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Protein

Serine/threonine-protein phosphatase 1 regulatory subunit 10

Gene

Ppp1r10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri854 – 88229C3H1-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 1 regulatory subunit 10
Alternative name(s):
MHC class I region proline-rich protein CAT53
Gene namesi
Name:Ppp1r10
Synonyms:Cat53, Pnuts
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1289273. Ppp1r10.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

  • Note: Found in discrete nucleoplasmic bodies and within nucleoli (By similarity). Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase.By similarity1 Publication

GO - Cellular componenti

  • chromatin Source: UniProtKB
  • chromosome, telomeric region Source: Ensembl
  • nucleoplasm Source: MGI
  • PTW/PP1 phosphatase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi401 – 4011W → A: Loss of interaction with PPP1CA but no effect on interaction with TOX4 or WDR82. Cell cycle arrest at mitosis and cell death. Exhibits normal association with chromososmes but shows defects in the process of chromosome decondensation at late telophase. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 888888Serine/threonine-protein phosphatase 1 regulatory subunit 10PRO_0000071512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei313 – 3131PhosphoserineBy similarity
Modified residuei382 – 3821PhosphoserineBy similarity
Modified residuei398 – 3981PhosphothreonineBy similarity
Modified residuei545 – 5451PhosphoserineBy similarity
Modified residuei591 – 5911PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on Thr-398 by PKA within the region necessary for interaction with PPP1CA.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80W00.
MaxQBiQ80W00.
PaxDbiQ80W00.
PRIDEiQ80W00.

PTM databases

iPTMnetiQ80W00.
PhosphoSiteiQ80W00.

Expressioni

Gene expression databases

BgeeiQ80W00.
CleanExiMM_PPP1R10.
ExpressionAtlasiQ80W00. baseline and differential.
GenevisibleiQ80W00. MM.

Interactioni

Subunit structurei

Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA or PPP1CB or PPP1CC. Interacts with PPP1CC (By similarity). Interacts with PPP1CA, WDR82 and TOX4.By similarity1 Publication

Protein-protein interaction databases

BioGridi206343. 10 interactions.
DIPiDIP-48511N.
IntActiQ80W00. 11 interactions.
MINTiMINT-4117471.
STRINGi10090.ENSMUSP00000084460.

Structurei

3D structure databases

ProteinModelPortaliQ80W00.
SMRiQ80W00. Positions 396-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini73 – 14775TFIIS N-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 348348Interaction with TOX4Add
BLAST
Regioni382 – 45069Essential for PPP1CA inhibitionBy similarityAdd
BLAST
Regioni388 – 41730Necessary for interaction with PPP1CAAdd
BLAST
Regioni393 – 40816Necessary for interaction with PPP1CCBy similarityAdd
BLAST
Regioni418 – 619202Interaction with WDR82Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi420 – 4234PP1-binding motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi304 – 3107Poly-Lys
Compositional biasi540 – 852313Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 C3H1-type zinc finger.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri854 – 88229C3H1-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGTC. Eukaryota.
ENOG410XP4M. LUCA.
GeneTreeiENSGT00530000063820.
HOGENOMiHOG000049285.
HOVERGENiHBG053646.
InParanoidiQ80W00.
KOiK17552.
OMAiGDHGRGH.
OrthoDBiEOG7NSB23.
PhylomeDBiQ80W00.
TreeFamiTF105541.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR003617. TFIIS/CRSP70_N_sub.
IPR017923. TFIIS_N.
IPR000571. Znf_CCCH.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF00642. zf-CCCH. 1 hit.
[Graphical view]
SMARTiSM00509. TFS2N. 1 hit.
SM00356. ZnF_C3H1. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
SSF90229. SSF90229. 1 hit.
PROSITEiPS51319. TFIIS_N. 1 hit.
PS50103. ZF_C3H1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80W00-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSGPIDPKE LLKGLDSFLT RDGEVKSVDG ISKIFSLMKE ARKMVSRCTY
60 70 80 90 100
LNIILQTRAP EVLVKFIDVG GYKLLNNWLT YSKTTNNIPL LQQILLTLQH
110 120 130 140 150
LPLTVDHLKQ NNTAKLVKQL SKSSEDEELR KLASVLVSDW MAVIRSQSST
160 170 180 190 200
QPAEKDKKKR KEEGKSRTTL PERPLTEVKA ETRAEEAPEK KKEKPKSLRT
210 220 230 240 250
TAPSHAKFRS TGLELDTPSL VPVKKNSSTV VVSDKYNLKP IPLKRQSATA
260 270 280 290 300
APGDAAPPAE KKYKPLNTAP NTTKEIKVKI IPPQPMEGLG FLDALNSAPV
310 320 330 340 350
PGIKIKKKKK VLSPTAAKPS PFEGKTSTEQ STAKPSSPEP APPAEPMDTD
360 370 380 390 400
RPGTPVPPVE VPELMDAASS EPGALDAKPV DSPGDPNQLT RKGRKRKTVT
410 420 430 440 450
WPEEGKLREY FYFELDETER VNVNKIKDFG EAAKREILSD RHAFETARRL
460 470 480 490 500
SHDNMEEKVP WVCPRPLVLP SPLVIPGSNS QERYIQAERE KGILQELFLN
510 520 530 540 550
KESPHEPDPE PYEPIPPKLI PLDEECAMDE TPYVETLEPG GSGGSPDGAG
560 570 580 590 600
GSKLPPVLAN LMGSMGAGKS PQGPGGGGIN VQEILTSIMG SPNSHPSEEL
610 620 630 640 650
LKQPDYSDKL KQMLVPHGLL GPGPVANGFP PGGPGGPKGM QHFPPGPGGP
660 670 680 690 700
MPGPHGGPGG PVGPRLLGPP PPSRGGDPFW DGPGDPMRGG PMRGGPGPAP
710 720 730 740 750
GPYHRGRGGR GGNEPPPPPP FRGARGGRSG GGPPNGRGGP GGGGMVGGGG
760 770 780 790 800
HRPHEGPGGS MGSGHRSHDG PGGNMGSGHR SHDGPGGNMG GSGGHRSHEG
810 820 830 840 850
PGHGGPHGHR PHDVPSHRGH DHRGPPPHEH RGHDGHGGGG HRGHDGGHSH
860 870 880
GGDMSNRPVC RHFMMKGNCR YENNCAFYHP GVNGPPLP
Length:888
Mass (Da):94,372
Last modified:June 1, 2003 - v1
Checksum:iF32889274EF3632D
GO
Isoform 2 (identifier: Q80W00-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     756-769: Missing.

Note: No experimental confirmation available.
Show »
Length:874
Mass (Da):93,051
Checksum:iF6F3A0ED8C7C488B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301G → A in CAD44294 (Ref. 1) Curated
Sequence conflicti85 – 851T → N in CAD44294 (Ref. 1) Curated
Sequence conflicti171 – 1711P → H in CAD44294 (Ref. 1) Curated
Sequence conflicti239 – 2391K → N in CAD44294 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei756 – 76914Missing in isoform 2. 1 PublicationVSP_013155Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ504718 mRNA. Translation: CAD44294.1.
CR974451 Genomic DNA. Translation: CAM27669.1.
BC029765 mRNA. Translation: AAH29765.1.
BC052059 mRNA. Translation: AAH52059.1.
AK053183 mRNA. Translation: BAC35301.1.
CCDSiCCDS50098.1. [Q80W00-1]
RefSeqiNP_001157290.1. NM_001163818.1. [Q80W00-1]
XP_006524679.1. XM_006524616.2. [Q80W00-1]
XP_006524680.1. XM_006524617.2. [Q80W00-1]
XP_006524681.1. XM_006524618.1. [Q80W00-1]
XP_006524682.1. XM_006524619.2. [Q80W00-1]
XP_011239857.1. XM_011241555.1. [Q80W00-1]
XP_011249622.1. XM_011251320.1. [Q80W00-1]
UniGeneiMm.29385.

