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Protein

RNA polymerase II elongation factor ELL3

Gene

Ell3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Enhancer-binding elongation factor that specifically binds enhancers in embryonic stem cells (ES cells), marks them, and is required for their future activation during stem cell specification. Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Does not only bind to enhancer regions of active genes, but also marks the enhancers that are in a poised or inactive state in ES cells and is required for establishing proper RNA polymerase II occupancy at developmentally regulated genes in a cohesin-dependent manner. Probably required for priming developmentally regulated genes for later recruitment of the super elongation complex (SEC), for transcriptional activation during differentiation. Required for recruitment of P-TEFb within SEC during differentiation. Probably preloaded on germ cell chromatin, suggesting that it may prime gene activation by marking enhancers as early as in the germ cells. Promoting epithelial-mesenchymal transition (EMT).2 Publications

GO - Molecular functioni

  • enhancer binding Source: UniProtKB

GO - Biological processi

  • DNA-templated transcription, elongation Source: MGI
  • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: MGI
  • negative regulation of signal transduction by p53 class mediator Source: MGI
  • positive regulation of DNA-templated transcription, elongation Source: MGI
  • positive regulation of neural precursor cell proliferation Source: MGI
  • positive regulation of neurogenesis Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of epithelial to mesenchymal transition Source: UniProtKB
  • snRNA transcription from RNA polymerase II promoter Source: UniProtKB
  • stem cell differentiation Source: UniProtKB
  • transcription elongation from RNA polymerase II promoter Source: MGI
  • transcription from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II elongation factor ELL3
Gene namesi
Name:Ell3
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2673679. Ell3.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • transcription elongation factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395RNA polymerase II elongation factor ELL3PRO_0000146737Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei242 – 2421PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80VR2.
PeptideAtlasiQ80VR2.
PRIDEiQ80VR2.

Expressioni

Tissue specificityi

Actively expressed in embryonic stem cells (ES cells), while it is weakly expressed in differentiated cells.1 Publication

Gene expression databases

BgeeiQ80VR2.
CleanExiMM_ELL3.
GenevisibleiQ80VR2. MM.

Interactioni

Subunit structurei

Component of the little elongation complex (LEC), at least composed of ELL (ELL, ELL2 or ELL3), ZC3H8, ICE1 and ICE2 (By similarity). Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with AFF4.By similarity2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028679.

Structurei

3D structure databases

ProteinModelPortaliQ80VR2.
SMRiQ80VR2. Positions 285-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ELL/occludin family.Curated

Phylogenomic databases

eggNOGiKOG4796. Eukaryota.
ENOG410ZNGU. LUCA.
GeneTreeiENSGT00550000074378.
HOGENOMiHOG000231840.
HOVERGENiHBG051462.
InParanoidiQ80VR2.
KOiK15183.
OMAiLHCLGPL.
OrthoDBiEOG7P02HN.
PhylomeDBiQ80VR2.
TreeFamiTF337345.

Family and domain databases

InterProiIPR031176. ELL/occludin.
IPR031175. ELL3.
IPR019464. ELL_N.
IPR010844. Occludin_ELL.
[Graphical view]
PANTHERiPTHR23288. PTHR23288. 1 hit.
PTHR23288:SF18. PTHR23288:SF18. 1 hit.
PfamiPF10390. ELL. 1 hit.
PF07303. Occludin_ELL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80VR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGTQEALSG KMRLLFTPAA RTSLLMLRLN EAALRALQEC QQQQVRPVIA
60 70 80 90 100
FQGHRGYLRF PGPGWSCLFS FIVSQCGQEG TNGGLDLVYQ RLGRSGPNCL
110 120 130 140 150
HCLGSLRERL TIWAAMDTIP APLLAQEHLT EGTRESESWQ DTGDEPEGHP
160 170 180 190 200
QLAPDEVSDP LASHHEQSLP GSSSEPMAQW EMRNHTYLPS REPDQSLLSP
210 220 230 240 250
ASQKRLDKKR SAPITTEEPE EKRLRALPLA SSPLQGLANQ DSQEGEDWGQ
260 270 280 290 300
DEDEEGDEDG DSRLEQSLSA PSASESPSPE EVPDYLLQYR AIHSTEQQQA
310 320 330 340 350
YEQDFETDYA EYRILHARVG AASQRFTELG AEIKRLQRGT PEHKVLEDKI
360 370 380 390
VQEYKKFRKR YPSYREEKHR CEYLHQKLSH IKGLILEFEE KNRGS
Length:395
Mass (Da):44,762
Last modified:June 1, 2003 - v1
Checksum:i9297E95C5AD222EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL845466 Genomic DNA. Translation: CAM24232.1.
BC045151 mRNA. Translation: AAH45151.1.
CCDSiCCDS16644.1.
RefSeqiNP_666085.2. NM_145973.2.
XP_011237877.1. XM_011239575.1.
UniGeneiMm.330522.

