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Q80V26

- IMPA3_MOUSE

UniProt

Q80V26 - IMPA3_MOUSE

Protein

Inositol monophosphatase 3

Gene

Impad1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation.1 Publication

    Catalytic activityi

    Myo-inositol phosphate + H2O = myo-inositol + phosphate.1 Publication
    Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.1 Publication

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Strongly inhibited by lithium.1 Publication

    Kineticsi

    1. KM=21 µM for 3'-phosphoadenosine 5'- phosphate (PAP)1 Publication

    Vmax=9.5 µmol/min/mg enzyme1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi131 – 1311Magnesium 1By similarity
    Binding sitei131 – 1311SubstrateBy similarity
    Metal bindingi172 – 1721Magnesium 1By similarity
    Metal bindingi172 – 1721Magnesium 2By similarity
    Metal bindingi174 – 1741Magnesium 1; via carbonyl oxygenBy similarity
    Metal bindingi175 – 1751Magnesium 2By similarity
    Metal bindingi298 – 2981Magnesium 2By similarity
    Binding sitei298 – 2981SubstrateBy similarity

    GO - Molecular functioni

    1. 3'(2'),5'-bisphosphate nucleotidase activity Source: UniProtKB-EC
    2. 3'-nucleotidase activity Source: MGI
    3. inositol monophosphate 1-phosphatase activity Source: UniProtKB-EC
    4. inositol monophosphate 3-phosphatase activity Source: UniProtKB-EC
    5. inositol monophosphate 4-phosphatase activity Source: UniProtKB-EC
    6. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. chondrocyte development Source: MGI
    2. chondroitin sulfate metabolic process Source: MGI
    3. dephosphorylation Source: GOC
    4. embryonic digit morphogenesis Source: MGI
    5. endochondral ossification Source: MGI
    6. inositol biosynthetic process Source: UniProtKB-UniPathway
    7. phosphatidylinositol phosphorylation Source: InterPro
    8. post-embryonic development Source: MGI
    9. skeletal system development Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00823; UER00788.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol monophosphatase 3 (EC:3.1.3.25, EC:3.1.3.7)
    Short name:
    IMP 3
    Short name:
    IMPase 3
    Alternative name(s):
    Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase
    Short name:
    Golgi-resident PAP phosphatase
    Short name:
    gPAPP
    Inositol monophosphatase domain-containing protein 1
    Inositol-1(or 4)-monophosphatase 3
    Myo-inositol monophosphatase A3
    Gene namesi
    Name:Impad1
    Synonyms:Impa3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1915720. Impad1.

    Subcellular locationi

    Golgi apparatus 1 Publication. Golgi apparatustrans-Golgi network membrane By similarity; Single-pass type II membrane protein By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: MGI
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mutant animals experience severe respiratory distress and died within minutes after birth. At 18.5 dpc, lungs exhibit small alveolar spaces and thickened septa. The rib cage cartilage is hypocellular with abnormal, fibrous-appearing extracellular matrix. Animals show severe skeletal abnormalities, most notably in the longitudinal growth of bones formed by endochondral ossification. The length of the axial skeleton is reduced. The appendicular bones of the upper limbs, as well as the ilium, femur, tibia and fibula of the lower limbs are markedly shorter than in wild-type littermates. The rib cages display malformation characterized by reduced sternal length and correspondingly diminished rib spacing. The process of intramembranous ossification is normal. Mutant animals exhibit a deficiency in glycosaminoglycan sulfation. Mutant cartilage and lung exhibit a substantial decrease in chondroitin 4-sulfate and an increase in nonsulfated chondroitin compared with wild type tissue.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356Inositol monophosphatase 3PRO_0000289042Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Contains N-linked glycan resistant to endoglycosydase H.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ80V26.
    PaxDbiQ80V26.
    PRIDEiQ80V26.

    PTM databases

    PhosphoSiteiQ80V26.

    Expressioni

    Developmental stagei

    At 18.5 dpc, widely expressed with enhanced levels in brain, spinal cord, lung and kidney, including medulla and cortex. In the developing brain, strongly expressed in the neopallial cortex and throughout the cerebellum with intense expression within the developing Purkinje cells and adjacent choroid plexus. Strong expression also observed within the pons and throughout the medulla oblongata. In the lung, expressed in individual pneumocytes and particularly in cells surrounding developing bronchi/bronchioles. Moderate expression in chondrocytes of costal cartilage and in the surrounding perichondrium.1 Publication

    Gene expression databases

    BgeeiQ80V26.
    CleanExiMM_IMPAD1.
    GenevestigatoriQ80V26.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000082013.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80V26.
    SMRiQ80V26. Positions 61-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini34 – 356323LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni174 – 1774Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the inositol monophosphatase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1218.
    GeneTreeiENSGT00530000063462.
    HOGENOMiHOG000290671.
    HOVERGENiHBG062091.
    InParanoidiA6H6P6.
    KOiK15759.
    OMAiNVQINTE.
    OrthoDBiEOG7CG71H.
    PhylomeDBiQ80V26.
    TreeFamiTF314300.

