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Protein

Epsin-1

Gene

Epn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111Phosphatidylinositol lipid headgroupBy similarity
Binding sitei25 – 251Phosphatidylinositol lipid headgroupBy similarity
Binding sitei30 – 301Phosphatidylinositol lipid headgroupBy similarity
Binding sitei63 – 631Phosphatidylinositol lipid headgroupBy similarity
Binding sitei73 – 731Phosphatidylinositol lipid headgroupBy similarity

GO - Molecular functioni

GO - Biological processi

  • embryonic organ development Source: MGI
  • endocytosis Source: UniProtKB-KW
  • female pregnancy Source: MGI
  • in utero embryonic development Source: MGI
  • Notch signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-182971. EGFR downregulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-1
Alternative name(s):
EPS-15-interacting protein 1
Intersectin-EH-binding protein 1
Short name:
Ibp1
Gene namesi
Name:Epn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1333763. Epn1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity
  • Nucleus By similarity
  • Membraneclathrin-coated pit By similarity

  • Note: Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Coated pit, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575Epsin-1PRO_0000074514Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei382 – 3821PhosphoserineBy similarity
Modified residuei418 – 4181PhosphoserineCombined sources
Modified residuei419 – 4191PhosphoserineCombined sources
Modified residuei420 – 4201PhosphothreonineCombined sources
Modified residuei434 – 4341PhosphoserineBy similarity
Modified residuei446 – 4461PhosphoserineBy similarity
Modified residuei453 – 4531PhosphoserineBy similarity
Modified residuei459 – 4591PhosphothreonineCombined sources
Modified residuei463 – 4631PhosphothreonineBy similarity
Modified residuei469 – 4691PhosphothreonineCombined sources
Modified residuei472 – 4721PhosphoserineCombined sources
Modified residuei493 – 4931PhosphothreonineCombined sources

Post-translational modificationi

Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.1 Publication
Ubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ80VP1.
MaxQBiQ80VP1.
PaxDbiQ80VP1.
PeptideAtlasiQ80VP1.
PRIDEiQ80VP1.

PTM databases

iPTMnetiQ80VP1.
PhosphoSiteiQ80VP1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035203.
CleanExiMM_EPN1.
ExpressionAtlasiQ80VP1. baseline and differential.
GenevisibleiQ80VP1. MM.

Interactioni

Subunit structurei

Monomer. Binds clathrin, ZBTB16/ZNF145, REPS2 and EPS15 (By similarity). Binds ubiquitinated proteins (By similarity). Interacts with RALBP1 in a complex also containing NUMB and TFAP2A during interphase and mitosis (By similarity). Interacts with AP2B1 (By similarity). Interacts with UBQLN2 (By similarity). Interacts with ITSN1. Interacts with AP2A1 and AP2A2.By similarity2 Publications

Protein-protein interaction databases

BioGridi199485. 3 interactions.
DIPiDIP-43943N.
IntActiQ80VP1. 3 interactions.
MINTiMINT-4094555.
STRINGi10090.ENSMUSP00000043340.

Structurei

3D structure databases

ProteinModelPortaliQ80VP1.
SMRiQ80VP1. Positions 1-158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 144133ENTHPROSITE-ProRule annotationAdd
BLAST
Domaini183 – 20220UIM 1PROSITE-ProRule annotationAdd
BLAST
Domaini208 – 22720UIM 2PROSITE-ProRule annotationAdd
BLAST
Domaini233 – 25220UIM 3PROSITE-ProRule annotationAdd
BLAST
Repeati274 – 27631
Repeati294 – 29632
Repeati306 – 30833
Repeati319 – 32134
Repeati332 – 33435
Repeati349 – 35136
Repeati367 – 36937
Repeati377 – 37938
Repeati501 – 50331
Repeati517 – 51932
Repeati571 – 57333

