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Protein

AP-4 complex subunit epsilon-1

Gene

Ap4e1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.By similarity

GO - Biological processi

  • intracellular protein transport Source: MGI
  • vesicle-mediated transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-4 complex subunit epsilon-1
Alternative name(s):
AP-4 adaptor complex subunit epsilon
Adaptor-related protein complex 4 subunit epsilon-1
Epsilon subunit of AP-4
Epsilon-adaptin
Gene namesi
Name:Ap4e1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1336993. Ap4e1.

Subcellular locationi

GO - Cellular componenti

  • coated pit Source: UniProtKB-SubCell
  • membrane coat Source: InterPro
  • trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11221122AP-4 complex subunit epsilon-1CuratedPRO_0000229749Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei851 – 8511PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80V94.
MaxQBiQ80V94.
PaxDbiQ80V94.
PRIDEiQ80V94.

PTM databases

iPTMnetiQ80V94.
PhosphoSiteiQ80V94.

Expressioni

Gene expression databases

BgeeiQ80V94.
CleanExiMM_AP4E1.
ExpressionAtlasiQ80V94. baseline and differential.
GenevisibleiQ80V94. MM.

Interactioni

Subunit structurei

Adaptor protein complex 4 (AP-4) is a heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002063.

Structurei

3D structure databases

ProteinModelPortaliQ80V94.
SMRiQ80V94. Positions 34-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adaptor complexes large subunit family.Sequence analysis

Phylogenomic databases

eggNOGiKOG1062. Eukaryota.
ENOG410XPKK. LUCA.
GeneTreeiENSGT00390000012618.
HOGENOMiHOG000231886.
HOVERGENiHBG050522.
InParanoidiQ80V94.
KOiK12400.
OMAiSYKIWKD.
OrthoDBiEOG769ZHR.
TreeFamiTF332488.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR017109. AP4_complex_esu.
IPR028269. AP4E1_C.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PANTHERiPTHR22780:SF13. PTHR22780:SF13. 2 hits.
PfamiPF01602. Adaptin_N. 1 hit.
PF14807. AP4E_app_platf. 1 hit.
[Graphical view]
PIRSFiPIRSF037097. AP4_complex_epsilon. 1 hit.
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

Q80V94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDMVERTLT ALPGLFLQNQ LGGPAASRAP FFSRLGGLIR GVTALSSKHE
60 70 80 90 100
EEKLIQQELS SLKATVSAPT TTLKTMKECM VRLIYCEMLG YDASFGYIHA
110 120 130 140 150
IKLAQQGNLL EKRVGYLAVS LFLHESHELL LLLVNTVVKD LQSTNLVEVC
160 170 180 190 200
MALTVVSQIF PREMIPAVLP LIEDKLQHSK EIIRRKAVLA LYKFYLIAPN
210 220 230 240 250
QVQHIHTKFR KALCDRDVGV MAASLHIYLR MIKENASGYK DLTESFVTIL
260 270 280 290 300
KQVVGGKLPV EFSYHSVPAP WLQIQLLRIL GLLGKDDERT SELMYDVLDE
310 320 330 340 350
SLRRAELNHN VTYAILFECV HTIYSIYPKS ELLEKAAKCI GKFVLSPKIN
360 370 380 390 400
LKYLGLKALT YVIQQDPSLA LQHQITIIEC LDHPDPIIKR ETLELLYRIT
410 420 430 440 450
NAQNVVVIVQ KMLEYLHQSK EEHIIISLVG RIAELAEKYA PDNVWFIQTM
460 470 480 490 500
NAVFSVGGDV MHPDILSNFL RLLAEGFDDE TEDQQLRLYA VQSYLTLLDM
510 520 530 540 550
ENTFYPQRFL QVMSWVLGEY SYLLDKESPE EVITRLYKLL MSDSISSETK
560 570 580 590 600
AWLFAAVTKL TPQAHSSPLV EKLIQEFTVS LNTCLRQHAF ELKHLHENTE
610 620 630 640 650
LMKSLLQGAQ NCEDIVADAS LSFLDGFVAE GLSQGAAPYK PHHQRQEEQL
660 670 680 690 700
SQEKVLNFEP YGLSFSSSGF TGRQSPAGIS LGSDISGNSA ETGLKETSSL
710 720 730 740 750
KMEGIKKLWG KEGYLPKKES GTGDKPEASH VPAEGATVEN VDQATTRKDQ
760 770 780 790 800
AQGHIPSTEE KEKQLLASSL FVGLGPENTV DLLGKADVVS HKFRRKSKLK
810 820 830 840 850
VAQSDKTPSA PTAPCSALSL GSDVAGGDED GLSAVDRGDG ELSSELFRSE
860 870 880 890 900
SLSGPPSAEK LESVSLPVPS LFADNNMEVF NPPSSSATST VKEETPECRH
910 920 930 940 950
SGLVEICSNE AVSVSSYKVW RDDCLLVIWA VTSKTDSEFT DAQLEIFPVE
960 970 980 990 1000
NFKIIEQPEC SSPVIETERT KSFQYSVQME SPCIEGTLSG FIKYQMMDTH
1010 1020 1030 1040 1050
SVQLEFSMNL PLLDFIRPLK ISTEDFGKLW LSFANDVKQT IKISEPGVAL
1060 1070 1080 1090 1100
TSVLTELQQN LRLRVIDVIG NEGLLACKLL PSTPCVLHCR VHADAVALWF
1110 1120
RSSSSVLSDY LSCHCQKVMQ TS
Length:1,122
Mass (Da):124,845
Last modified:July 27, 2011 - v3
Checksum:iD5B4E518714DBA3D
GO