Genome annotation databases

EnsembliENSMUST00000028679; ENSMUSP00000028679; ENSMUSG00000027246.
ENSMUST00000116432; ENSMUSP00000112133; ENSMUSG00000027246.
GeneIDi269344.
KEGGimmu:269344.
UCSCiuc008lzc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL845466 Genomic DNA. Translation: CAM24232.1.
BC045151 mRNA. Translation: AAH45151.1.
CCDSiCCDS16644.1.
RefSeqiNP_666085.2. NM_145973.2.
XP_011237877.1. XM_011239575.1.
UniGeneiMm.330522.

3D structure databases

ProteinModelPortaliQ80VR2.
SMRiQ80VR2. Positions 285-393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028679.

Proteomic databases

PaxDbiQ80VR2.
PeptideAtlasiQ80VR2.
PRIDEiQ80VR2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028679; ENSMUSP00000028679; ENSMUSG00000027246.
ENSMUST00000116432; ENSMUSP00000112133; ENSMUSG00000027246.
GeneIDi269344.
KEGGimmu:269344.
UCSCiuc008lzc.1. mouse.

Organism-specific databases

CTDi80237.
MGIiMGI:2673679. Ell3.

Phylogenomic databases

eggNOGiKOG4796. Eukaryota.
ENOG410ZNGU. LUCA.
GeneTreeiENSGT00550000074378.
HOGENOMiHOG000231840.
HOVERGENiHBG051462.
InParanoidiQ80VR2.
KOiK15183.
OMAiLHCLGPL.
OrthoDBiEOG7P02HN.
PhylomeDBiQ80VR2.
TreeFamiTF337345.

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.

Miscellaneous databases

ChiTaRSiEll3. mouse.
PROiQ80VR2.
SOURCEiSearch...

Gene expression databases

BgeeiQ80VR2.
CleanExiMM_ELL3.
GenevisibleiQ80VR2. MM.

Family and domain databases

InterProiIPR031176. ELL/occludin.
IPR031175. ELL3.
IPR019464. ELL_N.
IPR010844. Occludin_ELL.
[Graphical view]
PANTHERiPTHR23288. PTHR23288. 1 hit.
PTHR23288:SF18. PTHR23288:SF18. 1 hit.
PfamiPF10390. ELL. 1 hit.
PF07303. Occludin_ELL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epitheliumImported.
  3. "AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia."
    Lin C., Smith E.R., Takahashi H., Lai K.C., Martin-Brown S., Florens L., Washburn M.P., Conaway J.W., Conaway R.C., Shilatifard A.
    Mol. Cell 37:429-437(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AFF4.
  4. "Ell3 enhances differentiation of mouse embryonic stem cells by regulating epithelial-mesenchymal transition and apoptosis."
    Ahn H.J., Cha Y., Moon S.H., Jung J.E., Park K.S.
    PLoS ONE 7:E40293-E40293(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "The RNA Pol II elongation factor Ell3 marks enhancers in ES cells and primes future gene activation."
    Lin C., Garruss A.S., Luo Z., Guo F., Shilatifard A.
    Cell 152:144-156(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH AFF4.

Entry informationi

Entry nameiELL3_MOUSE
AccessioniPrimary (citable) accession number: Q80VR2
Secondary accession number(s): A2ARQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2003
Last modified: July 6, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.