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Protein

E3 ubiquitin-protein ligase KCMF1

Gene

Kcmf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 5048ZZ-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri78 – 10124C2H2-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase KCMF1 (EC:6.3.2.-)
Alternative name(s):
Differentially expressed in branching tubulogenesis 91
Short name:
Debt-91
Gene namesi
Name:Kcmf1
Synonyms:Debt91
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1921537. Kcmf1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 381380E3 ubiquitin-protein ligase KCMF1PRO_0000349220Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei169 – 1691PhosphoserineBy similarity
Modified residuei189 – 1891PhosphoserineBy similarity
Modified residuei212 – 2121PhosphoserineBy similarity
Modified residuei335 – 3351PhosphoserineBy similarity
Modified residuei336 – 3361PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ80UY2.
MaxQBiQ80UY2.
PaxDbiQ80UY2.
PeptideAtlasiQ80UY2.
PRIDEiQ80UY2.

PTM databases

iPTMnetiQ80UY2.
PhosphoSiteiQ80UY2.

Expressioni

Tissue specificityi

Testis, liver, kidney, heart and skeletal muscle.1 Publication

Developmental stagei

Expressed throughout embryonic development, with much higher expression after day 15, when many organs including the kidney are undergoing extensive branching morphogenesis.1 Publication

Inductioni

Up-regulated during growth factor-induced branching tubulogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000055239.
ExpressionAtlasiQ80UY2. baseline and differential.
GenevisibleiQ80UY2. MM.

Interactioni

Protein-protein interaction databases

IntActiQ80UY2. 1 interaction.
MINTiMINT-1675785.
STRINGi10090.ENSMUSP00000064410.

Structurei

3D structure databases

ProteinModelPortaliQ80UY2.
SMRiQ80UY2. Positions 2-59.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili224 – 25936Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi333 – 3364Poly-Ser
Compositional biasi376 – 3805Poly-Pro

Sequence similaritiesi

Belongs to the KCMF1 family.Curated
Contains 1 C2H2-type zinc finger.PROSITE-ProRule annotation
Contains 1 ZZ-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 5048ZZ-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri78 – 10124C2H2-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1280. Eukaryota.
ENOG410XRX5. LUCA.
GeneTreeiENSGT00510000047171.
HOGENOMiHOG000007727.
HOVERGENiHBG101229.
InParanoidiQ80UY2.
OMAiIGVSCDA.
OrthoDBiEOG091G09BU.
PhylomeDBiQ80UY2.
TreeFamiTF318128.

Family and domain databases

InterProiIPR008598. Di19_Zn_binding_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR000433. Znf_ZZ.
[Graphical view]
PfamiPF05605. zf-Di19. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
PROSITEiPS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80UY2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRHEGVSCD ACLKGNFRGR RYKCLICYDY DLCASCYESG ATTTRHTTDH
60 70 80 90 100
PMQCILTRVD FDLYYGGEAF SVEQPQSFTC PYCGKMGYTE TSLQEHVTSE
110 120 130 140 150
HAETSTEVIC PICAALPGGD PNHVTDDFAA HLTLEHRAPR DLDESSGVRH
160 170 180 190 200
VRRMFHPGRG LGGPRARRSN MHFTSSSTGG LSSSQSSYSP SSREAMDPIA
210 220 230 240 250
ELLSQLSGVR RSAGGQLNSS GPSASQLQQL QMQLQLERQH AQAARQQLET
260 270 280 290 300
ARNASRRTNT SSVTTTITQA TATANTANTE NSPQALHNSQ FLLTRLNDPK
310 320 330 340 350
MSEAERQSME SERADRSLFV QELLLSTLVR EESSSSDEDD RGEMADFGAM
360 370 380
GCVDIMPLDV ALENLNLKES NKGNEPPPPP L
Length:381
Mass (Da):41,791
Last modified:June 1, 2003 - v1
Checksum:i4A15FC5CF60CF246
GO
Isoform 2 (identifier: Q80UY2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.

Show »
Length:296
Mass (Da):32,136
Checksum:iFED76A8CC957201B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8585Missing in isoform 2. 1 PublicationVSP_035227Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143859 mRNA. Translation: AAD31040.1.
AK142436 mRNA. Translation: BAE25063.1.
BC043330 mRNA. Translation: AAH43330.1.
CCDSiCCDS39518.1. [Q80UY2-1]
RefSeqiNP_062689.2. NM_019715.2. [Q80UY2-1]
UniGeneiMm.29194.

Genome annotation databases

EnsembliENSMUST00000068697; ENSMUSP00000064410; ENSMUSG00000055239. [Q80UY2-1]
GeneIDi74287.
KEGGimmu:74287.
UCSCiuc009cjd.1. mouse. [Q80UY2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143859 mRNA. Translation: AAD31040.1.
AK142436 mRNA. Translation: BAE25063.1.
BC043330 mRNA. Translation: AAH43330.1.
CCDSiCCDS39518.1. [Q80UY2-1]
RefSeqiNP_062689.2. NM_019715.2. [Q80UY2-1]
UniGeneiMm.29194.

3D structure databases

ProteinModelPortaliQ80UY2.
SMRiQ80UY2. Positions 2-59.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80UY2. 1 interaction.
MINTiMINT-1675785.
STRINGi10090.ENSMUSP00000064410.

PTM databases

iPTMnetiQ80UY2.
PhosphoSiteiQ80UY2.

Proteomic databases

EPDiQ80UY2.
MaxQBiQ80UY2.
PaxDbiQ80UY2.
PeptideAtlasiQ80UY2.
PRIDEiQ80UY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068697; ENSMUSP00000064410; ENSMUSG00000055239. [Q80UY2-1]
GeneIDi74287.
KEGGimmu:74287.
UCSCiuc009cjd.1. mouse. [Q80UY2-1]

Organism-specific databases

CTDi56888.
MGIiMGI:1921537. Kcmf1.

Phylogenomic databases

eggNOGiKOG1280. Eukaryota.
ENOG410XRX5. LUCA.
GeneTreeiENSGT00510000047171.
HOGENOMiHOG000007727.
HOVERGENiHBG101229.
InParanoidiQ80UY2.
OMAiIGVSCDA.
OrthoDBiEOG091G09BU.
PhylomeDBiQ80UY2.
TreeFamiTF318128.

Miscellaneous databases

ChiTaRSiKcmf1. mouse.
PROiQ80UY2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000055239.
ExpressionAtlasiQ80UY2. baseline and differential.
GenevisibleiQ80UY2. MM.

Family and domain databases

InterProiIPR008598. Di19_Zn_binding_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR000433. Znf_ZZ.
[Graphical view]
PfamiPF05605. zf-Di19. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
PROSITEiPS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCMF1_MOUSE
AccessioniPrimary (citable) accession number: Q80UY2
Secondary accession number(s): Q9WUM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.