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Protein

E3 ubiquitin-protein ligase KCMF1

Gene

Kcmf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3 – 50ZZ-typePROSITE-ProRule annotationAdd BLAST48
Zinc fingeri78 – 101C2H2-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase KCMF1 (EC:2.3.2.27)
Alternative name(s):
Differentially expressed in branching tubulogenesis 91
Short name:
Debt-91
RING-type E3 ubiquitin transferase KCMF1Curated
Gene namesi
Name:Kcmf1
Synonyms:Debt91
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1921537 Kcmf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003492202 – 381E3 ubiquitin-protein ligase KCMF1Add BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei169PhosphoserineBy similarity1
Modified residuei189PhosphoserineBy similarity1
Modified residuei212PhosphoserineBy similarity1
Modified residuei335PhosphoserineBy similarity1
Modified residuei336PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ80UY2
PaxDbiQ80UY2
PeptideAtlasiQ80UY2
PRIDEiQ80UY2

PTM databases

iPTMnetiQ80UY2
PhosphoSitePlusiQ80UY2

Expressioni

Tissue specificityi

Testis, liver, kidney, heart and skeletal muscle.1 Publication

Developmental stagei

Expressed throughout embryonic development, with much higher expression after day 15, when many organs including the kidney are undergoing extensive branching morphogenesis.1 Publication

Inductioni

Up-regulated during growth factor-induced branching tubulogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000055239
ExpressionAtlasiQ80UY2 baseline and differential
GenevisibleiQ80UY2 MM

Interactioni

Protein-protein interaction databases

IntActiQ80UY2, 1 interactor
STRINGi10090.ENSMUSP00000064410

Structurei

3D structure databases

ProteinModelPortaliQ80UY2
SMRiQ80UY2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili224 – 259Sequence analysisAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi333 – 336Poly-Ser4
Compositional biasi376 – 380Poly-Pro5

Sequence similaritiesi

Belongs to the KCMF1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3 – 50ZZ-typePROSITE-ProRule annotationAdd BLAST48
Zinc fingeri78 – 101C2H2-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1280 Eukaryota
ENOG410XRX5 LUCA
GeneTreeiENSGT00510000047171
HOGENOMiHOG000007727
HOVERGENiHBG101229
InParanoidiQ80UY2
KOiK22376
OMAiAVDGRNQ
OrthoDBiEOG091G09BU
PhylomeDBiQ80UY2
TreeFamiTF318128

Family and domain databases

InterProiView protein in InterPro
IPR008598 Di19_Zn_binding_dom
IPR013087 Znf_C2H2_type
IPR000433 Znf_ZZ
PfamiView protein in Pfam
PF05605 zf-Di19, 1 hit
PF00569 ZZ, 1 hit
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 1 hit
SM00291 ZnF_ZZ, 1 hit
PROSITEiView protein in PROSITE
PS01357 ZF_ZZ_1, 1 hit
PS50135 ZF_ZZ_2, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80UY2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRHEGVSCD ACLKGNFRGR RYKCLICYDY DLCASCYESG ATTTRHTTDH
60 70 80 90 100
PMQCILTRVD FDLYYGGEAF SVEQPQSFTC PYCGKMGYTE TSLQEHVTSE
110 120 130 140 150
HAETSTEVIC PICAALPGGD PNHVTDDFAA HLTLEHRAPR DLDESSGVRH
160 170 180 190 200
VRRMFHPGRG LGGPRARRSN MHFTSSSTGG LSSSQSSYSP SSREAMDPIA
210 220 230 240 250
ELLSQLSGVR RSAGGQLNSS GPSASQLQQL QMQLQLERQH AQAARQQLET
260 270 280 290 300
ARNASRRTNT SSVTTTITQA TATANTANTE NSPQALHNSQ FLLTRLNDPK
310 320 330 340 350
MSEAERQSME SERADRSLFV QELLLSTLVR EESSSSDEDD RGEMADFGAM
360 370 380
GCVDIMPLDV ALENLNLKES NKGNEPPPPP L
Length:381
Mass (Da):41,791
Last modified:June 1, 2003 - v1
Checksum:i4A15FC5CF60CF246
GO
Isoform 2 (identifier: Q80UY2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.

Show »
Length:296
Mass (Da):32,136
Checksum:iFED76A8CC957201B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0352271 – 85Missing in isoform 2. 1 PublicationAdd BLAST85

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143859 mRNA Translation: AAD31040.1
AK142436 mRNA Translation: BAE25063.1
BC043330 mRNA Translation: AAH43330.1
CCDSiCCDS39518.1 [Q80UY2-1]
RefSeqiNP_062689.2, NM_019715.2 [Q80UY2-1]
UniGeneiMm.29194

Genome annotation databases

EnsembliENSMUST00000068697; ENSMUSP00000064410; ENSMUSG00000055239 [Q80UY2-1]
GeneIDi74287
KEGGimmu:74287
UCSCiuc009cjd.1 mouse [Q80UY2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKCMF1_MOUSE
AccessioniPrimary (citable) accession number: Q80UY2
Secondary accession number(s): Q9WUM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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