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Protein

Pleckstrin homology domain-containing family A member 8

Gene

Plekha8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cargo transport protein that is required for apical transport from the trans-Golgi network (TGN). Transports AQP2 from the trans-Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processLipid transport, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660499. Synthesis of PIPs at the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family A member 8
Short name:
PH domain-containing family A member 8
Alternative name(s):
Phosphatidylinositol-four-phosphate adapter protein 2
Short name:
FAPP-2
Short name:
Phosphoinositol 4-phosphate adapter protein 2
Gene namesi
Name:Plekha8
Synonyms:Fapp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2681164. Plekha8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003068661 – 519Pleckstrin homology domain-containing family A member 8Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139PhosphothreonineBy similarity1
Modified residuei145PhosphoserineBy similarity1
Modified residuei153PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80W71.
PRIDEiQ80W71.

PTM databases

iPTMnetiQ80W71.
PhosphoSitePlusiQ80W71.

Expressioni

Gene expression databases

BgeeiENSMUSG00000005225.
GenevisibleiQ80W71. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with ARF1; the interaction together with phosphatidylinositol 4-phosphate binding is required for FAPP2 GlcCer transfer ability.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112466.

Structurei

3D structure databases

ProteinModelPortaliQ80W71.
SMRiQ80W71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 93PHPROSITE-ProRule annotationAdd BLAST93

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni330 – 473Glycolipid transfer protein homology domainAdd BLAST144

Domaini

The PH domain of FAPPS binds the small GTPase ARF1 and phosphatidylinositol-4-phosphate (PtdIns4P) with high selectivity, and is required for recruitment of FAPPs to the trans-Golgi network (TGN).By similarity

Phylogenomic databases

eggNOGiKOG3221. Eukaryota.
ENOG410YFEA. LUCA.
GeneTreeiENSGT00650000093230.
HOGENOMiHOG000007705.
HOVERGENiHBG108255.
InParanoidiQ80W71.
KOiK08051.
OMAiIVLHEVE.
OrthoDBiEOG091G0IIT.
PhylomeDBiQ80W71.
TreeFamiTF317467.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR036497. GLTP_sf.
IPR014830. Glycolipid_transfer_prot_dom.
IPR011993. PH-like_dom_sf.
IPR001849. PH_domain.
PfamiView protein in Pfam
PF08718. GLTP. 1 hit.
PF00169. PH. 1 hit.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SUPFAMiSSF110004. SSF110004. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiView protein in PROSITE
PS50003. PH_DOMAIN. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80W71-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGVLYKWTN YLSGWQPRWF LLCGGILSYY DSPEDAWKGC KGSIQMAVCE
60 70 80 90 100
IQVHSVDNTR MDLIIPGEQY FYLKARSVAE RQRWLVALGS AKACLTDSRT
110 120 130 140 150
QKEKEFAENT ENLKTKMSEL RLYCDLLVQQ VDKTKEVATA GVTDSEEGID
160 170 180 190 200
VGTLLKSTCN TFLKTLEECM QIANAAFTSE LLYHTPPGSP QLAVLKSSKM
210 220 230 240 250
KHPIIPIHNS LERSMELNSC ENGSLSIEVN GDEEILMKTK SSLYLKSTEV
260 270 280 290 300
DCSISSEENT DDNVTVQGEI MKEDGEENLE SHDKDPAQPG SDSVCSPESP
310 320 330 340 350
WEDNEEVIPT FFSTMNTSFS DIELLEDSGI PTEAFLASCY AVVPVLDKLG
360 370 380 390 400
PTVFAPVKMD LVGNIKKVNQ KYITNKEEFT TLQKIVLHEV EADVAQVRNS
410 420 430 440 450
ATEALLWLKR GLKFLKGFLT EVKNGEKDIQ TALNNAYGKT LRQHHGWVVR
460 470 480 490 500
GVFALALRAA PSYEDFVAAL TIKEGDHQKE AFSAGMQRDL SLYLPAMEKQ
510
LAILDTLYEI HGLESDEVV
Length:519
Mass (Da):57,962
Last modified:October 2, 2007 - v2
Checksum:i9184716C4D22FABF
GO
Isoform 2 (identifier: Q80W71-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Note: No experimental confirmation available.
Show »
Length:474
Mass (Da):52,732
Checksum:i6387E327708375B1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0285471 – 45Missing in isoform 2. 1 PublicationAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC084800 Genomic DNA. No translation available.
BC052360 mRNA. Translation: AAH52360.1.
BB865855 mRNA. No translation available.
CCDSiCCDS39491.1. [Q80W71-2]
CCDS51781.1. [Q80W71-1]
RefSeqiNP_001001335.1. NM_001001335.2. [Q80W71-2]
NP_001157833.1. NM_001164361.1. [Q80W71-1]
UniGeneiMm.274693.
Mm.35416.

Genome annotation databases

EnsembliENSMUST00000101385; ENSMUSP00000098935; ENSMUSG00000005225. [Q80W71-2]
ENSMUST00000119706; ENSMUSP00000112466; ENSMUSG00000005225. [Q80W71-1]
GeneIDi231999.
KEGGimmu:231999.
UCSCiuc009cab.2. mouse. [Q80W71-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPKHA8_MOUSE
AccessioniPrimary (citable) accession number: Q80W71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: November 22, 2017
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot