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Q80VY9

- DHX33_MOUSE

UniProt

Q80VY9 - DHX33_MOUSE

Protein

Putative ATP-dependent RNA helicase DHX33

Gene

Dhx33

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment. Important element of nucleolar organization By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi88 – 958ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. activating transcription factor binding Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. ATP-dependent helicase activity Source: InterPro
    4. rDNA binding Source: UniProtKB

    GO - Biological processi

    1. positive regulation of transcription from RNA polymerase I promoter Source: UniProtKB

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative ATP-dependent RNA helicase DHX33 (EC:3.6.4.13)
    Alternative name(s):
    DEAH box protein 33
    Gene namesi
    Name:Dhx33
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:2445102. Dhx33.

    Subcellular locationi

    Nucleusnucleolus 1 Publication
    Note: During mitosis, localizes with the nucleolar organizing regions.By similarity

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB
    2. nucleoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi94 – 941K → N or R: Loss of stimulation of rRNA synthesis. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 698698Putative ATP-dependent RNA helicase DHX33PRO_0000055165Add
    BLAST

    Proteomic databases

    PRIDEiQ80VY9.

    PTM databases

    PhosphoSiteiQ80VY9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ80VY9.
    BgeeiQ80VY9.
    CleanExiMM_DHX33.
    GenevestigatoriQ80VY9.

    Interactioni

    Subunit structurei

    Interacts with UBTF.1 Publication

    Protein-protein interaction databases

    BioGridi229816. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80VY9.
    SMRiQ80VY9. Positions 62-689.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini75 – 243169Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini271 – 441171Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi185 – 1884DEAH box
    Motifi536 – 55015Critical for rDNA-bindingAdd
    BLAST

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1643.
    GeneTreeiENSGT00750000117594.
    HOGENOMiHOG000175261.
    HOVERGENiHBG039428.
    InParanoidiQ80VY9.
    KOiK17820.
    OMAiGDMESVR.
    OrthoDBiEOG718KC0.
    PhylomeDBiQ80VY9.
    TreeFamiTF354245.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80VY9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPEEASLPPA KRFRPGSCPP GRRVVMLLTA GGGGGAGGGR RQTPPLAQPS    50
    ASPYREALEL QRRSLPIFRA RGQLLAQLRN LDNAVLIGET GSGKTTQIPQ 100
    YLYEGGISRQ GIIAVTQPRR VAAISLATRV SDEKRTELGK LVGYTVRFED 150
    VTSEDTRIKF LTDGMLLREA ISDSLLRKYS CVILDEAHER TIHTDVLFGV 200
    VKTAQKRRKE LGKLPLKVIV MSATMDVDLF SQYFNRAPVL YLEGRQHPIQ 250
    IFYTKQPQQD YLHAALVSVF QIHQEAPASQ DILVFLTGQE EIEAMSKTCR 300
    DIARHLPDGC PSMLVLPLYA SLPYSQQLRV FQGAPKGYRK VIISTNIAET 350
    SITITGIKYV VDTGMVKAKK YNPDSGLEVL AVQRVSKTQA WQRTGRAGRE 400
    DSGICYRLYT EDEFEKFEKM TVPEIQRCNL ASVILQLLAM KVPNVLTFDF 450
    MSKPSPDHIE AAIAQLDLLG ALEHKDDQLT LTPIGRKMAA FPLEPRFAKT 500
    ILLSSKFHCT EEILTIVSLL SVDSVLYNPP ARRDEVQSVR KKFISSEGDH 550
    ITLLNIYRTF KNIGGNKDWC KENFVNSKNM LLVAEVRAQL REICLKMSMP 600
    IMSSRGDMES VRRCMAHSLF MNTAELQTDG TYATTDTHQP VAIHPSSVLF 650
    HCKPACVVYT SLLYTNKCYM RDLCVVDAEW LYEAAPDYFR RKLRTARN 698
    Length:698
    Mass (Da):78,347
    Last modified:June 1, 2003 - v1
    Checksum:i7DFF1F220C69544E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti470 – 4701G → V in BAC28969. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK035177 mRNA. Translation: BAC28969.1.
    BC052172 mRNA. Translation: AAH52172.1.
    CCDSiCCDS24971.1.
    RefSeqiNP_848144.3. NM_178367.4.
    UniGeneiMm.35399.

    Genome annotation databases

    EnsembliENSMUST00000108527; ENSMUSP00000104167; ENSMUSG00000040620.
    GeneIDi216877.
    KEGGimmu:216877.
    UCSCiuc007jxg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK035177 mRNA. Translation: BAC28969.1 .
    BC052172 mRNA. Translation: AAH52172.1 .
    CCDSi CCDS24971.1.
    RefSeqi NP_848144.3. NM_178367.4.
    UniGenei Mm.35399.

    3D structure databases

    ProteinModelPortali Q80VY9.
    SMRi Q80VY9. Positions 62-689.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229816. 1 interaction.

    PTM databases

    PhosphoSitei Q80VY9.

    Proteomic databases

    PRIDEi Q80VY9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000108527 ; ENSMUSP00000104167 ; ENSMUSG00000040620 .
    GeneIDi 216877.
    KEGGi mmu:216877.
    UCSCi uc007jxg.1. mouse.

    Organism-specific databases

    CTDi 56919.
    MGIi MGI:2445102. Dhx33.

    Phylogenomic databases

    eggNOGi COG1643.
    GeneTreei ENSGT00750000117594.
    HOGENOMi HOG000175261.
    HOVERGENi HBG039428.
    InParanoidi Q80VY9.
    KOi K17820.
    OMAi GDMESVR.
    OrthoDBi EOG718KC0.
    PhylomeDBi Q80VY9.
    TreeFami TF354245.

    Miscellaneous databases

    ChiTaRSi DHX33. mouse.
    NextBioi 375442.
    PROi Q80VY9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q80VY9.
    Bgeei Q80VY9.
    CleanExi MM_DHX33.
    Genevestigatori Q80VY9.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryonic limb.
    3. "Identification of DHX33 as a mediator of rRNA synthesis and cell growth."
      Zhang Y., Forys J.T., Miceli A.P., Gwinn A.S., Weber J.D.
      Mol. Cell. Biol. 31:4676-4691(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH UBTF, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-94.

    Entry informationi

    Entry nameiDHX33_MOUSE
    AccessioniPrimary (citable) accession number: Q80VY9
    Secondary accession number(s): Q8BS50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3