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Protein

Roundabout homolog 4

Gene

Robo4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Receptor

Keywords - Biological processi

Angiogenesis, Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Roundabout homolog 4
Gene namesi
Name:Robo4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727947. Robo4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
ChainiPRO_000003104238 – 961Roundabout homolog 4Add BLAST924

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi63 ↔ 125PROSITE-ProRule annotation
Disulfide bondi169 ↔ 218PROSITE-ProRule annotation
Glycosylationi211N-linked (GlcNAc...)Sequence analysis1
Glycosylationi257N-linked (GlcNAc...)Sequence analysis1
Glycosylationi371N-linked (GlcNAc...)Sequence analysis1
Glycosylationi400N-linked (GlcNAc...)Sequence analysis1
Glycosylationi407N-linked (GlcNAc...)Sequence analysis1
Glycosylationi691N-linked (GlcNAc...)Sequence analysis1
Glycosylationi723N-linked (GlcNAc...)Sequence analysis1
Glycosylationi772N-linked (GlcNAc...)Sequence analysis1
Glycosylationi793N-linked (GlcNAc...)Sequence analysis1
Modified residuei823PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ80W87.
PRIDEiQ80W87.

PTM databases

iPTMnetiQ80W87.
PhosphoSitePlusiQ80W87.

Interactioni

Subunit structurei

Interacts with SLIT2 and ENAH.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057050.

Structurei

3D structure databases

ProteinModelPortaliQ80W87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 142Ig-like C2-type 1Add BLAST101
Domaini148 – 235Ig-like C2-type 2Add BLAST88
Domaini259 – 356Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST98
Domaini358 – 453Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96

Sequence similaritiesi

Belongs to the immunoglobulin superfamily. ROBO family.Curated
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410INZ5. Eukaryota.
ENOG410YX7X. LUCA.
HOGENOMiHOG000074193.
HOVERGENiHBG061836.
InParanoidiQ80W87.
KOiK06784.
PhylomeDBiQ80W87.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 4 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80W87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQGEELRAA VDSGGMGLLG TKCPLPLLLL FIMGGKALDS PPQILVHPQD
60 70 80 90 100
QLLQGSGPAK MSCRASGQPL PTIRWLLNGQ PLSMATPDLH YLQSDGTLLL
110 120 130 140 150
HRPPTHGRPQ DDQNILSAIL GVYTCEASNR LGTAVSRGAR LSVAVLQEDF
160 170 180 190 200
RIQPRDTVAV VGESLVLECG PPWGYPKPSV SWWKDGKPLV LQPGKRTVSG
210 220 230 240 250
DSLMVARAEK NDTGTYMCMA TNNAGQRESR AARVSIQESP DHKEHLELLA
260 270 280 290 300
VRIQLENVTL LNPEPVKGPK PGPAVWLSWK VSGPAAPAQS YTALFRAQRD
310 320 330 340 350
PRDQGSPWTE VLLDGLLNAK LGGLRWGQDY EFKVRPSSGR ARGPDSNVLL
360 370 380 390 400
LRLPEQVPSA PPQEVTLRPG NGSVFVSWAP PPAENHNGFI RGYQVWSLGN
410 420 430 440 450
ASLPAANWTV VGEQTQLEIA ARMPGSYCVQ VAAVTGAGAG EPSIPVCLLL
460 470 480 490 500
EQAMEQSARD PSKHVSWTLE QLRATLKRPE VIASGAVLLW LLLLGIAVCI
510 520 530 540 550
YRRRKAGVHL GPGLYRYTSE DAILKHRMDH SDSPWLADTW RSTSGSRDLS
560 570 580 590 600
SSSSLSSRLG VDPRDPLDGR RSLISWDPRS PGVPLLPDTS TFYGSLIAEQ
610 620 630 640 650
TSSPPVRPSP QTPAARRLPP KLTGTSSPWA SSDSLCSRRG LCSPRMSLAP
660 670 680 690 700
AEAWKAKKKQ ELHQANSSPL LQGSHPMEIW AWELGSRASK NLSQSPGPNT
710 720 730 740 750
CSPREAPGAV VAWRALGPQL HRNSSELAAR PLPPTPLSLR GAPSHDPQSQ
760 770 780 790 800
CVEKLQAPSS DPLPAAPLSV LNSSRPSSPQ ASFLSVPSPG SSNLSSSSLS
810 820 830 840 850
SLEEEDQDSV LTPEEVALCL ELSDGEETPT NSVSPMPRAP SPPATYGYIS
860 870 880 890 900
IPTSSGLADM GRAGGGVGSE VGNLLCPPRL CPTPTPSEGS LANGWGSASE
910 920 930 940 950
DNVPSARASL VSSSDGSFLA DAHFARALAV AVDSFGFSLE PREADCVFTG
960
MWARPPPLEW T
Length:961
Mass (Da):102,580
Last modified:June 1, 2003 - v1
Checksum:i1D21E7D4B611899F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti70L → P.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY277635 mRNA. Translation: AAP32918.1.
RefSeqiNP_852040.1. NM_181375.1.
UniGeneiRn.96119.

Genome annotation databases

GeneIDi300518.
KEGGirno:300518.
UCSCiRGD:727947. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY277635 mRNA. Translation: AAP32918.1.
RefSeqiNP_852040.1. NM_181375.1.
UniGeneiRn.96119.

3D structure databases

ProteinModelPortaliQ80W87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057050.

PTM databases

iPTMnetiQ80W87.
PhosphoSitePlusiQ80W87.

Proteomic databases

PaxDbiQ80W87.
PRIDEiQ80W87.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi300518.
KEGGirno:300518.
UCSCiRGD:727947. rat.

Organism-specific databases

CTDi54538.
RGDi727947. Robo4.

Phylogenomic databases

eggNOGiENOG410INZ5. Eukaryota.
ENOG410YX7X. LUCA.
HOGENOMiHOG000074193.
HOVERGENiHBG061836.
InParanoidiQ80W87.
KOiK06784.
PhylomeDBiQ80W87.

Miscellaneous databases

PROiQ80W87.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 4 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49265. SSF49265. 1 hit.
PROSITEiPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiROBO4_RAT
AccessioniPrimary (citable) accession number: Q80W87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.