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni274 – 3791068 X 3 AA repeats of D-P-WAdd
BLAST
Regioni501 – 573733 X 3 AA repeats of N-P-FAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi401 – 41010[DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi267 – 572306Ala/Gly/Pro-richAdd
BLAST

Domaini

The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP2A2 and clathrin binding.
The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction the AP-2 complex subunit AP2B1.By similarity

Sequence similaritiesi

Belongs to the epsin family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation
Contains 3 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2056. Eukaryota.
ENOG410XSM0. LUCA.
GeneTreeiENSGT00550000074611.
HOGENOMiHOG000008298.
HOVERGENiHBG006690.
InParanoidiQ80VP1.
KOiK12471.
OMAiMSGVRGS.
OrthoDBiEOG091G0L45.
PhylomeDBiQ80VP1.
TreeFamiTF313361.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF01417. ENTH. 1 hit.
PF02809. UIM. 2 hits.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 3 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80VP1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTSSLRRQM KNIVHNYSEA EIKVREATSN DPWGPSSSLM SEIADLTYNV
60 70 80 90 100
VAFSEIMSMI WKRLNDHGKN WRHVYKAMTL MEYLIKTGSE RVSQQCKENM
110 120 130 140 150
YAVQTLKDFQ YVDRDGKDQG VNVREKAKQL VALLRDEDRL REERAHALKT
160 170 180 190 200
KEKLAQTATA SSAAVGSGPP PEAEQAWPQS SGEEELQLQL ALAMSKEEAD
210 220 230 240 250
QPPSCGPEDD VQLQLALSLS REEHDKEERI RRGDDLRLQM AIEESKRETG
260 270 280 290 300
GKEESSLMDL ADVFTTPAPP QASDPWGGPA SVPTAVPVAA AASDPWGGPA
310 320 330 340 350
VPPAADPWGG AAPTPASGDP WRPAAPTGPS VDPWGGTPAP AAGEGPTPDP
360 370 380 390 400
WGSSDGGAPV SGPPSSDPWA PAPAFSDPWG GSPAKPSSNG TAVGGFDTEP
410 420 430 440 450
DEFSDFDRLR TALPTSGSST GELELLAGEV PARSPGAFDM SGVGGSLAES
460 470 480 490 500
VGSPPPAATP TPTPPTRKTP ESFLGPNAAL VDLDSLVSRP GPTPPGSKAS
510 520 530 540 550
NPFLPSGAPP TGPSVTNPFQ PAPPATLTLN QLRLSPVPPV PGAPPTYISP
560 570
LGGGPGLPPM MPPGPPAPNT NPFLL
Length:575
Mass (Da):60,212
Last modified:November 28, 2006 - v3
Checksum:i70B8011EB3AE5C4C
GO
Isoform 2 (identifier: Q80VP1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     392-392: A → AA

Note: No experimental confirmation available.
Show »
Length:576
Mass (Da):60,283
Checksum:i162775BC51E4E88A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei392 – 3921A → AA in isoform 2. 1 PublicationVSP_009153

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC046962 mRNA. Translation: AAH46962.2.
BC067206 mRNA. Translation: AAH67206.1.
BC099682 mRNA. Translation: AAH99682.1.
AF057285 mRNA. Translation: AAC97475.1.
CCDSiCCDS20758.1. [Q80VP1-1]
RefSeqiNP_001239383.1. NM_001252454.1. [Q80VP1-1]
NP_034277.1. NM_010147.4. [Q80VP1-1]
XP_006539592.2. XM_006539529.2. [Q80VP1-2]
XP_006539594.2. XM_006539531.2. [Q80VP1-2]
XP_006539596.1. XM_006539533.1. [Q80VP1-2]
XP_006539597.1. XM_006539534.1. [Q80VP1-2]
XP_006539598.1. XM_006539535.1. [Q80VP1-2]
XP_006539599.1. XM_006539536.1. [Q80VP1-2]
XP_011248736.1. XM_011250434.1. [Q80VP1-2]
UniGeneiMm.3091.

