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Protein

ATP-binding cassette sub-family G member 2

Gene

Abcg2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi79 – 868ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: RGD
  • ATP binding Source: UniProtKB-KW
  • drug transmembrane transporter activity Source: RGD
  • protein dimerization activity Source: RGD

GO - Biological processi

  • cellular response to dexamethasone stimulus Source: RGD
  • drug export Source: RGD
  • drug transmembrane transport Source: GOC
  • drug transport Source: RGD
  • embryonic process involved in female pregnancy Source: RGD
  • response to folic acid Source: RGD
  • response to iron ion Source: RGD
  • urate metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-917937. Iron uptake and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family G member 2
Alternative name(s):
Breast cancer resistance protein 1 homolog
Urate exporter
CD_antigen: CD338
Gene namesi
Name:Abcg2
Synonyms:Bcrp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi631345. Abcg2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 395395CytoplasmicSequence analysisAdd
BLAST
Transmembranei396 – 41621HelicalSequence analysisAdd
BLAST
Topological domaini417 – 42812ExtracellularSequence analysisAdd
BLAST
Transmembranei429 – 44921HelicalSequence analysisAdd
BLAST
Topological domaini450 – 47728CytoplasmicSequence analysisAdd
BLAST
Transmembranei478 – 49821HelicalSequence analysisAdd
BLAST
Topological domaini499 – 5068ExtracellularSequence analysis
Transmembranei507 – 52721HelicalSequence analysisAdd
BLAST
Topological domaini528 – 5358CytoplasmicSequence analysis
Transmembranei536 – 55621HelicalSequence analysisAdd
BLAST
Topological domaini557 – 63276ExtracellularSequence analysisAdd
BLAST
Transmembranei633 – 65321HelicalSequence analysisAdd
BLAST
Topological domaini654 – 6574CytoplasmicSequence analysis

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: RGD
  • mitochondrial membrane Source: UniProtKB-SubCell
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Mitochondrion

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3509585.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657ATP-binding cassette sub-family G member 2PRO_0000093390Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi592 ↔ 610By similarity
Glycosylationi596 – 5961N-linked (GlcNAc...)Sequence analysis
Glycosylationi600 – 6001N-linked (GlcNAc...)Sequence analysis
Disulfide bondi603 – 603InterchainBy similarity

Post-translational modificationi

N-glycosylated in brain capillary, kidney and small intestine but not in heart.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ80W57.
PRIDEiQ80W57.

PTM databases

iPTMnetiQ80W57.

Expressioni

Tissue specificityi

Highly expressed in brain capillary, kidney and small intestine. Lower expression in heart. Preferentially expressed (at protein level) on the luminal membrane of brain capillaries, in kidney and small intestine.1 Publication

Gene expression databases

GenevisibleiQ80W57. RN.

Interactioni

Subunit structurei

Monomer under reducing conditions, the minimal functional unit is a homodimer; disulfide-linked, but the major oligomeric form in plasma membranes is a homotetramer with possibility of higher order oligomerization up to homododecamers.By similarity

GO - Molecular functioni

  • protein dimerization activity Source: RGD

Protein-protein interaction databases

MINTiMINT-4997601.
STRINGi10116.ENSRNOP00000009546.

Structurei

3D structure databases

ProteinModelPortaliQ80W57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 285238ABC transporterPROSITE-ProRule annotationAdd
BLAST
Domaini389 – 653265ABC transmembrane type-2Add
BLAST

Domaini

The extracellular loop 3 (ECL3) is involved in binding porphyrins and transfer them to other carriers, probably albumin.By similarity

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IN8P. Eukaryota.
KOG0061. Eukaryota.
COG0842. LUCA.
COG1131. LUCA.
GeneTreeiENSGT00740000114855.
HOGENOMiHOG000046080.
HOVERGENiHBG050441.
InParanoidiQ80W57.
KOiK05681.
OMAiMSSSNDH.
OrthoDBiEOG7HXCR2.
PhylomeDBiQ80W57.
TreeFamiTF105211.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR030256. ABCG2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR19241:SF209. PTHR19241:SF209. 1 hit.
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80W57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSNDHVLV PMSQRNKNGL PGMSSRGART LAEGDVLSFH HITYRVKVKS
60 70 80 90 100
GFLVRKTAEK EILSDINGIM KPGLNAILGP TGGGKSSLLD VLAARKDPRG
110 120 130 140 150
LSGDVLINGA PQPANFKCSS GYVVQDDVVM GTLTVRENLQ FSAALRLPKA
160 170 180 190 200
MKTHEKNERI NTIIKELGLD KVADSKVGTQ FTRGISGGER KRTSIGMELI
210 220 230 240 250
TDPSILFLDE PTTGLDSSTA NAVLLLLKRM SKQGRTIIFS IHQPRYSIFK
260 270 280 290 300
LFDSLTLLAS GKLMFHGPAQ KALEYFASAG YHCEPYNNPA DFFLDVINGD
310 320 330 340 350
SSAVMLNRGE QDHEANKTEE PSKREKPIIE NLAEFYINST IYGETKAELD
360 370 380 390 400
QLPVAQKKKG SSAFREPVYV TSFCHQLRWI ARRSFKNLLG NPQASVAQLI
410 420 430 440 450
VTVILGLIIG ALYFGLKNDP TGMQNRAGVF FFLTTNQCFT SVSAVELFVV
460 470 480 490 500
EKKLFIHEYI SGYYRVSSYF FGKLVSDLLP MRFLPSVIYT CILYFMLGLK
510 520 530 540 550
RTVEAFFIMM FTLIMVAYTA SSMALAIAAG QSVVSVATLL MTISFVFMML
560 570 580 590 600
FSGLLVNLRT IGPWLSWLQY FSIPRYGFTA LQHNEFLGQE FCPGLNVTMN
610 620 630 640 650
STCVNSYTIC TGNDYLINQG IDLSPWGLWR NHVALACMII IFLTIAYLKL

