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Protein

E3 ubiquitin-protein ligase TRIM56

Gene

Trim56

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity. In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets TMEM173/STING to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta. Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 60RING-typePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri98 – 149B box-type 1PROSITE-ProRule annotationAdd BLAST52
Zinc fingeri164 – 205B box-type 2PROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • poly(A) RNA binding Source: MGI
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • defense response to virus Source: UniProtKB
  • innate immune response Source: UniProtKB
  • interferon-beta production Source: UniProtKB
  • negative regulation of viral entry into host cell Source: UniProtKB
  • negative regulation of viral release from host cell Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
  • response to type I interferon Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM56 (EC:6.3.2.-)
Alternative name(s):
Tripartite motif-containing protein 56
Gene namesi
Name:Trim56
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2685298. Trim56.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562891 – 734E3 ubiquitin-protein ligase TRIM56Add BLAST734

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei454PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80VI1.
MaxQBiQ80VI1.
PaxDbiQ80VI1.
PeptideAtlasiQ80VI1.
PRIDEiQ80VI1.

PTM databases

iPTMnetiQ80VI1.
PhosphoSitePlusiQ80VI1.

Expressioni

Inductioni

By interferon.1 Publication

Gene expression databases

BgeeiENSMUSG00000043279.
CleanExiMM_TRIM56.

Interactioni

Subunit structurei

Interacts with TMEM173/STING (PubMed:21074459). Interacts with TICAM1 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi239120. 1 interactor.
IntActiQ80VI1. 2 interactors.
MINTiMINT-4138537.
STRINGi10090.ENSMUSP00000058109.

Structurei

3D structure databases

ProteinModelPortaliQ80VI1.
SMRiQ80VI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili215 – 303Sequence analysisAdd BLAST89

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi302 – 309Poly-Ala8
Compositional biasi438 – 443Poly-Lys6

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 2 B box-type zinc fingers.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 60RING-typePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri98 – 149B box-type 1PROSITE-ProRule annotationAdd BLAST52
Zinc fingeri164 – 205B box-type 2PROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITEJ. Eukaryota.
ENOG410YNG0. LUCA.
HOGENOMiHOG000154679.
HOVERGENiHBG079280.
InParanoidiQ80VI1.
KOiK12026.
PhylomeDBiQ80VI1.
TreeFamiTF338323.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
SMARTiSM00336. BBOX. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80VI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSKVSSPTL LEALSSDFLA CKICLEQLHT PKTLPCLHTY CQDCLAQLDI
60 70 80 90 100
GGQVRCPECR EIVPVPAEGV AAFKTNFFVN GLLDLVKARA PGDVHSGKPT
110 120 130 140 150
CALCPLVGGK SSGGPATARC LDCADDLCQA CADGHRCSRQ THKHRVVDLV
160 170 180 190 200
GYRAGWYDEE ARERQASQCP QHPGEALCFL CQPCSQLLCK DCRLGPHIDH
210 220 230 240 250
PCLPLAEAVR SRKPGLEELL AGVDSNLVEL EATRVAEKEA LALLREQAAS
260 270 280 290 300
VGTQVEEAAE RILKSLLAQK QEVLGQLRAL VEAAEEATRE RLTKIERQEQ
310 320 330 340 350
VAKAAAAFAR RVLSLGLEAE ILSLEGAITQ RLRQLQDAPW TSGPTRCVLP
360 370 380 390 400
QLELHPGLED KNCHLLRLIF EEPKQSPKDS GKGGAGTQGG DEAQGQGDDR
410 420 430 440 450
TKIGKQGGAQ PLTPKEGKDQ NPQEDDGVFI ERGNRPNKKK KCKGRGKSVS
460 470 480 490 500
REPSPILRPN LEGSGLLPRP VFSWSFPTRM PGDKRSPRIT GLCPYGPQEI
510 520 530 540 550
LVADEQNRVL KRFSLNGDYK GTVQVPEGCS PCSVAALQNA VAFSANAKLY
560 570 580 590 600
LVSPDGEIQW RRSLSLTQSS HAVAAMPCGD RVAVSVAGHV EVYKKDGSLA
610 620 630 640 650
TRFIPGGKAS RGQRALVFLT TSPQGNFVGS DWQQNSVVFC DGLGQVIWEY
660 670 680 690 700
KGPGLHGCQP GSVSVDKKGY IFLTLREVNK VVILDPKGSL LGDFLTAYHG
710 720 730
LEKPRVTTMV DGKYLVVSLS NGTIHVFRVR FPDS
Length:734
Mass (Da):79,513
Last modified:June 1, 2003 - v1
Checksum:i3A8192AC7E57BB3C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5V → D in BAC29792 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037350 mRNA. Translation: BAC29792.1.
BC045615 mRNA. Translation: AAH45615.1.
CCDSiCCDS19762.1.
RefSeqiNP_958761.1. NM_201373.4.
XP_006504504.1. XM_006504441.3.
XP_011239185.1. XM_011240883.1.
UniGeneiMm.100647.

Genome annotation databases

GeneIDi384309.
KEGGimmu:384309.
UCSCiuc009abq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037350 mRNA. Translation: BAC29792.1.
BC045615 mRNA. Translation: AAH45615.1.
CCDSiCCDS19762.1.
RefSeqiNP_958761.1. NM_201373.4.
XP_006504504.1. XM_006504441.3.
XP_011239185.1. XM_011240883.1.
UniGeneiMm.100647.

3D structure databases

ProteinModelPortaliQ80VI1.
SMRiQ80VI1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi239120. 1 interactor.
IntActiQ80VI1. 2 interactors.
MINTiMINT-4138537.
STRINGi10090.ENSMUSP00000058109.

PTM databases

iPTMnetiQ80VI1.
PhosphoSitePlusiQ80VI1.

Proteomic databases

EPDiQ80VI1.
MaxQBiQ80VI1.
PaxDbiQ80VI1.
PeptideAtlasiQ80VI1.
PRIDEiQ80VI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi384309.
KEGGimmu:384309.
UCSCiuc009abq.2. mouse.

Organism-specific databases

CTDi81844.
MGIiMGI:2685298. Trim56.

Phylogenomic databases

eggNOGiENOG410ITEJ. Eukaryota.
ENOG410YNG0. LUCA.
HOGENOMiHOG000154679.
HOVERGENiHBG079280.
InParanoidiQ80VI1.
KOiK12026.
PhylomeDBiQ80VI1.
TreeFamiTF338323.

Miscellaneous databases

ChiTaRSiTrim56. mouse.
PROiQ80VI1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000043279.
CleanExiMM_TRIM56.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
SMARTiSM00336. BBOX. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRI56_MOUSE
AccessioniPrimary (citable) accession number: Q80VI1
Secondary accession number(s): Q8CAY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.