Genome annotation databases

EnsembliENSMUST00000045277; ENSMUSP00000043340; ENSMUSG00000035203. [Q80VP1-1]
ENSMUST00000098845; ENSMUSP00000096445; ENSMUSG00000035203. [Q80VP1-1]
ENSMUST00000208634; ENSMUSP00000146638; ENSMUSG00000035203. [Q80VP1-2]
GeneIDi13854.
KEGGimmu:13854.
UCSCiuc009ezx.2. mouse. [Q80VP1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Protein Spotlight

The bubble's bend - Issue 42 of January 2004

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC046962 mRNA. Translation: AAH46962.2.
BC067206 mRNA. Translation: AAH67206.1.
BC099682 mRNA. Translation: AAH99682.1.
AF057285 mRNA. Translation: AAC97475.1.
CCDSiCCDS20758.1. [Q80VP1-1]
RefSeqiNP_001239383.1. NM_001252454.1. [Q80VP1-1]
NP_034277.1. NM_010147.4. [Q80VP1-1]
XP_006539592.2. XM_006539529.2. [Q80VP1-2]
XP_006539594.2. XM_006539531.2. [Q80VP1-2]
XP_006539596.1. XM_006539533.1. [Q80VP1-2]
XP_006539597.1. XM_006539534.1. [Q80VP1-2]
XP_006539598.1. XM_006539535.1. [Q80VP1-2]
XP_006539599.1. XM_006539536.1. [Q80VP1-2]
XP_011248736.1. XM_011250434.1. [Q80VP1-2]
UniGeneiMm.3091.

3D structure databases

ProteinModelPortaliQ80VP1.
SMRiQ80VP1. Positions 1-158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199485. 3 interactions.
DIPiDIP-43943N.
IntActiQ80VP1. 3 interactions.
MINTiMINT-4094555.
STRINGi10090.ENSMUSP00000043340.

PTM databases

iPTMnetiQ80VP1.
PhosphoSiteiQ80VP1.

Proteomic databases

EPDiQ80VP1.
MaxQBiQ80VP1.
PaxDbiQ80VP1.
PeptideAtlasiQ80VP1.
PRIDEiQ80VP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045277; ENSMUSP00000043340; ENSMUSG00000035203. [Q80VP1-1]
ENSMUST00000098845; ENSMUSP00000096445; ENSMUSG00000035203. [Q80VP1-1]
ENSMUST00000208634; ENSMUSP00000146638; ENSMUSG00000035203. [Q80VP1-2]
GeneIDi13854.
KEGGimmu:13854.
UCSCiuc009ezx.2. mouse. [Q80VP1-1]

Organism-specific databases

CTDi29924.
MGIiMGI:1333763. Epn1.

Phylogenomic databases

eggNOGiKOG2056. Eukaryota.
ENOG410XSM0. LUCA.
GeneTreeiENSGT00550000074611.
HOGENOMiHOG000008298.
HOVERGENiHBG006690.
InParanoidiQ80VP1.
KOiK12471.
OMAiMSGVRGS.
OrthoDBiEOG091G0L45.
PhylomeDBiQ80VP1.
TreeFamiTF313361.

Enzyme and pathway databases

ReactomeiR-MMU-182971. EGFR downregulation.

Miscellaneous databases

PROiQ80VP1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035203.
CleanExiMM_EPN1.
ExpressionAtlasiQ80VP1. baseline and differential.
GenevisibleiQ80VP1. MM.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013809. ENTH.
IPR008942. ENTH_VHS.
IPR003903. UIM_dom.
[Graphical view]
PfamiPF01417. ENTH. 1 hit.
PF02809. UIM. 2 hits.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 3 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEPN1_MOUSE
AccessioniPrimary (citable) accession number: Q80VP1
Secondary accession number(s): O70446, Q6NX78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 28, 2006
Last modified: September 7, 2016
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.