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Protein

Inositol monophosphatase 3

Gene

Impad1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation.1 Publication

Catalytic activityi

Myo-inositol phosphate + H2O = myo-inositol + phosphate.1 Publication
Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.1 Publication

Cofactori

Mg2+By similarity

Enzyme regulationi

Strongly inhibited by lithium.1 Publication

Kineticsi

  1. KM=21 µM for 3'-phosphoadenosine 5'- phosphate (PAP)1 Publication

Vmax=9.5 µmol/min/mg enzyme1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi131 – 1311Magnesium 1By similarity
Binding sitei131 – 1311SubstrateBy similarity
Metal bindingi172 – 1721Magnesium 1By similarity
Metal bindingi172 – 1721Magnesium 2By similarity
Metal bindingi174 – 1741Magnesium 1; via carbonyl oxygenBy similarity
Metal bindingi175 – 1751Magnesium 2By similarity
Metal bindingi298 – 2981Magnesium 2By similarity
Binding sitei298 – 2981SubstrateBy similarity

GO - Molecular functioni

  1. 3'(2'),5'-bisphosphate nucleotidase activity Source: UniProtKB-EC
  2. 3'-nucleotidase activity Source: MGI
  3. inositol monophosphate 1-phosphatase activity Source: UniProtKB-EC
  4. inositol monophosphate 3-phosphatase activity Source: UniProtKB-EC
  5. inositol monophosphate 4-phosphatase activity Source: UniProtKB-EC
  6. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. chondrocyte development Source: MGI
  2. chondroitin sulfate metabolic process Source: MGI
  3. dephosphorylation Source: GOC
  4. embryonic digit morphogenesis Source: MGI
  5. endochondral ossification Source: MGI
  6. inositol biosynthetic process Source: UniProtKB-UniPathway
  7. phosphatidylinositol phosphorylation Source: InterPro
  8. post-embryonic development Source: MGI
  9. skeletal system development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00823; UER00788.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol monophosphatase 3 (EC:3.1.3.25, EC:3.1.3.7)
Short name:
IMP 3
Short name:
IMPase 3
Alternative name(s):
Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase
Short name:
Golgi-resident PAP phosphatase
Short name:
gPAPP
Inositol monophosphatase domain-containing protein 1
Inositol-1(or 4)-monophosphatase 3
Myo-inositol monophosphatase A3
Gene namesi
Name:Impad1
Synonyms:Impa3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1915720. Impad1.

Subcellular locationi

Golgi apparatus 1 Publication. Golgi apparatustrans-Golgi network membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST
Topological domaini34 – 356323LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant animals experience severe respiratory distress and died within minutes after birth. At 18.5 dpc, lungs exhibit small alveolar spaces and thickened septa. The rib cage cartilage is hypocellular with abnormal, fibrous-appearing extracellular matrix. Animals show severe skeletal abnormalities, most notably in the longitudinal growth of bones formed by endochondral ossification. The length of the axial skeleton is reduced. The appendicular bones of the upper limbs, as well as the ilium, femur, tibia and fibula of the lower limbs are markedly shorter than in wild-type littermates. The rib cages display malformation characterized by reduced sternal length and correspondingly diminished rib spacing. The process of intramembranous ossification is normal. Mutant animals exhibit a deficiency in glycosaminoglycan sulfation. Mutant cartilage and lung exhibit a substantial decrease in chondroitin 4-sulfate and an increase in nonsulfated chondroitin compared with wild type tissue.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Inositol monophosphatase 3PRO_0000289042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Contains N-linked glycan resistant to endoglycosydase H.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ80V26.
PaxDbiQ80V26.
PRIDEiQ80V26.

PTM databases

PhosphoSiteiQ80V26.

Expressioni

Developmental stagei

At 18.5 dpc, widely expressed with enhanced levels in brain, spinal cord, lung and kidney, including medulla and cortex. In the developing brain, strongly expressed in the neopallial cortex and throughout the cerebellum with intense expression within the developing Purkinje cells and adjacent choroid plexus. Strong expression also observed within the pons and throughout the medulla oblongata. In the lung, expressed in individual pneumocytes and particularly in cells surrounding developing bronchi/bronchioles. Moderate expression in chondrocytes of costal cartilage and in the surrounding perichondrium.1 Publication

Gene expression databases

BgeeiQ80V26.
CleanExiMM_IMPAD1.
GenevestigatoriQ80V26.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082013.

