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Protein

ATP-dependent RNA helicase DHX33

Gene

Dhx33

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Implicated in nucleolar organization, ribosome biogenesis, protein synthesis and cytoplasmic dsRNA sensing (By similarity) (PubMed:21930779). Stimulates RNA polymerase I transcription of the 47S precursor rRNA. Associates with ribosomal DNA (rDNA) loci where it is involved in POLR1A recruitment (PubMed:21930779). In the cytoplasm, promotes elongation-competent 80S ribosome assembly at the late stage of mRNA translation initiation (PubMed:26100019). Senses cytosolic dsRNA mediating NLRP3 inflammasome formation in macrophages and type I interferon production in myeloid dendritic cells (By similarity). Required for NLRP3 activation induced by viral dsRNA and bacterial RNA (By similarity). In dendritic cells, required for induction of type I interferon production induced by cytoplasmic dsRNA via the activation of MAPK and NF-kappa-B signaling pathways (PubMed:24037184).By similarity3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 95ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • activating transcription factor binding Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • double-stranded RNA binding Source: UniProtKB
  • mRNA binding Source: UniProtKB
  • rDNA binding Source: UniProtKB
  • ribosomal large subunit binding Source: Ensembl
  • RNA binding Source: GO_Central

GO - Biological processi

  • positive regulation of MAPK cascade Source: UniProtKB
  • positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • positive regulation of NLRP3 inflammasome complex assembly Source: UniProtKB
  • positive regulation of transcription by RNA polymerase I Source: UniProtKB
  • positive regulation of type I interferon production Source: UniProtKB
  • RNA processing Source: GO_Central
  • translational initiation Source: UniProtKB

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DHX33Curated (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 33
Gene namesi
Name:Dhx33Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2445102 Dhx33

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Inflammasome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94K → N or R: Loss of stimulation of rRNA synthesis. Loss of protein synthesis promotion. No effect on interaction with DDX3X, EIF3G, EIF3H and RPL27. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551651 – 698ATP-dependent RNA helicase DHX33Add BLAST698

Post-translational modificationi

Ubiquitinated, leading to its degradation by the proteasome (PubMed:29273634). Deubiquitinated by USP36 (PubMed:29273634).1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ80VY9
MaxQBiQ80VY9
PaxDbiQ80VY9
PeptideAtlasiQ80VY9
PRIDEiQ80VY9

PTM databases

iPTMnetiQ80VY9
PhosphoSitePlusiQ80VY9

Expressioni

Gene expression databases

BgeeiENSMUSG00000040620
CleanExiMM_DHX33
ExpressionAtlasiQ80VY9 baseline and differential
GenevisibleiQ80VY9 MM

Interactioni

Subunit structurei

Interacts with UBTF (PubMed:21930779). Interacts with DDX3X, EIF3G and EIF3H; the interaction is independent of RNA (PubMed:26100019). Interacts (via HA2 region and Helicase C-terminal domain) with the components of the large ribosomal subunit RPL3, RPL7, RPL26 and RPL27 (PubMed:26100019). Binds to mRNA (PubMed:26100019). Interacts (via the helicase C-terminal domain) with MAVS (PubMed:24037184). Binds to double-stranded RNA (via the helicase C-terminal domain) (PubMed:24037184).3 Publications

GO - Molecular functioni

  • activating transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi229816, 1 interactor
STRINGi10090.ENSMUSP00000104167

Structurei

3D structure databases

ProteinModelPortaliQ80VY9
SMRiQ80VY9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini75 – 243Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini271 – 441Helicase C-terminalPROSITE-ProRule annotationAdd BLAST171

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 71Required for nucleolar locationBy similarityAdd BLAST71
Regioni462 – 553HA2; required for interaction with EIF3G and RPL26By similarityAdd BLAST92

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi185 – 188DEAH box4
Motifi536 – 550Critical for rDNA-bindingAdd BLAST15

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0922 Eukaryota
COG1643 LUCA
GeneTreeiENSGT00910000144017
HOGENOMiHOG000175261
HOVERGENiHBG039428
InParanoidiQ80VY9
KOiK17820
OMAiMDIDHFG
OrthoDBiEOG091G030Q
PhylomeDBiQ80VY9
TreeFamiTF354245

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q80VY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEEASLPPA KRFRPGSCPP GRRVVMLLTA GGGGGAGGGR RQTPPLAQPS
60 70 80 90 100
ASPYREALEL QRRSLPIFRA RGQLLAQLRN LDNAVLIGET GSGKTTQIPQ
110 120 130 140 150
YLYEGGISRQ GIIAVTQPRR VAAISLATRV SDEKRTELGK LVGYTVRFED
160 170 180 190 200
VTSEDTRIKF LTDGMLLREA ISDSLLRKYS CVILDEAHER TIHTDVLFGV
210 220 230 240 250
VKTAQKRRKE LGKLPLKVIV MSATMDVDLF SQYFNRAPVL YLEGRQHPIQ
260 270 280 290 300
IFYTKQPQQD YLHAALVSVF QIHQEAPASQ DILVFLTGQE EIEAMSKTCR
310 320 330 340 350
DIARHLPDGC PSMLVLPLYA SLPYSQQLRV FQGAPKGYRK VIISTNIAET
360 370 380 390 400
SITITGIKYV VDTGMVKAKK YNPDSGLEVL AVQRVSKTQA WQRTGRAGRE
410 420 430 440 450
DSGICYRLYT EDEFEKFEKM TVPEIQRCNL ASVILQLLAM KVPNVLTFDF
460 470 480 490 500
MSKPSPDHIE AAIAQLDLLG ALEHKDDQLT LTPIGRKMAA FPLEPRFAKT
510 520 530 540 550
ILLSSKFHCT EEILTIVSLL SVDSVLYNPP ARRDEVQSVR KKFISSEGDH
560 570 580 590 600
ITLLNIYRTF KNIGGNKDWC KENFVNSKNM LLVAEVRAQL REICLKMSMP
610 620 630 640 650
IMSSRGDMES VRRCMAHSLF MNTAELQTDG TYATTDTHQP VAIHPSSVLF
660 670 680 690
HCKPACVVYT SLLYTNKCYM RDLCVVDAEW LYEAAPDYFR RKLRTARN
Length:698
Mass (Da):78,347
Last modified:June 1, 2003 - v1
Checksum:i7DFF1F220C69544E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti470G → V in BAC28969 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK035177 mRNA Translation: BAC28969.1
BC052172 mRNA Translation: AAH52172.1
CCDSiCCDS24971.1
RefSeqiNP_848144.3, NM_178367.4
UniGeneiMm.35399

Genome annotation databases

EnsembliENSMUST00000108527; ENSMUSP00000104167; ENSMUSG00000040620
GeneIDi216877
KEGGimmu:216877
UCSCiuc007jxg.1 mouse

Similar proteinsi

Entry informationi

Entry nameiDHX33_MOUSE
AccessioniPrimary (citable) accession number: Q80VY9
Secondary accession number(s): Q8BS50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: June 1, 2003
Last modified: April 25, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health