LFLKKYS
Length:657
Mass (Da):72,961
Last modified:June 1, 2003 - v1
Checksum:iC975C61A08489027
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti363 – 3653AFR → PFK in BAC75666 (PubMed:12819005).Curated
Sequence conflicti431 – 4311F → L in BAC75666 (PubMed:12819005).Curated
Sequence conflicti492 – 4921I → L in AAM09106 (Ref. 3) Curated
Sequence conflicti492 – 4921I → L in AAM09107 (Ref. 3) Curated
Sequence conflicti492 – 4921I → L in AAM09108 (Ref. 3) Curated
Sequence conflicti502 – 5021T → L in BAC75666 (PubMed:12819005).Curated
Sequence conflicti510 – 5101M → R in BAC75666 (PubMed:12819005).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB094089 mRNA. Translation: BAC75666.1.
AB105817 mRNA. Translation: BAC76396.1.
AY089996 mRNA. Translation: AAM09106.1.
AY089997 mRNA. Translation: AAM09107.1.
AY089998 mRNA. Translation: AAM09108.1.
AY274118 mRNA. Translation: AAP23237.1.
RefSeqiNP_852046.1. NM_181381.2.
XP_006236634.1. XM_006236572.2.
XP_006236635.1. XM_006236573.2.
XP_006236636.1. XM_006236574.2.
XP_006236637.1. XM_006236575.2.
XP_006236638.1. XM_006236576.1.
XP_008761183.1. XM_008762961.1.
UniGeneiRn.13131.

Genome annotation databases

EnsembliENSRNOT00000009546; ENSRNOP00000009546; ENSRNOG00000007041.
ENSRNOT00000088983; ENSRNOP00000075138; ENSRNOG00000007041.
GeneIDi312382.
KEGGirno:312382.
UCSCiRGD:631345. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB094089 mRNA. Translation: BAC75666.1.
AB105817 mRNA. Translation: BAC76396.1.
AY089996 mRNA. Translation: AAM09106.1.
AY089997 mRNA. Translation: AAM09107.1.
AY089998 mRNA. Translation: AAM09108.1.
AY274118 mRNA. Translation: AAP23237.1.
RefSeqiNP_852046.1. NM_181381.2.
XP_006236634.1. XM_006236572.2.
XP_006236635.1. XM_006236573.2.
XP_006236636.1. XM_006236574.2.
XP_006236637.1. XM_006236575.2.
XP_006236638.1. XM_006236576.1.
XP_008761183.1. XM_008762961.1.
UniGeneiRn.13131.

3D structure databases

ProteinModelPortaliQ80W57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4997601.
STRINGi10116.ENSRNOP00000009546.

Chemistry

ChEMBLiCHEMBL3509585.

PTM databases

iPTMnetiQ80W57.

Proteomic databases

PaxDbiQ80W57.
PRIDEiQ80W57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009546; ENSRNOP00000009546; ENSRNOG00000007041.
ENSRNOT00000088983; ENSRNOP00000075138; ENSRNOG00000007041.
GeneIDi312382.
KEGGirno:312382.
UCSCiRGD:631345. rat.

Organism-specific databases

CTDi9429.
RGDi631345. Abcg2.

Phylogenomic databases

eggNOGiENOG410IN8P. Eukaryota.
KOG0061. Eukaryota.
COG0842. LUCA.
COG1131. LUCA.
GeneTreeiENSGT00740000114855.
HOGENOMiHOG000046080.
HOVERGENiHBG050441.
InParanoidiQ80W57.
KOiK05681.
OMAiMSSSNDH.
OrthoDBiEOG7HXCR2.
PhylomeDBiQ80W57.
TreeFamiTF105211.

Enzyme and pathway databases

ReactomeiR-RNO-917937. Iron uptake and transport.

Miscellaneous databases

PROiQ80W57.

Gene expression databases

GenevisibleiQ80W57. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR030256. ABCG2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR19241:SF209. PTHR19241:SF209. 1 hit.
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Hepatic oval cells have the side population phenotype defined by expression of ATP-binding cassette transporter ABCG2/BCRP1."
    Shimano K., Satake M., Okaya A., Kitanaka J., Kitanaka N., Takemura M., Sakagami M., Terada N., Tsujimura T.
    Am. J. Pathol. 163:3-9(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Functional expression of rat ABCG2 on the luminal side of brain capillaries and its enhancement by astrocyte-derived soluble factor(s)."
    Hori S., Ohtsuki S., Tachikawa M., Kimura N., Kondo T., Watanabe M., Nakashima E., Terasaki T.
    J. Neurochem. 90:526-536(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Brain capillary.
  3. Yabuuchi H., Ishikawa T.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. Zhang W., Stanimirovic D.B.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 506-657.
    Strain: Sprague-Dawley.
    Tissue: Brain endothelium.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiABCG2_RAT
AccessioniPrimary (citable) accession number: Q80W57
Secondary accession number(s): Q80ST1, Q80UR3, Q80XF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.