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Protein

WD repeat domain phosphoinositide-interacting protein 2

Gene

Wipi2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Omegasomes are derived from ER and serve as intermediates for genesis of isolation membrane (also called preautophagosome) from ER cisternae membranes.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat domain phosphoinositide-interacting protein 2
Short name:
WIPI-2
Gene namesi
Name:Wipi2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1923831. Wipi2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000514411 – 445WD repeat domain phosphoinositide-interacting protein 2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei395PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80W47.
MaxQBiQ80W47.
PaxDbiQ80W47.
PeptideAtlasiQ80W47.
PRIDEiQ80W47.

PTM databases

iPTMnetiQ80W47.
PhosphoSitePlusiQ80W47.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029578.
CleanExiMM_WIPI2.
ExpressionAtlasiQ80W47. baseline and differential.
GenevisibleiQ80W47. MM.

Interactioni

Subunit structurei

Interacts with TECPR1 (By similarity). Intercats with ATG16L1.By similarity1 Publication

Protein-protein interaction databases

BioGridi217015. 10 interactors.
IntActiQ80W47. 10 interactors.
STRINGi10090.ENSMUSP00000045201.

Structurei

3D structure databases

ProteinModelPortaliQ80W47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati182 – 222WD 1Sequence analysisAdd BLAST41
Repeati228 – 267WD 2Sequence analysisAdd BLAST40
Repeati311 – 349WD 3Sequence analysisAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi223 – 226FRRG motif1 Publication4

Domaini

The FRRG motif is required for recruitment to PtdIns3P.1 Publication

Sequence similaritiesi

Belongs to the WD repeat SVP1 family.Curated
Contains 3 WD repeats.Sequence analysis

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2110. Eukaryota.
ENOG410XP6Y. LUCA.
GeneTreeiENSGT00730000110845.
HOGENOMiHOG000217544.
HOVERGENiHBG056639.
InParanoidiQ80W47.
KOiK17908.
OMAiCNPQGLC.
OrthoDBiEOG091G08ZS.
PhylomeDBiQ80W47.
TreeFamiTF314879.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR032911. WIPI2.
[Graphical view]
PANTHERiPTHR11227:SF27. PTHR11227:SF27. 2 hits.
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.

Sequencei

Sequence statusi: Complete.

Q80W47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLASQSGEA GAGQLLFANF NQDNTSLAVG SKSGYKFFSL SSVDKLEQIY
60 70 80 90 100
ECTDTEDVCI VERLFSSSLV AIVSLKAPRK LKVCHFKKGT EICNYSYSNT
110 120 130 140 150
ILAVKLNRQR LIVCLEESLY IHNIRDMKVL HTIRETPPNP AGLCALSINN
160 170 180 190 200
DNCYLAYPGS ASIGEVQVFD TINLRAANMI PAHDSPLAAL AFDASGTKLA
210 220 230 240 250
TASEKGTVIR VFSIPEGQKL FEFRRGVKRC VSICSLAFSM DGMFLSASSN
260 270 280 290 300
TETVHIFKLE AVREKPPEEP TTWTGYFGKV LMASTSYLPS QVTEMFNQGR
310 320 330 340 350
AFATVRLPFC GHKNICSLTT IQKIPRLLVG ASDGYLYMYN LDPQEGGECA
360 370 380 390 400
LMRQHRLDGS METTSEIVDS ASHDCPLATQ TYGTAAAKGA YVPSSPTRLG
410 420 430 440
KGQDANLEAY TDDLGAVGGA CLEDEASALR LDEDSEHPPM ILRTD
Length:445
Mass (Da):48,477
Last modified:June 1, 2003 - v1
Checksum:iA4D4FF973B104D93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK155113 mRNA. Translation: BAE33057.1.
AK169051 mRNA. Translation: BAE40839.1.
AK172030 mRNA. Translation: BAE42786.1.
BC044894 mRNA. Translation: AAH44894.1.
CCDSiCCDS19828.1.
RefSeqiNP_848485.1. NM_178398.4.
UniGeneiMm.29432.

Genome annotation databases

EnsembliENSMUST00000036872; ENSMUSP00000045201; ENSMUSG00000029578.
GeneIDi74781.
KEGGimmu:74781.
UCSCiuc009aiy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK155113 mRNA. Translation: BAE33057.1.
AK169051 mRNA. Translation: BAE40839.1.
AK172030 mRNA. Translation: BAE42786.1.
BC044894 mRNA. Translation: AAH44894.1.
CCDSiCCDS19828.1.
RefSeqiNP_848485.1. NM_178398.4.
UniGeneiMm.29432.

3D structure databases

ProteinModelPortaliQ80W47.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217015. 10 interactors.
IntActiQ80W47. 10 interactors.
STRINGi10090.ENSMUSP00000045201.

PTM databases

iPTMnetiQ80W47.
PhosphoSitePlusiQ80W47.

Proteomic databases

EPDiQ80W47.
MaxQBiQ80W47.
PaxDbiQ80W47.
PeptideAtlasiQ80W47.
PRIDEiQ80W47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036872; ENSMUSP00000045201; ENSMUSG00000029578.
GeneIDi74781.
KEGGimmu:74781.
UCSCiuc009aiy.1. mouse.

Organism-specific databases

CTDi26100.
MGIiMGI:1923831. Wipi2.

Phylogenomic databases

eggNOGiKOG2110. Eukaryota.
ENOG410XP6Y. LUCA.
GeneTreeiENSGT00730000110845.
HOGENOMiHOG000217544.
HOVERGENiHBG056639.
InParanoidiQ80W47.
KOiK17908.
OMAiCNPQGLC.
OrthoDBiEOG091G08ZS.
PhylomeDBiQ80W47.
TreeFamiTF314879.

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.

Miscellaneous databases

ChiTaRSiWipi2. mouse.
PROiQ80W47.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029578.
CleanExiMM_WIPI2.
ExpressionAtlasiQ80W47. baseline and differential.
GenevisibleiQ80W47. MM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR032911. WIPI2.
[Graphical view]
PANTHERiPTHR11227:SF27. PTHR11227:SF27. 2 hits.
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiWIPI2_MOUSE
AccessioniPrimary (citable) accession number: Q80W47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.