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Protein

Pleckstrin homology domain-containing family A member 8

Gene

Plekha8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cargo transport protein that is required for apical transport from the trans-Golgi network (TGN). Transports AQP2 from the trans-Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660499. Synthesis of PIPs at the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family A member 8
Short name:
PH domain-containing family A member 8
Alternative name(s):
Phosphatidylinositol-four-phosphate adapter protein 2
Short name:
FAPP-2
Short name:
Phosphoinositol 4-phosphate adapter protein 2
Gene namesi
Name:Plekha8
Synonyms:Fapp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2681164. Plekha8.

Subcellular locationi

  • Golgi apparatustrans-Golgi network membrane By similarity
  • Membrane By similarity; Peripheral membrane protein By similarity

  • Note: Binds through its PH domain to PtdIns4P and ARF1, and subsequently localizes to TGN exit sites.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003068661 – 519Pleckstrin homology domain-containing family A member 8Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139PhosphothreonineBy similarity1
Modified residuei145PhosphoserineBy similarity1
Modified residuei153PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80W71.
PRIDEiQ80W71.

PTM databases

iPTMnetiQ80W71.
PhosphoSitePlusiQ80W71.

Expressioni

Gene expression databases

BgeeiENSMUSG00000005225.
GenevisibleiQ80W71. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with ARF1; the interaction together with phosphatidylinositol 4-phosphate binding is required for FAPP2 GlcCer transfer ability.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112466.

Structurei

3D structure databases

ProteinModelPortaliQ80W71.
SMRiQ80W71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 93PHPROSITE-ProRule annotationAdd BLAST93

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni330 – 473Glycolipid transfer protein homology domainAdd BLAST144

Domaini

The PH domain of FAPPS binds the small GTPase ARF1 and phosphatidylinositol-4-phosphate (PtdIns4P) with high selectivity, and is required for recruitment of FAPPs to the trans-Golgi network (TGN).By similarity

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3221. Eukaryota.
ENOG410YFEA. LUCA.
GeneTreeiENSGT00650000093230.
HOGENOMiHOG000007705.
HOVERGENiHBG108255.
InParanoidiQ80W71.
KOiK08051.
OMAiIVLHEVE.
OrthoDBiEOG091G0IIT.
PhylomeDBiQ80W71.
TreeFamiTF317467.

Family and domain databases

Gene3Di1.10.3520.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR014830. Glycolipid_transfer_prot_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF08718. GLTP. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF110004. SSF110004. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80W71-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGVLYKWTN YLSGWQPRWF LLCGGILSYY DSPEDAWKGC KGSIQMAVCE
60 70 80 90 100
IQVHSVDNTR MDLIIPGEQY FYLKARSVAE RQRWLVALGS AKACLTDSRT
110 120 130 140 150
QKEKEFAENT ENLKTKMSEL RLYCDLLVQQ VDKTKEVATA GVTDSEEGID
160 170 180 190 200
VGTLLKSTCN TFLKTLEECM QIANAAFTSE LLYHTPPGSP QLAVLKSSKM
210 220 230 240 250
KHPIIPIHNS LERSMELNSC ENGSLSIEVN GDEEILMKTK SSLYLKSTEV
260 270 280 290 300
DCSISSEENT DDNVTVQGEI MKEDGEENLE SHDKDPAQPG SDSVCSPESP
310 320 330 340 350
WEDNEEVIPT FFSTMNTSFS DIELLEDSGI PTEAFLASCY AVVPVLDKLG
360 370 380 390 400
PTVFAPVKMD LVGNIKKVNQ KYITNKEEFT TLQKIVLHEV EADVAQVRNS
410 420 430 440 450
ATEALLWLKR GLKFLKGFLT EVKNGEKDIQ TALNNAYGKT LRQHHGWVVR
460 470 480 490 500
GVFALALRAA PSYEDFVAAL TIKEGDHQKE AFSAGMQRDL SLYLPAMEKQ
510
LAILDTLYEI HGLESDEVV
Length:519
Mass (Da):57,962
Last modified:October 2, 2007 - v2
Checksum:i9184716C4D22FABF
GO
Isoform 2 (identifier: Q80W71-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Note: No experimental confirmation available.
Show »
Length:474
Mass (Da):52,732
Checksum:i6387E327708375B1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0285471 – 45Missing in isoform 2. 1 PublicationAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC084800 Genomic DNA. No translation available.
BC052360 mRNA. Translation: AAH52360.1.
BB865855 mRNA. No translation available.
CCDSiCCDS39491.1. [Q80W71-2]
CCDS51781.1. [Q80W71-1]
RefSeqiNP_001001335.1. NM_001001335.2. [Q80W71-2]
NP_001157833.1. NM_001164361.1. [Q80W71-1]
UniGeneiMm.274693.
Mm.35416.

Genome annotation databases

EnsembliENSMUST00000101385; ENSMUSP00000098935; ENSMUSG00000005225. [Q80W71-2]
ENSMUST00000119706; ENSMUSP00000112466; ENSMUSG00000005225. [Q80W71-1]
GeneIDi231999.
KEGGimmu:231999.
UCSCiuc009cab.2. mouse. [Q80W71-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC084800 Genomic DNA. No translation available.
BC052360 mRNA. Translation: AAH52360.1.
BB865855 mRNA. No translation available.
CCDSiCCDS39491.1. [Q80W71-2]
CCDS51781.1. [Q80W71-1]
RefSeqiNP_001001335.1. NM_001001335.2. [Q80W71-2]
NP_001157833.1. NM_001164361.1. [Q80W71-1]
UniGeneiMm.274693.
Mm.35416.

3D structure databases

ProteinModelPortaliQ80W71.
SMRiQ80W71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112466.

PTM databases

iPTMnetiQ80W71.
PhosphoSitePlusiQ80W71.

Proteomic databases

PaxDbiQ80W71.
PRIDEiQ80W71.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000101385; ENSMUSP00000098935; ENSMUSG00000005225. [Q80W71-2]
ENSMUST00000119706; ENSMUSP00000112466; ENSMUSG00000005225. [Q80W71-1]
GeneIDi231999.
KEGGimmu:231999.
UCSCiuc009cab.2. mouse. [Q80W71-1]

Organism-specific databases

CTDi84725.
MGIiMGI:2681164. Plekha8.

Phylogenomic databases

eggNOGiKOG3221. Eukaryota.
ENOG410YFEA. LUCA.
GeneTreeiENSGT00650000093230.
HOGENOMiHOG000007705.
HOVERGENiHBG108255.
InParanoidiQ80W71.
KOiK08051.
OMAiIVLHEVE.
OrthoDBiEOG091G0IIT.
PhylomeDBiQ80W71.
TreeFamiTF317467.

Enzyme and pathway databases

ReactomeiR-MMU-1660499. Synthesis of PIPs at the plasma membrane.

Miscellaneous databases

PROiQ80W71.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005225.
GenevisibleiQ80W71. MM.

Family and domain databases

Gene3Di1.10.3520.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR014830. Glycolipid_transfer_prot_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF08718. GLTP. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF110004. SSF110004. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKHA8_MOUSE
AccessioniPrimary (citable) accession number: Q80W71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: November 30, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.