Sequence cautioni

The sequence AK144636 differs from that shown. Reason: Frameshift at position 303. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti338 – 3381K → Q in AK144636 (PubMed:16141072).Curated
Sequence conflicti379 – 3791E → G in AK144636 (PubMed:16141072).Curated
Sequence conflicti936 – 9361D → E in AK144636 (PubMed:16141072).Curated
Sequence conflicti954 – 9541I → M in AK144636 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144636 mRNA. No translation available.
AL928836, AL928590 Genomic DNA. Translation: CAM18335.1.
AL928590, AL928836 Genomic DNA. Translation: CAM19126.1.
BC042530 mRNA. Translation: AAH42530.1.
CCDSiCCDS38231.1.
RefSeqiNP_780759.2. NM_175550.3.
UniGeneiMm.134045.

Genome annotation databases

EnsembliENSMUST00000002063; ENSMUSP00000002063; ENSMUSG00000001998.
GeneIDi108011.
KEGGimmu:108011.
UCSCiuc008men.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144636 mRNA. No translation available.
AL928836, AL928590 Genomic DNA. Translation: CAM18335.1.
AL928590, AL928836 Genomic DNA. Translation: CAM19126.1.
BC042530 mRNA. Translation: AAH42530.1.
CCDSiCCDS38231.1.
RefSeqiNP_780759.2. NM_175550.3.
UniGeneiMm.134045.

3D structure databases

ProteinModelPortaliQ80V94.
SMRiQ80V94. Positions 34-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002063.

PTM databases

iPTMnetiQ80V94.
PhosphoSiteiQ80V94.

Proteomic databases

EPDiQ80V94.
MaxQBiQ80V94.
PaxDbiQ80V94.
PRIDEiQ80V94.

Protocols and materials databases

DNASUi108011.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002063; ENSMUSP00000002063; ENSMUSG00000001998.
GeneIDi108011.
KEGGimmu:108011.
UCSCiuc008men.1. mouse.

Organism-specific databases

CTDi23431.
MGIiMGI:1336993. Ap4e1.

Phylogenomic databases

eggNOGiKOG1062. Eukaryota.
ENOG410XPKK. LUCA.
GeneTreeiENSGT00390000012618.
HOGENOMiHOG000231886.
HOVERGENiHBG050522.
InParanoidiQ80V94.
KOiK12400.
OMAiSYKIWKD.
OrthoDBiEOG769ZHR.
TreeFamiTF332488.

Enzyme and pathway databases

ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.

Miscellaneous databases

NextBioi359873.
PROiQ80V94.
SOURCEiSearch...

Gene expression databases

BgeeiQ80V94.
CleanExiMM_AP4E1.
ExpressionAtlasiQ80V94. baseline and differential.
GenevisibleiQ80V94. MM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR017109. AP4_complex_esu.
IPR028269. AP4E1_C.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PANTHERiPTHR22780:SF13. PTHR22780:SF13. 2 hits.
PfamiPF01602. Adaptin_N. 1 hit.
PF14807. AP4E_app_platf. 1 hit.
[Graphical view]
PIRSFiPIRSF037097. AP4_complex_epsilon. 1 hit.
SUPFAMiSSF48371. SSF48371. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 590-1122.
    Strain: FVB/NImported.
    Tissue: Mammary glandImported.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiAP4E1_MOUSE
AccessioniPrimary (citable) accession number: Q80V94
Secondary accession number(s): A2ASB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 27, 2011
Last modified: March 16, 2016
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.