Structurei

3D structure databases

ProteinModelPortaliQ80V26.
SMRiQ80V26. Positions 61-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni174 – 1774Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the inositol monophosphatase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1218.
GeneTreeiENSGT00530000063462.
HOGENOMiHOG000290671.
HOVERGENiHBG062091.
InParanoidiQ80V26.
KOiK15759.
OMAiNVQINTE.
OrthoDBiEOG7CG71H.
PhylomeDBiQ80V26.
TreeFamiTF314300.

Family and domain databases

InterProiIPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PROSITEiPS00630. IMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80V26-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPMGIRLSP LGVAVFFLLG LGVLYHLYSG FLAGRFSLFG LGSEPAAGEA
60 70 80 90 100
EVASDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA
110 120 130 140 150
DDKMTSGDVL SNRKMFYLLK TAFPNVQINT EEHVDASDKE VIVWNRKIPE
160 170 180 190 200
DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP
210 220 230 240 250
VLGVIHKPFS EYTAWAMVDG GSNVKARSSY NEKTPKIIVS RSHAGMVKQV
260 270 280 290 300
ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC
310 320 330 340 350
AGNAILKALG GHMTTLNGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD

LEKSGH
Length:356
Mass (Da):38,616
Last modified:June 1, 2003 - v1
Checksum:iF01BF0082F5C7C82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL772346 Genomic DNA. Translation: CAM23643.1.
CH466538 Genomic DNA. Translation: EDL05698.1.
BC048776 mRNA. Translation: AAH48776.1.
BC138209 mRNA. Translation: AAI38210.1.
BC145952 mRNA. Translation: AAI45953.1.
CCDSiCCDS17947.1.
RefSeqiNP_808398.1. NM_177730.3.
UniGeneiMm.218889.
Mm.369779.

Genome annotation databases

EnsembliENSMUST00000084949; ENSMUSP00000082013; ENSMUSG00000066324.
GeneIDi242291.
KEGGimmu:242291.
UCSCiuc008rxc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL772346 Genomic DNA. Translation: CAM23643.1.
CH466538 Genomic DNA. Translation: EDL05698.1.
BC048776 mRNA. Translation: AAH48776.1.
BC138209 mRNA. Translation: AAI38210.1.
BC145952 mRNA. Translation: AAI45953.1.
CCDSiCCDS17947.1.
RefSeqiNP_808398.1. NM_177730.3.
UniGeneiMm.218889.
Mm.369779.

3D structure databases

ProteinModelPortaliQ80V26.
SMRiQ80V26. Positions 61-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082013.

PTM databases

PhosphoSiteiQ80V26.

Proteomic databases

MaxQBiQ80V26.
PaxDbiQ80V26.
PRIDEiQ80V26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084949; ENSMUSP00000082013; ENSMUSG00000066324.
GeneIDi242291.
KEGGimmu:242291.
UCSCiuc008rxc.2. mouse.

Organism-specific databases

CTDi54928.
MGIiMGI:1915720. Impad1.

Phylogenomic databases

eggNOGiCOG1218.
GeneTreeiENSGT00530000063462.
HOGENOMiHOG000290671.
HOVERGENiHBG062091.
InParanoidiQ80V26.
KOiK15759.
OMAiNVQINTE.
OrthoDBiEOG7CG71H.
PhylomeDBiQ80V26.
TreeFamiTF314300.

Enzyme and pathway databases

UniPathwayiUPA00823; UER00788.

Miscellaneous databases

ChiTaRSiImpad1. mouse.
NextBioi385292.
PROiQ80V26.
SOURCEiSearch...

Gene expression databases

BgeeiQ80V26.
CleanExiMM_IMPAD1.
GenevestigatoriQ80V26.

Family and domain databases

InterProiIPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PROSITEiPS00630. IMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  4. "A role for a lithium-inhibited Golgi nucleotidase in skeletal development and sulfation."
    Frederick J.P., Tafari A.T., Wu S.M., Megosh L.C., Chiou S.T., Irving R.P., York J.D.
    Proc. Natl. Acad. Sci. U.S.A. 105:11605-11612(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TOPOLOGY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiIMPA3_MOUSE
AccessioniPrimary (citable) accession number: Q80V26
Secondary accession number(s): A6H6P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2003
Last modified: February 4, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.