    Family and domain databases

    InterProiIPR000760. Inositol_monophosphatase.
    IPR020550. Inositol_monophosphatase_CS.
    [Graphical view]
    PANTHERiPTHR20854. PTHR20854. 1 hit.
    PfamiPF00459. Inositol_P. 1 hit.
    [Graphical view]
    PROSITEiPS00630. IMP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80V26-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPMGIRLSP LGVAVFFLLG LGVLYHLYSG FLAGRFSLFG LGSEPAAGEA    50
    EVASDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA 100
    DDKMTSGDVL SNRKMFYLLK TAFPNVQINT EEHVDASDKE VIVWNRKIPE 150
    DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP 200
    VLGVIHKPFS EYTAWAMVDG GSNVKARSSY NEKTPKIIVS RSHAGMVKQV 250
    ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC 300
    AGNAILKALG GHMTTLNGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD 350
    LEKSGH 356
    Length:356
    Mass (Da):38,616
    Last modified:June 1, 2003 - v1
    Checksum:iF01BF0082F5C7C82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL772346 Genomic DNA. Translation: CAM23643.1.
    CH466538 Genomic DNA. Translation: EDL05698.1.
    BC048776 mRNA. Translation: AAH48776.1.
    BC138209 mRNA. Translation: AAI38210.1.
    BC145952 mRNA. Translation: AAI45953.1.
    CCDSiCCDS17947.1.
    RefSeqiNP_808398.1. NM_177730.3.
    UniGeneiMm.218889.
    Mm.369779.

    Genome annotation databases

    EnsembliENSMUST00000084949; ENSMUSP00000082013; ENSMUSG00000066324.
    GeneIDi242291.
    KEGGimmu:242291.
    UCSCiuc008rxc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL772346 Genomic DNA. Translation: CAM23643.1 .
    CH466538 Genomic DNA. Translation: EDL05698.1 .
    BC048776 mRNA. Translation: AAH48776.1 .
    BC138209 mRNA. Translation: AAI38210.1 .
    BC145952 mRNA. Translation: AAI45953.1 .
    CCDSi CCDS17947.1.
    RefSeqi NP_808398.1. NM_177730.3.
    UniGenei Mm.218889.
    Mm.369779.

    3D structure databases

    ProteinModelPortali Q80V26.
    SMRi Q80V26. Positions 61-355.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000082013.

    PTM databases

    PhosphoSitei Q80V26.

    Proteomic databases

    MaxQBi Q80V26.
    PaxDbi Q80V26.
    PRIDEi Q80V26.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000084949 ; ENSMUSP00000082013 ; ENSMUSG00000066324 .
    GeneIDi 242291.
    KEGGi mmu:242291.
    UCSCi uc008rxc.2. mouse.

    Organism-specific databases

    CTDi 54928.
    MGIi MGI:1915720. Impad1.

    Phylogenomic databases

    eggNOGi COG1218.
    GeneTreei ENSGT00530000063462.
    HOGENOMi HOG000290671.
    HOVERGENi HBG062091.
    InParanoidi A6H6P6.
    KOi K15759.
    OMAi NVQINTE.
    OrthoDBi EOG7CG71H.
    PhylomeDBi Q80V26.
    TreeFami TF314300.

    Enzyme and pathway databases

    UniPathwayi UPA00823 ; UER00788 .

    Miscellaneous databases

    ChiTaRSi IMPAD1. mouse.
    NextBioi 385292.
    PROi Q80V26.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q80V26.
    CleanExi MM_IMPAD1.
    Genevestigatori Q80V26.

    Family and domain databases

    InterProi IPR000760. Inositol_monophosphatase.
    IPR020550. Inositol_monophosphatase_CS.
    [Graphical view ]
    PANTHERi PTHR20854. PTHR20854. 1 hit.
    Pfami PF00459. Inositol_P. 1 hit.
    [Graphical view ]
    PROSITEi PS00630. IMP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Mammary tumor.
    4. "A role for a lithium-inhibited Golgi nucleotidase in skeletal development and sulfation."
      Frederick J.P., Tafari A.T., Wu S.M., Megosh L.C., Chiou S.T., Irving R.P., York J.D.
      Proc. Natl. Acad. Sci. U.S.A. 105:11605-11612(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TOPOLOGY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiIMPA3_MOUSE
    AccessioniPrimary (citable) accession number: Q80V26
    Secondary accession number(s): A6H6P6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3