Genome annotation databases

EnsembliENSMUST00000087210; ENSMUSP00000084460; ENSMUSG00000039220. [Q80W00-1]
ENSMUST00000087211; ENSMUSP00000084461; ENSMUSG00000039220. [Q80W00-1]
GeneIDi52040.
KEGGimmu:52040.
UCSCiuc033hdm.1. mouse. [Q80W00-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ504718 mRNA. Translation: CAD44294.1.
CR974451 Genomic DNA. Translation: CAM27669.1.
BC029765 mRNA. Translation: AAH29765.1.
BC052059 mRNA. Translation: AAH52059.1.
AK053183 mRNA. Translation: BAC35301.1.
CCDSiCCDS50098.1. [Q80W00-1]
RefSeqiNP_001157290.1. NM_001163818.1. [Q80W00-1]
XP_006524679.1. XM_006524616.2. [Q80W00-1]
XP_006524680.1. XM_006524617.2. [Q80W00-1]
XP_006524681.1. XM_006524618.1. [Q80W00-1]
XP_006524682.1. XM_006524619.2. [Q80W00-1]
XP_011239857.1. XM_011241555.1. [Q80W00-1]
XP_011249622.1. XM_011251320.1. [Q80W00-1]
UniGeneiMm.29385.

3D structure databases

ProteinModelPortaliQ80W00.
SMRiQ80W00. Positions 396-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206343. 10 interactions.
DIPiDIP-48511N.
IntActiQ80W00. 11 interactions.
MINTiMINT-4117471.
STRINGi10090.ENSMUSP00000084460.

PTM databases

iPTMnetiQ80W00.
PhosphoSiteiQ80W00.

Proteomic databases

EPDiQ80W00.
MaxQBiQ80W00.
PaxDbiQ80W00.
PRIDEiQ80W00.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087210; ENSMUSP00000084460; ENSMUSG00000039220. [Q80W00-1]
ENSMUST00000087211; ENSMUSP00000084461; ENSMUSG00000039220. [Q80W00-1]
GeneIDi52040.
KEGGimmu:52040.
UCSCiuc033hdm.1. mouse. [Q80W00-1]

Organism-specific databases

CTDi5514.
MGIiMGI:1289273. Ppp1r10.

Phylogenomic databases

eggNOGiENOG410IGTC. Eukaryota.
ENOG410XP4M. LUCA.
GeneTreeiENSGT00530000063820.
HOGENOMiHOG000049285.
HOVERGENiHBG053646.
InParanoidiQ80W00.
KOiK17552.
OMAiGDHGRGH.
OrthoDBiEOG7NSB23.
PhylomeDBiQ80W00.
TreeFamiTF105541.

Miscellaneous databases

ChiTaRSiPpp1r10. mouse.
NextBioi308416.
PROiQ80W00.
SOURCEiSearch...

Gene expression databases

BgeeiQ80W00.
CleanExiMM_PPP1R10.
ExpressionAtlasiQ80W00. baseline and differential.
GenevisibleiQ80W00. MM.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR003617. TFIIS/CRSP70_N_sub.
IPR017923. TFIIS_N.
IPR000571. Znf_CCCH.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF00642. zf-CCCH. 1 hit.
[Graphical view]
SMARTiSM00509. TFS2N. 1 hit.
SM00356. ZnF_C3H1. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
SSF90229. SSF90229. 1 hit.
PROSITEiPS51319. TFIIS_N. 1 hit.
PS50103. ZF_C3H1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genes from major histocompatibility complex (MHC) class I region from HLA-C to HLA-A."
    Raha-Chowdhury R., Andrews S.R., Gruen J.R., Weissman S.M.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J and FVB/N.
    Tissue: Brain and Mammary cancer.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-273.
    Strain: C57BL/6J.
    Tissue: Lung.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Spleen and Testis.
  6. "Identification and characterization of a novel human PP1 phosphatase complex."
    Lee J.H., You J., Dobrota E., Skalnik D.G.
    J. Biol. Chem. 285:24466-24476(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PTW/PP1 PHOSPHATASE COMPLEX, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PPP1CA; TOX4 AND WDR82, MUTAGENESIS OF TRP-401.

Entry informationi

Entry nameiPP1RA_MOUSE
AccessioniPrimary (citable) accession number: Q80W00
Secondary accession number(s): B1B179
, Q811B6, Q8C6T7, Q8K2U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.