQ80W92

Expressioni

Tissue specificityi

Expressed in brain, lung, kidney and testis with highest levels in brain and testis.1 Publication

Developmental stagei

Expressed in embryonic brain.1 Publication

Gene expression databases

BgeeiENSRNOG00000017219
ExpressionAtlasiQ80W92 baseline and differential
GenevisibleiQ80W92 RN

Interactioni

Subunit structurei

Forms homooligomers. Component of the PI(3,5)P2 regulatory complex/PAS complex, at least composed of PIKFYVE, FIG4 and VAC14. VAC14 nucleates the assembly of the complex and serves as a scaffold (By similarity). Interacts with NOS1.By similarity1 Publication

Protein-protein interaction databases

BioGridi258827, 1 interactor
IntActiQ80W92, 1 interactor
MINTiQ80W92
STRINGi10116.ENSRNOP00000023383

Structurei

3D structure databases

ProteinModelPortaliQ80W92
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati5 – 42HEAT 1Add BLAST38
Repeati89 – 126HEAT 2Add BLAST38
Repeati171 – 208HEAT 3Add BLAST38
Repeati212 – 249HEAT 4Add BLAST38
Repeati439 – 476HEAT 5Add BLAST38
Repeati561 – 599HEAT 6Add BLAST39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni774 – 778Mediates interaction with the PDZ domain of NOS15

Domaini

The C-terminal domain (residues 524-783) mediates homomeric interactions and is necessary for the formation and maintenance of the PI(3,5)P2 regulatory complex.By similarity

Sequence similaritiesi

Belongs to the VAC14 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0212 Eukaryota
ENOG410XP6E LUCA
GeneTreeiENSGT00390000008385
HOGENOMiHOG000216640
HOVERGENiHBG104397
InParanoidiQ80W92
KOiK15305
OMAiCIEKEED
OrthoDBiEOG091G02O6
PhylomeDBiQ80W92
TreeFamiTF343690

Family and domain databases

Gene3Di1.25.10.10, 2 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR026825 Vac14
IPR032878 Vac14_Fab1-bd
IPR021841 VAC14_Fig4p-bd
PANTHERiPTHR16023 PTHR16023, 1 hit
PfamiView protein in Pfam
PF12755 Vac14_Fab1_bd, 1 hit
PF11916 Vac14_Fig4_bd, 1 hit
SUPFAMiSSF48371 SSF48371, 3 hits

Sequencei

Sequence statusi: Complete.

Q80W92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPEKDFAPL TPNIVRALND KLYEKRKVAA LEIEKLVRDF VAQNNTVQIK
60 70 80 90 100
HVIQTLSQEF ALSQHPHSRK GGLIGLAACS IALGKDSGLY LKELIEPVLT
110 120 130 140 150
CFNDADSRLR YYACEALYNI VKVARGAVLP HFNVLFDGLS KLAADPDPNV
160 170 180 190 200
KSGSELLDRL LKDIVTESSK FDLVGFIPLL RERIYSNNQY ARQFIISWIL
210 220 230 240 250
VLVSVPDINL LDYLPEILDG LFQILGDNGK EIRKMCEVVL GEFLKEIKKN
260 270 280 290 300
PSSVKFAEMA NILVIHCQTT DDLIQLTAMC WMREFIQLAG RVMLPYSSGI
310 320 330 340 350
LTAVLPCLAY DDRKKSIKEV ANVCNQSLMK LVTPEDDEPD EPKSVAQKQT
360 370 380 390 400
EPNPEDSLPK QEGTASGGAS GPCDSSFGSG ISVFTSASTD RAPVTLHLDG
410 420 430 440 450
IVQVLNCHLS DTTIGMMTRI AVLKWLYHLY IKTPRKMFRH TDSLFPILLQ
460 470 480 490 500
TLSDESDEVV LKDLEVLAEI ASSPAGQTDD PGAPDGPDLQ VNHSELQVPT
510 520 530 540 550
SGRANLLNPP NTKGLECSPS TPTMNSYFYK FMINLLQTFS SERKLLEARG
560 570 580 590 600
PFIIRQLCLL LNAENIFHSM ADILLREEDL KFASTMVHTL NTILLTSTEL
610 620 630 640 650
FQLRNQLKDL KTLESQNLFC CLYRSWCHNP VTTVSLCFLT QNYRHAYDLI
660 670 680 690 700
QKFGDLEVTV DFLTEVDKLV QLIECPIFTY LRLQLLDVKN NPYLMKALYG
710 720 730 740 750
LLMLLPQSSA FQLLSHRLQC VPNPELLQTE DCLKAAPKSQ KGDSPSIDYT
760 770 780
ELLQHFEKVQ KQHLEVRHQR SGRGDHLDRR VVL
Length:783
Mass (Da):88,068
Last modified:June 1, 2003 - v1
Checksum:i78312BE8D8A83B39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY220476 mRNA Translation: AAO48767.1
RefSeqiNP_808791.1, NM_177930.2
UniGeneiRn.46366

Genome annotation databases

EnsembliENSRNOT00000023383; ENSRNOP00000023383; ENSRNOG00000017219
GeneIDi307842
KEGGirno:307842
UCSCiRGD:631410 rat

Similar proteinsi

Entry informationi

Entry nameiVAC14_RAT
AccessioniPrimary (citable) accession number: Q80W92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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